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The map label for this gene is spoT [H]

Identifier: 93006829

GI number: 93006829

Start: 2464858

End: 2467044

Strand: Direct

Name: spoT [H]

Synonym: Pcryo_2005

Alternate gene names: 93006829

Gene position: 2464858-2467044 (Clockwise)

Preceding gene: 93006828

Following gene: 93006830

Centisome position: 80.55

GC content: 43.26

Gene sequence:

>2187_bases
ATGATTCAAAACTTGCCCAAACTCAGTCATCCGTTAGTTGATGACGCTCAATATAACCTGCTGCGTTCAGTAGGTTACCT
CACGGGCGCTGAACGCCGTGATATTGTTGATGCCTGTGAGTTCGGTGATGTTGCGCATATTAAAGACAAGCGCAAGTCAG
GAGAGCCTTATATCACGCATCCGATTGCGGTCGCTGAGATACTAGCCGGCTTTCGCTTAGATCGAGATACCATCATTGCG
GCAATTTTGCATGATACGGTTGAAGATACGGAAGTGAGCGATGAGCAGATAGAGCAGCGTTATGGCAAAGTGGTTTCTAG
GCTGGTCGATGGTGTGACCAAATTAAAATCATCGACGCATAATAAACAAGAAAATAAAGCGGCGACCTTTCATAAAATCC
TAACGGCTACCCTCGCAGACCCACGTGTATTGATTATCAAGCTTGCTGACCGTTTGCACAATATGTCGACCCTAGATGCG
GTACGTCCTGAAAAACAACGTACCACCGCTCAAGAAACTTTAGATTTTTATGTGCCCTTTGCACGGCTCATGGGTCTCAA
CGATATTGCTGATTATATCGAGGTACTTTGTTATCGCAATCTTAACTCTGAGATGTATAACAAGATCTCTGATAAATTGC
TACAACATGGGCTTGGGCGCAATTTTCAGAGAGAAGCCATTCATCGTTATTTAAGTATTGTTCTCAATAATCTTGATCTT
GATGGCATGGTTAAAGTCTTAGACAATCGCGTGGTTATCTTCCGACAATTTTTCCGTAATCGCGGCGAGATTAATGCACT
GCTACGCCATTACGCATTTGAGATTGTGCTAGATAATATCGAAGCTTGTGACAAACTTGCCTACTATCTCATTAAAAAAT
ATCAAATTGCGGACAGTCATATCGCGGACAATATCCGCAGACCTTTACCTGGCGGCAATCAATCGCTGACGCTTATCTAC
GAGCGCGACAATGATTTTGTTAAAGTAACGATTTTGACTAAGCAGATGCAAAGTGCTGCAAGGCTTGGGGTTATTGGTGC
AGAGCATGCCTCTGATGTCAGTCAATCAGTCATTCAGGCGTCATTACGCAATATGAAAGATTTGGTCGATGAGGACTGTT
TAAACGAAGGCAATCCTGATTTTGCCACCGCGGTATCAACCATTAATGAGCTGATGGATTACCTGCATTCTAGCAAAATT
ATCTGTTATAGCCCGCAAGGTCGCGCTTATGAGTTGCCACAAGGTGCGACTGCGCTAGACTTTGCTTATGCTGTTGGTCC
GATGGTTGGCAATGTCGCGGTTGGCGCTAATATTGATAAAAAACATGCCAAGCTTGGGACGGTAGTCAAAAATGGACAGT
TAGTAGAAATCGAGGTTAATAGCCATTCTGAGCCAAAAGCAGAATGGCTTGGGTTTGTGGTTACTAATAAAGCCCGTGTA
GAGATTTTGCGTTGGTTCAAAGATTTATCGGCGGCTGATAAGCAGCACCATGGTAGACAAGCACTAGATCGCGCACTGAA
AACTTATCAAAAAAGCTTGGACGATCTAACCGATAGTGACTGGAAGAATCTAACAGAATGGCGTGGACTGACTGAGAAAT
CAGCGCTATTTGAGCAAATAAGCTCCGGTACGTTGTTACCGCAACTCGTGGTCACGCGTTTGTTTAGCGATGAAGTCTGT
GATATCCAATCGCAAGAACGTATCGATGACATGACCCAACCACAGCAACTGATCGTCAATGCTTCAGGCGTTGAGCTTGA
TTTTGCCAATTGTTGCCATCCGATTTATGGTGATCCTATCGTTGGACATTTATCCCGTCATGGTCTGGTCGTGCATCGAC
ATAAGTGCTTTTCACTAGACGATATTCGTAAAGACAATCCCTATCAAGTCATCCAATTACGTTGGCGCAATGATAAGGCG
ATCAAGCAAAGTGACTCAGAAGATCACGATAATAAAATTCGTTTTCCTGCTTATTTAAAACTGTCTATAGCACTGAGCGA
CGAGCAAATCAGTAAAGTTATTTATAATCTGCGGCAGTTAAATATAGGGGTTGAAAAGGTCGACGTACGTAGCAATGATA
CTATCATTCATATTGTTGTTCGTAGTCGCAATCACTTAGCCCAAGGTATTCGTGAACTACGCTCCTTACTTGGTTTCCCC
AATATTATGCGTTTATATCAACTATAA

Upstream 100 bases:

>100_bases
ATTCTTAAGCTCTATATCAATAGCTCAGCATTTATTCAGCCATTCTTTACCTTGCTAAATCATCATTTAAGCAGGTAAAA
GAACATAGGATCGTGACTTT

Downstream 100 bases:

>100_bases
ATACTTTTATCATCATAACTGTTAAAATATATGCCTTTTAAATTATCTCTTAACACATAGATGATTTTGAAATGTGTAAA
ACTTTTAATCGTGCTAAACA

Product: metal dependent phosphohydrolase

Products: NA

Alternate protein names: Penta-phosphate guanosine-3'-pyrophosphohydrolase; (ppGpp)ase [H]

Number of amino acids: Translated: 728; Mature: 728

Protein sequence:

>728_residues
MIQNLPKLSHPLVDDAQYNLLRSVGYLTGAERRDIVDACEFGDVAHIKDKRKSGEPYITHPIAVAEILAGFRLDRDTIIA
AILHDTVEDTEVSDEQIEQRYGKVVSRLVDGVTKLKSSTHNKQENKAATFHKILTATLADPRVLIIKLADRLHNMSTLDA
VRPEKQRTTAQETLDFYVPFARLMGLNDIADYIEVLCYRNLNSEMYNKISDKLLQHGLGRNFQREAIHRYLSIVLNNLDL
DGMVKVLDNRVVIFRQFFRNRGEINALLRHYAFEIVLDNIEACDKLAYYLIKKYQIADSHIADNIRRPLPGGNQSLTLIY
ERDNDFVKVTILTKQMQSAARLGVIGAEHASDVSQSVIQASLRNMKDLVDEDCLNEGNPDFATAVSTINELMDYLHSSKI
ICYSPQGRAYELPQGATALDFAYAVGPMVGNVAVGANIDKKHAKLGTVVKNGQLVEIEVNSHSEPKAEWLGFVVTNKARV
EILRWFKDLSAADKQHHGRQALDRALKTYQKSLDDLTDSDWKNLTEWRGLTEKSALFEQISSGTLLPQLVVTRLFSDEVC
DIQSQERIDDMTQPQQLIVNASGVELDFANCCHPIYGDPIVGHLSRHGLVVHRHKCFSLDDIRKDNPYQVIQLRWRNDKA
IKQSDSEDHDNKIRFPAYLKLSIALSDEQISKVIYNLRQLNIGVEKVDVRSNDTIIHIVVRSRNHLAQGIRELRSLLGFP
NIMRLYQL

Sequences:

>Translated_728_residues
MIQNLPKLSHPLVDDAQYNLLRSVGYLTGAERRDIVDACEFGDVAHIKDKRKSGEPYITHPIAVAEILAGFRLDRDTIIA
AILHDTVEDTEVSDEQIEQRYGKVVSRLVDGVTKLKSSTHNKQENKAATFHKILTATLADPRVLIIKLADRLHNMSTLDA
VRPEKQRTTAQETLDFYVPFARLMGLNDIADYIEVLCYRNLNSEMYNKISDKLLQHGLGRNFQREAIHRYLSIVLNNLDL
DGMVKVLDNRVVIFRQFFRNRGEINALLRHYAFEIVLDNIEACDKLAYYLIKKYQIADSHIADNIRRPLPGGNQSLTLIY
ERDNDFVKVTILTKQMQSAARLGVIGAEHASDVSQSVIQASLRNMKDLVDEDCLNEGNPDFATAVSTINELMDYLHSSKI
ICYSPQGRAYELPQGATALDFAYAVGPMVGNVAVGANIDKKHAKLGTVVKNGQLVEIEVNSHSEPKAEWLGFVVTNKARV
EILRWFKDLSAADKQHHGRQALDRALKTYQKSLDDLTDSDWKNLTEWRGLTEKSALFEQISSGTLLPQLVVTRLFSDEVC
DIQSQERIDDMTQPQQLIVNASGVELDFANCCHPIYGDPIVGHLSRHGLVVHRHKCFSLDDIRKDNPYQVIQLRWRNDKA
IKQSDSEDHDNKIRFPAYLKLSIALSDEQISKVIYNLRQLNIGVEKVDVRSNDTIIHIVVRSRNHLAQGIRELRSLLGFP
NIMRLYQL
>Mature_728_residues
MIQNLPKLSHPLVDDAQYNLLRSVGYLTGAERRDIVDACEFGDVAHIKDKRKSGEPYITHPIAVAEILAGFRLDRDTIIA
AILHDTVEDTEVSDEQIEQRYGKVVSRLVDGVTKLKSSTHNKQENKAATFHKILTATLADPRVLIIKLADRLHNMSTLDA
VRPEKQRTTAQETLDFYVPFARLMGLNDIADYIEVLCYRNLNSEMYNKISDKLLQHGLGRNFQREAIHRYLSIVLNNLDL
DGMVKVLDNRVVIFRQFFRNRGEINALLRHYAFEIVLDNIEACDKLAYYLIKKYQIADSHIADNIRRPLPGGNQSLTLIY
ERDNDFVKVTILTKQMQSAARLGVIGAEHASDVSQSVIQASLRNMKDLVDEDCLNEGNPDFATAVSTINELMDYLHSSKI
ICYSPQGRAYELPQGATALDFAYAVGPMVGNVAVGANIDKKHAKLGTVVKNGQLVEIEVNSHSEPKAEWLGFVVTNKARV
EILRWFKDLSAADKQHHGRQALDRALKTYQKSLDDLTDSDWKNLTEWRGLTEKSALFEQISSGTLLPQLVVTRLFSDEVC
DIQSQERIDDMTQPQQLIVNASGVELDFANCCHPIYGDPIVGHLSRHGLVVHRHKCFSLDDIRKDNPYQVIQLRWRNDKA
IKQSDSEDHDNKIRFPAYLKLSIALSDEQISKVIYNLRQLNIGVEKVDVRSNDTIIHIVVRSRNHLAQGIRELRSLLGFP
NIMRLYQL

Specific function: In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the degradation of ppGpp into G

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HD domain [H]

Homologues:

Organism=Homo sapiens, GI38348360, Length=140, Percent_Identity=37.8571428571429, Blast_Score=77, Evalue=4e-14,
Organism=Escherichia coli, GI1790082, Length=695, Percent_Identity=31.5107913669065, Blast_Score=305, Evalue=7e-84,
Organism=Escherichia coli, GI1789147, Length=610, Percent_Identity=25.5737704918033, Blast_Score=161, Evalue=1e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012675
- InterPro:   IPR003607
- InterPro:   IPR007685
- InterPro:   IPR004811
- InterPro:   IPR004095
- InterPro:   IPR012676 [H]

Pfam domain/function: PF04607 RelA_SpoT; PF02824 TGS [H]

EC number: =3.1.7.2 [H]

Molecular weight: Translated: 82607; Mature: 82607

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQNLPKLSHPLVDDAQYNLLRSVGYLTGAERRDIVDACEFGDVAHIKDKRKSGEPYITH
CCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHCCCCCEEEC
PIAVAEILAGFRLDRDTIIAAILHDTVEDTEVSDEQIEQRYGKVVSRLVDGVTKLKSSTH
HHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
NKQENKAATFHKILTATLADPRVLIIKLADRLHNMSTLDAVRPEKQRTTAQETLDFYVPF
CCHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHCCHHHHHCCCHHHHHHHHHHHHHHHHH
ARLMGLNDIADYIEVLCYRNLNSEMYNKISDKLLQHGLGRNFQREAIHRYLSIVLNNLDL
HHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCH
DGMVKVLDNRVVIFRQFFRNRGEINALLRHYAFEIVLDNIEACDKLAYYLIKKYQIADSH
HHHHHHHHCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IADNIRRPLPGGNQSLTLIYERDNDFVKVTILTKQMQSAARLGVIGAEHASDVSQSVIQA
HHHHHCCCCCCCCCEEEEEEECCCCEEEEEEEHHHHHHHHHHCCCCCHHHHHHHHHHHHH
SLRNMKDLVDEDCLNEGNPDFATAVSTINELMDYLHSSKIICYSPQGRAYELPQGATALD
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCEEECCCCCCHHH
FAYAVGPMVGNVAVGANIDKKHAKLGTVVKNGQLVEIEVNSHSEPKAEWLGFVVTNKARV
HHHHHHHHHCCEEECCCCCHHHHHHHHEEECCEEEEEEECCCCCCHHHHHEEEEECHHHH
EILRWFKDLSAADKQHHGRQALDRALKTYQKSLDDLTDSDWKNLTEWRGLTEKSALFEQI
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHH
SSGTLLPQLVVTRLFSDEVCDIQSQERIDDMTQPQQLIVNASGVELDFANCCHPIYGDPI
HCCCHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCHHHEEECCCCEEEHHHCCCCCCCCCH
VGHLSRHGLVVHRHKCFSLDDIRKDNPYQVIQLRWRNDKAIKQSDSEDHDNKIRFPAYLK
HHHHHHCCEEEEECCCCCHHHCCCCCCEEEEEEEECCCCHHCCCCCCCCCCEEECCEEEE
LSIALSDEQISKVIYNLRQLNIGVEKVDVRSNDTIIHIVVRSRNHLAQGIRELRSLLGFP
EEEEECHHHHHHHHHHHHHHCCCCEEEEECCCCCEEEEEECCCHHHHHHHHHHHHHHCCH
NIMRLYQL
HHHHHHCC
>Mature Secondary Structure
MIQNLPKLSHPLVDDAQYNLLRSVGYLTGAERRDIVDACEFGDVAHIKDKRKSGEPYITH
CCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHCCCCCEEEC
PIAVAEILAGFRLDRDTIIAAILHDTVEDTEVSDEQIEQRYGKVVSRLVDGVTKLKSSTH
HHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
NKQENKAATFHKILTATLADPRVLIIKLADRLHNMSTLDAVRPEKQRTTAQETLDFYVPF
CCHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHCCHHHHHCCCHHHHHHHHHHHHHHHHH
ARLMGLNDIADYIEVLCYRNLNSEMYNKISDKLLQHGLGRNFQREAIHRYLSIVLNNLDL
HHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCH
DGMVKVLDNRVVIFRQFFRNRGEINALLRHYAFEIVLDNIEACDKLAYYLIKKYQIADSH
HHHHHHHHCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IADNIRRPLPGGNQSLTLIYERDNDFVKVTILTKQMQSAARLGVIGAEHASDVSQSVIQA
HHHHHCCCCCCCCCEEEEEEECCCCEEEEEEEHHHHHHHHHHCCCCCHHHHHHHHHHHHH
SLRNMKDLVDEDCLNEGNPDFATAVSTINELMDYLHSSKIICYSPQGRAYELPQGATALD
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCEEECCCCCCHHH
FAYAVGPMVGNVAVGANIDKKHAKLGTVVKNGQLVEIEVNSHSEPKAEWLGFVVTNKARV
HHHHHHHHHCCEEECCCCCHHHHHHHHEEECCEEEEEEECCCCCCHHHHHEEEEECHHHH
EILRWFKDLSAADKQHHGRQALDRALKTYQKSLDDLTDSDWKNLTEWRGLTEKSALFEQI
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHH
SSGTLLPQLVVTRLFSDEVCDIQSQERIDDMTQPQQLIVNASGVELDFANCCHPIYGDPI
HCCCHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCHHHEEECCCCEEEHHHCCCCCCCCCH
VGHLSRHGLVVHRHKCFSLDDIRKDNPYQVIQLRWRNDKAIKQSDSEDHDNKIRFPAYLK
HHHHHHCCEEEEECCCCCHHHCCCCCCEEEEEEEECCCCHHCCCCCCCCCCEEECCEEEE
LSIALSDEQISKVIYNLRQLNIGVEKVDVRSNDTIIHIVVRSRNHLAQGIRELRSLLGFP
EEEEECHHHHHHHHHHHHHHCCCCEEEEECCCCCEEEEEECCCHHHHHHHHHHHHHHCCH
NIMRLYQL
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]