Click here to switch to the map view.

The map label for this gene is eptA [H]

Identifier: 93006073

GI number: 93006073

Start: 1515753

End: 1517471

Strand: Reverse

Name: eptA [H]

Synonym: Pcryo_1245

Alternate gene names: 93006073

Gene position: 1517471-1515753 (Counterclockwise)

Preceding gene: 93006078

Following gene: 93006072

Centisome position: 49.59

GC content: 41.24

Gene sequence:

>1719_bases
ATGTCAAAACACTTAATTACCGATCTAGCCATTGCAGAAACCCATAATATAAGTCTTGCTAAAAATAATAATGCAACTTC
CGATAAAAATAAATTTTATCATCGTGAAGTCAATCTTACTTATCTGATTATAGTGGTTACGCTCTATCTCGTTGCTACTG
CAAATATTGGCTTCTTTAAACAAGTATTAACGATCTATCCGTTTGCTGATAACATTGGTTTTATCGTTTCTCTGACTGGC
TTACTGTTTGGGCTGATGTGGTTATTGTTTCAGCTATTATGTTATCGACCGACAGCAAAAGTAGTACTTATTGCTATGGT
GCTGATTGCGGCAGTATGTGGTTACTTCACCGATGCTTATGGAACGATATTTGACACTGGCATGTTGGTTAACAGCTTGC
AGACAGACCAAGCAGAAGCGATGGGTTTGATAGCACCAAGCTTTATTGTTCGACTGCTACTATTGGGTCTGCTACCTGCT
TTCTTAATTAGTAAGATTCGTATAAAGCGTTTGTCTATAGTTCGCTCAATCGTGCAAAAGACAATCACCTTGATAACGTC
TATTGCGATAATAGCGGTTTGCCTATTACCATTTGGAGATCAATACGCCAGCTTTTTCCGCCAGCATAAAATAGTACGTA
GTTATACCAATCCCATTACCCCTGTTTATTCTGTTATCAAACTCGGTACCGATTACGTCGATGAGCTACGCCGCCCTGAT
ACCTTGATACTGCATGCGCCAGATGCAAAGCGTACCGCAGCCGTCAGTAGCACAACGAAACCTAAGCTGATGGTGTTCGT
AGTCGGTGAAACGGTTCGCGCTGACCATATTGGCTTGAATGGCTATAAACGCGATACGATGCCGTTACTTGCCAGCCAGC
CGGATATCTATAGCTACAAGCATGCGACCTCTTGTGGTACGTCGACTGCTTATTCAGTACCTTGCATGTTCAGTTATGCA
AACCGAAGCGATTACGACGTTGACACTGCTGAATATAATGAAAACGTGCTTGATACCCTCAACAAACAAGGCGTTAATGT
TATCTGGCGGGACAATAACTCTAGCTCTAAAGGCGTGGCAGATCGAGTCACCGTTGAGGACTATAAAACGCCTAAGCTAA
ATCCTAATTGTGATATTGAATGTCGTGATATTGGCATGTTGGACGGTTTTGATACTTTAGTTAAGTCGGACACAGGGGTC
AAGACAGCTGCGAAGCCACAAGATACACTGATTTTATTACATCAAATGGGCAATCACGGTCCTGCTTATTATCAACGTTA
TCCCAAGGAATTTGAAGAATACAAGCCAGTCTGTATGACCAACGAGTTATCTAAGTGTGATGCTCAATCCGTTATAAATG
GTTATGACAATGCGATTCGCTATACCGATTACTTTTTAAATGCCGTCATAGATACGCTAAAACCCTACGAGCAAGACTAT
GACGTAGTGATGGTATATATTAGTGATCATGGGGAAAGTCTGGGTGAAAACAATGTATATTTACATGGTCTGCCGTACGG
TATTGCGCCGAATGCGCAAAAACAGGTGCCAGTCATTATTTGGTCACCTAAAAACAATGGCATCAATCCTAAGAGTCTGT
CTAATATGACCAATCAGCCAGTGTCACATGATTTTATTACGCCAACCTTATTGCATTTTTTTGGTATCACTACTCAAGAA
GTAGCGTCAGAACCCACCTTTTTTAAGACTACCCATTAA

Upstream 100 bases:

>100_bases
ATCTATTATGCATCTAATATAGACTTCTTAACATCTTCTTAACAGGGTAATTGCTACCTTAGAGAATAACATTTATCTCA
CTCTATTAAGAAGTCTGTGT

Downstream 100 bases:

>100_bases
ACTTTTTTATTCAATTCACACTTTTTGTAGATAGTAGCCATTATGTATAAGATACTTATTATTGAAGACAATCCTGATAT
CGTAGCCAATATCTATGCTT

Product: sulfatase

Products: NA

Alternate protein names: Polymyxin resistance protein pmrC [H]

Number of amino acids: Translated: 572; Mature: 571

Protein sequence:

>572_residues
MSKHLITDLAIAETHNISLAKNNNATSDKNKFYHREVNLTYLIIVVTLYLVATANIGFFKQVLTIYPFADNIGFIVSLTG
LLFGLMWLLFQLLCYRPTAKVVLIAMVLIAAVCGYFTDAYGTIFDTGMLVNSLQTDQAEAMGLIAPSFIVRLLLLGLLPA
FLISKIRIKRLSIVRSIVQKTITLITSIAIIAVCLLPFGDQYASFFRQHKIVRSYTNPITPVYSVIKLGTDYVDELRRPD
TLILHAPDAKRTAAVSSTTKPKLMVFVVGETVRADHIGLNGYKRDTMPLLASQPDIYSYKHATSCGTSTAYSVPCMFSYA
NRSDYDVDTAEYNENVLDTLNKQGVNVIWRDNNSSSKGVADRVTVEDYKTPKLNPNCDIECRDIGMLDGFDTLVKSDTGV
KTAAKPQDTLILLHQMGNHGPAYYQRYPKEFEEYKPVCMTNELSKCDAQSVINGYDNAIRYTDYFLNAVIDTLKPYEQDY
DVVMVYISDHGESLGENNVYLHGLPYGIAPNAQKQVPVIIWSPKNNGINPKSLSNMTNQPVSHDFITPTLLHFFGITTQE
VASEPTFFKTTH

Sequences:

>Translated_572_residues
MSKHLITDLAIAETHNISLAKNNNATSDKNKFYHREVNLTYLIIVVTLYLVATANIGFFKQVLTIYPFADNIGFIVSLTG
LLFGLMWLLFQLLCYRPTAKVVLIAMVLIAAVCGYFTDAYGTIFDTGMLVNSLQTDQAEAMGLIAPSFIVRLLLLGLLPA
FLISKIRIKRLSIVRSIVQKTITLITSIAIIAVCLLPFGDQYASFFRQHKIVRSYTNPITPVYSVIKLGTDYVDELRRPD
TLILHAPDAKRTAAVSSTTKPKLMVFVVGETVRADHIGLNGYKRDTMPLLASQPDIYSYKHATSCGTSTAYSVPCMFSYA
NRSDYDVDTAEYNENVLDTLNKQGVNVIWRDNNSSSKGVADRVTVEDYKTPKLNPNCDIECRDIGMLDGFDTLVKSDTGV
KTAAKPQDTLILLHQMGNHGPAYYQRYPKEFEEYKPVCMTNELSKCDAQSVINGYDNAIRYTDYFLNAVIDTLKPYEQDY
DVVMVYISDHGESLGENNVYLHGLPYGIAPNAQKQVPVIIWSPKNNGINPKSLSNMTNQPVSHDFITPTLLHFFGITTQE
VASEPTFFKTTH
>Mature_571_residues
SKHLITDLAIAETHNISLAKNNNATSDKNKFYHREVNLTYLIIVVTLYLVATANIGFFKQVLTIYPFADNIGFIVSLTGL
LFGLMWLLFQLLCYRPTAKVVLIAMVLIAAVCGYFTDAYGTIFDTGMLVNSLQTDQAEAMGLIAPSFIVRLLLLGLLPAF
LISKIRIKRLSIVRSIVQKTITLITSIAIIAVCLLPFGDQYASFFRQHKIVRSYTNPITPVYSVIKLGTDYVDELRRPDT
LILHAPDAKRTAAVSSTTKPKLMVFVVGETVRADHIGLNGYKRDTMPLLASQPDIYSYKHATSCGTSTAYSVPCMFSYAN
RSDYDVDTAEYNENVLDTLNKQGVNVIWRDNNSSSKGVADRVTVEDYKTPKLNPNCDIECRDIGMLDGFDTLVKSDTGVK
TAAKPQDTLILLHQMGNHGPAYYQRYPKEFEEYKPVCMTNELSKCDAQSVINGYDNAIRYTDYFLNAVIDTLKPYEQDYD
VVMVYISDHGESLGENNVYLHGLPYGIAPNAQKQVPVIIWSPKNNGINPKSLSNMTNQPVSHDFITPTLLHFFGITTQEV
ASEPTFFKTTH

Specific function: Catalyzes the addition of a phosphoethanolamine moiety to the lipid A. The phosphoethanolamine modification is required for resistance to polymyxin [H]

COG id: COG2194

COG function: function code R; Predicted membrane-associated, metal-dependent hydrolase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoethanolamine transferase family. EptA subfamily [H]

Homologues:

Organism=Escherichia coli, GI87082372, Length=522, Percent_Identity=34.2911877394636, Blast_Score=310, Evalue=1e-85,
Organism=Escherichia coli, GI87082286, Length=492, Percent_Identity=25.2032520325203, Blast_Score=150, Evalue=3e-37,
Organism=Escherichia coli, GI87082223, Length=463, Percent_Identity=24.4060475161987, Blast_Score=97, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR017850
- InterPro:   IPR012549
- InterPro:   IPR000917 [H]

Pfam domain/function: PF08019 DUF1705; PF00884 Sulfatase [H]

EC number: NA

Molecular weight: Translated: 63922; Mature: 63791

Theoretical pI: Translated: 7.01; Mature: 7.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKHLITDLAIAETHNISLAKNNNATSDKNKFYHREVNLTYLIIVVTLYLVATANIGFFK
CCCCHHHHHHHEECCCEEEEECCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHCCHHHHH
QVLTIYPFADNIGFIVSLTGLLFGLMWLLFQLLCYRPTAKVVLIAMVLIAAVCGYFTDAY
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
GTIFDTGMLVNSLQTDQAEAMGLIAPSFIVRLLLLGLLPAFLISKIRIKRLSIVRSIVQK
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TITLITSIAIIAVCLLPFGDQYASFFRQHKIVRSYTNPITPVYSVIKLGTDYVDELRRPD
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCHHHHHHHCCCC
TLILHAPDAKRTAAVSSTTKPKLMVFVVGETVRADHIGLNGYKRDTMPLLASQPDIYSYK
EEEEECCCCCHHHHCCCCCCCEEEEEEECCCEECCCCCCCCCCCCCCCHHCCCCCCCCCC
HATSCGTSTAYSVPCMFSYANRSDYDVDTAEYNENVLDTLNKQGVNVIWRDNNSSSKGVA
CCCCCCCCCCEECCEEEEECCCCCCCCCHHHHCCHHHHHHCCCCCEEEEECCCCCCCCCC
DRVTVEDYKTPKLNPNCDIECRDIGMLDGFDTLVKSDTGVKTAAKPQDTLILLHQMGNHG
CEEEECCCCCCCCCCCCCEEEECCCCCCCHHHHHCCCCCCCCCCCCCHHEEEEECCCCCC
PAYYQRYPKEFEEYKPVCMTNELSKCDAQSVINGYDNAIRYTDYFLNAVIDTLKPYEQDY
CHHHHHCCHHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
DVVMVYISDHGESLGENNVYLHGLPYGIAPNAQKQVPVIIWSPKNNGINPKSLSNMTNQP
CEEEEEEECCCCCCCCCCEEEEECCCCCCCCCCCCCCEEEECCCCCCCCHHHHHHHCCCC
VSHDFITPTLLHFFGITTQEVASEPTFFKTTH
CCCCCHHHHHHHHHCCCHHHHCCCCCEEECCC
>Mature Secondary Structure 
SKHLITDLAIAETHNISLAKNNNATSDKNKFYHREVNLTYLIIVVTLYLVATANIGFFK
CCCHHHHHHHEECCCEEEEECCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHCCHHHHH
QVLTIYPFADNIGFIVSLTGLLFGLMWLLFQLLCYRPTAKVVLIAMVLIAAVCGYFTDAY
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
GTIFDTGMLVNSLQTDQAEAMGLIAPSFIVRLLLLGLLPAFLISKIRIKRLSIVRSIVQK
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TITLITSIAIIAVCLLPFGDQYASFFRQHKIVRSYTNPITPVYSVIKLGTDYVDELRRPD
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCHHHHHHHCCCC
TLILHAPDAKRTAAVSSTTKPKLMVFVVGETVRADHIGLNGYKRDTMPLLASQPDIYSYK
EEEEECCCCCHHHHCCCCCCCEEEEEEECCCEECCCCCCCCCCCCCCCHHCCCCCCCCCC
HATSCGTSTAYSVPCMFSYANRSDYDVDTAEYNENVLDTLNKQGVNVIWRDNNSSSKGVA
CCCCCCCCCCEECCEEEEECCCCCCCCCHHHHCCHHHHHHCCCCCEEEEECCCCCCCCCC
DRVTVEDYKTPKLNPNCDIECRDIGMLDGFDTLVKSDTGVKTAAKPQDTLILLHQMGNHG
CEEEECCCCCCCCCCCCCEEEECCCCCCCHHHHHCCCCCCCCCCCCCHHEEEEECCCCCC
PAYYQRYPKEFEEYKPVCMTNELSKCDAQSVINGYDNAIRYTDYFLNAVIDTLKPYEQDY
CHHHHHCCHHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
DVVMVYISDHGESLGENNVYLHGLPYGIAPNAQKQVPVIIWSPKNNGINPKSLSNMTNQP
CEEEEEEECCCCCCCCCCEEEEECCCCCCCCCCCCCCEEEECCCCCCCCHHHHHHHCCCC
VSHDFITPTLLHFFGITTQEVASEPTFFKTTH
CCCCCHHHHHHHHHCCCHHHHCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8391535; 11677609 [H]