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The map label for this gene is cfa [H]

Identifier: 93005560

GI number: 93005560

Start: 859859

End: 861223

Strand: Direct

Name: cfa [H]

Synonym: Pcryo_0730

Alternate gene names: 93005560

Gene position: 859859-861223 (Clockwise)

Preceding gene: 93005559

Following gene: 93005561

Centisome position: 28.1

GC content: 42.34

Gene sequence:

>1365_bases
ATGATGTCAGCAAGACTAGCAAGCAAGGCACCCGTATCAAAATTAGGAAAAATCACTGCTCACATCAATCAAACGCTTAA
CGATAGTGCTATTTTGCAGCCAGTTAGTAATAGCGTGAATTATCTGGCGCGAAAAGCCATATTCCGAGCTTTGAAGCACA
TTCAATTTGGTGGTATTACTATCATTGAGGATTTTGTTGGGCAGGATGCTAAGCTGCATTGTTTTGGCACAGCTGCTTCT
AGCAACATAAGTAGCTCAGCTATCGGTCGTCATACATTGGATGTGACGCTGACCATTCATGATAGCAACGTCTATCGTCA
GTTATTATTTGGTGGTTCTATTGCGCTAGCGGACAGCTATATCAATGGCGAATGGGATACAGATGATTTAACGGGTTTGA
TTCGACTGGCAGCGCGTAATTTGGCAGTACTTAATCAGTTAGAAAATCGTTTTGCGGTTATTAGTAAAACCTTTGAGCGA
GCCAATCATAAGTTACGCAGTAATGATAAATTGGGTTCAAAGTCTAATATTTTAGCGCATTACGACTTAGGTAATGACAT
GTACCAGCGTTTTTTAGACGATACGATGATGTACTCATCAGCAGTCTATAAAAAAGTAGATACGACATTGGCTGAGGCGC
AGCAGCATAAACTCGCTTTGATTTGTGAGCGTTTACAACTGAAACCCGAAGATACTGTCATAGAAATTGGGACTGGTTGG
GGCGGGTTTGCTATTTATGCAGCAACGCATTATGGTTGTCATATAACGACGACCACTATCTCGGATGCGCAATATGAAGA
GGCGCAGCGCCGTGTAGAAGCGGCTGGACTGTCGGATAAAGTCACGTTATTAAAGCAAGACTATCGCGAACTGACAGGAC
AGTATGACAAGTTGGTCAGTATTGAGATGATTGAGGCGGTTGGACATGAGTATTTACCGACGTTTTTTGCTAAATGTAAT
AGCCTGCTGAAGCCAACAGGTTTAATGGTATTACAAGCCATTACCTTCAATGATCAAAATTATCAAGACTATATAAAGTC
GGTCGATTTTATTCAGACGCACATTTTCCCCGGTGGTTGCTTACTATCTAATCAAGAATTGACGACGCAGTTTACTGAGC
AGACCGATATGGTCATCAAACAATTGCATGATTATGGTTTTGACTACGCTTTTACGCTGCGGGATTGGCGTACTGCGTTT
ATGGCACAACGTGAGGAGATAAAAGCACTCGGTTATGATGAAGCGTTTATTCGCTTATGGGAGTTTTATTTCTGCTACTG
CGAAGGGGGGTTTTTGGAACGTACGATTGGAGTCGTGCAAGTCACCGCCGTTAAGCCAAACAACATTGATGCATGGCATT
CATAG

Upstream 100 bases:

>100_bases
AGCAATGACAGTAACTATTATGCCAAAACTTCAAAGGTTCAAAGACTCAAGAATTTAACGTAGAGCTCTTACTATCGAAT
AATTATACAAGGATGGATAA

Downstream 100 bases:

>100_bases
GCTGCATTTTAGTCCCTATTATAAAAGGATCTTACTATGGGTTATATTTATATCTATCTTGCTTCTCTTGTCATATTTTT
GGGCATTGATGCGGTTTGGC

Product: cyclopropane-fatty-acyl-phospholipid synthase

Products: NA

Alternate protein names: CFA synthase; Cyclopropane fatty acid synthase [H]

Number of amino acids: Translated: 454; Mature: 454

Protein sequence:

>454_residues
MMSARLASKAPVSKLGKITAHINQTLNDSAILQPVSNSVNYLARKAIFRALKHIQFGGITIIEDFVGQDAKLHCFGTAAS
SNISSSAIGRHTLDVTLTIHDSNVYRQLLFGGSIALADSYINGEWDTDDLTGLIRLAARNLAVLNQLENRFAVISKTFER
ANHKLRSNDKLGSKSNILAHYDLGNDMYQRFLDDTMMYSSAVYKKVDTTLAEAQQHKLALICERLQLKPEDTVIEIGTGW
GGFAIYAATHYGCHITTTTISDAQYEEAQRRVEAAGLSDKVTLLKQDYRELTGQYDKLVSIEMIEAVGHEYLPTFFAKCN
SLLKPTGLMVLQAITFNDQNYQDYIKSVDFIQTHIFPGGCLLSNQELTTQFTEQTDMVIKQLHDYGFDYAFTLRDWRTAF
MAQREEIKALGYDEAFIRLWEFYFCYCEGGFLERTIGVVQVTAVKPNNIDAWHS

Sequences:

>Translated_454_residues
MMSARLASKAPVSKLGKITAHINQTLNDSAILQPVSNSVNYLARKAIFRALKHIQFGGITIIEDFVGQDAKLHCFGTAAS
SNISSSAIGRHTLDVTLTIHDSNVYRQLLFGGSIALADSYINGEWDTDDLTGLIRLAARNLAVLNQLENRFAVISKTFER
ANHKLRSNDKLGSKSNILAHYDLGNDMYQRFLDDTMMYSSAVYKKVDTTLAEAQQHKLALICERLQLKPEDTVIEIGTGW
GGFAIYAATHYGCHITTTTISDAQYEEAQRRVEAAGLSDKVTLLKQDYRELTGQYDKLVSIEMIEAVGHEYLPTFFAKCN
SLLKPTGLMVLQAITFNDQNYQDYIKSVDFIQTHIFPGGCLLSNQELTTQFTEQTDMVIKQLHDYGFDYAFTLRDWRTAF
MAQREEIKALGYDEAFIRLWEFYFCYCEGGFLERTIGVVQVTAVKPNNIDAWHS
>Mature_454_residues
MMSARLASKAPVSKLGKITAHINQTLNDSAILQPVSNSVNYLARKAIFRALKHIQFGGITIIEDFVGQDAKLHCFGTAAS
SNISSSAIGRHTLDVTLTIHDSNVYRQLLFGGSIALADSYINGEWDTDDLTGLIRLAARNLAVLNQLENRFAVISKTFER
ANHKLRSNDKLGSKSNILAHYDLGNDMYQRFLDDTMMYSSAVYKKVDTTLAEAQQHKLALICERLQLKPEDTVIEIGTGW
GGFAIYAATHYGCHITTTTISDAQYEEAQRRVEAAGLSDKVTLLKQDYRELTGQYDKLVSIEMIEAVGHEYLPTFFAKCN
SLLKPTGLMVLQAITFNDQNYQDYIKSVDFIQTHIFPGGCLLSNQELTTQFTEQTDMVIKQLHDYGFDYAFTLRDWRTAF
MAQREEIKALGYDEAFIRLWEFYFCYCEGGFLERTIGVVQVTAVKPNNIDAWHS

Specific function: Transfers a methylene group from S-adenosyl-L-methionine to the cis double bond of an unsaturated fatty acid chain resulting in the replacement of the double bond with a methylene bridge [H]

COG id: COG2230

COG function: function code M; Cyclopropane fatty acid synthase and related methyltransferases

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CFA/CMAS family [H]

Homologues:

Organism=Escherichia coli, GI1787951, Length=349, Percent_Identity=37.5358166189112, Blast_Score=206, Evalue=3e-54,
Organism=Caenorhabditis elegans, GI133904226, Length=405, Percent_Identity=36.7901234567901, Blast_Score=223, Evalue=2e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003333 [H]

Pfam domain/function: PF02353 CMAS [H]

EC number: =2.1.1.79 [H]

Molecular weight: Translated: 51111; Mature: 51111

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMSARLASKAPVSKLGKITAHINQTLNDSAILQPVSNSVNYLARKAIFRALKHIQFGGIT
CCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
IIEDFVGQDAKLHCFGTAASSNISSSAIGRHTLDVTLTIHDSNVYRQLLFGGSIALADSY
EEHHHCCCCCEEEEEEECCCCCCCHHHCCCEEEEEEEEEECCHHHHHHHHCCCEEEEECC
INGEWDTDDLTGLIRLAARNLAVLNQLENRFAVISKTFERANHKLRSNDKLGSKSNILAH
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCCCCEEEE
YDLGNDMYQRFLDDTMMYSSAVYKKVDTTLAEAQQHKLALICERLQLKPEDTVIEIGTGW
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCC
GGFAIYAATHYGCHITTTTISDAQYEEAQRRVEAAGLSDKVTLLKQDYRELTGQYDKLVS
CCEEEEEEEECCEEEEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHH
IEMIEAVGHEYLPTFFAKCNSLLKPTGLMVLQAITFNDQNYQDYIKSVDFIQTHIFPGGC
HHHHHHHCHHHHHHHHHHHHHHCCCCHHHEEEEEECCCCCHHHHHHHHHHHHHHCCCCCE
LLSNQELTTQFTEQTDMVIKQLHDYGFDYAFTLRDWRTAFMAQREEIKALGYDEAFIRLW
EECCCHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHCCCCHHHHHHH
EFYFCYCEGGFLERTIGVVQVTAVKPNNIDAWHS
HHHHHEECCCHHHHEEEEEEEEEECCCCCCCCCC
>Mature Secondary Structure
MMSARLASKAPVSKLGKITAHINQTLNDSAILQPVSNSVNYLARKAIFRALKHIQFGGIT
CCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
IIEDFVGQDAKLHCFGTAASSNISSSAIGRHTLDVTLTIHDSNVYRQLLFGGSIALADSY
EEHHHCCCCCEEEEEEECCCCCCCHHHCCCEEEEEEEEEECCHHHHHHHHCCCEEEEECC
INGEWDTDDLTGLIRLAARNLAVLNQLENRFAVISKTFERANHKLRSNDKLGSKSNILAH
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCCCCEEEE
YDLGNDMYQRFLDDTMMYSSAVYKKVDTTLAEAQQHKLALICERLQLKPEDTVIEIGTGW
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCC
GGFAIYAATHYGCHITTTTISDAQYEEAQRRVEAAGLSDKVTLLKQDYRELTGQYDKLVS
CCEEEEEEEECCEEEEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHH
IEMIEAVGHEYLPTFFAKCNSLLKPTGLMVLQAITFNDQNYQDYIKSVDFIQTHIFPGGC
HHHHHHHCHHHHHHHHHHHHHHCCCCHHHEEEEEECCCCCHHHHHHHHHHHHHHCCCCCE
LLSNQELTTQFTEQTDMVIKQLHDYGFDYAFTLRDWRTAFMAQREEIKALGYDEAFIRLW
EECCCHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHCCCCHHHHHHH
EFYFCYCEGGFLERTIGVVQVTAVKPNNIDAWHS
HHHHHEECCCHHHHEEEEEEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]