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The map label for this gene is pitB [C]

Identifier: 93005521

GI number: 93005521

Start: 820116

End: 821723

Strand: Direct

Name: pitB [C]

Synonym: Pcryo_0691

Alternate gene names: 93005521

Gene position: 820116-821723 (Clockwise)

Preceding gene: 93005520

Following gene: 93005523

Centisome position: 26.8

GC content: 44.47

Gene sequence:

>1608_bases
ATGGGTATTAGCAGCAGCTCTACAGAACCAACAAAATCAGTCGGCTCTATGAGAATCAATCTATTTTTTGCCATTTTAAT
GATTGGAATGACCGCCTACTTTTTGTGGTGGGGTCTAGATTACACCATGCACCAGCAGACGACGCTATTTATTGTTGCCA
CTGCTTTTGGTGTTTTTATGGCATTCAATATCGGCGGTAATGATGTCGCCAACTCTTTTGGTACCTCAGTCGGTGCAGGG
ACCTTAACCATCCCGCAAGCGCTAGGTATCGCGGCGATATTTGAAGTATCAGGCGCCGTGCTGGCAGGCGGTGAAGTAAC
CGATACGATTCGAAGCGGCATTGTCGATTTAGATGGTCTTTCAGTAACGCCCAACCAGTTCATTTATGTCATGCTTTCAG
CGCTCATCGCTGCTGCATTTTGGCTGTTGTTTGCCACCAAAAAAGGTCTGCCGGTTTCGACCACACACGCGATTATTGGC
GGCGTAGTTGGTAGCTCTATTGTTTTGGGTATCACCTTAGGTGGTACTGAAATGGCATTGTCGACAGTGAATTGGGGCAA
GATTGGCACGATCGCCATCTCTTGGGTTTTGTCACCGCTACTAGGCGGCGTTTTGTCTTATCTGCTATATGGACAGATTA
AGAAAAACATCATTGAATATAATGATAAAACAGAGGCGCATATTGCTACTCTGAAAGACAGTAAAAAGACGTTAAAGCTG
ACGCACAAAGAGTACTTGGATGGTTTAACCGAGTCTGAGCAGTTGTCTTATACGTCAGCGATGTTGCGTGACCAAGAAAT
CTATAAAGATGATGATTGCGTCGTTGAAGATTTAGAGACGAACTATTACAAAGAACTTTATGAGATTGAAAACGAGCGCA
GCAACCTTGATACCTTAAAAGCACTCAAGCAATGGGTGCCTATTATTGCGGCTGCAGGCGGCGCAGTCATGGCCTCTTTG
GTCATCTTCAAAGGTCTTAAAAACGTCAATAATGGTATGACGACGTTGCAAGGCTTTTTAATTATGGGGATGATCGCAGC
ATTAGTATGGCTTGCGACCTATATTTATACTAAGAGTATCCGCGGTAAGCACAAAGAAGATTTGACCAAAGCCACGTTTA
TTATGTTTAGCTGGATGCAAGTCTTTACTGCATCAGCGTTTGCCTTTAGTCATGGTTCTAACGATATTGCCAACGCTGTT
GGTCCATTTGCAGCGATTATGGATGTTATCCGTACCAATAGTATCTCAACAGAAGCAGCAGTACCACCTGCTGTGATGTT
AACTTTTGGTGTGGCATTGATTGTCGGTCTTTGGTTCATCGGTAAAGAAGTGATTCAGACTGTCGGTACCAATTTGGCAA
AAATGCATCCTGCTTCTGGTTTCTCAGCTGAGTTGGCTGCGGCTGCGGTTGTGATGGGTGCGTCGACGATGGGTCTACCA
GTATCGAGTACGCATACCTTGGTCGGTGCTGTACTTGGCATTGGTATCGTCAATAGAGATACCAACTGGGCACTGATGAA
ACCAATTGGTTTGGCATGGATTATTACCCTTCCTGCAGCAGCAAGTATGTCTGCAATCAGTTATATAGTGCTGATAAATA
TCTTTTAA

Upstream 100 bases:

>100_bases
GTTATTGTATTTTTGGGTGGTAATAATCACATCATTAATAGCCGCGTAATGTCATCATTTGTCAATAATTTCAATCAGCT
TACGCAGGACGTATCAACGT

Downstream 100 bases:

>100_bases
AAGTTGAAATCAAAGTCATTTAATTTTTGATTAATACAAATAGAAAACGCTTATCGATATCATATCGGTAAGCGTTTTTT
TATAGAAAAATATAGGTGTC

Product: phosphate transporter

Products: Proton [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 535; Mature: 534

Protein sequence:

>535_residues
MGISSSSTEPTKSVGSMRINLFFAILMIGMTAYFLWWGLDYTMHQQTTLFIVATAFGVFMAFNIGGNDVANSFGTSVGAG
TLTIPQALGIAAIFEVSGAVLAGGEVTDTIRSGIVDLDGLSVTPNQFIYVMLSALIAAAFWLLFATKKGLPVSTTHAIIG
GVVGSSIVLGITLGGTEMALSTVNWGKIGTIAISWVLSPLLGGVLSYLLYGQIKKNIIEYNDKTEAHIATLKDSKKTLKL
THKEYLDGLTESEQLSYTSAMLRDQEIYKDDDCVVEDLETNYYKELYEIENERSNLDTLKALKQWVPIIAAAGGAVMASL
VIFKGLKNVNNGMTTLQGFLIMGMIAALVWLATYIYTKSIRGKHKEDLTKATFIMFSWMQVFTASAFAFSHGSNDIANAV
GPFAAIMDVIRTNSISTEAAVPPAVMLTFGVALIVGLWFIGKEVIQTVGTNLAKMHPASGFSAELAAAAVVMGASTMGLP
VSSTHTLVGAVLGIGIVNRDTNWALMKPIGLAWIITLPAAASMSAISYIVLINIF

Sequences:

>Translated_535_residues
MGISSSSTEPTKSVGSMRINLFFAILMIGMTAYFLWWGLDYTMHQQTTLFIVATAFGVFMAFNIGGNDVANSFGTSVGAG
TLTIPQALGIAAIFEVSGAVLAGGEVTDTIRSGIVDLDGLSVTPNQFIYVMLSALIAAAFWLLFATKKGLPVSTTHAIIG
GVVGSSIVLGITLGGTEMALSTVNWGKIGTIAISWVLSPLLGGVLSYLLYGQIKKNIIEYNDKTEAHIATLKDSKKTLKL
THKEYLDGLTESEQLSYTSAMLRDQEIYKDDDCVVEDLETNYYKELYEIENERSNLDTLKALKQWVPIIAAAGGAVMASL
VIFKGLKNVNNGMTTLQGFLIMGMIAALVWLATYIYTKSIRGKHKEDLTKATFIMFSWMQVFTASAFAFSHGSNDIANAV
GPFAAIMDVIRTNSISTEAAVPPAVMLTFGVALIVGLWFIGKEVIQTVGTNLAKMHPASGFSAELAAAAVVMGASTMGLP
VSSTHTLVGAVLGIGIVNRDTNWALMKPIGLAWIITLPAAASMSAISYIVLINIF
>Mature_534_residues
GISSSSTEPTKSVGSMRINLFFAILMIGMTAYFLWWGLDYTMHQQTTLFIVATAFGVFMAFNIGGNDVANSFGTSVGAGT
LTIPQALGIAAIFEVSGAVLAGGEVTDTIRSGIVDLDGLSVTPNQFIYVMLSALIAAAFWLLFATKKGLPVSTTHAIIGG
VVGSSIVLGITLGGTEMALSTVNWGKIGTIAISWVLSPLLGGVLSYLLYGQIKKNIIEYNDKTEAHIATLKDSKKTLKLT
HKEYLDGLTESEQLSYTSAMLRDQEIYKDDDCVVEDLETNYYKELYEIENERSNLDTLKALKQWVPIIAAAGGAVMASLV
IFKGLKNVNNGMTTLQGFLIMGMIAALVWLATYIYTKSIRGKHKEDLTKATFIMFSWMQVFTASAFAFSHGSNDIANAVG
PFAAIMDVIRTNSISTEAAVPPAVMLTFGVALIVGLWFIGKEVIQTVGTNLAKMHPASGFSAELAAAAVVMGASTMGLPV
SSTHTLVGAVLGIGIVNRDTNWALMKPIGLAWIITLPAAASMSAISYIVLINIF

Specific function: Potential transporter for phosphate [H]

COG id: COG0306

COG function: function code P; Phosphate/sulphate permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family [H]

Homologues:

Organism=Homo sapiens, GI31543630, Length=165, Percent_Identity=40.6060606060606, Blast_Score=132, Evalue=6e-31,
Organism=Homo sapiens, GI5803173, Length=154, Percent_Identity=40.9090909090909, Blast_Score=128, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI71981576, Length=487, Percent_Identity=32.2381930184805, Blast_Score=216, Evalue=3e-56,
Organism=Caenorhabditis elegans, GI17539280, Length=536, Percent_Identity=29.6641791044776, Blast_Score=211, Evalue=9e-55,
Organism=Caenorhabditis elegans, GI25146401, Length=172, Percent_Identity=41.2790697674419, Blast_Score=138, Evalue=8e-33,
Organism=Caenorhabditis elegans, GI32566716, Length=160, Percent_Identity=41.875, Blast_Score=125, Evalue=6e-29,
Organism=Caenorhabditis elegans, GI17536725, Length=165, Percent_Identity=33.9393939393939, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17557328, Length=226, Percent_Identity=28.7610619469027, Blast_Score=97, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6319773, Length=162, Percent_Identity=35.1851851851852, Blast_Score=115, Evalue=1e-26,
Organism=Drosophila melanogaster, GI21356511, Length=172, Percent_Identity=41.8604651162791, Blast_Score=125, Evalue=6e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001204 [H]

Pfam domain/function: PF01384 PHO4 [H]

EC number: NA

Molecular weight: Translated: 57121; Mature: 56990

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGISSSSTEPTKSVGSMRINLFFAILMIGMTAYFLWWGLDYTMHQQTTLFIVATAFGVFM
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCHHEEHHHHHHHHHH
AFNIGGNDVANSFGTSVGAGTLTIPQALGIAAIFEVSGAVLAGGEVTDTIRSGIVDLDGL
EEECCCHHHHHHHCCCCCCCCEEHHHHHHHHHHHHCCCCEEECCCHHHHHHHCCEECCCC
SVTPNQFIYVMLSALIAAAFWLLFATKKGLPVSTTHAIIGGVVGSSIVLGITLGGTEMAL
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCEEEEEEECCHHHHE
STVNWGKIGTIAISWVLSPLLGGVLSYLLYGQIKKNIIEYNDKTEAHIATLKDSKKTLKL
EECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHCCHHHHHH
THKEYLDGLTESEQLSYTSAMLRDQEIYKDDDCVVEDLETNYYKELYEIENERSNLDTLK
HHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHH
ALKQWVPIIAAAGGAVMASLVIFKGLKNVNNGMTTLQGFLIMGMIAALVWLATYIYTKSI
HHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RGKHKEDLTKATFIMFSWMQVFTASAFAFSHGSNDIANAVGPFAAIMDVIRTNSISTEAA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCC
VPPAVMLTFGVALIVGLWFIGKEVIQTVGTNLAKMHPASGFSAELAAAAVVMGASTMGLP
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCHHHHHHHHHHHCCCCCCCC
VSSTHTLVGAVLGIGIVNRDTNWALMKPIGLAWIITLPAAASMSAISYIVLINIF
CCCHHHHHHHHHHHHEECCCCCEEEECCCCEEEEEECCCHHHHHHHEEEEEEEEC
>Mature Secondary Structure 
GISSSSTEPTKSVGSMRINLFFAILMIGMTAYFLWWGLDYTMHQQTTLFIVATAFGVFM
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCHHEEHHHHHHHHHH
AFNIGGNDVANSFGTSVGAGTLTIPQALGIAAIFEVSGAVLAGGEVTDTIRSGIVDLDGL
EEECCCHHHHHHHCCCCCCCCEEHHHHHHHHHHHHCCCCEEECCCHHHHHHHCCEECCCC
SVTPNQFIYVMLSALIAAAFWLLFATKKGLPVSTTHAIIGGVVGSSIVLGITLGGTEMAL
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCEEEEEEECCHHHHE
STVNWGKIGTIAISWVLSPLLGGVLSYLLYGQIKKNIIEYNDKTEAHIATLKDSKKTLKL
EECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHCCHHHHHH
THKEYLDGLTESEQLSYTSAMLRDQEIYKDDDCVVEDLETNYYKELYEIENERSNLDTLK
HHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHH
ALKQWVPIIAAAGGAVMASLVIFKGLKNVNNGMTTLQGFLIMGMIAALVWLATYIYTKSI
HHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RGKHKEDLTKATFIMFSWMQVFTASAFAFSHGSNDIANAVGPFAAIMDVIRTNSISTEAA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCC
VPPAVMLTFGVALIVGLWFIGKEVIQTVGTNLAKMHPASGFSAELAAAAVVMGASTMGLP
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCHHHHHHHHHHHCCCCCCCC
VSSTHTLVGAVLGIGIVNRDTNWALMKPIGLAWIITLPAAASMSAISYIVLINIF
CCCHHHHHHHHHHHHEECCCCCEEEECCCCEEEEEECCCHHHHHHHEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; phosphate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + phosphate [Periplasm] = Proton [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]