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The map label for this gene is pntB [H]

Identifier: 93005375

GI number: 93005375

Start: 643346

End: 644746

Strand: Direct

Name: pntB [H]

Synonym: Pcryo_0545

Alternate gene names: 93005375

Gene position: 643346-644746 (Clockwise)

Preceding gene: 93005374

Following gene: 93005376

Centisome position: 21.03

GC content: 46.97

Gene sequence:

>1401_bases
ATGAGTGACTTATCAAATATGATTGCGGCTAATGCGGACTGGTTTTATCTGGTCGGTGCCATTCTTTTTGTTTTGACGTT
ACGTGGTTTATCTAGCCCAAAAACGGCGATTCGTGGTAATCGTTTGGGTATGGTTGCCATGGCGATTGCCGTTGTAACAA
CGTTCTTCCTAGCAGAAGGTCCAGTGCTTTGGTTAATCATCGGTGCGATGGTATTGGGTGCTATCGTCGGTATGTGGAAA
GCAAAGACGGTCGCCATGACCCAAATGCCAGAAACCGTGGCTTTGATGCACTCGTTTGTTGGTTTGGCAGCGGTGGCGAT
TGCATTAGCAACGGTACTGCATACTGAACAACAGCATGGCGCGGTTGCTCGTGTTGAATTGTTTATTGGTTGTTTCATCG
GTGCGATTACTTTCTCAGCATCTGTCTTTGCCTTTGGTAAATTGGCAGCTAAGAGCTGGGCTAAGACCTTAGTTGGTAGT
TGGGTAAAACCAGTACAAGCAGCACTTTTCATTGCGATGATTGCGTTCGGTGGTGTGTACTTTGTCACTGATTCATTACC
CGCCTTCTATGCGATGGCAGTGATTGCAGTGATTTTTGGTTGGATGTGGATTGCGCCAATTGGTGGTGGCGATATGCCAG
TGGTCGTGTCGCTATTGAACTCATTTTCAGGGTGGGCAGCAGCGGGTATTGGTTTCACCCTTGGCAACTCGATGTTGATT
ATCGCAGGTTCGCTAGTCGGTTCGTCAGGTGCGATTCTATCTTATATCATGTGTAAAGCCATGAACCGCTCATTACTCAA
CGTATTATTTGGTGGTATGGGTACATCAGCGGTCGCAGCGGGCGGCGATGATGGCGCGCCAAAGAATTACAAAGCAGGCT
CAGCAGAAGATGCTGGCTTCCTTATGGCAAATGCTAGTAGTGTGGTGATTGTCCCAGGTTACGGGATGGCGCAAGGTCGT
GCACAGAATGCGGTCAAAGAATTGTATGAGCTGTTAAAAGAAGAAGGCGTTAATGTTCGTTTTGCCATTCATCCAGTCGC
GGGTCGTATGCCAGGACATATGAACGTCCTATTGGCAGAAGCCGATGTGCCCTATGATGATATTTTAGAGATGGATGAGA
TTAACTCGGACTTCGCCAGTACGGATGTGGTATTAGTTATCGGTGCCAATGACGTGGTAAACCCATCTGCGAAAGATGAT
CCGACATCACCAATCTTTGGTATGCCAATTCTAGAAGTGACCAAAGCCCAAACAGTGATGGTCATTAAGCGTTCGATGAG
TACAGGTTATGCCGGTCTAGATAACAGCTTGTTCTATATGGACAAAACCATGATGATCTTTGGCGATGCCAAGAAAATGG
TTGAAGAGATGGTACGTAGTATCAACGGCGCTGGTCATTAA

Upstream 100 bases:

>100_bases
TCAATATTTTTGGTGGTTTCGCCGTGACTGAGCGTATGCTGTCGATGTTTAAGCCAAAAGTAAAAAAAGCAGTAGTAACA
ACGGATACTGGGGGTGACGC

Downstream 100 bases:

>100_bases
TCTTTAAGATAGGTTTTACACATCATTTTTTAAACAGAAGCTTTCACATAAAAAAGTCACTGAGGTGGCTTTTTTTGCGT
TAGAGTATTGCCCATCTAAA

Product: NAD(P) transhydrogenase subunit beta

Products: NA

Alternate protein names: Nicotinamide nucleotide transhydrogenase subunit beta; Proton-translocating transhydrogenase NADP(H)-binding component; Pyridine nucleotide transhydrogenase subunit beta; dIII [H]

Number of amino acids: Translated: 466; Mature: 465

Protein sequence:

>466_residues
MSDLSNMIAANADWFYLVGAILFVLTLRGLSSPKTAIRGNRLGMVAMAIAVVTTFFLAEGPVLWLIIGAMVLGAIVGMWK
AKTVAMTQMPETVALMHSFVGLAAVAIALATVLHTEQQHGAVARVELFIGCFIGAITFSASVFAFGKLAAKSWAKTLVGS
WVKPVQAALFIAMIAFGGVYFVTDSLPAFYAMAVIAVIFGWMWIAPIGGGDMPVVVSLLNSFSGWAAAGIGFTLGNSMLI
IAGSLVGSSGAILSYIMCKAMNRSLLNVLFGGMGTSAVAAGGDDGAPKNYKAGSAEDAGFLMANASSVVIVPGYGMAQGR
AQNAVKELYELLKEEGVNVRFAIHPVAGRMPGHMNVLLAEADVPYDDILEMDEINSDFASTDVVLVIGANDVVNPSAKDD
PTSPIFGMPILEVTKAQTVMVIKRSMSTGYAGLDNSLFYMDKTMMIFGDAKKMVEEMVRSINGAGH

Sequences:

>Translated_466_residues
MSDLSNMIAANADWFYLVGAILFVLTLRGLSSPKTAIRGNRLGMVAMAIAVVTTFFLAEGPVLWLIIGAMVLGAIVGMWK
AKTVAMTQMPETVALMHSFVGLAAVAIALATVLHTEQQHGAVARVELFIGCFIGAITFSASVFAFGKLAAKSWAKTLVGS
WVKPVQAALFIAMIAFGGVYFVTDSLPAFYAMAVIAVIFGWMWIAPIGGGDMPVVVSLLNSFSGWAAAGIGFTLGNSMLI
IAGSLVGSSGAILSYIMCKAMNRSLLNVLFGGMGTSAVAAGGDDGAPKNYKAGSAEDAGFLMANASSVVIVPGYGMAQGR
AQNAVKELYELLKEEGVNVRFAIHPVAGRMPGHMNVLLAEADVPYDDILEMDEINSDFASTDVVLVIGANDVVNPSAKDD
PTSPIFGMPILEVTKAQTVMVIKRSMSTGYAGLDNSLFYMDKTMMIFGDAKKMVEEMVRSINGAGH
>Mature_465_residues
SDLSNMIAANADWFYLVGAILFVLTLRGLSSPKTAIRGNRLGMVAMAIAVVTTFFLAEGPVLWLIIGAMVLGAIVGMWKA
KTVAMTQMPETVALMHSFVGLAAVAIALATVLHTEQQHGAVARVELFIGCFIGAITFSASVFAFGKLAAKSWAKTLVGSW
VKPVQAALFIAMIAFGGVYFVTDSLPAFYAMAVIAVIFGWMWIAPIGGGDMPVVVSLLNSFSGWAAAGIGFTLGNSMLII
AGSLVGSSGAILSYIMCKAMNRSLLNVLFGGMGTSAVAAGGDDGAPKNYKAGSAEDAGFLMANASSVVIVPGYGMAQGRA
QNAVKELYELLKEEGVNVRFAIHPVAGRMPGHMNVLLAEADVPYDDILEMDEINSDFASTDVVLVIGANDVVNPSAKDDP
TSPIFGMPILEVTKAQTVMVIKRSMSTGYAGLDNSLFYMDKTMMIFGDAKKMVEEMVRSINGAGH

Specific function: The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane [H]

COG id: COG1282

COG function: function code C; NAD/NADP transhydrogenase beta subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNT beta subunit family [H]

Homologues:

Organism=Homo sapiens, GI122939155, Length=459, Percent_Identity=45.0980392156863, Blast_Score=359, Evalue=3e-99,
Organism=Homo sapiens, GI122939153, Length=459, Percent_Identity=45.0980392156863, Blast_Score=359, Evalue=3e-99,
Organism=Escherichia coli, GI1787886, Length=455, Percent_Identity=53.1868131868132, Blast_Score=459, Evalue=1e-130,
Organism=Caenorhabditis elegans, GI17550456, Length=464, Percent_Identity=42.2413793103448, Blast_Score=340, Evalue=7e-94,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012136
- InterPro:   IPR004003 [H]

Pfam domain/function: PF02233 PNTB [H]

EC number: =1.6.1.2 [H]

Molecular weight: Translated: 49006; Mature: 48875

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
6.4 %Met     (Translated Protein)
6.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
6.2 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHCCCCCCCCCCEEEECCEEEEECCHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SDLSNMIAANADWFYLVGAILFVLTLRGLSSPKTAIRGNRLGMVAMAIAVVTTFFLAEG
CCHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHCC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HHHHHHCCCCCCCCCCEEEECCEEEEECCHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8075801 [H]