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The map label for this gene is ilvI [H]

Identifier: 93005352

GI number: 93005352

Start: 618278

End: 619990

Strand: Direct

Name: ilvI [H]

Synonym: Pcryo_0522

Alternate gene names: 93005352

Gene position: 618278-619990 (Clockwise)

Preceding gene: 93005350

Following gene: 93005353

Centisome position: 20.21

GC content: 44.42

Gene sequence:

>1713_bases
ATGCTATCTGGTGCTGAGATGTTGGTGCAAGCACTAACGGATGAGGGCGTCAAATACATTTTTGGTTATCCAGGCGGTGC
GGTCCTTCATATCTATGACGCCTTATTTCAACAAGACAATGTTGAGCATATTCTGGTACGTCATGAACAAGCCGCAGGTC
ACATGGCAGATGCTTATTCAAGAGTAACTGGCAACACGGGTGTGGTGCTAGCGACTTCAGGTCCTGGTGCGACCAATACA
GTGACAGCTATTGCCACTGCTTTTATGGATTCTGTACCGATGGTGATCATCGCAGGTCAGGTGCCTAGTAGTCTGATCGG
TGAAGATGCTTTCCAAGAAACCGATATGATTGGTGTGTCACGCCCAATCGTGAAGCATAGCTTTCAAGTGCGCCATGCTA
GCGAAATCCCAATGATTGTCAAAAAAGCCTTTTATATTGCCCAGTCTGGACGTCCGGGTCCTGTTGTCATCGATGTGCCA
AAAGACATGACTGCGCCTAGCGAAAAATTTGCTTATGAATATCCAAAAGAGGTATTTATACGCTCATATCAGCCGTCATT
AAAAGGTCATAGTGGTCAAATCAAAAAGGCGGTTGAAACCTTACTTGCGGCTAAGCGCCCGATTATCTACTCTGGTGGCG
GTGTAGTTTTAGGTAATGCCTATAAAGAATTAACGGCGCTTGCCCATCGTTTAAACCTACCAGTCACCAATACCTTAATG
GGTTTGGGTGCTTTTCCTGGCTCTGATGAGCAGTTCTTGGGTATGCTTGGTATGCATGGCACTTATGAAGCCAATATGAC
CATGCATCATTCTGACGTTATATTGGCAGTTGGGGCACGCTTTGATGATCGCGTGACCAATAACGTCAAAAAATTCTGTC
CAAATGCGACGATTATTCATATTGATATTGACCCAACGTCTATCTCAAAAACGATCTTTGCTCATATTCCTATCGTTGGC
GATGTGAAATCAGTGCTTACTGACATGATAGACATTATTGGCGAAGATAAAGAGTTAGACCAACCGGCGTTATTGGATTG
GTGGTCACAGATTAATGAGTGGCGCAAGCGTCATGGTTTGCGCTATGACACCAGTACCGATAATGGTATTAAGCCACAAG
CGGTCGTTCAAGCTTTAGATAAAGTGACTAATAGTAATGCGATTATCACGAGTGACGTTGGTCAACATCAGATGTTTGCC
GCGCTTTACTATACCTATAATGAGCCACGTCAATGGCTGAACTCAGGTGGTCTTGGTACTATGGGCGTTGGCTTGCCATA
TGCGATGGCAGCCAAACTTGCTAATCCTGAGCGTGATGTGGTTTGTATTACCGGCGAAGGCTCTATTCAGATGAATATCC
AAGAACTGTCTACTTGTTTGCAGTATGATTTGCCAGTTAAAATCCTAAACTTGAATAATGCACAGTTGGGTATGGTAAAA
CAGTGGCAAGACATGCTATATGAAGGTCGTCACGCACATTCTTACATGAATTCTTTACCAGACTTTGTCAAGCTTGCTGA
AAGCTATGGTCACGTCGGTATCAAGATTACTGACCCTGCGACCATGGAAGCACAATTGGCTGAAGCCATGGCAATAAAAG
ACAGGTTGGTATTTATCGATGTTTACGTCGATCGTAATGAGCATGTATATCCGATGCAAATCGCTGGGCAAACGATGCGT
GATATGTGGTTAACAAAAGGGGAGCGTACCTAA

Upstream 100 bases:

>100_bases
GCAAAGTCCGAATATGACTGGACATACCCAAACGGGTCATGCTGCCAAGAATTTCCCTGAAAAGCAGCAACGCCTTGCCG
AAGGAAGCAATGAGCCTGTA

Downstream 100 bases:

>100_bases
TGCAACAACAACATCTGATTTCGGTATTGATGGAAAACGAAGCGGGTTCGTTGTCGCGTTTAGTCGGCTTGTTCTCACAA
CGTGGCTACAATATCGAAAC

Product: acetolactate synthase 3 catalytic subunit

Products: NA

Alternate protein names: AHAS-III; ALS-III; Acetohydroxy-acid synthase III large subunit [H]

Number of amino acids: Translated: 570; Mature: 570

Protein sequence:

>570_residues
MLSGAEMLVQALTDEGVKYIFGYPGGAVLHIYDALFQQDNVEHILVRHEQAAGHMADAYSRVTGNTGVVLATSGPGATNT
VTAIATAFMDSVPMVIIAGQVPSSLIGEDAFQETDMIGVSRPIVKHSFQVRHASEIPMIVKKAFYIAQSGRPGPVVIDVP
KDMTAPSEKFAYEYPKEVFIRSYQPSLKGHSGQIKKAVETLLAAKRPIIYSGGGVVLGNAYKELTALAHRLNLPVTNTLM
GLGAFPGSDEQFLGMLGMHGTYEANMTMHHSDVILAVGARFDDRVTNNVKKFCPNATIIHIDIDPTSISKTIFAHIPIVG
DVKSVLTDMIDIIGEDKELDQPALLDWWSQINEWRKRHGLRYDTSTDNGIKPQAVVQALDKVTNSNAIITSDVGQHQMFA
ALYYTYNEPRQWLNSGGLGTMGVGLPYAMAAKLANPERDVVCITGEGSIQMNIQELSTCLQYDLPVKILNLNNAQLGMVK
QWQDMLYEGRHAHSYMNSLPDFVKLAESYGHVGIKITDPATMEAQLAEAMAIKDRLVFIDVYVDRNEHVYPMQIAGQTMR
DMWLTKGERT

Sequences:

>Translated_570_residues
MLSGAEMLVQALTDEGVKYIFGYPGGAVLHIYDALFQQDNVEHILVRHEQAAGHMADAYSRVTGNTGVVLATSGPGATNT
VTAIATAFMDSVPMVIIAGQVPSSLIGEDAFQETDMIGVSRPIVKHSFQVRHASEIPMIVKKAFYIAQSGRPGPVVIDVP
KDMTAPSEKFAYEYPKEVFIRSYQPSLKGHSGQIKKAVETLLAAKRPIIYSGGGVVLGNAYKELTALAHRLNLPVTNTLM
GLGAFPGSDEQFLGMLGMHGTYEANMTMHHSDVILAVGARFDDRVTNNVKKFCPNATIIHIDIDPTSISKTIFAHIPIVG
DVKSVLTDMIDIIGEDKELDQPALLDWWSQINEWRKRHGLRYDTSTDNGIKPQAVVQALDKVTNSNAIITSDVGQHQMFA
ALYYTYNEPRQWLNSGGLGTMGVGLPYAMAAKLANPERDVVCITGEGSIQMNIQELSTCLQYDLPVKILNLNNAQLGMVK
QWQDMLYEGRHAHSYMNSLPDFVKLAESYGHVGIKITDPATMEAQLAEAMAIKDRLVFIDVYVDRNEHVYPMQIAGQTMR
DMWLTKGERT
>Mature_570_residues
MLSGAEMLVQALTDEGVKYIFGYPGGAVLHIYDALFQQDNVEHILVRHEQAAGHMADAYSRVTGNTGVVLATSGPGATNT
VTAIATAFMDSVPMVIIAGQVPSSLIGEDAFQETDMIGVSRPIVKHSFQVRHASEIPMIVKKAFYIAQSGRPGPVVIDVP
KDMTAPSEKFAYEYPKEVFIRSYQPSLKGHSGQIKKAVETLLAAKRPIIYSGGGVVLGNAYKELTALAHRLNLPVTNTLM
GLGAFPGSDEQFLGMLGMHGTYEANMTMHHSDVILAVGARFDDRVTNNVKKFCPNATIIHIDIDPTSISKTIFAHIPIVG
DVKSVLTDMIDIIGEDKELDQPALLDWWSQINEWRKRHGLRYDTSTDNGIKPQAVVQALDKVTNSNAIITSDVGQHQMFA
ALYYTYNEPRQWLNSGGLGTMGVGLPYAMAAKLANPERDVVCITGEGSIQMNIQELSTCLQYDLPVKILNLNNAQLGMVK
QWQDMLYEGRHAHSYMNSLPDFVKLAESYGHVGIKITDPATMEAQLAEAMAIKDRLVFIDVYVDRNEHVYPMQIAGQTMR
DMWLTKGERT

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI21361361, Length=512, Percent_Identity=27.1484375, Blast_Score=168, Evalue=1e-41,
Organism=Homo sapiens, GI93004078, Length=571, Percent_Identity=24.5183887915937, Blast_Score=167, Evalue=3e-41,
Organism=Escherichia coli, GI87081685, Length=575, Percent_Identity=61.9130434782609, Blast_Score=774, Evalue=0.0,
Organism=Escherichia coli, GI1790104, Length=562, Percent_Identity=42.3487544483986, Blast_Score=445, Evalue=1e-126,
Organism=Escherichia coli, GI1786717, Length=548, Percent_Identity=33.5766423357664, Blast_Score=323, Evalue=2e-89,
Organism=Escherichia coli, GI1787096, Length=557, Percent_Identity=28.7253141831239, Blast_Score=194, Evalue=9e-51,
Organism=Escherichia coli, GI1788716, Length=574, Percent_Identity=24.7386759581882, Blast_Score=152, Evalue=4e-38,
Organism=Caenorhabditis elegans, GI17531299, Length=589, Percent_Identity=24.6179966044143, Blast_Score=163, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI17531301, Length=589, Percent_Identity=24.6179966044143, Blast_Score=163, Evalue=3e-40,
Organism=Caenorhabditis elegans, GI17542570, Length=571, Percent_Identity=25.7443082311734, Blast_Score=149, Evalue=5e-36,
Organism=Saccharomyces cerevisiae, GI6323755, Length=584, Percent_Identity=41.7808219178082, Blast_Score=454, Evalue=1e-128,
Organism=Saccharomyces cerevisiae, GI6320816, Length=488, Percent_Identity=25.4098360655738, Blast_Score=123, Evalue=6e-29,
Organism=Saccharomyces cerevisiae, GI6321524, Length=481, Percent_Identity=22.037422037422, Blast_Score=78, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6323073, Length=478, Percent_Identity=22.1757322175732, Blast_Score=73, Evalue=1e-13,
Organism=Drosophila melanogaster, GI19922626, Length=588, Percent_Identity=25.1700680272109, Blast_Score=179, Evalue=4e-45,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 62688; Mature: 62688

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSGAEMLVQALTDEGVKYIFGYPGGAVLHIYDALFQQDNVEHILVRHEQAAGHMADAYS
CCCHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHCCCCCCEEEEEEHHHCCHHHHHHH
RVTGNTGVVLATSGPGATNTVTAIATAFMDSVPMVIIAGQVPSSLIGEDAFQETDMIGVS
HHCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCEEEEECCCCHHHHCCHHHHHCCCCCCC
RPIVKHSFQVRHASEIPMIVKKAFYIAQSGRPGPVVIDVPKDMTAPSEKFAYEYPKEVFI
CHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCHHHHHHCCHHHHH
RSYQPSLKGHSGQIKKAVETLLAAKRPIIYSGGGVVLGNAYKELTALAHRLNLPVTNTLM
CCCCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCEEECCHHHHHHHHHHHCCCCHHHHHH
GLGAFPGSDEQFLGMLGMHGTYEANMTMHHSDVILAVGARFDDRVTNNVKKFCPNATIIH
HCCCCCCCCHHHHHHHCCCCEEECCEEEECCCEEEEECCCCCHHHHHHHHHHCCCCEEEE
IDIDPTSISKTIFAHIPIVGDVKSVLTDMIDIIGEDKELDQPALLDWWSQINEWRKRHGL
EECCCCHHHHHHHEECCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCC
RYDTSTDNGIKPQAVVQALDKVTNSNAIITSDVGQHQMFAALYYTYNEPRQWLNSGGLGT
EECCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCHHEEEEEEEECCCHHHHHHCCCCCE
MGVGLPYAMAAKLANPERDVVCITGEGSIQMNIQELSTCLQYDLPVKILNLNNAQLGMVK
ECCCCHHHHHHHHCCCCCCEEEEECCCCEEEEHHHHHHHHHCCCCEEEEECCCCCHHHHH
QWQDMLYEGRHAHSYMNSLPDFVKLAESYGHVGIKITDPATMEAQLAEAMAIKDRLVFID
HHHHHHHCCHHHHHHHHHCHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHCCEEEEEE
VYVDRNEHVYPMQIAGQTMRDMWLTKGERT
EEECCCCCEEEHHHCCHHHHHHHHCCCCCC
>Mature Secondary Structure
MLSGAEMLVQALTDEGVKYIFGYPGGAVLHIYDALFQQDNVEHILVRHEQAAGHMADAYS
CCCHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHCCCCCCEEEEEEHHHCCHHHHHHH
RVTGNTGVVLATSGPGATNTVTAIATAFMDSVPMVIIAGQVPSSLIGEDAFQETDMIGVS
HHCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCEEEEECCCCHHHHCCHHHHHCCCCCCC
RPIVKHSFQVRHASEIPMIVKKAFYIAQSGRPGPVVIDVPKDMTAPSEKFAYEYPKEVFI
CHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCHHHHHHCCHHHHH
RSYQPSLKGHSGQIKKAVETLLAAKRPIIYSGGGVVLGNAYKELTALAHRLNLPVTNTLM
CCCCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCEEECCHHHHHHHHHHHCCCCHHHHHH
GLGAFPGSDEQFLGMLGMHGTYEANMTMHHSDVILAVGARFDDRVTNNVKKFCPNATIIH
HCCCCCCCCHHHHHHHCCCCEEECCEEEECCCEEEEECCCCCHHHHHHHHHHCCCCEEEE
IDIDPTSISKTIFAHIPIVGDVKSVLTDMIDIIGEDKELDQPALLDWWSQINEWRKRHGL
EECCCCHHHHHHHEECCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCC
RYDTSTDNGIKPQAVVQALDKVTNSNAIITSDVGQHQMFAALYYTYNEPRQWLNSGGLGT
EECCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCHHEEEEEEEECCCHHHHHHCCCCCE
MGVGLPYAMAAKLANPERDVVCITGEGSIQMNIQELSTCLQYDLPVKILNLNNAQLGMVK
ECCCCHHHHHHHHCCCCCCEEEEECCCCEEEEHHHHHHHHHCCCCEEEEECCCCCHHHHH
QWQDMLYEGRHAHSYMNSLPDFVKLAESYGHVGIKITDPATMEAQLAEAMAIKDRLVFID
HHHHHHHCCHHHHHHHHHCHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHCCEEEEEE
VYVDRNEHVYPMQIAGQTMRDMWLTKGERT
EEECCCCCEEEHHHCCHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6308579; 1630901; 9278503; 3891724; 9298646 [H]