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The map label for this gene is rng [H]

Identifier: 93005312

GI number: 93005312

Start: 590747

End: 592333

Strand: Direct

Name: rng [H]

Synonym: Pcryo_0482

Alternate gene names: 93005312

Gene position: 590747-592333 (Clockwise)

Preceding gene: 93005311

Following gene: 93005313

Centisome position: 19.31

GC content: 45.24

Gene sequence:

>1587_bases
ATGTCCGAAGAGCTGCTGATTAATATTAGTCCCATGGAGTCCCGTGTTGCCGTACTAGATAACGGTATCTTGGGCGAGAT
TTATATTGAGCGTCATCATAAACTGGGTTTGGTTGGTAACATTTACTTGGGTACAGTGGTACGCGTTCTGCCGGGTATGC
AGGCAGCCTTTGTCGATATTGGTCAGTCACGTACTGCATTCTTACACGTTAATGATATGCAGCGTGAACCTCGTCCAGCT
GCCAAAAGCAGTAAAGTCGAAAAGACTGATGATGAGCGCACTATTATAGAGGTAACCGCTGATGATTTAGCCATTACGCC
GCCTGTAATAAGTACGCAGAACACAGAAGTTGTACCAGTTTCGAAAACTCTCATCCAGCATCGTCTGCATGAAAGCCAAC
GTATCTTAGTTCAAGTCACCAAAGATCAGCTGGGCAGTAAAGGCGCGCGCTTAACCACCAATATATCGCTACCATCTCGC
TATCTGGTCTACTTGCCATCGAGTGAGCATATCGGTATCTCACAACGTATTGATGGCGAAGAAGAGCGTACACGCTTAAA
GACTGAGCTTACTAGCCTGATGCAAACCGTCAATCTCAAGGGCGGTCTTATTGCACGTACGGCGGCAGAGCGTGTCCCCG
TTGATAAACTTGAAGAAGACATCTACTATCTCTTGCAGCTATGGCGAACGATATGTGCCCGTCGCCAAGAAATGAAGCCT
AATCAAAGCTCAGAGCTTATTTATCAAGAATTATCGCTACCACTGCGCTCTATCCGTGATTTGGTACACGCTGGTACCGA
AAAAGTCGTGATTGATAATGCACAGATATATGAGCAGGTTAGATACTTTGCCAAAGAATTTGTGCCTTTTGTCTATGATC
GCATAGTACATTATACCGCTGAGCAGTCGCTGTTTGATGTGCACCGCGTCGAAGATGATTTACGTGATGCGCTCAAACGT
CGCGTTGATTTAAAGTCGGGCGGCTATCTGATTATTGACCAAACCGAAGCGATGACCACCATTGATGTCAATACTGGCTC
ATTCGTTGGTGGGCGTTCATTAGAAGATACCGTCTACAAAACAAATCTAGAGGCAACACACGCGATAGCCCGTCAACTGC
GCTTACGTAATCTAGGCGGTATTATTATCCTAGACTTCATCGATATGCTTGAGCAGCAGCACAAGGATGATATCTTAGAG
AGCTTGCAGTCACAGCTAGTACAAGACTATGCCAAAACCAAAATCACTCAAGTCAGTGAGCTTGGTTTGGTTGAGATGAC
CCGTAAGCGTACTCGTGAATCGCTTGGGCAGCAACTATGTGAGCCTTGCTCAACCTGTCAAGGGCGCGGCTTTGTCAAAA
CCGCTGAGACGGTCTGTTTTGAAATATTTCGTGAAATCATGCGCTGTGCTCGTACCTATAATTCTCCTAAGAAGTTTACG
GTGGTTGCCCATGCCGCAGTCATTGATTTATTACTCACCTCAGAGTCGGATACGGTAGCAGATTTAGAGTATTTGCTTGG
TAGAGTGATTACCTTTGATGTTGAGAATTTATATACCCAAGAACAATACGATATAGTATTAGATTGA

Upstream 100 bases:

>100_bases
ACGCTTGTGTCGTGGGCAAAAGCAAGTGGCATTTGTTACACTGTCAGGCTACCAAACTATGAATGCTTCACCAGAAAGCT
AAGTGATAAGAGAAAATACT

Downstream 100 bases:

>100_bases
ACATTAAGGCGGGATCTGAATAGTAAGTATTACCGTCAAATGTTGAAAACTGAGGGCATTTAATGGGACGGTTTAGCTTT
GATGATTAGTAAGCAATTGG

Product: ribonuclease

Products: NA

Alternate protein names: RNase G; Cytoplasmic axial filament protein [H]

Number of amino acids: Translated: 528; Mature: 527

Protein sequence:

>528_residues
MSEELLINISPMESRVAVLDNGILGEIYIERHHKLGLVGNIYLGTVVRVLPGMQAAFVDIGQSRTAFLHVNDMQREPRPA
AKSSKVEKTDDERTIIEVTADDLAITPPVISTQNTEVVPVSKTLIQHRLHESQRILVQVTKDQLGSKGARLTTNISLPSR
YLVYLPSSEHIGISQRIDGEEERTRLKTELTSLMQTVNLKGGLIARTAAERVPVDKLEEDIYYLLQLWRTICARRQEMKP
NQSSELIYQELSLPLRSIRDLVHAGTEKVVIDNAQIYEQVRYFAKEFVPFVYDRIVHYTAEQSLFDVHRVEDDLRDALKR
RVDLKSGGYLIIDQTEAMTTIDVNTGSFVGGRSLEDTVYKTNLEATHAIARQLRLRNLGGIIILDFIDMLEQQHKDDILE
SLQSQLVQDYAKTKITQVSELGLVEMTRKRTRESLGQQLCEPCSTCQGRGFVKTAETVCFEIFREIMRCARTYNSPKKFT
VVAHAAVIDLLLTSESDTVADLEYLLGRVITFDVENLYTQEQYDIVLD

Sequences:

>Translated_528_residues
MSEELLINISPMESRVAVLDNGILGEIYIERHHKLGLVGNIYLGTVVRVLPGMQAAFVDIGQSRTAFLHVNDMQREPRPA
AKSSKVEKTDDERTIIEVTADDLAITPPVISTQNTEVVPVSKTLIQHRLHESQRILVQVTKDQLGSKGARLTTNISLPSR
YLVYLPSSEHIGISQRIDGEEERTRLKTELTSLMQTVNLKGGLIARTAAERVPVDKLEEDIYYLLQLWRTICARRQEMKP
NQSSELIYQELSLPLRSIRDLVHAGTEKVVIDNAQIYEQVRYFAKEFVPFVYDRIVHYTAEQSLFDVHRVEDDLRDALKR
RVDLKSGGYLIIDQTEAMTTIDVNTGSFVGGRSLEDTVYKTNLEATHAIARQLRLRNLGGIIILDFIDMLEQQHKDDILE
SLQSQLVQDYAKTKITQVSELGLVEMTRKRTRESLGQQLCEPCSTCQGRGFVKTAETVCFEIFREIMRCARTYNSPKKFT
VVAHAAVIDLLLTSESDTVADLEYLLGRVITFDVENLYTQEQYDIVLD
>Mature_527_residues
SEELLINISPMESRVAVLDNGILGEIYIERHHKLGLVGNIYLGTVVRVLPGMQAAFVDIGQSRTAFLHVNDMQREPRPAA
KSSKVEKTDDERTIIEVTADDLAITPPVISTQNTEVVPVSKTLIQHRLHESQRILVQVTKDQLGSKGARLTTNISLPSRY
LVYLPSSEHIGISQRIDGEEERTRLKTELTSLMQTVNLKGGLIARTAAERVPVDKLEEDIYYLLQLWRTICARRQEMKPN
QSSELIYQELSLPLRSIRDLVHAGTEKVVIDNAQIYEQVRYFAKEFVPFVYDRIVHYTAEQSLFDVHRVEDDLRDALKRR
VDLKSGGYLIIDQTEAMTTIDVNTGSFVGGRSLEDTVYKTNLEATHAIARQLRLRNLGGIIILDFIDMLEQQHKDDILES
LQSQLVQDYAKTKITQVSELGLVEMTRKRTRESLGQQLCEPCSTCQGRGFVKTAETVCFEIFREIMRCARTYNSPKKFTV
VAHAAVIDLLLTSESDTVADLEYLLGRVITFDVENLYTQEQYDIVLD

Specific function: Involved in the processing of the 5'-end of 16S rRNA. Could be involved in chromosome segregation and cell division. It may be one of the components of the cytoplasmic axial filaments bundles or merely regulate the formation of this structure [H]

COG id: COG1530

COG function: function code J; Ribonucleases G and E

Gene ontology:

Cell location: Cytoplasm, cytoskeleton [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI87082235, Length=527, Percent_Identity=47.2485768500949, Blast_Score=450, Evalue=1e-128,
Organism=Escherichia coli, GI1787325, Length=502, Percent_Identity=34.2629482071713, Blast_Score=255, Evalue=4e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR019307
- InterPro:   IPR022967
- InterPro:   IPR004659 [H]

Pfam domain/function: PF10150 RNase_E_G; PF00575 S1 [H]

EC number: 3.1.4.- [C]

Molecular weight: Translated: 59958; Mature: 59827

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEELLINISPMESRVAVLDNGILGEIYIERHHKLGLVGNIYLGTVVRVLPGMQAAFVDI
CCCCEEEEECCCHHHHHHHCCCCEEEEEEECCCCCCEEEHHHHHHHHHHHCCCHHHHHCC
GQSRTAFLHVNDMQREPRPAAKSSKVEKTDDERTIIEVTADDLAITPPVISTQNTEVVPV
CCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCEEEEEECCCEEECCCEECCCCCEEEEH
SKTLIQHRLHESQRILVQVTKDQLGSKGARLTTNISLPSRYLVYLPSSEHIGISQRIDGE
HHHHHHHHHCCCCEEEEEEEHHHCCCCCCEEEEEECCCCEEEEEECCCCCCCCHHCCCCH
EERTRLKTELTSLMQTVNLKGGLIARTAAERVPVDKLEEDIYYLLQLWRTICARRQEMKP
HHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
NQSSELIYQELSLPLRSIRDLVHAGTEKVVIDNAQIYEQVRYFAKEFVPFVYDRIVHYTA
CCHHHHHHHHHHCHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHH
EQSLFDVHRVEDDLRDALKRRVDLKSGGYLIIDQTEAMTTIDVNTGSFVGGRSLEDTVYK
HHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCEEEEECCCCCCCCCCCCHHHHHH
TNLEATHAIARQLRLRNLGGIIILDFIDMLEQQHKDDILESLQSQLVQDYAKTKITQVSE
CCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGLVEMTRKRTRESLGQQLCEPCSTCQGRGFVKTAETVCFEIFREIMRCARTYNSPKKFT
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
VVAHAAVIDLLLTSESDTVADLEYLLGRVITFDVENLYTQEQYDIVLD
EEHHHHHHHHHHCCCCCHHHHHHHHHHHHHEEEHHHHHCCCCCCEECC
>Mature Secondary Structure 
SEELLINISPMESRVAVLDNGILGEIYIERHHKLGLVGNIYLGTVVRVLPGMQAAFVDI
CCCEEEEECCCHHHHHHHCCCCEEEEEEECCCCCCEEEHHHHHHHHHHHCCCHHHHHCC
GQSRTAFLHVNDMQREPRPAAKSSKVEKTDDERTIIEVTADDLAITPPVISTQNTEVVPV
CCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCEEEEEECCCEEECCCEECCCCCEEEEH
SKTLIQHRLHESQRILVQVTKDQLGSKGARLTTNISLPSRYLVYLPSSEHIGISQRIDGE
HHHHHHHHHCCCCEEEEEEEHHHCCCCCCEEEEEECCCCEEEEEECCCCCCCCHHCCCCH
EERTRLKTELTSLMQTVNLKGGLIARTAAERVPVDKLEEDIYYLLQLWRTICARRQEMKP
HHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
NQSSELIYQELSLPLRSIRDLVHAGTEKVVIDNAQIYEQVRYFAKEFVPFVYDRIVHYTA
CCHHHHHHHHHHCHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHH
EQSLFDVHRVEDDLRDALKRRVDLKSGGYLIIDQTEAMTTIDVNTGSFVGGRSLEDTVYK
HHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCEEEEECCCCCCCCCCCCHHHHHH
TNLEATHAIARQLRLRNLGGIIILDFIDMLEQQHKDDILESLQSQLVQDYAKTKITQVSE
CCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGLVEMTRKRTRESLGQQLCEPCSTCQGRGFVKTAETVCFEIFREIMRCARTYNSPKKFT
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
VVAHAAVIDLLLTSESDTVADLEYLLGRVITFDVENLYTQEQYDIVLD
EEHHHHHHHHHHCCCCCHHHHHHHHHHHHHEEEHHHHHCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]