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The map label for this gene is queF [H]

Identifier: 93005292

GI number: 93005292

Start: 565936

End: 566793

Strand: Direct

Name: queF [H]

Synonym: Pcryo_0462

Alternate gene names: 93005292

Gene position: 565936-566793 (Clockwise)

Preceding gene: 93005291

Following gene: 93005294

Centisome position: 18.5

GC content: 41.14

Gene sequence:

>858_bases
ATGAGTATCCACGGTATCTTGGGTGAACAAACCACTGATTATCCAACTGAGTATAGTCCAGAGACGTTATATCCGATTGC
TCGTAGTATGGGGCGAGAGGTCATTGGTTGGCAGGATGATAAGCTAGAGGTAGGTTTCGATTGGTGGCAGGCATTTGAGC
TGTCATGGTTAAATGAGCAAGGTATATCGCAGGTTGCGATAGCACGCTTTGGCATTCCGGCAAGCTCGCCATTTATTGTT
GAATCAAAGTCATTGAAGCTTTATCTAAATAGTATCAACTTTACTGAATTTGGTAGTTTGGAAACAGTACAAACGCTGAT
AGCGAAAGATTTGTCGAAATGCGTACAAGCTGAAGTTAATGTTGAATTGTTTGACTTAGAGGATGAGCATTCAGGGCTAT
TGATCGCTCAGCCTGATGGCATTTGTATTGATGATGCACTAGCGGACAGTACTGAGAAAGTAGCATTAACGCTGCATCCT
GATGCGTCATTATTAGAACGTAATACAAGTGATGCTAAAATATCAGAGGGTAAAACTTTTAGCTTTTATTCTAATCTATT
ACGTAGTAATTGTCCGGTGACCAATCAGCCAGATTGGGGGACATTGGCAGTTTCGATAACCAGTAATAAGCCAGTTAATA
ACGCAAATATGCTGCGTTATATATTAAGTTTCCGTCAGCATAATGGCTTTCATGAGCAATGTGTCGAGCAGATATTTGCT
GATTTGAGTCAATACTATGAGCCAAGTGAGCTCATGGTTCGTGCTTGGTATACACGTCGAGGTGGTATTGATATCAATCC
TTGTCGTGTCAGCGATATTGCATTATTGCCTGTGCCTAGTCGTTTGATTCGTCAATAA

Upstream 100 bases:

>100_bases
ATATAAATCTTTCATCCCGTTCGAGCACTTGAACTGGTTAATAATCTTACTAAAACTTTAAATGTTATTTAATTTCAATC
ATTACTATTAGGAAATACCA

Downstream 100 bases:

>100_bases
TAAAAGCATTATGAATGGTTTTTAAATAAACCTCTAAGAATAAAAAAGCGCCTATCTCATATACTGAGATAGGCGCTTTT
TATGGTACTAATTTTATCGT

Product: 7-cyano-7-deazaguanine reductase

Products: NA

Alternate protein names: 7-cyano-7-carbaguanine reductase; NADPH-dependent nitrile oxidoreductase; PreQ(0) reductase [H]

Number of amino acids: Translated: 285; Mature: 284

Protein sequence:

>285_residues
MSIHGILGEQTTDYPTEYSPETLYPIARSMGREVIGWQDDKLEVGFDWWQAFELSWLNEQGISQVAIARFGIPASSPFIV
ESKSLKLYLNSINFTEFGSLETVQTLIAKDLSKCVQAEVNVELFDLEDEHSGLLIAQPDGICIDDALADSTEKVALTLHP
DASLLERNTSDAKISEGKTFSFYSNLLRSNCPVTNQPDWGTLAVSITSNKPVNNANMLRYILSFRQHNGFHEQCVEQIFA
DLSQYYEPSELMVRAWYTRRGGIDINPCRVSDIALLPVPSRLIRQ

Sequences:

>Translated_285_residues
MSIHGILGEQTTDYPTEYSPETLYPIARSMGREVIGWQDDKLEVGFDWWQAFELSWLNEQGISQVAIARFGIPASSPFIV
ESKSLKLYLNSINFTEFGSLETVQTLIAKDLSKCVQAEVNVELFDLEDEHSGLLIAQPDGICIDDALADSTEKVALTLHP
DASLLERNTSDAKISEGKTFSFYSNLLRSNCPVTNQPDWGTLAVSITSNKPVNNANMLRYILSFRQHNGFHEQCVEQIFA
DLSQYYEPSELMVRAWYTRRGGIDINPCRVSDIALLPVPSRLIRQ
>Mature_284_residues
SIHGILGEQTTDYPTEYSPETLYPIARSMGREVIGWQDDKLEVGFDWWQAFELSWLNEQGISQVAIARFGIPASSPFIVE
SKSLKLYLNSINFTEFGSLETVQTLIAKDLSKCVQAEVNVELFDLEDEHSGLLIAQPDGICIDDALADSTEKVALTLHPD
ASLLERNTSDAKISEGKTFSFYSNLLRSNCPVTNQPDWGTLAVSITSNKPVNNANMLRYILSFRQHNGFHEQCVEQIFAD
LSQYYEPSELMVRAWYTRRGGIDINPCRVSDIALLPVPSRLIRQ

Specific function: Catalyzes the NADPH-dependent reduction of 7-cyano-7- deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) [H]

COG id: COG0780

COG function: function code R; Enzyme related to GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family. QueF type 2 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789158, Length=285, Percent_Identity=40.7017543859649, Blast_Score=207, Evalue=6e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016428
- InterPro:   IPR020602 [H]

Pfam domain/function: PF01227 GTP_cyclohydroI [H]

EC number: =1.7.1.13 [H]

Molecular weight: Translated: 32059; Mature: 31928

Theoretical pI: Translated: 4.41; Mature: 4.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIHGILGEQTTDYPTEYSPETLYPIARSMGREVIGWQDDKLEVGFDWWQAFELSWLNEQ
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCHHCCCCCCCEEECHHHHHHHHHHHCCCC
GISQVAIARFGIPASSPFIVESKSLKLYLNSINFTEFGSLETVQTLIAKDLSKCVQAEVN
CHHHHHHHHHCCCCCCCEEEECCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCC
VELFDLEDEHSGLLIAQPDGICIDDALADSTEKVALTLHPDASLLERNTSDAKISEGKTF
EEEEECCCCCCCEEEECCCCCEECHHHCCCCCEEEEEECCCHHHHHCCCCCCCCCCCCCH
SFYSNLLRSNCPVTNQPDWGTLAVSITSNKPVNNANMLRYILSFRQHNGFHEQCVEQIFA
HHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHH
DLSQYYEPSELMVRAWYTRRGGIDINPCRVSDIALLPVPSRLIRQ
HHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCHHHHCC
>Mature Secondary Structure 
SIHGILGEQTTDYPTEYSPETLYPIARSMGREVIGWQDDKLEVGFDWWQAFELSWLNEQ
CCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCHHCCCCCCCEEECHHHHHHHHHHHCCCC
GISQVAIARFGIPASSPFIVESKSLKLYLNSINFTEFGSLETVQTLIAKDLSKCVQAEVN
CHHHHHHHHHCCCCCCCEEEECCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCC
VELFDLEDEHSGLLIAQPDGICIDDALADSTEKVALTLHPDASLLERNTSDAKISEGKTF
EEEEECCCCCCCEEEECCCCCEECHHHCCCCCEEEEEECCCHHHHHCCCCCCCCCCCCCH
SFYSNLLRSNCPVTNQPDWGTLAVSITSNKPVNNANMLRYILSFRQHNGFHEQCVEQIFA
HHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHH
DLSQYYEPSELMVRAWYTRRGGIDINPCRVSDIALLPVPSRLIRQ
HHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA