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The map label for this gene is nylA [H]

Identifier: 93005261

GI number: 93005261

Start: 528053

End: 529546

Strand: Direct

Name: nylA [H]

Synonym: Pcryo_0431

Alternate gene names: 93005261

Gene position: 528053-529546 (Clockwise)

Preceding gene: 93005260

Following gene: 93005270

Centisome position: 17.26

GC content: 47.46

Gene sequence:

>1494_bases
ATGTTTAAAGAATATACCCAATACGATGCATTAGCACTCGCGGCATTGGTCAATGCTGGGGAAGTCAGTGCCAAAGAGTG
GCTGGATGCAGCAGTCTATCAAGCCAATCAGCTCAATCCCAAATTGAATGCTATCATTCATCGTTTTGACGAGCGCGCTT
ATGCTGCAGCGCAGGCGGGACTGCCAGCTGGTGTATTTAACGGCGTCCCTTATTTATTAAAAGATTTGTCATTTAGCTTC
ACTGATGAGCCGATTACGATGGGCAGTCGTAGCGTCAATATCATCTCAAAAAGCGATAGCGAAATCGTCAAGCGTATGAA
AGCTACGGGTGTGAACACTTTTGGTAAGACCAATACCCCAGAATTTGGCTTGATTATCACCACTGAGCCAAAAGCACATG
GCGTGACACATAATCCTTTTAAAAAAGGCTATAGCTCTGGTGGCTCATCGGGTGGTAGTGCGGCGGCAGTGGCAAGCGGT
ATCGTACCGATGGCAGGCGCGGGCGATGGTGGCGGCTCGATACGCTTTCCTGCTGCATGGTGTGGTACGTTTGGACTTAA
ACCCAGTCGTGGACGTAACCCAATGGGTCCCTTATTTGGTGAAGGCTGGGAAGGCGCAGTAGCCGATCATGTAATTACAC
GTAGCGTGCGTGATAGTGCAGCGATGCTTGATGCGACTAGTGGTGCTGAGATTGGGGCGCCTTATGTCATAGCGCCGCCT
AAAGGTACTTTCTTAGAAGCGGCGATGCGCGCTCCACGACAACTGACCATCGCCTTGCATCAACAGCCATTGGTCGCTAA
TACTATGGTCGATAAGGAAGTGCTTACCGTATTAGAGCAGACAGCTAAGCAGCTAGAAGCCATGGGTCACCGCGTAATAC
CGGCTGAACCTAATATTAATATTGAACAATTCTGGCATAATTTTTTAGTCGTTGTTTGCGCGCATACTGCGTTTACTATT
GATAATATTGAGCGTTGCTATGGTGCGCAGCATATACAAAATCTTGAACCGCAAACGTATAATATGGCAATGTTAGGACG
TTCATTATCGGCTGTTGATTTGGCGCACGCCAAGCATGGCTGGCATGATAGCCAATATCAAACCGGTCTTCTGCTAGAGA
CCTACGATATGATTTTGTGCCCAACCGTTCCAACACCGGCAGTAAAACATGGCGTATTACCACCAAGTCGCATGGACGAA
ATGCTGATGCGCAGCGCTGGTTTGCTAAATAAAGGCATGGATATGGGCAAATACGCTTTTAGCTCAGGCATGATTGAAAA
GTTAAGCTCGCCAGTGCTTGGGAAGATGGGTTTTACCTTATTGGGTAATATTACAGGCTTGCCAGCCATGTCGTTGCCTG
TCGGTATGAGTAAAAAAGGTCTGCCGATCGGTATGCAATTGATTGGGCGTATGAATGATGAGACGACGTTATTGAGTGTA
GCAGGCGAAATGGAGCGTGGAGGTTTATTTACCAAACTTGCTTTTGAAAAGTAG

Upstream 100 bases:

>100_bases
AAACAGTGAGGTTTTATACTAAACTGATTACTGTCTATAAATCGGATACATATTAATCTTTTAAAATTTTAATTTGATTA
TTCTCCAAGGAAGGTCGGTT

Downstream 100 bases:

>100_bases
TGTGGTATACCTGCATATATCTGACGCTTTGATTGAGAGCCTTTAATCAAAGCGATAGATATCCATGCCTAAGCTATGAT
GCGCCATACTTTGATGCACG

Product: amidase

Products: NA

Alternate protein names: Nylon oligomers-degrading enzyme EI [H]

Number of amino acids: Translated: 497; Mature: 497

Protein sequence:

>497_residues
MFKEYTQYDALALAALVNAGEVSAKEWLDAAVYQANQLNPKLNAIIHRFDERAYAAAQAGLPAGVFNGVPYLLKDLSFSF
TDEPITMGSRSVNIISKSDSEIVKRMKATGVNTFGKTNTPEFGLIITTEPKAHGVTHNPFKKGYSSGGSSGGSAAAVASG
IVPMAGAGDGGGSIRFPAAWCGTFGLKPSRGRNPMGPLFGEGWEGAVADHVITRSVRDSAAMLDATSGAEIGAPYVIAPP
KGTFLEAAMRAPRQLTIALHQQPLVANTMVDKEVLTVLEQTAKQLEAMGHRVIPAEPNINIEQFWHNFLVVVCAHTAFTI
DNIERCYGAQHIQNLEPQTYNMAMLGRSLSAVDLAHAKHGWHDSQYQTGLLLETYDMILCPTVPTPAVKHGVLPPSRMDE
MLMRSAGLLNKGMDMGKYAFSSGMIEKLSSPVLGKMGFTLLGNITGLPAMSLPVGMSKKGLPIGMQLIGRMNDETTLLSV
AGEMERGGLFTKLAFEK

Sequences:

>Translated_497_residues
MFKEYTQYDALALAALVNAGEVSAKEWLDAAVYQANQLNPKLNAIIHRFDERAYAAAQAGLPAGVFNGVPYLLKDLSFSF
TDEPITMGSRSVNIISKSDSEIVKRMKATGVNTFGKTNTPEFGLIITTEPKAHGVTHNPFKKGYSSGGSSGGSAAAVASG
IVPMAGAGDGGGSIRFPAAWCGTFGLKPSRGRNPMGPLFGEGWEGAVADHVITRSVRDSAAMLDATSGAEIGAPYVIAPP
KGTFLEAAMRAPRQLTIALHQQPLVANTMVDKEVLTVLEQTAKQLEAMGHRVIPAEPNINIEQFWHNFLVVVCAHTAFTI
DNIERCYGAQHIQNLEPQTYNMAMLGRSLSAVDLAHAKHGWHDSQYQTGLLLETYDMILCPTVPTPAVKHGVLPPSRMDE
MLMRSAGLLNKGMDMGKYAFSSGMIEKLSSPVLGKMGFTLLGNITGLPAMSLPVGMSKKGLPIGMQLIGRMNDETTLLSV
AGEMERGGLFTKLAFEK
>Mature_497_residues
MFKEYTQYDALALAALVNAGEVSAKEWLDAAVYQANQLNPKLNAIIHRFDERAYAAAQAGLPAGVFNGVPYLLKDLSFSF
TDEPITMGSRSVNIISKSDSEIVKRMKATGVNTFGKTNTPEFGLIITTEPKAHGVTHNPFKKGYSSGGSSGGSAAAVASG
IVPMAGAGDGGGSIRFPAAWCGTFGLKPSRGRNPMGPLFGEGWEGAVADHVITRSVRDSAAMLDATSGAEIGAPYVIAPP
KGTFLEAAMRAPRQLTIALHQQPLVANTMVDKEVLTVLEQTAKQLEAMGHRVIPAEPNINIEQFWHNFLVVVCAHTAFTI
DNIERCYGAQHIQNLEPQTYNMAMLGRSLSAVDLAHAKHGWHDSQYQTGLLLETYDMILCPTVPTPAVKHGVLPPSRMDE
MLMRSAGLLNKGMDMGKYAFSSGMIEKLSSPVLGKMGFTLLGNITGLPAMSLPVGMSKKGLPIGMQLIGRMNDETTLLSV
AGEMERGGLFTKLAFEK

Specific function: Specifically catalyzes the hydrolysis of 6-aminohexanoic acid cyclic dimer (1,8-diazacyclotetradecane-2,9-dione) to form the linear dimer 6-aminohexanoyl-6-aminohexanoic acid. Is inactive on 6-aminohexanoic acid oligomers (degree of polymerization 2 to 6)

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI195972892, Length=520, Percent_Identity=24.2307692307692, Blast_Score=85, Evalue=2e-16,
Organism=Homo sapiens, GI222831590, Length=503, Percent_Identity=24.6520874751491, Blast_Score=83, Evalue=7e-16,
Organism=Homo sapiens, GI166795287, Length=290, Percent_Identity=27.5862068965517, Blast_Score=66, Evalue=9e-11,
Organism=Caenorhabditis elegans, GI17537465, Length=197, Percent_Identity=28.9340101522843, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17556264, Length=239, Percent_Identity=31.7991631799163, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17556278, Length=444, Percent_Identity=24.3243243243243, Blast_Score=72, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI17556276, Length=312, Percent_Identity=27.2435897435897, Blast_Score=72, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI17538254, Length=432, Percent_Identity=25.6944444444444, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI45550774, Length=243, Percent_Identity=29.6296296296296, Blast_Score=83, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24648435, Length=241, Percent_Identity=29.045643153527, Blast_Score=83, Evalue=6e-16,
Organism=Drosophila melanogaster, GI24648437, Length=241, Percent_Identity=29.045643153527, Blast_Score=83, Evalue=6e-16,
Organism=Drosophila melanogaster, GI21356731, Length=235, Percent_Identity=31.4893617021277, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24644968, Length=242, Percent_Identity=27.2727272727273, Blast_Score=78, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24648113, Length=536, Percent_Identity=25.3731343283582, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24648441, Length=139, Percent_Identity=33.8129496402878, Blast_Score=69, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24648439, Length=139, Percent_Identity=33.8129496402878, Blast_Score=69, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: =3.5.2.12 [H]

Molecular weight: Translated: 53201; Mature: 53201

Theoretical pI: Translated: 7.42; Mature: 7.42

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKEYTQYDALALAALVNAGEVSAKEWLDAAVYQANQLNPKLNAIIHRFDERAYAAAQAG
CCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCC
LPAGVFNGVPYLLKDLSFSFTDEPITMGSRSVNIISKSDSEIVKRMKATGVNTFGKTNTP
CCHHHHCCHHHHHHHCCCCCCCCCEECCCCEEEEEECCHHHHHHHHHHCCCCCCCCCCCC
EFGLIITTEPKAHGVTHNPFKKGYSSGGSSGGSAAAVASGIVPMAGAGDGGGSIRFPAAW
CEEEEEECCCCCCCCCCCHHHHHHCCCCCCCCCHHHHHHCCCCCCCCCCCCCCEECCHHH
CGTFGLKPSRGRNPMGPLFGEGWEGAVADHVITRSVRDSAAMLDATSGAEIGAPYVIAPP
HHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECC
KGTFLEAAMRAPRQLTIALHQQPLVANTMVDKEVLTVLEQTAKQLEAMGHRVIPAEPNIN
CCHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCCCCC
IEQFWHNFLVVVCAHTAFTIDNIERCYGAQHIQNLEPQTYNMAMLGRSLSAVDLAHAKHG
HHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCCCEEHHHHCCCCHHHHHHHHHCC
WHDSQYQTGLLLETYDMILCPTVPTPAVKHGVLPPSRMDEMLMRSAGLLNKGMDMGKYAF
CCCCCCCCCEEEEECCEEEECCCCCHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHH
SSGMIEKLSSPVLGKMGFTLLGNITGLPAMSLPVGMSKKGLPIGMQLIGRMNDETTLLSV
HHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCEEEEE
AGEMERGGLFTKLAFEK
CCCHHHCCEEEEECCCC
>Mature Secondary Structure
MFKEYTQYDALALAALVNAGEVSAKEWLDAAVYQANQLNPKLNAIIHRFDERAYAAAQAG
CCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCC
LPAGVFNGVPYLLKDLSFSFTDEPITMGSRSVNIISKSDSEIVKRMKATGVNTFGKTNTP
CCHHHHCCHHHHHHHCCCCCCCCCEECCCCEEEEEECCHHHHHHHHHHCCCCCCCCCCCC
EFGLIITTEPKAHGVTHNPFKKGYSSGGSSGGSAAAVASGIVPMAGAGDGGGSIRFPAAW
CEEEEEECCCCCCCCCCCHHHHHHCCCCCCCCCHHHHHHCCCCCCCCCCCCCCEECCHHH
CGTFGLKPSRGRNPMGPLFGEGWEGAVADHVITRSVRDSAAMLDATSGAEIGAPYVIAPP
HHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECC
KGTFLEAAMRAPRQLTIALHQQPLVANTMVDKEVLTVLEQTAKQLEAMGHRVIPAEPNIN
CCHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCCCCC
IEQFWHNFLVVVCAHTAFTIDNIERCYGAQHIQNLEPQTYNMAMLGRSLSAVDLAHAKHG
HHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCCCEEHHHHCCCCHHHHHHHHHCC
WHDSQYQTGLLLETYDMILCPTVPTPAVKHGVLPPSRMDEMLMRSAGLLNKGMDMGKYAF
CCCCCCCCCEEEEECCEEEECCCCCHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHH
SSGMIEKLSSPVLGKMGFTLLGNITGLPAMSLPVGMSKKGLPIGMQLIGRMNDETTLLSV
HHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCEEEEE
AGEMERGGLFTKLAFEK
CCCHHHCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2722746 [H]