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The map label for this gene is pucI [H]

Identifier: 93005235

GI number: 93005235

Start: 491925

End: 493283

Strand: Direct

Name: pucI [H]

Synonym: Pcryo_0405

Alternate gene names: 93005235

Gene position: 491925-493283 (Clockwise)

Preceding gene: 93005234

Following gene: 93005238

Centisome position: 16.08

GC content: 41.87

Gene sequence:

>1359_bases
ATGGACAATATTCAAGGAGCCGTTGCTACGGCAGGCGTAAAAACAATAGAGCATAAAGGGGTTGGTGAAGAGAGCCTAGC
GCCGCAAACCACACGAATTATGGGCTCTTGGTCTTATTTATTTACTTGGTTAGGTGGGTGTGTCTCTATTGGTACCTTCA
CCGTTGGGTCAGGTTTGGTTGGTACCCTTAACTTATTACAAGTAGTGCTAGCAATCGCCATTGGTTGTACGGTTATTGGT
GCAGCGCTCATGATTAACGGTCGCGCAGGTCATAAATATGGCATCCCGTTTATGGTGCAAAGTCGTTCATCTTTTGGTTT
CACTGGTAGTCGCATACCAGCACTGGTGCGCTCTGTACCTGCGATTGTCTGGTTTGGCTTTCAGAGCTGGATTGGTGCTG
GTGCATTAAATTTGGTGTCTGATGCTTTGTTTGGCTACAGCAATATAGTCGTCTTCTTTGTTGGTTTTCAGTTTTTACAA
ATTGGTCTGTCCGTTTTGGGTTTTCATGGTATCAAATGGCTTGAAAACATTGGCTCAATTTTTATTATTGGTTCACTGAT
TTACATGTTTATTAGTGTGATTAATAAATATGGTGATGTGATTTCAACCAATCTTATCAATACAGAAGGGACTTGGGGAG
CGCCATTTTGGGGCGCAACCATGCTGTTTTTAGGCGTTTACAGTACGATGATGCTTAACGTCTCCGACTATTCGCGTGAA
CTAAAAGAAGATGTTGGTCTTTTTCGTCAAGTAGCCATCTATGCCAACTCTATCCTACCTGCAACATTGTTTATGGGTCT
AATTGGTGTGATGGTGACCAGTGCGACTGGTGAAGTTGATCCTATCAAGGTATTTTCTAGCGCCGTTGATAACAAACCTT
TATTAATCATTACGATGCTTTTCATTGCTTTTGCGCAGGTCACTACCAATATCTTAAACAACGTCGTACCGCCTGCTTAT
GCATTAATGGATATCTTCAAAATTAAGTATAAAACCGCTGCAGTTATTGTTGGGTTGCTTGCCTTTGGAACATTCCCTTG
GGAGCTAGTAAAAGATGAATCTGCTGCGGGATTAAACTTATTTATTCAAACTTACTCAGCATTTTTAGGTCCTATATTTG
CCATCTTAGTCGTTGACTACTATTTCTTACGTAAGCAAAAGCTGGATCTTGACAAGCTATATGATGAGAACGGTCCCTAT
AAGGGCGTCAACATGGCAGCTGTTATCGCTATGGTAGTGGGGATTGTAGTCGCTCTACTATTCTCAAGTATCTCTTGGTA
TGCAAGCTTAATTCCAGCAGGCGCTATCTATTGGATCTTGATGTTAAAGATGCCGGCTGCACAGCGTTTTATGTCATAA

Upstream 100 bases:

>100_bases
TTGGCGCCTATGCAACACCTATCCAGCATTAAATAAATAGAGATGGACGAAACAGCTATTAACCAATAAATATTCAACAT
TCTCAAGGAAGGAGAAGGTG

Downstream 100 bases:

>100_bases
TTTTTTCAAATGTTTAGACAAAAACCCTATAGCAGTGAGCTCTGCTATAGGGTTTTTGCGTGAGGTGCTAGACTAAGACT
ATTTAATTTCAGCCCTAAAA

Product: cytosine/purines, uracil, thiamine, allantoin permease

Products: NA

Alternate protein names: Allantoin transport protein [H]

Number of amino acids: Translated: 452; Mature: 452

Protein sequence:

>452_residues
MDNIQGAVATAGVKTIEHKGVGEESLAPQTTRIMGSWSYLFTWLGGCVSIGTFTVGSGLVGTLNLLQVVLAIAIGCTVIG
AALMINGRAGHKYGIPFMVQSRSSFGFTGSRIPALVRSVPAIVWFGFQSWIGAGALNLVSDALFGYSNIVVFFVGFQFLQ
IGLSVLGFHGIKWLENIGSIFIIGSLIYMFISVINKYGDVISTNLINTEGTWGAPFWGATMLFLGVYSTMMLNVSDYSRE
LKEDVGLFRQVAIYANSILPATLFMGLIGVMVTSATGEVDPIKVFSSAVDNKPLLIITMLFIAFAQVTTNILNNVVPPAY
ALMDIFKIKYKTAAVIVGLLAFGTFPWELVKDESAAGLNLFIQTYSAFLGPIFAILVVDYYFLRKQKLDLDKLYDENGPY
KGVNMAAVIAMVVGIVVALLFSSISWYASLIPAGAIYWILMLKMPAAQRFMS

Sequences:

>Translated_452_residues
MDNIQGAVATAGVKTIEHKGVGEESLAPQTTRIMGSWSYLFTWLGGCVSIGTFTVGSGLVGTLNLLQVVLAIAIGCTVIG
AALMINGRAGHKYGIPFMVQSRSSFGFTGSRIPALVRSVPAIVWFGFQSWIGAGALNLVSDALFGYSNIVVFFVGFQFLQ
IGLSVLGFHGIKWLENIGSIFIIGSLIYMFISVINKYGDVISTNLINTEGTWGAPFWGATMLFLGVYSTMMLNVSDYSRE
LKEDVGLFRQVAIYANSILPATLFMGLIGVMVTSATGEVDPIKVFSSAVDNKPLLIITMLFIAFAQVTTNILNNVVPPAY
ALMDIFKIKYKTAAVIVGLLAFGTFPWELVKDESAAGLNLFIQTYSAFLGPIFAILVVDYYFLRKQKLDLDKLYDENGPY
KGVNMAAVIAMVVGIVVALLFSSISWYASLIPAGAIYWILMLKMPAAQRFMS
>Mature_452_residues
MDNIQGAVATAGVKTIEHKGVGEESLAPQTTRIMGSWSYLFTWLGGCVSIGTFTVGSGLVGTLNLLQVVLAIAIGCTVIG
AALMINGRAGHKYGIPFMVQSRSSFGFTGSRIPALVRSVPAIVWFGFQSWIGAGALNLVSDALFGYSNIVVFFVGFQFLQ
IGLSVLGFHGIKWLENIGSIFIIGSLIYMFISVINKYGDVISTNLINTEGTWGAPFWGATMLFLGVYSTMMLNVSDYSRE
LKEDVGLFRQVAIYANSILPATLFMGLIGVMVTSATGEVDPIKVFSSAVDNKPLLIITMLFIAFAQVTTNILNNVVPPAY
ALMDIFKIKYKTAAVIVGLLAFGTFPWELVKDESAAGLNLFIQTYSAFLGPIFAILVVDYYFLRKQKLDLDKLYDENGPY
KGVNMAAVIAMVVGIVVALLFSSISWYASLIPAGAIYWILMLKMPAAQRFMS

Specific function: Transport of allantoin [H]

COG id: COG1953

COG function: function code FH; Cytosine/uracil/thiamine/allantoin permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine-cytosine permease family [H]

Homologues:

Organism=Escherichia coli, GI87081749, Length=483, Percent_Identity=24.4306418219462, Blast_Score=116, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6319495, Length=445, Percent_Identity=24.9438202247191, Blast_Score=109, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6322219, Length=445, Percent_Identity=24.7191011235955, Blast_Score=100, Evalue=5e-22,
Organism=Saccharomyces cerevisiae, GI6324766, Length=420, Percent_Identity=21.1904761904762, Blast_Score=64, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6324645, Length=433, Percent_Identity=20.554272517321, Blast_Score=63, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001248
- InterPro:   IPR012681 [H]

Pfam domain/function: PF02133 Transp_cyt_pur [H]

EC number: NA

Molecular weight: Translated: 48982; Mature: 48982

Theoretical pI: Translated: 8.53; Mature: 8.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCC
>Mature Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377; 11344136 [H]