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The map label for this gene is ydgK [H]

Identifier: 93005213

GI number: 93005213

Start: 468010

End: 469269

Strand: Direct

Name: ydgK [H]

Synonym: Pcryo_0383

Alternate gene names: 93005213

Gene position: 468010-469269 (Clockwise)

Preceding gene: 93005212

Following gene: 93005214

Centisome position: 15.3

GC content: 45.4

Gene sequence:

>1260_bases
ATGTCTGCTCCAAAATCCCCCCTTCCTAATAAACCGCTTGCTGCTGACCGCGTGCGCTCTGCCGATTTACCTGTCGCATG
GATTATGATGCTGGGTCTTATCGTTGCGGTAGGACCTTTATCTATTGATATGTATCTGCCAGCATTACCATCGATGGCGG
ATGACTTTGGTGTCTCGACTGCCTTTATGGCAAACTCCGTACCTGCTTACTTTGTCGGATTGGTATTTGGTCAATTGTTT
TATGGTCCCTTTAGTGACCGCGTTGGCCGCGTCAAGCCTTTATATATAGGCATGGTCTTATATGTGATTGCCTCTATTAT
TTGTGCAACCACCAACAATGAATACGTATTGTTCGCTGGTCGTACCTTGCAAGCGCTCGGTGCTTGCGTAGGGGCAGTGG
TTACCCGTGCTGCGATTCGAGACCGTCTAACCGCTAAGCAAACGGCAAAAGCGTTTTCTATCATGATTTTGGTAATGGGC
TTAGCGCCGATATTAGCGCCATCACTTGGCGCAGTGTTTTTGCAGTATTTTAGCTGGCATTCTATCTTTTGGTTTTTGGC
GGCTTTTGGCACGTTAAATTTATTACTGACCAAATTTTTCTTTTTTGAGACCCTTAGCGAAGAAAACCGCAATGTGCGTC
CTGCCAAAGAGATACTCAGTCAATATTGGGATTTGCTGAGAGATCCCACCTTTAACTATCCGGCGATTGGCGGCGGGTTA
TTGATGGGCGCAATGTTCGTTTATATCAGCTCAGCCTCTGAACTTATCATGGATACCTATGGTATGTCAGCCACACATTT
TGGCTGGCTCTTTGGTATGAATGCCGCAGGCTTTGTGGCATTGACGCAGCTTAACCAATGGCTGACCAATCGCTTCCGTA
TTTTGAGTATTTTACGTTTTGGTGCGATGATGCAGGTCATCTCAGCAGGGGCTCTATTTATCCTCGGTATTGTATTTGGC
ACTGATGCTTGGTTGCCATTGGTCTTAGTCTGTATTTTCTTTTGTATCTCAGGTCTAGGTTTGACACAGCCCAACGCTTC
AGCGATTGCGCTTGCTTTTCAAAAGCGCCGTGCTGGTATGGCCAGTGCGCTACAAGGCTCGCTTATGTTCTCAGTAGGTA
TCTTTGGTGGATTGTTATTAAACTTATTCCCCGTCAATCCAGTACTGAAAATCGGTATCGCAATGTTTGCACTGATGAGT
CTTGGTTGCTATTTAATTTGGCAGATAGACCGTAATTTGGATTTAGATAATGCAGATTAA

Upstream 100 bases:

>100_bases
TATTGAAATATAGGCAGACAGTGCGATATAAGACTGTCTGCTTGCGTAGCGGTTCGTCGATAAAAATAAAATAACAATCA
CTAGATCAAATGGTTTAACT

Downstream 100 bases:

>100_bases
TTAACTGACAAATTCTATTTATTAATATTCATCAGTAAAAAAGCATTCAGTACGTATTCATCGTCTGAATGCTTTTTTTT
ATGCTTTTTTTATTCATTGT

Product: Bcr/CflA subfamily drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 419; Mature: 418

Protein sequence:

>419_residues
MSAPKSPLPNKPLAADRVRSADLPVAWIMMLGLIVAVGPLSIDMYLPALPSMADDFGVSTAFMANSVPAYFVGLVFGQLF
YGPFSDRVGRVKPLYIGMVLYVIASIICATTNNEYVLFAGRTLQALGACVGAVVTRAAIRDRLTAKQTAKAFSIMILVMG
LAPILAPSLGAVFLQYFSWHSIFWFLAAFGTLNLLLTKFFFFETLSEENRNVRPAKEILSQYWDLLRDPTFNYPAIGGGL
LMGAMFVYISSASELIMDTYGMSATHFGWLFGMNAAGFVALTQLNQWLTNRFRILSILRFGAMMQVISAGALFILGIVFG
TDAWLPLVLVCIFFCISGLGLTQPNASAIALAFQKRRAGMASALQGSLMFSVGIFGGLLLNLFPVNPVLKIGIAMFALMS
LGCYLIWQIDRNLDLDNAD

Sequences:

>Translated_419_residues
MSAPKSPLPNKPLAADRVRSADLPVAWIMMLGLIVAVGPLSIDMYLPALPSMADDFGVSTAFMANSVPAYFVGLVFGQLF
YGPFSDRVGRVKPLYIGMVLYVIASIICATTNNEYVLFAGRTLQALGACVGAVVTRAAIRDRLTAKQTAKAFSIMILVMG
LAPILAPSLGAVFLQYFSWHSIFWFLAAFGTLNLLLTKFFFFETLSEENRNVRPAKEILSQYWDLLRDPTFNYPAIGGGL
LMGAMFVYISSASELIMDTYGMSATHFGWLFGMNAAGFVALTQLNQWLTNRFRILSILRFGAMMQVISAGALFILGIVFG
TDAWLPLVLVCIFFCISGLGLTQPNASAIALAFQKRRAGMASALQGSLMFSVGIFGGLLLNLFPVNPVLKIGIAMFALMS
LGCYLIWQIDRNLDLDNAD
>Mature_418_residues
SAPKSPLPNKPLAADRVRSADLPVAWIMMLGLIVAVGPLSIDMYLPALPSMADDFGVSTAFMANSVPAYFVGLVFGQLFY
GPFSDRVGRVKPLYIGMVLYVIASIICATTNNEYVLFAGRTLQALGACVGAVVTRAAIRDRLTAKQTAKAFSIMILVMGL
APILAPSLGAVFLQYFSWHSIFWFLAAFGTLNLLLTKFFFFETLSEENRNVRPAKEILSQYWDLLRDPTFNYPAIGGGLL
MGAMFVYISSASELIMDTYGMSATHFGWLFGMNAAGFVALTQLNQWLTNRFRILSILRFGAMMQVISAGALFILGIVFGT
DAWLPLVLVCIFFCISGLGLTQPNASAIALAFQKRRAGMASALQGSLMFSVGIFGGLLLNLFPVNPVLKIGIAMFALMSL
GCYLIWQIDRNLDLDNAD

Specific function: Involved In Sulfonamide (Sulfathiazole) And Bicyclomycin Resistance. Probable Membrane Translocase. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Bcr/CmlA family [H]

Homologues:

Organism=Escherichia coli, GI1788509, Length=361, Percent_Identity=31.3019390581717, Blast_Score=146, Evalue=3e-36,
Organism=Escherichia coli, GI1790146, Length=239, Percent_Identity=30.9623430962343, Blast_Score=111, Evalue=9e-26,
Organism=Escherichia coli, GI48994889, Length=352, Percent_Identity=26.7045454545455, Blast_Score=102, Evalue=7e-23,
Organism=Escherichia coli, GI87082312, Length=348, Percent_Identity=29.5977011494253, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI1787065, Length=344, Percent_Identity=24.7093023255814, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1790794, Length=232, Percent_Identity=22.4137931034483, Blast_Score=72, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004812
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 45636; Mature: 45505

Theoretical pI: Translated: 8.89; Mature: 8.89

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAPKSPLPNKPLAADRVRSADLPVAWIMMLGLIVAVGPLSIDMYLPALPSMADDFGVST
CCCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHCCHHHHHHHCCCCCHHHHHCCHH
AFMANSVPAYFVGLVFGQLFYGPFSDRVGRVKPLYIGMVLYVIASIICATTNNEYVLFAG
HHHHCCCHHHHHHHHHHHHHCCCHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEC
RTLQALGACVGAVVTRAAIRDRLTAKQTAKAFSIMILVMGLAPILAPSLGAVFLQYFSWH
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SIFWFLAAFGTLNLLLTKFFFFETLSEENRNVRPAKEILSQYWDLLRDPTFNYPAIGGGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHH
LMGAMFVYISSASELIMDTYGMSATHFGWLFGMNAAGFVALTQLNQWLTNRFRILSILRF
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
GAMMQVISAGALFILGIVFGTDAWLPLVLVCIFFCISGLGLTQPNASAIALAFQKRRAGM
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
ASALQGSLMFSVGIFGGLLLNLFPVNPVLKIGIAMFALMSLGCYLIWQIDRNLDLDNAD
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCC
>Mature Secondary Structure 
SAPKSPLPNKPLAADRVRSADLPVAWIMMLGLIVAVGPLSIDMYLPALPSMADDFGVST
CCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHCCHHHHHHHCCCCCHHHHHCCHH
AFMANSVPAYFVGLVFGQLFYGPFSDRVGRVKPLYIGMVLYVIASIICATTNNEYVLFAG
HHHHCCCHHHHHHHHHHHHHCCCHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEC
RTLQALGACVGAVVTRAAIRDRLTAKQTAKAFSIMILVMGLAPILAPSLGAVFLQYFSWH
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SIFWFLAAFGTLNLLLTKFFFFETLSEENRNVRPAKEILSQYWDLLRDPTFNYPAIGGGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHH
LMGAMFVYISSASELIMDTYGMSATHFGWLFGMNAAGFVALTQLNQWLTNRFRILSILRF
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
GAMMQVISAGALFILGIVFGTDAWLPLVLVCIFFCISGLGLTQPNASAIALAFQKRRAGM
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
ASALQGSLMFSVGIFGGLLLNLFPVNPVLKIGIAMFALMSLGCYLIWQIDRNLDLDNAD
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377; 9202461 [H]