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The map label for this gene is yngF [H]

Identifier: 93005212

GI number: 93005212

Start: 467000

End: 467812

Strand: Direct

Name: yngF [H]

Synonym: Pcryo_0382

Alternate gene names: 93005212

Gene position: 467000-467812 (Clockwise)

Preceding gene: 93005211

Following gene: 93005213

Centisome position: 15.26

GC content: 46.49

Gene sequence:

>813_bases
ATGCACACTATCGCCACCTACCAATATGAAACACTGCAAGTTAGCGCGACCGACGATATTTTAACCGTGACAATCAATCG
ACCTGAGAAAAAAAATGCCATGAGCTTTAAAGTGGTCGAAGAGTTGATTGCGGTAGCCGGTCGCATCAGTAAAGACAAAA
CAATACGTGCGGTGATTTTAAATGGCGCTGAAGGAACCTTCTGCGCAGGGATTGACTTGGGCGACTTAAATCACCCCAAA
AACCAAGTTTTTGCGCTATGGGAGTTGATCAAACCTTGGCAAAGCTCATTTCAGCGCGTGTGCTTAGTATGGCGTGATGT
CCCAGTACCTGTCATTGCAGTACTGGAAGGATATTGTATCGGTGCAGGCTTACAGCTAGCGCTAGCATGTGATATTCGTA
TCAGCCATCCTGATTGTAAATTGGCTATTATGGAGGCCAAATGGGGACTAGTACCAGATATGGGCTTGACGCAATCTGGT
TTTGGCGTGGTACGCGCTGATATCCTAAAAGAGCTGGCAATGACAGCCCGTATCGTTAGTGCAGAGCAGGGCAAAGAGCT
AGGATTGGTCAGTCATTGCAGTGAGACGCCACTTGAGCAAGCGCAGCAACTTGCTGCTGAATTTGCTGAACGGTCACCAG
ATGCCGTGCTGGCAAGTAAACGCGTTATCAATGCCATGTTTGAACAATCTGCAACTACTTTATATATAGAAAAAGTCTGG
CAATTAAAGATGATGTTCGGGCGCAATCGTAAACTTGCCCTTAGAAAAGCGAAGCAAGCCAGTACTGTATTTGGCAAGCG
CCAGTTCCGTTAA

Upstream 100 bases:

>100_bases
AACGATATCGTGGCAGTCAGTTATTGATAAGGTAGGAGATAGGCAAGGCTGTTAATATCACTCTATATATAAGCTATTAT
TTCAATTATTAAGGATGATT

Downstream 100 bases:

>100_bases
TACAGTTTAGATAGCTTATTGTTTTAAATGACTTCATCTTATTTCAAACGAACCATTGTTTTATTGATTAAAGTTTGTTT
TATTAACGTGATACCTCTAT

Product: enoyl-CoA hydratase

Products: trans(or 3)-enoyl-CoA; H2O [C]

Alternate protein names: NA

Number of amino acids: Translated: 270; Mature: 270

Protein sequence:

>270_residues
MHTIATYQYETLQVSATDDILTVTINRPEKKNAMSFKVVEELIAVAGRISKDKTIRAVILNGAEGTFCAGIDLGDLNHPK
NQVFALWELIKPWQSSFQRVCLVWRDVPVPVIAVLEGYCIGAGLQLALACDIRISHPDCKLAIMEAKWGLVPDMGLTQSG
FGVVRADILKELAMTARIVSAEQGKELGLVSHCSETPLEQAQQLAAEFAERSPDAVLASKRVINAMFEQSATTLYIEKVW
QLKMMFGRNRKLALRKAKQASTVFGKRQFR

Sequences:

>Translated_270_residues
MHTIATYQYETLQVSATDDILTVTINRPEKKNAMSFKVVEELIAVAGRISKDKTIRAVILNGAEGTFCAGIDLGDLNHPK
NQVFALWELIKPWQSSFQRVCLVWRDVPVPVIAVLEGYCIGAGLQLALACDIRISHPDCKLAIMEAKWGLVPDMGLTQSG
FGVVRADILKELAMTARIVSAEQGKELGLVSHCSETPLEQAQQLAAEFAERSPDAVLASKRVINAMFEQSATTLYIEKVW
QLKMMFGRNRKLALRKAKQASTVFGKRQFR
>Mature_270_residues
MHTIATYQYETLQVSATDDILTVTINRPEKKNAMSFKVVEELIAVAGRISKDKTIRAVILNGAEGTFCAGIDLGDLNHPK
NQVFALWELIKPWQSSFQRVCLVWRDVPVPVIAVLEGYCIGAGLQLALACDIRISHPDCKLAIMEAKWGLVPDMGLTQSG
FGVVRADILKELAMTARIVSAEQGKELGLVSHCSETPLEQAQQLAAEFAERSPDAVLASKRVINAMFEQSATTLYIEKVW
QLKMMFGRNRKLALRKAKQASTVFGKRQFR

Specific function: Could Possibly Oxidizes Fatty Acids Using Specific Components (By Similarity). [C]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI70995211, Length=273, Percent_Identity=35.1648351648352, Blast_Score=120, Evalue=1e-27,
Organism=Homo sapiens, GI194097323, Length=212, Percent_Identity=31.1320754716981, Blast_Score=95, Evalue=7e-20,
Organism=Homo sapiens, GI4502327, Length=239, Percent_Identity=31.3807531380753, Blast_Score=89, Evalue=4e-18,
Organism=Homo sapiens, GI68989263, Length=194, Percent_Identity=27.8350515463918, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI20127408, Length=218, Percent_Identity=28.4403669724771, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI157694516, Length=232, Percent_Identity=22.4137931034483, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI213417737, Length=232, Percent_Identity=22.4137931034483, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1787659, Length=223, Percent_Identity=28.2511210762332, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI221142681, Length=237, Percent_Identity=28.2700421940928, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1787660, Length=229, Percent_Identity=32.3144104803493, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1788682, Length=177, Percent_Identity=29.9435028248588, Blast_Score=71, Evalue=6e-14,
Organism=Escherichia coli, GI1790281, Length=190, Percent_Identity=26.3157894736842, Blast_Score=71, Evalue=9e-14,
Organism=Escherichia coli, GI1788597, Length=227, Percent_Identity=30.8370044052863, Blast_Score=69, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17540714, Length=215, Percent_Identity=32.5581395348837, Blast_Score=118, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI17536985, Length=246, Percent_Identity=28.0487804878049, Blast_Score=106, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17534483, Length=256, Percent_Identity=26.5625, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17554946, Length=218, Percent_Identity=26.605504587156, Blast_Score=91, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI25145438, Length=228, Percent_Identity=27.1929824561404, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17560910, Length=217, Percent_Identity=27.1889400921659, Blast_Score=81, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI25141363, Length=244, Percent_Identity=25, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI25144276, Length=257, Percent_Identity=27.6264591439689, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17508951, Length=257, Percent_Identity=27.6264591439689, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17508953, Length=257, Percent_Identity=27.6264591439689, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17558304, Length=186, Percent_Identity=25.2688172043011, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24653139, Length=244, Percent_Identity=30.7377049180328, Blast_Score=112, Evalue=4e-25,
Organism=Drosophila melanogaster, GI20129971, Length=214, Percent_Identity=28.5046728971963, Blast_Score=91, Evalue=9e-19,
Organism=Drosophila melanogaster, GI24653477, Length=214, Percent_Identity=28.5046728971963, Blast_Score=91, Evalue=9e-19,
Organism=Drosophila melanogaster, GI21357171, Length=239, Percent_Identity=28.0334728033473, Blast_Score=87, Evalue=9e-18,
Organism=Drosophila melanogaster, GI19920382, Length=237, Percent_Identity=29.535864978903, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24650670, Length=223, Percent_Identity=29.1479820627803, Blast_Score=78, Evalue=7e-15,
Organism=Drosophila melanogaster, GI24583165, Length=230, Percent_Identity=24.7826086956522, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI19921018, Length=210, Percent_Identity=23.8095238095238, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: 4.2.1.17 [C]

Molecular weight: Translated: 29949; Mature: 29949

Theoretical pI: Translated: 8.81; Mature: 8.81

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHTIATYQYETLQVSATDDILTVTINRPEKKNAMSFKVVEELIAVAGRISKDKTIRAVIL
CCCEEEEEEEEEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEE
NGAEGTFCAGIDLGDLNHPKNQVFALWELIKPWQSSFQRVCLVWRDVPVPVIAVLEGYCI
ECCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCHHC
GAGLQLALACDIRISHPDCKLAIMEAKWGLVPDMGLTQSGFGVVRADILKELAMTARIVS
CCCCEEEEEEEEEECCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
AEQGKELGLVSHCSETPLEQAQQLAAEFAERSPDAVLASKRVINAMFEQSATTLYIEKVW
HHCCCCCCHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCEEEHHHHH
QLKMMFGRNRKLALRKAKQASTVFGKRQFR
HHHHHHCCCCHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MHTIATYQYETLQVSATDDILTVTINRPEKKNAMSFKVVEELIAVAGRISKDKTIRAVIL
CCCEEEEEEEEEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEE
NGAEGTFCAGIDLGDLNHPKNQVFALWELIKPWQSSFQRVCLVWRDVPVPVIAVLEGYCI
ECCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCHHC
GAGLQLALACDIRISHPDCKLAIMEAKWGLVPDMGLTQSGFGVVRADILKELAMTARIVS
CCCCEEEEEEEEEECCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
AEQGKELGLVSHCSETPLEQAQQLAAEFAERSPDAVLASKRVINAMFEQSATTLYIEKVW
HHCCCCCCHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCEEEHHHHH
QLKMMFGRNRKLALRKAKQASTVFGKRQFR
HHHHHHCCCCHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.05 {crotonyl-CoA}} 0.008 {2-decenoyl-CoA}} [C]

Substrates: (3S)-hydroxyacyl-CoA [C]

Specific reaction: (3S)-hydroxyacyl-CoA = trans(or 3)-enoyl-CoA + H2O [C]

General reaction: addition (of H2O to carbon carbon double bondelimination (of H2O C-O bond cleavagehydration [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9387222; 9384377 [H]