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The map label for this gene is pdxJ

Identifier: 93005200

GI number: 93005200

Start: 448622

End: 449416

Strand: Direct

Name: pdxJ

Synonym: Pcryo_0370

Alternate gene names: 93005200

Gene position: 448622-449416 (Clockwise)

Preceding gene: 93005199

Following gene: 93005202

Centisome position: 14.66

GC content: 44.78

Gene sequence:

>795_bases
ATGACGGATACAGCGCAGATTTTGCCGACTACATTAGAGCAAAATCCACAAAATACTTCAAAAAAACCACTCTTAGGTGT
CAACGTTGACCATGTGGCAACCTTACGTCAAGCACGCGGTGTGGGATATCCAAGCCCACTAGCTGCAGCACTTTTATGTG
AGAAAGCAGGTGCTGACGGTATCACTATTCATTTGCGTGAAGATCGCCGTCATATTCAAGATGCCGATGTGTATGAGATG
GCAGGGCAGTTGACTACGAGAATGAATCTAGAGATGGCAGCAACGACTGAGATGCTAGACATAGCCTGTCAAGTCAAACC
ATATTGGGTATGCCTAGTGCCGGAAAAACGTGCTGAACTGACTACAGAAGGGGGGCTTGATGTGGCAGGGCAGCTAGCTT
CATTGACAGATTATGTCAGTCAGTTACAAGCAGCAGGTATCAAAGTCTCTTTGTTTATTGATCCAGAAGATAAGCAGATC
GAGGCAGCAGTTAATTGTAGCGCTGACGCAATCGAATTACACACAGGTTCTTATGCTGAAGCAGGTCTGGCAGGTAAAAC
AGATAGGGTAAATGTAGAGCTTGAACGTATTAAAAATGCAGTGATTACCGCTAAACGACTTGATAGTAAGCTGTTGGTCA
ATGCTGGTCACGGTCTAACACGTGATAATGTTCATGCGATTGCCCAAATTGACGGTATATATGAGCTAAATATTGGTCAT
GCTTTGATAGCGGATGCGCTATTTGTGGGGATAGAGCAGGCGGTTATTATGATGAAAACAGCGATGCACCGATAG

Upstream 100 bases:

>100_bases
TCAACTATAAAGCGTATTTAACAATAGAATATGTTCACAACTACTTATCATCAATAAGCTATTTTTAGTAAATGATCTAA
CTATTAATAAAAGGATTTTT

Downstream 100 bases:

>100_bases
CTACGACAGTTTATTCTTAGCTTATATTGATATATCATTATGTACCACATCCGGTCGGATGGTTTTCGTTAGGTTTGTAG
GTAATACCTCCTGTCTGATT

Product: pyridoxine 5'-phosphate synthase

Products: NA

Alternate protein names: PNP synthase

Number of amino acids: Translated: 264; Mature: 263

Protein sequence:

>264_residues
MTDTAQILPTTLEQNPQNTSKKPLLGVNVDHVATLRQARGVGYPSPLAAALLCEKAGADGITIHLREDRRHIQDADVYEM
AGQLTTRMNLEMAATTEMLDIACQVKPYWVCLVPEKRAELTTEGGLDVAGQLASLTDYVSQLQAAGIKVSLFIDPEDKQI
EAAVNCSADAIELHTGSYAEAGLAGKTDRVNVELERIKNAVITAKRLDSKLLVNAGHGLTRDNVHAIAQIDGIYELNIGH
ALIADALFVGIEQAVIMMKTAMHR

Sequences:

>Translated_264_residues
MTDTAQILPTTLEQNPQNTSKKPLLGVNVDHVATLRQARGVGYPSPLAAALLCEKAGADGITIHLREDRRHIQDADVYEM
AGQLTTRMNLEMAATTEMLDIACQVKPYWVCLVPEKRAELTTEGGLDVAGQLASLTDYVSQLQAAGIKVSLFIDPEDKQI
EAAVNCSADAIELHTGSYAEAGLAGKTDRVNVELERIKNAVITAKRLDSKLLVNAGHGLTRDNVHAIAQIDGIYELNIGH
ALIADALFVGIEQAVIMMKTAMHR
>Mature_263_residues
TDTAQILPTTLEQNPQNTSKKPLLGVNVDHVATLRQARGVGYPSPLAAALLCEKAGADGITIHLREDRRHIQDADVYEMA
GQLTTRMNLEMAATTEMLDIACQVKPYWVCLVPEKRAELTTEGGLDVAGQLASLTDYVSQLQAAGIKVSLFIDPEDKQIE
AAVNCSADAIELHTGSYAEAGLAGKTDRVNVELERIKNAVITAKRLDSKLLVNAGHGLTRDNVHAIAQIDGIYELNIGHA
LIADALFVGIEQAVIMMKTAMHR

Specific function: Catalyzes the complicated ring closure reaction between the two acyclic compounds 1-deoxy-D-xylulose-5-phosphate (DXP) and 3-amino-2-oxopropyl phosphate (1-amino-acetone-3-phosphate or AAP) to form pyridoxine 5'-phosphate (PNP) and inorganic phosphate

COG id: COG0854

COG function: function code H; Pyridoxal phosphate biosynthesis protein

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNP synthase family

Homologues:

Organism=Escherichia coli, GI1788917, Length=240, Percent_Identity=57.9166666666667, Blast_Score=254, Evalue=4e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PDXJ_PSYCK (Q1QDV0)

Other databases:

- EMBL:   CP000323
- RefSeq:   YP_579637.1
- ProteinModelPortal:   Q1QDV0
- SMR:   Q1QDV0
- STRING:   Q1QDV0
- GeneID:   4034545
- GenomeReviews:   CP000323_GR
- KEGG:   pcr:Pcryo_0370
- NMPDR:   fig|335284.3.peg.1058
- eggNOG:   COG0854
- HOGENOM:   HBG299314
- OMA:   AGHGLNY
- PhylomeDB:   Q1QDV0
- ProtClustDB:   PRK05265
- BioCyc:   PCRY335284:PCRYO_0370-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00279
- InterPro:   IPR013785
- InterPro:   IPR004569
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00559

Pfam domain/function: PF03740 PdxJ; SSF63892 PyrdxlP_synth_PdxJ

EC number: =2.6.99.2

Molecular weight: Translated: 28519; Mature: 28388

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: NA

Important sites: ACT_SITE 64-64 ACT_SITE 91-91 ACT_SITE 217-217 BINDING 28-28 BINDING 39-39 BINDING 66-66 BINDING 71-71 BINDING 121-121 BINDING 218-218

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDTAQILPTTLEQNPQNTSKKPLLGVNVDHVATLRQARGVGYPSPLAAALLCEKAGADG
CCCCHHHCCHHHCCCCCCCCCCCEEECCHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCC
ITIHLREDRRHIQDADVYEMAGQLTTRMNLEMAATTEMLDIACQVKPYWVCLVPEKRAEL
EEEEEECCCCCCCCCHHHHHHHHHHHHCCEEHHHHHHHHHEEEECCCEEEEEECCCCCCC
TTEGGLDVAGQLASLTDYVSQLQAAGIKVSLFIDPEDKQIEAAVNCSADAIELHTGSYAE
CCCCCCCHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCEEEEEECCCCCEEEEECCCCCC
AGLAGKTDRVNVELERIKNAVITAKRLDSKLLVNAGHGLTRDNVHAIAQIDGIYELNIGH
CCCCCCCCEEEEEHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCEEEEEECCEEEECCCH
ALIADALFVGIEQAVIMMKTAMHR
HHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TDTAQILPTTLEQNPQNTSKKPLLGVNVDHVATLRQARGVGYPSPLAAALLCEKAGADG
CCCHHHCCHHHCCCCCCCCCCCEEECCHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCC
ITIHLREDRRHIQDADVYEMAGQLTTRMNLEMAATTEMLDIACQVKPYWVCLVPEKRAEL
EEEEEECCCCCCCCCHHHHHHHHHHHHCCEEHHHHHHHHHEEEECCCEEEEEECCCCCCC
TTEGGLDVAGQLASLTDYVSQLQAAGIKVSLFIDPEDKQIEAAVNCSADAIELHTGSYAE
CCCCCCCHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCEEEEEECCCCCEEEEECCCCCC
AGLAGKTDRVNVELERIKNAVITAKRLDSKLLVNAGHGLTRDNVHAIAQIDGIYELNIGH
CCCCCCCCEEEEEHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCEEEEEECCEEEECCCH
ALIADALFVGIEQAVIMMKTAMHR
HHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA