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The map label for this gene is gloA [H]

Identifier: 93005072

GI number: 93005072

Start: 283525

End: 284091

Strand: Direct

Name: gloA [H]

Synonym: Pcryo_0241

Alternate gene names: 93005072

Gene position: 283525-284091 (Clockwise)

Preceding gene: 93005071

Following gene: 93005076

Centisome position: 9.27

GC content: 39.68

Gene sequence:

>567_bases
ATGAGTAATGATACTTTTAACAACGACCATTCAATACAAGAGCAACCTGAAAGCAGCGTAAAGGCTAATGGCGTACAGCC
TATCTCAGCGCAGACGACAGGTTATACCTTTAACCATACGATGCTGCGTGTTAAAGATCCTGTGAAGTCTTTAGCGTTTT
ATACTGGTGTCTTAGGTATGACGTTACTGGCAGTCAAAAAATTCCCAGCCATGGGTTTTGATTTGTACTTTTTAGCCAAG
CTGACTGAGAGTGAACGTGAAAACTTGCCGTCAGGTAATGATTTAGAGATTTTTGCCTTCCGTCAGCGTGGCATTTTGGA
ATTGACTCATAACTATGGTACCGAAACAAAAGTGGACTTTAGCTATCATGATGGCAATCAAGAACCACAAGGGTTTGGTC
ATATTTGTTTTAATGTGCCTGATCTCAATGCAGCGGTAGCTTGGTTTGATGAAAACAATGTCGAATTCAAAAAACGTCCA
GATGAAGGCAGCATGAAAAATATTGTCTTTATTAAAGATGTTGATGGTTATTGGATTGAAATCGTACAAGCAGACTTGAT
GGCTTAA

Upstream 100 bases:

>100_bases
TTAATAGTGTTCTATAGAGGCTATGCTAGAAAGACTAAGTGTTATAGGTATGATCATTTAATCTATTAGTGATTAAAAAT
ATTTACATATAGGAATAATC

Downstream 100 bases:

>100_bases
TTGATTCATAAGTTAAGCGAATAAAAAAGCCACCTTATTGTTTGATAGGGTGGCTTTTTATATTGCTAAAGGATTCTTAT
TTAGTCTTTTTCGACTTGGA

Product: glyoxalase I

Products: NA

Alternate protein names: Aldoketomutase; Glyoxalase I; Glx I; Ketone-aldehyde mutase; Methylglyoxalase; S-D-lactoylglutathione methylglyoxal lyase [H]

Number of amino acids: Translated: 188; Mature: 187

Protein sequence:

>188_residues
MSNDTFNNDHSIQEQPESSVKANGVQPISAQTTGYTFNHTMLRVKDPVKSLAFYTGVLGMTLLAVKKFPAMGFDLYFLAK
LTESERENLPSGNDLEIFAFRQRGILELTHNYGTETKVDFSYHDGNQEPQGFGHICFNVPDLNAAVAWFDENNVEFKKRP
DEGSMKNIVFIKDVDGYWIEIVQADLMA

Sequences:

>Translated_188_residues
MSNDTFNNDHSIQEQPESSVKANGVQPISAQTTGYTFNHTMLRVKDPVKSLAFYTGVLGMTLLAVKKFPAMGFDLYFLAK
LTESERENLPSGNDLEIFAFRQRGILELTHNYGTETKVDFSYHDGNQEPQGFGHICFNVPDLNAAVAWFDENNVEFKKRP
DEGSMKNIVFIKDVDGYWIEIVQADLMA
>Mature_187_residues
SNDTFNNDHSIQEQPESSVKANGVQPISAQTTGYTFNHTMLRVKDPVKSLAFYTGVLGMTLLAVKKFPAMGFDLYFLAKL
TESERENLPSGNDLEIFAFRQRGILELTHNYGTETKVDFSYHDGNQEPQGFGHICFNVPDLNAAVAWFDENNVEFKKRPD
EGSMKNIVFIKDVDGYWIEIVQADLMA

Specific function: Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione [H]

COG id: COG0346

COG function: function code E; Lactoylglutathione lyase and related lyases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyoxalase I family [H]

Homologues:

Organism=Homo sapiens, GI118402586, Length=158, Percent_Identity=50.6329113924051, Blast_Score=158, Evalue=2e-39,
Organism=Escherichia coli, GI1787940, Length=147, Percent_Identity=31.9727891156463, Blast_Score=67, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6323639, Length=147, Percent_Identity=45.578231292517, Blast_Score=135, Evalue=3e-33,
Organism=Drosophila melanogaster, GI20129743, Length=160, Percent_Identity=48.75, Blast_Score=153, Evalue=6e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004360
- InterPro:   IPR004361
- InterPro:   IPR018146 [H]

Pfam domain/function: PF00903 Glyoxalase [H]

EC number: =4.4.1.5 [H]

Molecular weight: Translated: 21240; Mature: 21109

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: PS00934 GLYOXALASE_I_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNDTFNNDHSIQEQPESSVKANGVQPISAQTTGYTFNHTMLRVKDPVKSLAFYTGVLGM
CCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCEEECCEEEEECCHHHHHHHHHHHHHH
TLLAVKKFPAMGFDLYFLAKLTESERENLPSGNDLEIFAFRQRGILELTHNYGTETKVDF
HHHHHHHCCCCCCCHHHEEHHHHHHHHCCCCCCCEEEEEECCCCEEEEECCCCCCEEEEE
SYHDGNQEPQGFGHICFNVPDLNAAVAWFDENNVEFKKRPDEGSMKNIVFIKDVDGYWIE
EECCCCCCCCCCCEEEEECCCCCEEEEEEECCCCEEEECCCCCCCCEEEEEEECCCEEEE
IVQADLMA
EEEHHHCC
>Mature Secondary Structure 
SNDTFNNDHSIQEQPESSVKANGVQPISAQTTGYTFNHTMLRVKDPVKSLAFYTGVLGM
CCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCEEECCEEEEECCHHHHHHHHHHHHHH
TLLAVKKFPAMGFDLYFLAKLTESERENLPSGNDLEIFAFRQRGILELTHNYGTETKVDF
HHHHHHHCCCCCCCHHHEEHHHHHHHHCCCCCCCEEEEEECCCCEEEEECCCCCCEEEEE
SYHDGNQEPQGFGHICFNVPDLNAAVAWFDENNVEFKKRPDEGSMKNIVFIKDVDGYWIE
EECCCCCCCCCCCEEEEECCCCCEEEEEEECCCCEEEECCCCCCCCEEEEEEECCCEEEE
IVQADLMA
EEEHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]