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The map label for this gene is pnp [H]

Identifier: 93004911

GI number: 93004911

Start: 91568

End: 93670

Strand: Direct

Name: pnp [H]

Synonym: Pcryo_0080

Alternate gene names: 93004911

Gene position: 91568-93670 (Clockwise)

Preceding gene: 93004910

Following gene: 93004916

Centisome position: 2.99

GC content: 44.56

Gene sequence:

>2103_bases
ATGACAATGTTTAATACCATTAAGCGTGAATTCCAATACGGTAACCAGCAAGTTGTTATCGAAACAGGACGTATTGCGCG
TCAAGCTAACTCGATTTTAGTCCACATGGGCGGCGTTACTGTATTGGTAGCAGCGGTTGTAAAGTCTGATGCTAAAGAAG
GTCAAAACTTCTTTCCATTGACCGTTAACTATCAAGAAAAAATGTATGCTGCGGGTAAAATCCCAGGTGCTTACGGTAAA
CGTGAAGGTCGTGCGAGCGAGTCTGAAACGTTAACCTCGCGTCTAATTGACCGCCCAATCCGTCCGTTATTCCCTGAAGG
CTATGTCAACGAGATTCAAATCACGGCAACGGTTGTGTCATCTGACAAAACTCAATCAGCAGATATCGCAGCACTAATCG
GTGCTTCAGCGGCGCTAGCTATCTCTGATGCACCATTTAATGGTCCTGTTGCTGCGGCGCGTGTTGGTTTTATCAATGGC
GAATACGTACTGAACCCGACGCTTGAAGAGCTTAAAGAAAGTGATCTTGACTTGGTCGTGGCTGGTACAAAGTCTGCGGT
ATTGATGGTTGAATCTGAAGCGGCAGAGTTATCAGAAGATCAAATGCTAGGCGCAGTATTATACGGTCATCAGCAGCAGC
AAATCGTTATCGACAACATTGCTTCAATGGCAGCAGAAATCGGTACCGCTAAGCAGCAATATACCGCTCCTGTACGCAAT
CAAACGTTAGAGACTGGTATGAAAGAGCAGTTCGGTACGCAAGTATCTGACGCTTATACCATCACTGATAAGCAAGCACG
TTACAGCAAGCTTGATGAAATTAAAGACGCTGCCCTTGCAGCTCTTGCTGGTGATGCTGAATCAGAGAGCTATGCTGATA
CCGTATCTGAGTTAAAAGAAATCTATAACGATCTAAAGTATCGTACCGTTCGTGACAACATTTTGTCTGGTAAGCCACGT
ATTGATGGTCGTGATTTAGAGACTGTTCGTGCCCTTGATGTACAAGTGGGCGTATTACCATTTACTCATGGTTCAGCATT
GTTTACACGTGGTGAAACCCAAGCATTGGTAACGACTACCCTTGGTAATACTCGTGACGTCAATATGATTGACTCGCTAG
CGGGTACCATTCGTGACCATTTCATGTTGCATTATAACTTCCCACATTTCTCAGTAGGCGAAACAGGTCGTGAAGGCATT
CCTAAACGTCGTGAAATCGGTCATGGTCGTCTAGCACGCCGTGGTGTACAAGCGATGCTGCCTGATAGCGATCGCTTCCC
ATATGTTATCCGTGTGGTTTCTGAGATCACTGAATCAAATGGTTCATCGTCTATGGCCTCTGTTTGTGGTGCAAGTTTGG
CATTGATGGACGCTGGTGTGCCAATTAAAGCACCGGTTGCTGGTATTGCCATGGGTCTGGTTAAAGAAGGCGAGCGCTTT
GCTGTATTGTCTGACATCTTAGGTGATGAAGATCATCTTGGCGATATGGATTTTAAAGTTGCTGGTTCTAAAGACGGTAT
TACTGCTCTACAGATGGACATTAAAATCGAAGGTATTACCCCAGATATCATGGAGCAAGCGTTAAAGCAAGCTCATGCTG
GTCGTATCCATATCTTAGACGCGATGAACAAAGTATTGCCTGAAAGCCGTACTGAAATCAATGCTCATGCACCTAACTAT
GCCGTAATTGAGATCAATCCGGATAAAATCCGTGATGTTATCGGTAAAGGCGGCGCAACGATTCGTCAGCTAACGGAAGA
AACTGGTGCGGTTATCGACATTGATGATGCTGGTACCATCCGTATCTTTGGTGAAAACAAAGCGGCAACTAAAGCGGCAA
TTGCTAAAATTGAAGCAATCACAGCAGAAGTTGAAGTAGGTAAAACTTACGAAGGTACGGTTGCTCGTATCGTTGACTTC
GGAGCCTTTATTAACGTATTGCCAAATACTGATGGTCTCGTGCATATCTCGCAAATCGCTGATGAGCGCGTAGAAAACGT
ATCTGACTATCTAAAAGAAGGTCAAATCGTTAAAGTATTGGTCCAAGATGTTGATAATCGTGGTCGTATCAAGTTGACTA
TGAAAGGTATTGAGCAGAGCTAA

Upstream 100 bases:

>100_bases
AAAGACAGTCGATTAGAAGCTATATACAAAGAATTTTAATGACAGATATCAGTGTTTTTTGTGCTGATATTATTTCGTCA
GTCAATATTAGGAAAATTAT

Downstream 100 bases:

>100_bases
TGCTTGATATTTGATAGCGTTAATATTTAAAGAACCGCCTTGAATCACTTATTATGAGTGCTCAAGGCGGTTTTTTTATG
GACGTAAAATATAACTAAAA

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase [H]

Number of amino acids: Translated: 700; Mature: 699

Protein sequence:

>700_residues
MTMFNTIKREFQYGNQQVVIETGRIARQANSILVHMGGVTVLVAAVVKSDAKEGQNFFPLTVNYQEKMYAAGKIPGAYGK
REGRASESETLTSRLIDRPIRPLFPEGYVNEIQITATVVSSDKTQSADIAALIGASAALAISDAPFNGPVAAARVGFING
EYVLNPTLEELKESDLDLVVAGTKSAVLMVESEAAELSEDQMLGAVLYGHQQQQIVIDNIASMAAEIGTAKQQYTAPVRN
QTLETGMKEQFGTQVSDAYTITDKQARYSKLDEIKDAALAALAGDAESESYADTVSELKEIYNDLKYRTVRDNILSGKPR
IDGRDLETVRALDVQVGVLPFTHGSALFTRGETQALVTTTLGNTRDVNMIDSLAGTIRDHFMLHYNFPHFSVGETGREGI
PKRREIGHGRLARRGVQAMLPDSDRFPYVIRVVSEITESNGSSSMASVCGASLALMDAGVPIKAPVAGIAMGLVKEGERF
AVLSDILGDEDHLGDMDFKVAGSKDGITALQMDIKIEGITPDIMEQALKQAHAGRIHILDAMNKVLPESRTEINAHAPNY
AVIEINPDKIRDVIGKGGATIRQLTEETGAVIDIDDAGTIRIFGENKAATKAAIAKIEAITAEVEVGKTYEGTVARIVDF
GAFINVLPNTDGLVHISQIADERVENVSDYLKEGQIVKVLVQDVDNRGRIKLTMKGIEQS

Sequences:

>Translated_700_residues
MTMFNTIKREFQYGNQQVVIETGRIARQANSILVHMGGVTVLVAAVVKSDAKEGQNFFPLTVNYQEKMYAAGKIPGAYGK
REGRASESETLTSRLIDRPIRPLFPEGYVNEIQITATVVSSDKTQSADIAALIGASAALAISDAPFNGPVAAARVGFING
EYVLNPTLEELKESDLDLVVAGTKSAVLMVESEAAELSEDQMLGAVLYGHQQQQIVIDNIASMAAEIGTAKQQYTAPVRN
QTLETGMKEQFGTQVSDAYTITDKQARYSKLDEIKDAALAALAGDAESESYADTVSELKEIYNDLKYRTVRDNILSGKPR
IDGRDLETVRALDVQVGVLPFTHGSALFTRGETQALVTTTLGNTRDVNMIDSLAGTIRDHFMLHYNFPHFSVGETGREGI
PKRREIGHGRLARRGVQAMLPDSDRFPYVIRVVSEITESNGSSSMASVCGASLALMDAGVPIKAPVAGIAMGLVKEGERF
AVLSDILGDEDHLGDMDFKVAGSKDGITALQMDIKIEGITPDIMEQALKQAHAGRIHILDAMNKVLPESRTEINAHAPNY
AVIEINPDKIRDVIGKGGATIRQLTEETGAVIDIDDAGTIRIFGENKAATKAAIAKIEAITAEVEVGKTYEGTVARIVDF
GAFINVLPNTDGLVHISQIADERVENVSDYLKEGQIVKVLVQDVDNRGRIKLTMKGIEQS
>Mature_699_residues
TMFNTIKREFQYGNQQVVIETGRIARQANSILVHMGGVTVLVAAVVKSDAKEGQNFFPLTVNYQEKMYAAGKIPGAYGKR
EGRASESETLTSRLIDRPIRPLFPEGYVNEIQITATVVSSDKTQSADIAALIGASAALAISDAPFNGPVAAARVGFINGE
YVLNPTLEELKESDLDLVVAGTKSAVLMVESEAAELSEDQMLGAVLYGHQQQQIVIDNIASMAAEIGTAKQQYTAPVRNQ
TLETGMKEQFGTQVSDAYTITDKQARYSKLDEIKDAALAALAGDAESESYADTVSELKEIYNDLKYRTVRDNILSGKPRI
DGRDLETVRALDVQVGVLPFTHGSALFTRGETQALVTTTLGNTRDVNMIDSLAGTIRDHFMLHYNFPHFSVGETGREGIP
KRREIGHGRLARRGVQAMLPDSDRFPYVIRVVSEITESNGSSSMASVCGASLALMDAGVPIKAPVAGIAMGLVKEGERFA
VLSDILGDEDHLGDMDFKVAGSKDGITALQMDIKIEGITPDIMEQALKQAHAGRIHILDAMNKVLPESRTEINAHAPNYA
VIEINPDKIRDVIGKGGATIRQLTEETGAVIDIDDAGTIRIFGENKAATKAAIAKIEAITAEVEVGKTYEGTVARIVDFG
AFINVLPNTDGLVHISQIADERVENVSDYLKEGQIVKVLVQDVDNRGRIKLTMKGIEQS

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction [H]

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Homo sapiens, GI188528628, Length=713, Percent_Identity=37.1669004207574, Blast_Score=417, Evalue=1e-116,
Organism=Escherichia coli, GI145693187, Length=700, Percent_Identity=61.5714285714286, Blast_Score=853, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115534063, Length=669, Percent_Identity=33.3333333333333, Blast_Score=322, Evalue=3e-88,
Organism=Caenorhabditis elegans, GI17535281, Length=79, Percent_Identity=49.3670886075949, Blast_Score=72, Evalue=9e-13,
Organism=Saccharomyces cerevisiae, GI6320850, Length=105, Percent_Identity=35.2380952380952, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI281362905, Length=697, Percent_Identity=35.8680057388809, Blast_Score=417, Evalue=1e-116,
Organism=Drosophila melanogaster, GI24651641, Length=697, Percent_Identity=35.8680057388809, Blast_Score=417, Evalue=1e-116,
Organism=Drosophila melanogaster, GI24651643, Length=697, Percent_Identity=35.8680057388809, Blast_Score=417, Evalue=1e-116,
Organism=Drosophila melanogaster, GI161079377, Length=642, Percent_Identity=35.3582554517134, Blast_Score=380, Evalue=1e-105,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967 [H]

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1 [H]

EC number: =2.7.7.8 [H]

Molecular weight: Translated: 75683; Mature: 75552

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMFNTIKREFQYGNQQVVIETGRIARQANSILVHMGGVTVLVAAVVKSDAKEGQNFFPL
CCHHHHHHHHHHCCCCEEEEECCHHHHCCCCEEEEECHHHHHHHHHHHHHHHCCCCEEEE
TVNYQEKMYAAGKIPGAYGKREGRASESETLTSRLIDRPIRPLFPEGYVNEIQITATVVS
EEEHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEEE
SDKTQSADIAALIGASAALAISDAPFNGPVAAARVGFINGEYVLNPTLEELKESDLDLVV
CCCCCCCCHHHHHCCCEEEEEECCCCCCCCHHHEEEEECCCEEECCCHHHHHCCCCCEEE
AGTKSAVLMVESEAAELSEDQMLGAVLYGHQQQQIVIDNIASMAAEIGTAKQQYTAPVRN
ECCCCEEEEEECCHHHCCHHHHHHHHHHCCCCCEEHHHHHHHHHHHHCCCHHHHCCCHHH
QTLETGMKEQFGTQVSDAYTITDKQARYSKLDEIKDAALAALAGDAESESYADTVSELKE
HHHHHCHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
IYNDLKYRTVRDNILSGKPRIDGRDLETVRALDVQVGVLPFTHGSALFTRGETQALVTTT
HHHHHHHHHHHHHHHCCCCCCCCCCCHHHEEEEEEEEEEEECCCCEEEECCCCCEEEEEE
LGNTRDVNMIDSLAGTIRDHFMLHYNFPHFSVGETGREGIPKRREIGHGRLARRGVQAML
CCCCCCCHHHHHHHHHHHHCEEEEECCCCCCCCCCCCCCCCCHHHCCCCHHHHHCHHHCC
PDSDRFPYVIRVVSEITESNGSSSMASVCGASLALMDAGVPIKAPVAGIAMGLVKEGERF
CCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHEEECCCCCCCCHHHHHHHHHHCCCCE
AVLSDILGDEDHLGDMDFKVAGSKDGITALQMDIKIEGITPDIMEQALKQAHAGRIHILD
EHHHHHCCCCCCCCCCCEEEECCCCCCEEEEEEEEEECCCHHHHHHHHHHHCCCEEEEHH
AMNKVLPESRTEINAHAPNYAVIEINPDKIRDVIGKGGATIRQLTEETGAVIDIDDAGTI
HHHHHCCCCCCEEECCCCCEEEEEECHHHHHHHHCCCCHHHHHHHHHCCCEEEECCCCEE
RIFGENKAATKAAIAKIEAITAEVEVGKTYEGTVARIVDFGAFINVLPNTDGLVHISQIA
EEEECCCHHHHHHHHHHHHHEEEEEECCCCCCHHHHHHHHHHHHEECCCCCCEEEHHHHH
DERVENVSDYLKEGQIVKVLVQDVDNRGRIKLTMKGIEQS
HHHHHHHHHHHHCCCEEEEHHHCCCCCCEEEEEECCCCCC
>Mature Secondary Structure 
TMFNTIKREFQYGNQQVVIETGRIARQANSILVHMGGVTVLVAAVVKSDAKEGQNFFPL
CHHHHHHHHHHCCCCEEEEECCHHHHCCCCEEEEECHHHHHHHHHHHHHHHCCCCEEEE
TVNYQEKMYAAGKIPGAYGKREGRASESETLTSRLIDRPIRPLFPEGYVNEIQITATVVS
EEEHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEEE
SDKTQSADIAALIGASAALAISDAPFNGPVAAARVGFINGEYVLNPTLEELKESDLDLVV
CCCCCCCCHHHHHCCCEEEEEECCCCCCCCHHHEEEEECCCEEECCCHHHHHCCCCCEEE
AGTKSAVLMVESEAAELSEDQMLGAVLYGHQQQQIVIDNIASMAAEIGTAKQQYTAPVRN
ECCCCEEEEEECCHHHCCHHHHHHHHHHCCCCCEEHHHHHHHHHHHHCCCHHHHCCCHHH
QTLETGMKEQFGTQVSDAYTITDKQARYSKLDEIKDAALAALAGDAESESYADTVSELKE
HHHHHCHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
IYNDLKYRTVRDNILSGKPRIDGRDLETVRALDVQVGVLPFTHGSALFTRGETQALVTTT
HHHHHHHHHHHHHHHCCCCCCCCCCCHHHEEEEEEEEEEEECCCCEEEECCCCCEEEEEE
LGNTRDVNMIDSLAGTIRDHFMLHYNFPHFSVGETGREGIPKRREIGHGRLARRGVQAML
CCCCCCCHHHHHHHHHHHHCEEEEECCCCCCCCCCCCCCCCCHHHCCCCHHHHHCHHHCC
PDSDRFPYVIRVVSEITESNGSSSMASVCGASLALMDAGVPIKAPVAGIAMGLVKEGERF
CCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHEEECCCCCCCCHHHHHHHHHHCCCCE
AVLSDILGDEDHLGDMDFKVAGSKDGITALQMDIKIEGITPDIMEQALKQAHAGRIHILD
EHHHHHCCCCCCCCCCCEEEECCCCCCEEEEEEEEEECCCHHHHHHHHHHHCCCEEEEHH
AMNKVLPESRTEINAHAPNYAVIEINPDKIRDVIGKGGATIRQLTEETGAVIDIDDAGTI
HHHHHCCCCCCEEECCCCCEEEEEECHHHHHHHHCCCCHHHHHHHHHCCCEEEECCCCEE
RIFGENKAATKAAIAKIEAITAEVEVGKTYEGTVARIVDFGAFINVLPNTDGLVHISQIA
EEEECCCHHHHHHHHHHHHHEEEEEECCCCCCHHHHHHHHHHHHEECCCCCCEEEHHHHH
DERVENVSDYLKEGQIVKVLVQDVDNRGRIKLTMKGIEQS
HHHHHHHHHHHHCCCEEEEHHHCCCCCCEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA