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The map label for this gene is dnaN [H]

Identifier: 93004835

GI number: 93004835

Start: 2971

End: 4146

Strand: Direct

Name: dnaN [H]

Synonym: Pcryo_0004

Alternate gene names: 93004835

Gene position: 2971-4146 (Clockwise)

Preceding gene: 93004834

Following gene: 93004836

Centisome position: 0.1

GC content: 42.43

Gene sequence:

>1176_bases
ATGCAATTATCGATTAATCGAGAGTCGTTACTAAAAGCCATTAACTTGATCGCCAAGGCTGCTGATAAACGCCATAATAT
GGTTATTTTGGGTAATATCAAGCTACAGCTATTTGAATCAGAGCTTGTTTTGACCGCTTCTGATTTAGAAGTAGAGCTGA
CATCGACGTTAAAATTGCCTGCTGGAGCTTGTGTTGAAGCAGGTACGACGACATTGCCTGCCACTAAATTAAGAGATATC
TGTAAATCTTTGCCTGCTCAAGCACAAGTCAGTCTGATAACTCAAGAAAACCAACGCTGCTTATTGACCAGTGGCAAAAG
TCGTTTTACCTTAGGTACATTGCCTAGCGAAGACTATCCAAGTTTGGGTAACCCTGAAAATATCACGCCATTGACGATTA
GCCGTAGTCGTCTTATTGATTTGATTCATAAGACCCAATTTGCGATGGCGATTCAAGATGTCCGTTATTATCTAACGGGT
ATGCTATTTGATGTATCACAGCAGCAGTTAACGACGGTTGCAACCGATGGTCATAGACTGGCATTAGCACGTAGTGTTAT
TGAAGTCAGTGCTGATTTAAGTATGCAGGCTATCTTGCCACGTAAGGCTGTAATTGAGCTTGAGCGTTTGGCTACCGAGC
TGGGCAAAATGCTTGGTGATAAAGACAATGCCGTCACGTTGAGTTTCGGTCGTGAATTTTTGCAAGTGAGCTTACCATTT
GGTGACGTTGATAGTGCAGGGCAGGTTAGTCAGGATTTGATGGTGACTTTTACCGCGCGTCTGATTGATGGTAAGTTTCC
TGATTATCGCCGTGTGATGCCTAGCAATACAGACAAACTTGCTCTATTTAATCAAGAGAAAATGATGGATGTGCTGCGCC
GTGTCGCGATTTTGAGTAATGAGAAGTCTCGTGGCGTAGTATTTAACTTTGCCAGTGATGGTATGGTAGAAGTACGTGCC
AATAACGCTGAGCAAGATGAAGCGGTTGAGATGATTCAAGCTAAGTATCAAGGCGAGCCAATGGAGTTGTCTTTTAATGC
TGCTTATTTACAAGACGTGCTAGGTGTCATCCAAGGCGACTTGCAAATGCATATGAGCCAGTCAAATGCGTCAGTATTGG
TTAATCAGTTAAATGATGAGTTTCATCAGTACGTTATTATGCCAATGCGCATATAA

Upstream 100 bases:

>100_bases
GTATACAGACCCCTAATAGATTATCGTAAGTAGACAGGGTATATTAAGTCAATATTCTTTATTTTTACAAAAAGAATCAA
TCAAATAAGAGTAACTAAGC

Downstream 100 bases:

>100_bases
TCTATTCTTTAAATGATAGATGATTAGGGTTGAAAATTATAATTGTTATGGATTTTAACCCTAATCATATCGATAATTTT
AAGCTTCCAAAGCTTAAAAT

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 391; Mature: 391

Protein sequence:

>391_residues
MQLSINRESLLKAINLIAKAADKRHNMVILGNIKLQLFESELVLTASDLEVELTSTLKLPAGACVEAGTTTLPATKLRDI
CKSLPAQAQVSLITQENQRCLLTSGKSRFTLGTLPSEDYPSLGNPENITPLTISRSRLIDLIHKTQFAMAIQDVRYYLTG
MLFDVSQQQLTTVATDGHRLALARSVIEVSADLSMQAILPRKAVIELERLATELGKMLGDKDNAVTLSFGREFLQVSLPF
GDVDSAGQVSQDLMVTFTARLIDGKFPDYRRVMPSNTDKLALFNQEKMMDVLRRVAILSNEKSRGVVFNFASDGMVEVRA
NNAEQDEAVEMIQAKYQGEPMELSFNAAYLQDVLGVIQGDLQMHMSQSNASVLVNQLNDEFHQYVIMPMRI

Sequences:

>Translated_391_residues
MQLSINRESLLKAINLIAKAADKRHNMVILGNIKLQLFESELVLTASDLEVELTSTLKLPAGACVEAGTTTLPATKLRDI
CKSLPAQAQVSLITQENQRCLLTSGKSRFTLGTLPSEDYPSLGNPENITPLTISRSRLIDLIHKTQFAMAIQDVRYYLTG
MLFDVSQQQLTTVATDGHRLALARSVIEVSADLSMQAILPRKAVIELERLATELGKMLGDKDNAVTLSFGREFLQVSLPF
GDVDSAGQVSQDLMVTFTARLIDGKFPDYRRVMPSNTDKLALFNQEKMMDVLRRVAILSNEKSRGVVFNFASDGMVEVRA
NNAEQDEAVEMIQAKYQGEPMELSFNAAYLQDVLGVIQGDLQMHMSQSNASVLVNQLNDEFHQYVIMPMRI
>Mature_391_residues
MQLSINRESLLKAINLIAKAADKRHNMVILGNIKLQLFESELVLTASDLEVELTSTLKLPAGACVEAGTTTLPATKLRDI
CKSLPAQAQVSLITQENQRCLLTSGKSRFTLGTLPSEDYPSLGNPENITPLTISRSRLIDLIHKTQFAMAIQDVRYYLTG
MLFDVSQQQLTTVATDGHRLALARSVIEVSADLSMQAILPRKAVIELERLATELGKMLGDKDNAVTLSFGREFLQVSLPF
GDVDSAGQVSQDLMVTFTARLIDGKFPDYRRVMPSNTDKLALFNQEKMMDVLRRVAILSNEKSRGVVFNFASDGMVEVRA
NNAEQDEAVEMIQAKYQGEPMELSFNAAYLQDVLGVIQGDLQMHMSQSNASVLVNQLNDEFHQYVIMPMRI

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=392, Percent_Identity=39.2857142857143, Blast_Score=264, Evalue=8e-72,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 43345; Mature: 43345

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLSINRESLLKAINLIAKAADKRHNMVILGNIKLQLFESELVLTASDLEVELTSTLKLP
CEECCCHHHHHHHHHHHHHHHCCCCCEEEEECEEEEEEECEEEEEECCCEEEEEEEECCC
AGACVEAGTTTLPATKLRDICKSLPAQAQVSLITQENQRCLLTSGKSRFTLGTLPSEDYP
CCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEEECCCCEEEEECCCCEEEEECCCCCCCC
SLGNPENITPLTISRSRLIDLIHKTQFAMAIQDVRYYLTGMLFDVSQQQLTTVATDGHRL
CCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHEEECCCCHH
ALARSVIEVSADLSMQAILPRKAVIELERLATELGKMLGDKDNAVTLSFGREFLQVSLPF
HHHHHHHHHHCCCCHHHHCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCEEEEEECCC
GDVDSAGQVSQDLMVTFTARLIDGKFPDYRRVMPSNTDKLALFNQEKMMDVLRRVAILSN
CCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCEEEEECHHHHHHHHHHHHHHCC
EKSRGVVFNFASDGMVEVRANNAEQDEAVEMIQAKYQGEPMELSFNAAYLQDVLGVIQGD
CCCCCEEEEECCCCEEEEECCCCCHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHH
LQMHMSQSNASVLVNQLNDEFHQYVIMPMRI
HHHEECCCCHHHHHHHHHHHHHHEEEEEECC
>Mature Secondary Structure
MQLSINRESLLKAINLIAKAADKRHNMVILGNIKLQLFESELVLTASDLEVELTSTLKLP
CEECCCHHHHHHHHHHHHHHHCCCCCEEEEECEEEEEEECEEEEEECCCEEEEEEEECCC
AGACVEAGTTTLPATKLRDICKSLPAQAQVSLITQENQRCLLTSGKSRFTLGTLPSEDYP
CCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEEECCCCEEEEECCCCEEEEECCCCCCCC
SLGNPENITPLTISRSRLIDLIHKTQFAMAIQDVRYYLTGMLFDVSQQQLTTVATDGHRL
CCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHEEECCCCHH
ALARSVIEVSADLSMQAILPRKAVIELERLATELGKMLGDKDNAVTLSFGREFLQVSLPF
HHHHHHHHHHCCCCHHHHCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCEEEEEECCC
GDVDSAGQVSQDLMVTFTARLIDGKFPDYRRVMPSNTDKLALFNQEKMMDVLRRVAILSN
CCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCEEEEECHHHHHHHHHHHHHHCC
EKSRGVVFNFASDGMVEVRANNAEQDEAVEMIQAKYQGEPMELSFNAAYLQDVLGVIQGD
CCCCCEEEEECCCCEEEEECCCCCHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHH
LQMHMSQSNASVLVNQLNDEFHQYVIMPMRI
HHHEECCCCHHHHHHHHHHHHHHEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]