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The map label for this gene is dnaA [H]

Identifier: 93004834

GI number: 93004834

Start: 1365

End: 2810

Strand: Direct

Name: dnaA [H]

Synonym: Pcryo_0003

Alternate gene names: 93004834

Gene position: 1365-2810 (Clockwise)

Preceding gene: NA

Following gene: 93004835

Centisome position: 0.04

GC content: 38.04

Gene sequence:

>1446_bases
ATGATAGATACAGCAAATATTTGGGATAAATGCCTAAACGACTTACGTTATCATGTCAAAGATAACGTGTTTACCATGTG
GTTAAGACCATTGTCAGCTCATCAAGAAAACAACACTTTAATCATTCTTGCACCTAATGATTACTTTGTAACTTACATCA
AAAAGAACCACTTAGAGGATATTCAGCAGCTGGTTGCTAAGCATAGTCAGGGAAGTATTGAAGAAGTTATTGTACGTGTT
GATAATGCTGGTCGCGATATCGATGATAGTAGCTCGCTGCAATCGGGATCATTATTCTCAGAAGATAACCTCTCCCCTAC
CCCTTCAAATCATGATTTTGAAACGATTCATCATAATAATGCTAAAGCAGATATCGATGCCAATATGCGTCATGCGGAAG
CCATTGACTCTGCTGATGCGAAATCCTTAAATTATTTAAATCCTGATTTTACCTTTGAAACCTTTGTCACAGGTAAGTCT
AACAATCTTGCTTATAAGGCGTGCTACGAGCTTGGTAAACGTCAATCAAAAAATCGTCACAATCCTTTGTTTATATATGG
ACCTTCTGGTTTGGGGAAAACCCATTTAATGCATTCAGTAGCACATCGCTATTTAAAAAACAATCAAAATTTTTATTATT
TCACTTCTGAAAAATTTATTAATCAATTGGTTTATTCATTAAGAAATAATAAAATAGAAGATTTTAAAAAGAAAATAAAA
AAAGTAGATTTATTAATAGTAGATGATATTCATGTATTAGCTGGAAAGACTAAAAGTAGTAATGAGTTTTTAACATTGTT
TGCAGATTTTACTAGTGGTGATAAACAGCTGATTTTGGCATCTGATCGGCATCCATCACAAATGACTGAATTTGACGAGC
GCTTTAGATCTCGTTTTTCTTGGGGCTTAACGGTCGCAGTTGATCCGCCTGAAATTGATACGCGCGTACAAATCTTACAA
AGGAAAGCCGCCTCTTATGGAATGAGCCTTCCTAAAGAATGTGCGTTATTCATCGCTCAAAATGTTGTTTCTAATGTGAG
ACGTTTAGAAGGTGCTTTAAACCAGGTATTTGCCAATGCTAACTTAACCGGCGCCCCTATTACTCTTGAAATGGTGCAAT
ATGCCTTAAAAGATATTGTTGCCATGCGCGTACAAGCCGTCAATATGGATAATATTCGTAAAGTGGTCGCTGAATATTAT
GATGTGACGATTAAGGATATGCTCGGTCGCAAGCGTACGCGTAGTATTGCTAGACCTCGCCAAATAGCGATGGCACTATC
ACGAGAGTTGACCAGCGAGAGTTTTCCTGAGATTGGGCAGTCTTTTGGAGGACGCGACCACACAACAGTGATGCATGCTT
GTGATAAAGTGAATGAATTACGTGCCGCCGATCCAGCGTTTGACAAGGATTACAAGACCCTAGCTTTGATGCTGCAAGCT
GGTTAG

Upstream 100 bases:

>100_bases
AGGTTGTTTAACGTAAACCTCAACGCCTTTTTAAACCTAATTCGATCCATTCTTACAATAAAAAATGCTAATACTTTGAA
CTATAGGGCTACTGATATTC

Downstream 100 bases:

>100_bases
AAGGCTCTACTATTACTATATTTTAGTAGATAGATATGGTTTATAATGTTGAAAACGGGCGTATACAGACCCCTAATAGA
TTATCGTAAGTAGACAGGGT

Product: chromosomal replication initiator protein DnaA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 481; Mature: 481

Protein sequence:

>481_residues
MIDTANIWDKCLNDLRYHVKDNVFTMWLRPLSAHQENNTLIILAPNDYFVTYIKKNHLEDIQQLVAKHSQGSIEEVIVRV
DNAGRDIDDSSSLQSGSLFSEDNLSPTPSNHDFETIHHNNAKADIDANMRHAEAIDSADAKSLNYLNPDFTFETFVTGKS
NNLAYKACYELGKRQSKNRHNPLFIYGPSGLGKTHLMHSVAHRYLKNNQNFYYFTSEKFINQLVYSLRNNKIEDFKKKIK
KVDLLIVDDIHVLAGKTKSSNEFLTLFADFTSGDKQLILASDRHPSQMTEFDERFRSRFSWGLTVAVDPPEIDTRVQILQ
RKAASYGMSLPKECALFIAQNVVSNVRRLEGALNQVFANANLTGAPITLEMVQYALKDIVAMRVQAVNMDNIRKVVAEYY
DVTIKDMLGRKRTRSIARPRQIAMALSRELTSESFPEIGQSFGGRDHTTVMHACDKVNELRAADPAFDKDYKTLALMLQA
G

Sequences:

>Translated_481_residues
MIDTANIWDKCLNDLRYHVKDNVFTMWLRPLSAHQENNTLIILAPNDYFVTYIKKNHLEDIQQLVAKHSQGSIEEVIVRV
DNAGRDIDDSSSLQSGSLFSEDNLSPTPSNHDFETIHHNNAKADIDANMRHAEAIDSADAKSLNYLNPDFTFETFVTGKS
NNLAYKACYELGKRQSKNRHNPLFIYGPSGLGKTHLMHSVAHRYLKNNQNFYYFTSEKFINQLVYSLRNNKIEDFKKKIK
KVDLLIVDDIHVLAGKTKSSNEFLTLFADFTSGDKQLILASDRHPSQMTEFDERFRSRFSWGLTVAVDPPEIDTRVQILQ
RKAASYGMSLPKECALFIAQNVVSNVRRLEGALNQVFANANLTGAPITLEMVQYALKDIVAMRVQAVNMDNIRKVVAEYY
DVTIKDMLGRKRTRSIARPRQIAMALSRELTSESFPEIGQSFGGRDHTTVMHACDKVNELRAADPAFDKDYKTLALMLQA
G
>Mature_481_residues
MIDTANIWDKCLNDLRYHVKDNVFTMWLRPLSAHQENNTLIILAPNDYFVTYIKKNHLEDIQQLVAKHSQGSIEEVIVRV
DNAGRDIDDSSSLQSGSLFSEDNLSPTPSNHDFETIHHNNAKADIDANMRHAEAIDSADAKSLNYLNPDFTFETFVTGKS
NNLAYKACYELGKRQSKNRHNPLFIYGPSGLGKTHLMHSVAHRYLKNNQNFYYFTSEKFINQLVYSLRNNKIEDFKKKIK
KVDLLIVDDIHVLAGKTKSSNEFLTLFADFTSGDKQLILASDRHPSQMTEFDERFRSRFSWGLTVAVDPPEIDTRVQILQ
RKAASYGMSLPKECALFIAQNVVSNVRRLEGALNQVFANANLTGAPITLEMVQYALKDIVAMRVQAVNMDNIRKVVAEYY
DVTIKDMLGRKRTRSIARPRQIAMALSRELTSESFPEIGQSFGGRDHTTVMHACDKVNELRAADPAFDKDYKTLALMLQA
G

Specific function: Plays an important role in the initiation and regulation of chromosomal replication. Binds to the origin of replication; it binds specifically double-stranded DNA at a 9 bp consensus (dnaA box):5'-TTATC[CA]A[CA]A-3'. DnaA binds to ATP and to acidic phosph

COG id: COG0593

COG function: function code L; ATPase involved in DNA replication initiation

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dnaA family [H]

Homologues:

Organism=Escherichia coli, GI2367267, Length=472, Percent_Identity=39.8305084745763, Blast_Score=368, Evalue=1e-103,
Organism=Escherichia coli, GI226510964, Length=234, Percent_Identity=22.6495726495727, Blast_Score=65, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR001957
- InterPro:   IPR020591
- InterPro:   IPR018312
- InterPro:   IPR013159
- InterPro:   IPR013317
- InterPro:   IPR010921 [H]

Pfam domain/function: PF00308 Bac_DnaA; PF08299 Bac_DnaA_C [H]

EC number: NA

Molecular weight: Translated: 54639; Mature: 54639

Theoretical pI: Translated: 7.76; Mature: 7.76

Prosite motif: PS01008 DNAA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDTANIWDKCLNDLRYHVKDNVFTMWLRPLSAHQENNTLIILAPNDYFVTYIKKNHLED
CCCCHHHHHHHHHHHHHHHCCCCCEEEHHHHHCCCCCCEEEEEECCCEEEEEEHHHHHHH
IQQLVAKHSQGSIEEVIVRVDNAGRDIDDSSSLQSGSLFSEDNLSPTPSNHDFETIHHNN
HHHHHHHCCCCCHHHHHEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHEECCC
AKADIDANMRHAEAIDSADAKSLNYLNPDFTFETFVTGKSNNLAYKACYELGKRQSKNRH
CCCCCCCCCHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCC
NPLFIYGPSGLGKTHLMHSVAHRYLKNNQNFYYFTSEKFINQLVYSLRNNKIEDFKKKIK
CCEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHCCCHHHHHHHHH
KVDLLIVDDIHVLAGKTKSSNEFLTLFADFTSGDKQLILASDRHPSQMTEFDERFRSRFS
HHCEEEEECHHEEECCCCCCCCEEEEEEECCCCCCEEEEECCCCCHHHHHHHHHHHHHHC
WGLTVAVDPPEIDTRVQILQRKAASYGMSLPKECALFIAQNVVSNVRRLEGALNQVFANA
CCEEEEECCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NLTGAPITLEMVQYALKDIVAMRVQAVNMDNIRKVVAEYYDVTIKDMLGRKRTRSIARPR
CCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHCHHHHHHHCCHH
QIAMALSRELTSESFPEIGQSFGGRDHTTVMHACDKVNELRAADPAFDKDYKTLALMLQA
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHEEEEEEEC
G
C
>Mature Secondary Structure
MIDTANIWDKCLNDLRYHVKDNVFTMWLRPLSAHQENNTLIILAPNDYFVTYIKKNHLED
CCCCHHHHHHHHHHHHHHHCCCCCEEEHHHHHCCCCCCEEEEEECCCEEEEEEHHHHHHH
IQQLVAKHSQGSIEEVIVRVDNAGRDIDDSSSLQSGSLFSEDNLSPTPSNHDFETIHHNN
HHHHHHHCCCCCHHHHHEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHEECCC
AKADIDANMRHAEAIDSADAKSLNYLNPDFTFETFVTGKSNNLAYKACYELGKRQSKNRH
CCCCCCCCCHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCC
NPLFIYGPSGLGKTHLMHSVAHRYLKNNQNFYYFTSEKFINQLVYSLRNNKIEDFKKKIK
CCEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHCCCHHHHHHHHH
KVDLLIVDDIHVLAGKTKSSNEFLTLFADFTSGDKQLILASDRHPSQMTEFDERFRSRFS
HHCEEEEECHHEEECCCCCCCCEEEEEEECCCCCCEEEEECCCCCHHHHHHHHHHHHHHC
WGLTVAVDPPEIDTRVQILQRKAASYGMSLPKECALFIAQNVVSNVRRLEGALNQVFANA
CCEEEEECCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NLTGAPITLEMVQYALKDIVAMRVQAVNMDNIRKVVAEYYDVTIKDMLGRKRTRSIARPR
CCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHCHHHHHHHCCHH
QIAMALSRELTSESFPEIGQSFGGRDHTTVMHACDKVNELRAADPAFDKDYKTLALMLQA
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHEEEEEEEC
G
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA