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The map label for this gene is fumB [H]

Identifier: 92113957

GI number: 92113957

Start: 2090105

End: 2091610

Strand: Direct

Name: fumB [H]

Synonym: Csal_1834

Alternate gene names: 92113957

Gene position: 2090105-2091610 (Clockwise)

Preceding gene: 92113956

Following gene: 92113958

Centisome position: 56.54

GC content: 62.68

Gene sequence:

>1506_bases
ATGACCGTGATACGCCAGGACGATGTCATCCAGAGCGTGGCCGATGCCTTGCAGTTCATCTCTTATTACCACCCCAAAGA
TTTCATCGATGCCATGCACGAGGCGTATCAGCGTGAAGAAAACCCCGCTGCACGTGATGCCATCGCACAGATTCTGATCA
ATTCACGCATGTGTGCCGAGGGACATCGCCCCATCTGTCAGGACACCGGCATCGTGACCGTTTTCGTCGAGGTCGGGATG
AATGTCCGCTGGGACGCGAGCATGAGCCTCGACGACATGATCAACGAGGGGGTACGGCAGGCATACCAGCATCCAGACAA
CGTCTTGCGCGCGTCGGTACTCGCCGATCCCGATGGCAAGCGCACCAATACGCGCGACAACACGCCGGCCGTCATTCACC
ACAAGCTGGTTCCCGGCGACAAGGTCGAGATTCATGTCGCGGCCAAAGGGGGCGGCAGCGAAGCCAAGTCCAAGTTTGCC
ATGCTCAACCCATCCGACAACGTCGCCGAGTGGGTGCTCGAACAGCTGCCCAAGATGGGCGCGGGCTGGTGCCCACCGGG
CATGCTGGGTATCGGGATCGGCGGTACCGCCGAGAAAGCCATGGAACTCGCCAAGGAGTCCCTGCTCGACCCCATCGACA
TCCAGGCACTCAAGGCGAGGGGAGCCGAAACACGGGCCGAGGAGCTGCGCCTCGAACTCTTCGACCAGGTCAATCGCACG
GGTATCGGCGCACAGGGCCTGGGTGGACTGACCACCGTGCTCGATATCAAGGTCAAGGATTATCCGACCCACGCGGCCAA
CAAGCCCGTGGCCATCATTCCCAATTGCGCGGCGACACGCCACGTCCACTTTATGCTGGACGGCAGTGGCACCGCATCGC
TGCCCATCCCGACGCTCGACGACTGGCCGCAAATCACCCGCGAGGTCGGGGACAACGTCAAGCGCGTCAACCTCGACACG
GTGACCGCCGAGGAGGCCGCTTCCTGGCAGCCGGGCGACACGCTGCTGCTCAACGGCAAGCTCCTGACCGGCCGCGACGC
CGCACACAAGCGCATGGTCGACATGCTCTCCAAAGGAGAAGACTTGCCGGTCGATCTGCGCGGGCGCTTCATCTATTACG
TAGGCCCGGTGGATCCGGTCGGCGATGAAGTCGTAGGCCCCGCCGGCCCCACGACGGCTACGCGCATGGACAAGTTCACG
CGGACCATGCTGGAACAGACCGGCCTGCAAGGCATGGTCGGAAAGGCCGAGCGCGGTCCCATGGCGATCGAAGCCATTCG
CGACAATCGCGCCGTGTATCTCATGGCGGTCGGGGGCGCGGCCTATCTCGTCGCCCAGGCGATCAAGAAATCCCGCGTGG
TAGCCTTCGAAGATCTGGGCATGGAAGCGATCTACGAGTTCGACGTGGAAGACATGCCCGTCACCGTGGCCGTGGACAGT
CGCGGAGAGTCCGTTCATCAGACGGGGCCCGCCAAGTGGAAAGAGATCATCGCCGCGAGCGCCTGA

Upstream 100 bases:

>100_bases
GTGATACGACATTTAGGTATAATCCCGCGCTCATGAAAGCTCGCGTCGAGATGCCCTGACCATTCGCCGCGAGATGTTTT
CCCCAAGGAGTCTTTCTCTC

Downstream 100 bases:

>100_bases
AGCGTGAGATGATCACTCGTGCCAGGCCCCGCACTCGCGGGGCCTGATTTTTAGCCGCCGAGCGATTGCGAGGGGCCAGG
GAGGCGCCATCATGACATCC

Product: fumarase

Products: NA

Alternate protein names: Fumarase [H]

Number of amino acids: Translated: 501; Mature: 500

Protein sequence:

>501_residues
MTVIRQDDVIQSVADALQFISYYHPKDFIDAMHEAYQREENPAARDAIAQILINSRMCAEGHRPICQDTGIVTVFVEVGM
NVRWDASMSLDDMINEGVRQAYQHPDNVLRASVLADPDGKRTNTRDNTPAVIHHKLVPGDKVEIHVAAKGGGSEAKSKFA
MLNPSDNVAEWVLEQLPKMGAGWCPPGMLGIGIGGTAEKAMELAKESLLDPIDIQALKARGAETRAEELRLELFDQVNRT
GIGAQGLGGLTTVLDIKVKDYPTHAANKPVAIIPNCAATRHVHFMLDGSGTASLPIPTLDDWPQITREVGDNVKRVNLDT
VTAEEAASWQPGDTLLLNGKLLTGRDAAHKRMVDMLSKGEDLPVDLRGRFIYYVGPVDPVGDEVVGPAGPTTATRMDKFT
RTMLEQTGLQGMVGKAERGPMAIEAIRDNRAVYLMAVGGAAYLVAQAIKKSRVVAFEDLGMEAIYEFDVEDMPVTVAVDS
RGESVHQTGPAKWKEIIAASA

Sequences:

>Translated_501_residues
MTVIRQDDVIQSVADALQFISYYHPKDFIDAMHEAYQREENPAARDAIAQILINSRMCAEGHRPICQDTGIVTVFVEVGM
NVRWDASMSLDDMINEGVRQAYQHPDNVLRASVLADPDGKRTNTRDNTPAVIHHKLVPGDKVEIHVAAKGGGSEAKSKFA
MLNPSDNVAEWVLEQLPKMGAGWCPPGMLGIGIGGTAEKAMELAKESLLDPIDIQALKARGAETRAEELRLELFDQVNRT
GIGAQGLGGLTTVLDIKVKDYPTHAANKPVAIIPNCAATRHVHFMLDGSGTASLPIPTLDDWPQITREVGDNVKRVNLDT
VTAEEAASWQPGDTLLLNGKLLTGRDAAHKRMVDMLSKGEDLPVDLRGRFIYYVGPVDPVGDEVVGPAGPTTATRMDKFT
RTMLEQTGLQGMVGKAERGPMAIEAIRDNRAVYLMAVGGAAYLVAQAIKKSRVVAFEDLGMEAIYEFDVEDMPVTVAVDS
RGESVHQTGPAKWKEIIAASA
>Mature_500_residues
TVIRQDDVIQSVADALQFISYYHPKDFIDAMHEAYQREENPAARDAIAQILINSRMCAEGHRPICQDTGIVTVFVEVGMN
VRWDASMSLDDMINEGVRQAYQHPDNVLRASVLADPDGKRTNTRDNTPAVIHHKLVPGDKVEIHVAAKGGGSEAKSKFAM
LNPSDNVAEWVLEQLPKMGAGWCPPGMLGIGIGGTAEKAMELAKESLLDPIDIQALKARGAETRAEELRLELFDQVNRTG
IGAQGLGGLTTVLDIKVKDYPTHAANKPVAIIPNCAATRHVHFMLDGSGTASLPIPTLDDWPQITREVGDNVKRVNLDTV
TAEEAASWQPGDTLLLNGKLLTGRDAAHKRMVDMLSKGEDLPVDLRGRFIYYVGPVDPVGDEVVGPAGPTTATRMDKFTR
TMLEQTGLQGMVGKAERGPMAIEAIRDNRAVYLMAVGGAAYLVAQAIKKSRVVAFEDLGMEAIYEFDVEDMPVTVAVDSR
GESVHQTGPAKWKEIIAASA

Specific function: It functions in the generation of fumarate for use as an anaerobic electron acceptor [H]

COG id: COG1951

COG function: function code C; Tartrate dehydratase alpha subunit/Fumarate hydratase class I, N-terminal domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I fumarase family [H]

Homologues:

Organism=Escherichia coli, GI1790564, Length=496, Percent_Identity=30.8467741935484, Blast_Score=195, Evalue=6e-51,
Organism=Escherichia coli, GI1787897, Length=458, Percent_Identity=33.1877729257642, Blast_Score=190, Evalue=2e-49,
Organism=Escherichia coli, GI1789442, Length=277, Percent_Identity=27.7978339350181, Blast_Score=99, Evalue=8e-22,
Organism=Escherichia coli, GI1789443, Length=178, Percent_Identity=32.5842696629214, Blast_Score=99, Evalue=9e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004646
- InterPro:   IPR004647
- InterPro:   IPR011167
- InterPro:   IPR020557 [H]

Pfam domain/function: PF05681 Fumerase; PF05683 Fumerase_C [H]

EC number: =4.2.1.2 [H]

Molecular weight: Translated: 54350; Mature: 54218

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVIRQDDVIQSVADALQFISYYHPKDFIDAMHEAYQREENPAARDAIAQILINSRMCAE
CCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHCC
GHRPICQDTGIVTVFVEVGMNVRWDASMSLDDMINEGVRQAYQHPDNVLRASVLADPDGK
CCCCCCCCCCEEEEEEECCCCEEECCCCCHHHHHHHHHHHHHHCCHHHHHHHHEECCCCC
RTNTRDNTPAVIHHKLVPGDKVEIHVAAKGGGSEAKSKFAMLNPSDNVAEWVLEQLPKMG
CCCCCCCCCEEEEEECCCCCEEEEEEEECCCCCHHHCCEEEECCCCHHHHHHHHHHHHCC
AGWCPPGMLGIGIGGTAEKAMELAKESLLDPIDIQALKARGAETRAEELRLELFDQVNRT
CCCCCCCCEEECCCCHHHHHHHHHHHHCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHC
GIGAQGLGGLTTVLDIKVKDYPTHAANKPVAIIPNCAATRHVHFMLDGSGTASLPIPTLD
CCCCCCCCCEEEEEEEEEECCCCCCCCCCEEEECCCCCCEEEEEEEECCCCCCCCCCCCC
DWPQITREVGDNVKRVNLDTVTAEEAASWQPGDTLLLNGKLLTGRDAAHKRMVDMLSKGE
CHHHHHHHHCCCCEEEEECEEEHHHHCCCCCCCEEEEECEEEECCHHHHHHHHHHHHCCC
DLPVDLRGRFIYYVGPVDPVGDEVVGPAGPTTATRMDKFTRTMLEQTGLQGMVGKAERGP
CCCEEECCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
MAIEAIRDNRAVYLMAVGGAAYLVAQAIKKSRVVAFEDLGMEAIYEFDVEDMPVTVAVDS
EEEEEECCCCEEEEEEECCHHHHHHHHHHHCCEEEEHHCCCCEEEECCCCCCCEEEEECC
RGESVHQTGPAKWKEIIAASA
CCCCHHCCCCHHHHHHHHCCC
>Mature Secondary Structure 
TVIRQDDVIQSVADALQFISYYHPKDFIDAMHEAYQREENPAARDAIAQILINSRMCAE
CCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHCC
GHRPICQDTGIVTVFVEVGMNVRWDASMSLDDMINEGVRQAYQHPDNVLRASVLADPDGK
CCCCCCCCCCEEEEEEECCCCEEECCCCCHHHHHHHHHHHHHHCCHHHHHHHHEECCCCC
RTNTRDNTPAVIHHKLVPGDKVEIHVAAKGGGSEAKSKFAMLNPSDNVAEWVLEQLPKMG
CCCCCCCCCEEEEEECCCCCEEEEEEEECCCCCHHHCCEEEECCCCHHHHHHHHHHHHCC
AGWCPPGMLGIGIGGTAEKAMELAKESLLDPIDIQALKARGAETRAEELRLELFDQVNRT
CCCCCCCCEEECCCCHHHHHHHHHHHHCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHC
GIGAQGLGGLTTVLDIKVKDYPTHAANKPVAIIPNCAATRHVHFMLDGSGTASLPIPTLD
CCCCCCCCCEEEEEEEEEECCCCCCCCCCEEEECCCCCCEEEEEEEECCCCCCCCCCCCC
DWPQITREVGDNVKRVNLDTVTAEEAASWQPGDTLLLNGKLLTGRDAAHKRMVDMLSKGE
CHHHHHHHHCCCCEEEEECEEEHHHHCCCCCCCEEEEECEEEECCHHHHHHHHHHHHCCC
DLPVDLRGRFIYYVGPVDPVGDEVVGPAGPTTATRMDKFTRTMLEQTGLQGMVGKAERGP
CCCEEECCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
MAIEAIRDNRAVYLMAVGGAAYLVAQAIKKSRVVAFEDLGMEAIYEFDVEDMPVTVAVDS
EEEEEECCCCEEEEEEECCHHHHHHHHHHHCCEEEEHHCCCCEEEECCCCCCCEEEEECC
RGESVHQTGPAKWKEIIAASA
CCCCHHCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2656658; 7610040; 9278503 [H]