Click here to switch to the map view.

The map label for this gene is dacA [H]

Identifier: 92112285

GI number: 92112285

Start: 171412

End: 172653

Strand: Direct

Name: dacA [H]

Synonym: Csal_0150

Alternate gene names: 92112285

Gene position: 171412-172653 (Clockwise)

Preceding gene: 92112283

Following gene: 92112287

Centisome position: 4.64

GC content: 67.31

Gene sequence:

>1242_bases
ATGACGTCCCTGCATTCCCAGCGTGCGCGGCTTTTGCCCGCCCTGATCCTGTTGGTCGTTTTCGCCATCGCGTTGTGCAC
GCTGTCCGTGGCCGAAGCGGCCCCCAACCCGCGCTACGCGTCCATCGTCATCGATACCGAGAACGGCGCCGTCCTGCATG
CCGACAACGCCGATGCGCCTCGCTACCCGGCCTCGTTGACCAAGATGATGACGCTGTATCTGCTCTTCGAGGCGATCGAG
GACGATACGCTCAGCCTGGAGAGCCGCTTGCCGGTGTCGCAGTACGCCGCGGCGAAGCCGCCCTCCAAGATGGGCGTGAC
GCCCGGCGAGACGGTGAAGGTGGAGGACGCCATCAACATCCTCATCGTCAAGTCAGCCAATGACGTGGCGGCGGTGGTGG
CCGAAGGGCTGGCCGGCAGCGAGTCGGTCTTCGCCGGCCAGATGACCGACAAGGCGCGTGCTCTGGGCATGATGGACACG
ACCTTTCGCAATGCCTCGGGCCTGCCCGACGCGGGCCAGGTCACGACGGCGCGGGATATGGCCATTCTCGCGCTGCGGCT
GATGCAGGATTTCCCCGGCTATTACCCGTATTTCGGCAAGACCCAGTTCACCTGGCAGGGCAAGCGCTACCAGGGGCACA
ACCGGCTGCTGGATAGCTATGCGGGGGCCGATGGCCTCAAGACCGGTTACATTCGCGCCTCGGGCTTCAACGTGGCGACC
TCTGCCGTTCACGATGGGCGCCGCATCCTCTCGGTGGTGATGGGCGGCTTCACGGCGCAATCGCGCGATGCCCACATGCG
TGAACTGCTCGATAGCGGCTTCGCGCGTGTCGCATCGCTCGCCCAGGGCGACTGGGTCGCCCAGGCCGATATCTACGGCG
AGCGCTTGCAGTCCCCGGCCTCGCCGGCGCCGACGCGCGACGATCCCATCCGCGAGCTGATCGCCCAGGCGCAGAGCGAA
CAGGGCGACGCCCGCCCGACGCCGACCGCGGAACGTTCGACCGGCGGTGCCTGGGCGGTGCAGGTGGGCGCCTTCAGCGA
CCGCATGCGAGCGCGTCACCAAGCCGACCAGGCTGCCGACTTCCTCGATCTGGGTGGCCGCGTCGCGGTGTCTTCGACCC
AGGGAGCGCGCAGCATCTTCCGGGCACAGCTGGTGGATCTCGAGGAAAGCCAGGCGCGCCGTTCCTGTCAGCAGCTGGCA
CGCGAGGGAATCGACTGCGTGGTGGTGGCGTCGGGTCGCTGA

Upstream 100 bases:

>100_bases
TAATCCCTATGTCATTGCACTTTGTGGCGTCGCCATAACAAATGTCGCGGCAAATCTGGCGGATGGGCCCGTGGCGGGCC
CTCTTTGAAGGACATACAAC

Downstream 100 bases:

>100_bases
TGGGCGGCCGCCGAGGACGCTACGTCATGTCGACATCGGCACGAATGCAGATGGCGTCGTGCAGCCAGTGGTCCTCGTCG
TATTCGAGCGCGCGGCCGTC

Product: Serine-type D-Ala-D-Ala carboxypeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 5; PBP-5 [H]

Number of amino acids: Translated: 413; Mature: 412

Protein sequence:

>413_residues
MTSLHSQRARLLPALILLVVFAIALCTLSVAEAAPNPRYASIVIDTENGAVLHADNADAPRYPASLTKMMTLYLLFEAIE
DDTLSLESRLPVSQYAAAKPPSKMGVTPGETVKVEDAINILIVKSANDVAAVVAEGLAGSESVFAGQMTDKARALGMMDT
TFRNASGLPDAGQVTTARDMAILALRLMQDFPGYYPYFGKTQFTWQGKRYQGHNRLLDSYAGADGLKTGYIRASGFNVAT
SAVHDGRRILSVVMGGFTAQSRDAHMRELLDSGFARVASLAQGDWVAQADIYGERLQSPASPAPTRDDPIRELIAQAQSE
QGDARPTPTAERSTGGAWAVQVGAFSDRMRARHQADQAADFLDLGGRVAVSSTQGARSIFRAQLVDLEESQARRSCQQLA
REGIDCVVVASGR

Sequences:

>Translated_413_residues
MTSLHSQRARLLPALILLVVFAIALCTLSVAEAAPNPRYASIVIDTENGAVLHADNADAPRYPASLTKMMTLYLLFEAIE
DDTLSLESRLPVSQYAAAKPPSKMGVTPGETVKVEDAINILIVKSANDVAAVVAEGLAGSESVFAGQMTDKARALGMMDT
TFRNASGLPDAGQVTTARDMAILALRLMQDFPGYYPYFGKTQFTWQGKRYQGHNRLLDSYAGADGLKTGYIRASGFNVAT
SAVHDGRRILSVVMGGFTAQSRDAHMRELLDSGFARVASLAQGDWVAQADIYGERLQSPASPAPTRDDPIRELIAQAQSE
QGDARPTPTAERSTGGAWAVQVGAFSDRMRARHQADQAADFLDLGGRVAVSSTQGARSIFRAQLVDLEESQARRSCQQLA
REGIDCVVVASGR
>Mature_412_residues
TSLHSQRARLLPALILLVVFAIALCTLSVAEAAPNPRYASIVIDTENGAVLHADNADAPRYPASLTKMMTLYLLFEAIED
DTLSLESRLPVSQYAAAKPPSKMGVTPGETVKVEDAINILIVKSANDVAAVVAEGLAGSESVFAGQMTDKARALGMMDTT
FRNASGLPDAGQVTTARDMAILALRLMQDFPGYYPYFGKTQFTWQGKRYQGHNRLLDSYAGADGLKTGYIRASGFNVATS
AVHDGRRILSVVMGGFTAQSRDAHMRELLDSGFARVASLAQGDWVAQADIYGERLQSPASPAPTRDDPIRELIAQAQSEQ
GDARPTPTAERSTGGAWAVQVGAFSDRMRARHQADQAADFLDLGGRVAVSSTQGARSIFRAQLVDLEESQARRSCQQLAR
EGIDCVVVASGR

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein. Note=N-terminal lies in the periplasmic space (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1786851, Length=240, Percent_Identity=37.0833333333333, Blast_Score=147, Evalue=2e-36,
Organism=Escherichia coli, GI1787062, Length=266, Percent_Identity=36.4661654135338, Blast_Score=146, Evalue=3e-36,
Organism=Escherichia coli, GI87082022, Length=300, Percent_Identity=33, Blast_Score=139, Evalue=2e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 44299; Mature: 44167

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSLHSQRARLLPALILLVVFAIALCTLSVAEAAPNPRYASIVIDTENGAVLHADNADAP
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCEEEECCCCCCC
RYPASLTKMMTLYLLFEAIEDDTLSLESRLPVSQYAAAKPPSKMGVTPGETVKVEDAINI
CCCHHHHHHHHHHHHHHHHCCCCCCHHHCCCHHHHHCCCCCHHCCCCCCCCEEEECCEEE
LIVKSANDVAAVVAEGLAGSESVFAGQMTDKARALGMMDTTFRNASGLPDAGQVTTARDM
EEEECCCHHHHHHHHCCCCCCCEEECCHHHHHHHHCCHHHHHCCCCCCCCCCCCCHHHHH
AILALRLMQDFPGYYPYFGKTQFTWQGKRYQGHNRLLDSYAGADGLKTGYIRASGFNVAT
HHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCHHHHHHHHCCCCCCCCCEEEECCCCHHH
SAVHDGRRILSVVMGGFTAQSRDAHMRELLDSGFARVASLAQGDWVAQADIYGERLQSPA
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHCCCC
SPAPTRDDPIRELIAQAQSEQGDARPTPTAERSTGGAWAVQVGAFSDRMRARHQADQAAD
CCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHH
FLDLGGRVAVSSTQGARSIFRAQLVDLEESQARRSCQQLAREGIDCVVVASGR
HHHCCCEEEEECCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEEECCC
>Mature Secondary Structure 
TSLHSQRARLLPALILLVVFAIALCTLSVAEAAPNPRYASIVIDTENGAVLHADNADAP
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCEEEECCCCCCC
RYPASLTKMMTLYLLFEAIEDDTLSLESRLPVSQYAAAKPPSKMGVTPGETVKVEDAINI
CCCHHHHHHHHHHHHHHHHCCCCCCHHHCCCHHHHHCCCCCHHCCCCCCCCEEEECCEEE
LIVKSANDVAAVVAEGLAGSESVFAGQMTDKARALGMMDTTFRNASGLPDAGQVTTARDM
EEEECCCHHHHHHHHCCCCCCCEEECCHHHHHHHHCCHHHHHCCCCCCCCCCCCCHHHHH
AILALRLMQDFPGYYPYFGKTQFTWQGKRYQGHNRLLDSYAGADGLKTGYIRASGFNVAT
HHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCHHHHHHHHCCCCCCCCCEEEECCCCHHH
SAVHDGRRILSVVMGGFTAQSRDAHMRELLDSGFARVASLAQGDWVAQADIYGERLQSPA
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHCCCC
SPAPTRDDPIRELIAQAQSEQGDARPTPTAERSTGGAWAVQVGAFSDRMRARHQADQAAD
CCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHH
FLDLGGRVAVSSTQGARSIFRAQLVDLEESQARRSCQQLAREGIDCVVVASGR
HHHCCCEEEEECCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]