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The map label for this gene is ytxM [H]

Identifier: 92112274

GI number: 92112274

Start: 160347

End: 161201

Strand: Direct

Name: ytxM [H]

Synonym: Csal_0139

Alternate gene names: 92112274

Gene position: 160347-161201 (Clockwise)

Preceding gene: 92112273

Following gene: 92112275

Centisome position: 4.34

GC content: 68.3

Gene sequence:

>855_bases
ATGAACGTGCCGGACGACGCCCTCGAGGCGGCGGAAGTCCAGTGTCGCGAGGTGTCGCTTGCGTCCGGACGTCAGGCGTT
TTGCGACATGGGCAGTGGCATCCCCGTCGTGCTGTTGCATGGCATCAGTTCCGGAGCCCGTTCCTGGGCACCGCTGATGC
ATCAGGCGACGGGAGTCCGCTGGCTGGCCTGGGACGCGCCGGGCTACGGCGAGAGCTCGGCGCTGGCCGAGCCCCATCCC
ACGGCACGGGACTATGCCTTGCGGCTGGCGGCATGGCTGGAAGCGCTGGCACTCGAGCGCGTGGTGCTGATCGGCCACTC
ACTGGGTGCGTTGATCGCGAGTGCCTATGCCCGTGACTTCCCTGACCGGGTCTCGGGGCTGCTGCTGGCCGACCCTGCCC
AGGGCTATCGCCATGCCGATCCCGACAAGCGCGATGCCGTTTACCGGAGTCGTTGGACGCAACTCGCCGCGCAAGGGCAC
GCGGCCTACGCAGCGGCGCGAGCGCCCAGGCTGTTGCGCGAGAACGCTCGCGTGGAAGATATCGCGCGTGTCCAGGCCGG
CATGCACCGCCTCGAGGTGTCGGGATTCGCCCAGGCCAGCTGGATGCTGGCCAACGACTCGCTGGAGGACCATGCGAGCG
GCACGTCCGTGCCCACTCGGGTGCTATGCGGCGACGAGGACCGCATCACGCCGCCGAGCGGTGCGCGTGCCCTGGCGGAG
CGTCTCGGGGTCGCGTATCGCGACATCCCGTGTGCCGGGCACATCAGTTATATCGACGCTCCCGCTGCTTTCGCCGCCGC
CGTTGCGGATTTCATGGCGACGCTGTCGCCGAGCCAGGAGAAAAGCGACTTATGA

Upstream 100 bases:

>100_bases
GCGAGGAAGAGGCACTCATGTGCGTCATGCTGGGCACACCCAAACCGCAGATTCCGACGTATCCGGACGACCATCCGCTA
TCCAAGATCAAGCGGCAATC

Downstream 100 bases:

>100_bases
GTTTTCAGATCGAACGACGTGTGGCCGTGGTCACCGGTGGCTCCTCGGGAATCGGACTCGAAACCGTGCGCCTGCTGCTC
GAATCGGGCGCCAGCGTGGC

Product: alpha/beta hydrolase

Products: 2-oxopent-4-enoate; succinate [C]

Alternate protein names: NA

Number of amino acids: Translated: 284; Mature: 284

Protein sequence:

>284_residues
MNVPDDALEAAEVQCREVSLASGRQAFCDMGSGIPVVLLHGISSGARSWAPLMHQATGVRWLAWDAPGYGESSALAEPHP
TARDYALRLAAWLEALALERVVLIGHSLGALIASAYARDFPDRVSGLLLADPAQGYRHADPDKRDAVYRSRWTQLAAQGH
AAYAAARAPRLLRENARVEDIARVQAGMHRLEVSGFAQASWMLANDSLEDHASGTSVPTRVLCGDEDRITPPSGARALAE
RLGVAYRDIPCAGHISYIDAPAAFAAAVADFMATLSPSQEKSDL

Sequences:

>Translated_284_residues
MNVPDDALEAAEVQCREVSLASGRQAFCDMGSGIPVVLLHGISSGARSWAPLMHQATGVRWLAWDAPGYGESSALAEPHP
TARDYALRLAAWLEALALERVVLIGHSLGALIASAYARDFPDRVSGLLLADPAQGYRHADPDKRDAVYRSRWTQLAAQGH
AAYAAARAPRLLRENARVEDIARVQAGMHRLEVSGFAQASWMLANDSLEDHASGTSVPTRVLCGDEDRITPPSGARALAE
RLGVAYRDIPCAGHISYIDAPAAFAAAVADFMATLSPSQEKSDL
>Mature_284_residues
MNVPDDALEAAEVQCREVSLASGRQAFCDMGSGIPVVLLHGISSGARSWAPLMHQATGVRWLAWDAPGYGESSALAEPHP
TARDYALRLAAWLEALALERVVLIGHSLGALIASAYARDFPDRVSGLLLADPAQGYRHADPDKRDAVYRSRWTQLAAQGH
AAYAAARAPRLLRENARVEDIARVQAGMHRLEVSGFAQASWMLANDSLEDHASGTSVPTRVLCGDEDRITPPSGARALAE
RLGVAYRDIPCAGHISYIDAPAAFAAAVADFMATLSPSQEKSDL

Specific function: 3-hydroxyphenylpropionate degradation. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lipase/esterase LIP3/BchO family [H]

Homologues:

Organism=Homo sapiens, GI50658087, Length=141, Percent_Identity=29.7872340425532, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17558492, Length=112, Percent_Identity=33.0357142857143, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI32566936, Length=112, Percent_Identity=33.0357142857143, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI25146278, Length=118, Percent_Identity=30.5084745762712, Blast_Score=70, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR000639
- InterPro:   IPR022485 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: 3.7.1.- [C]

Molecular weight: Translated: 30362; Mature: 30362

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: PS00120 LIPASE_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVPDDALEAAEVQCREVSLASGRQAFCDMGSGIPVVLLHGISSGARSWAPLMHQATGVR
CCCCHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHCCCE
WLAWDAPGYGESSALAEPHPTARDYALRLAAWLEALALERVVLIGHSLGALIASAYARDF
EEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PDRVSGLLLADPAQGYRHADPDKRDAVYRSRWTQLAAQGHAAYAAARAPRLLRENARVED
HHHHCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHH
IARVQAGMHRLEVSGFAQASWMLANDSLEDHASGTSVPTRVLCGDEDRITPPSGARALAE
HHHHHHHHHHHHHCCHHHHEEEEECCCCHHHCCCCCCCEEEEECCCCCCCCCCHHHHHHH
RLGVAYRDIPCAGHISYIDAPAAFAAAVADFMATLSPSQEKSDL
HHCCHHHCCCCCCCCHHHCCHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MNVPDDALEAAEVQCREVSLASGRQAFCDMGSGIPVVLLHGISSGARSWAPLMHQATGVR
CCCCHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHCCCE
WLAWDAPGYGESSALAEPHPTARDYALRLAAWLEALALERVVLIGHSLGALIASAYARDF
EEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PDRVSGLLLADPAQGYRHADPDKRDAVYRSRWTQLAAQGHAAYAAARAPRLLRENARVED
HHHHCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHH
IARVQAGMHRLEVSGFAQASWMLANDSLEDHASGTSVPTRVLCGDEDRITPPSGARALAE
HHHHHHHHHHHHHCCHHHHEEEEECCCCHHHCCCCCCCEEEEECCCCCCCCCCHHHHHHH
RLGVAYRDIPCAGHISYIDAPAAFAAAVADFMATLSPSQEKSDL
HHCCHHHCCCCCCCCHHHCCHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-hydroxy-6-ketononadienedicarboxylate; H2O [C]

Specific reaction: 2-hydroxy-6-ketononadienedicarboxylate + H2O = 2-oxopent-4-enoate + succinate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8566759; 9387221; 9384377 [H]