Click here to switch to the map view.

The map label for this gene is pcm [H]

Identifier: 91788920

GI number: 91788920

Start: 3225092

End: 3225850

Strand: Reverse

Name: pcm [H]

Synonym: Bpro_3060

Alternate gene names: 91788920

Gene position: 3225850-3225092 (Counterclockwise)

Preceding gene: 91788921

Following gene: 91788919

Centisome position: 62.03

GC content: 63.37

Gene sequence:

>759_bases
ATGCCGTCTGCTACTAAATTGGTAGCTACTGCAGCACGTCCTACGGGAAATGCAGCAAAAAATGGTTTCAAGAAATCAGT
GCCAGCGGCAGCGCCTGACGGTGTCGGACTGGATTCCCACGCGGTGCGTGCGCGCATGGTGCACAAGCTCGCGGCACAGG
GCGTATCAGACGCCCGGGTGCTGGCTGCCATGGGCCTGGTGGAGCGCCACCGCTTTGTCGATACCGCCCTGGTCAACCAG
GCGTATGAGGACACCAGCCTGCCGATTGGCCTGGGCCAGACCATTTCCAAGCCCAACGTCGTGGCGCGCATGCTGGAACT
GCTTTGCCAGGGGGCGGCGGGCAAGCTGGACCGGTTGGGAAGGGTGCTGGAGATCGGTACCGGCTGTGGATACCAGGCCG
CCCTGCTCAGCCACCTGGCAAACGAGGTCTACAGCATCGAGCGGCTGCGCGGCCTGCATGACAAGGCCCGGGAAAACCTG
CGGGCTTTGCGCCTGCCCAATGTCCACCTGTTGTTCGGTGATGGCATGATCGGGTACGCCAAGGGAGCGCCGTACGCGGC
CATTATTGCGGCGGCGGGCGGTGAAGCCATTCCGCCCGCCTGGGTGGAGCAACTGGCCGTTGGCGGGCGTCTTGTCGCGC
CCTTGCAAACTGCCGCCGGCGTGCAGGCGCTGGTGGTGGTGGACAAAACGCCCCTGGGCGTCAAGCAGACAGTGCTGGAA
GCCGTCCATTTTGTACCTTTAAAATCGGGTACGAGCTGA

Upstream 100 bases:

>100_bases
GATGAAATCCAGCTTCAAGCCTCCTTTCAATCCCACCGCCAAAGCACCTGCCGTGGCGAGCCGTCCCGCATTTCCGGTGC
GATTGAAACCCGGGGAGGGC

Downstream 100 bases:

>100_bases
AGATGGCTTGAACAGCCCATGAAGATCTCATGAAATATCCCATGAAGATCGAGTCCATATTGAAGTCATATTGAAGTCAA
GAAGACGAAAGAACCAATGA

Product: protein-L-isoaspartate O-methyltransferase

Products: NA

Alternate protein names: L-isoaspartyl protein carboxyl methyltransferase; Protein L-isoaspartyl methyltransferase; Protein-beta-aspartate methyltransferase; PIMT [H]

Number of amino acids: Translated: 252; Mature: 251

Protein sequence:

>252_residues
MPSATKLVATAARPTGNAAKNGFKKSVPAAAPDGVGLDSHAVRARMVHKLAAQGVSDARVLAAMGLVERHRFVDTALVNQ
AYEDTSLPIGLGQTISKPNVVARMLELLCQGAAGKLDRLGRVLEIGTGCGYQAALLSHLANEVYSIERLRGLHDKARENL
RALRLPNVHLLFGDGMIGYAKGAPYAAIIAAAGGEAIPPAWVEQLAVGGRLVAPLQTAAGVQALVVVDKTPLGVKQTVLE
AVHFVPLKSGTS

Sequences:

>Translated_252_residues
MPSATKLVATAARPTGNAAKNGFKKSVPAAAPDGVGLDSHAVRARMVHKLAAQGVSDARVLAAMGLVERHRFVDTALVNQ
AYEDTSLPIGLGQTISKPNVVARMLELLCQGAAGKLDRLGRVLEIGTGCGYQAALLSHLANEVYSIERLRGLHDKARENL
RALRLPNVHLLFGDGMIGYAKGAPYAAIIAAAGGEAIPPAWVEQLAVGGRLVAPLQTAAGVQALVVVDKTPLGVKQTVLE
AVHFVPLKSGTS
>Mature_251_residues
PSATKLVATAARPTGNAAKNGFKKSVPAAAPDGVGLDSHAVRARMVHKLAAQGVSDARVLAAMGLVERHRFVDTALVNQA
YEDTSLPIGLGQTISKPNVVARMLELLCQGAAGKLDRLGRVLEIGTGCGYQAALLSHLANEVYSIERLRGLHDKARENLR
ALRLPNVHLLFGDGMIGYAKGAPYAAIIAAAGGEAIPPAWVEQLAVGGRLVAPLQTAAGVQALVVVDKTPLGVKQTVLEA
VHFVPLKSGTS

Specific function: Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins [H]

COG id: COG2518

COG function: function code O; Protein-L-isoaspartate carboxylmethyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. L- isoaspartyl/D-aspartyl protein methyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI226530908, Length=217, Percent_Identity=31.7972350230415, Blast_Score=88, Evalue=8e-18,
Organism=Escherichia coli, GI1789100, Length=205, Percent_Identity=44.8780487804878, Blast_Score=150, Evalue=9e-38,
Organism=Caenorhabditis elegans, GI71983477, Length=199, Percent_Identity=32.1608040201005, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI193207222, Length=199, Percent_Identity=30.1507537688442, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI17981723, Length=218, Percent_Identity=32.1100917431193, Blast_Score=74, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000682 [H]

Pfam domain/function: PF01135 PCMT [H]

EC number: =2.1.1.77 [H]

Molecular weight: Translated: 26225; Mature: 26094

Theoretical pI: Translated: 10.17; Mature: 10.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSATKLVATAARPTGNAAKNGFKKSVPAAAPDGVGLDSHAVRARMVHKLAAQGVSDARV
CCCCHHHHHCCCCCCCHHHHCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHH
LAAMGLVERHRFVDTALVNQAYEDTSLPIGLGQTISKPNVVARMLELLCQGAAGKLDRLG
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHCC
RVLEIGTGCGYQAALLSHLANEVYSIERLRGLHDKARENLRALRLPNVHLLFGDGMIGYA
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEECC
KGAPYAAIIAAAGGEAIPPAWVEQLAVGGRLVAPLQTAAGVQALVVVDKTPLGVKQTVLE
CCCCCEEEEEECCCCCCCHHHHHHHHHCCEEEHHHHHHCCCEEEEEECCCCCCHHHHHHH
AVHFVPLKSGTS
HHHHHCCCCCCC
>Mature Secondary Structure 
PSATKLVATAARPTGNAAKNGFKKSVPAAAPDGVGLDSHAVRARMVHKLAAQGVSDARV
CCCHHHHHCCCCCCCHHHHCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHH
LAAMGLVERHRFVDTALVNQAYEDTSLPIGLGQTISKPNVVARMLELLCQGAAGKLDRLG
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHCC
RVLEIGTGCGYQAALLSHLANEVYSIERLRGLHDKARENLRALRLPNVHLLFGDGMIGYA
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEECC
KGAPYAAIIAAAGGEAIPPAWVEQLAVGGRLVAPLQTAAGVQALVVVDKTPLGVKQTVLE
CCCCCEEEEEECCCCCCCHHHHHHHHHCCEEEHHHHHHCCCEEEEEECCCCCCHHHHHHH
AVHFVPLKSGTS
HHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA