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The map label for this gene is rumA

Identifier: 91788917

GI number: 91788917

Start: 3221858

End: 3223318

Strand: Reverse

Name: rumA

Synonym: Bpro_3057

Alternate gene names: 91788917

Gene position: 3223318-3221858 (Counterclockwise)

Preceding gene: 91788919

Following gene: 91788915

Centisome position: 61.98

GC content: 61.26

Gene sequence:

>1461_bases
ATGACCGATGAAAAACTGAATGACCACACTGTGAAAGCGCCTGAATACCTGCCAGACATCCTCACGGTAGAGTCCCTGGA
CATGGAAGCGCAGGGCATTGCCCACCGGGCAGACGGTAAGGTTGTGTTCATAGAGGGTGCGCTGCCTTTTGAACGGGTGA
CCGCCAATGTGTATCGCAAGAAGAGCAGCTTTGAAAAAGCGGTGGTGATGGCTATTCATCGCGAGTCTTCGCAGCGCGTG
CGCCCGGCTTGCCCGCATTTTGGCCTGCATACCGGCGCGTGCGGCGGCTGCAAGATGCAGCACCTTCATGTGGGTGCGCA
GGTGGCTGTCAAGCAGCGGGTGCTGGAAGACAACCTCTGGCACATCGGCAAGGTCAAGGCCGACAACCTGCTCAGGCCCA
TTGAGGGGCCCGCCTGGGGGTATCGCTACCGGGCGCGCCTGTCGGTGCGCTATGTGCGCAAGAAGAATGCGGTGCTGGTG
GGCTTTCATGAACGCAAGAGCAGTTATGTGGCCGACATGACTGAGTGCCATGTCGTGCCCCGGCATGTCAGCGACATGCT
GGTGCCGCTGCGTGAACTGATTGCCGGCATGGATGCCAGGGAAACCCTGCCGCAAATCGAGTTGGCCTGCGGCGATACCG
TCACGGCCATGGTGCTACGCCACATGGAGCCGCTCAGCAGCGGCGATCTGGCACGCTTGCGCGCTTTCGCGGTATCGCAT
CCAGGCCTGCAGTGGTGGCTGCAGCCAGGTGGCCTGGACAGCGTGAAGCTGCTCGACGAGGGCGTACCCGAGTTGAGCTA
CGACCTGCCCGAGTTTGGCATCACGATGCCCTTCAAGCCGACCGATTTCACGCAGGTGAACCCGCACATCAACCAGGTGC
TGGTGTCGCGGGCGTTGCGCCTGCTGGCCGTGCAGCCGCATGAGCGTGTGATTGACTGGTTTTGCGGCCTGGGGAATTTC
ACACTGCCGCTGGCGACTTGCGCACGCGAAGTGCTGGGTATTGAAGGCAGCGAGGCCCTGGTGGCACGCTCCTGCCAGAA
CTTCAAGAAGAATCAGCCGGCTTCGCAATCAAGAAGCGCGCTGTCTGCTACTGAATTCGTGGCAAGAAACCTCTTTGAAA
TGACACCCGCGATGCTGGTGAAAGATGGTATGGCCGACAAGTGGCTGGTGGATCCGCCGCGCGAGGGGGCATTCGAGCTG
TTCAAGTCGCTGGCGGCGCTGCATCAGCAAATGGTGACGGGCGTGCCCTGCGACGACGGGGAACAACAGCAAAGCCTGGT
GCTGGGCGACTGGACGCCGCCCCAGCGCATTGTTTACGTGAGCTGCAACCCGGCGACCCTGGCGCGCGATGCTGGCGTAT
TAGTGGAGAGCGGCGCTTACCGCTGCACGCTGGCTGGTGTGGTGAACATGTTTCCCCATACGGCGCATGTGGAGAGCATC
GCGGTGTTTGAGCGGGGTTGA

Upstream 100 bases:

>100_bases
GAACGGTATTGCGGTGCGGCGCGATGTGCGGCGCCCATTCGAACCACGATGCTGAACCCTGCGGACATCGCTCCGGAGTC
GGACCTGAGACAATTCAGGC

Downstream 100 bases:

>100_bases
GCGGGCATGAAAAAAGGGCCCGCAGGCCCTTTTTTTGGGGGAGGCAGGACGCCGCGCGGCGTCAGTCGCGCTCGCCGCCA
AAGATGCCCAGCAGCGCCAG

Product: 23S rRNA 5-methyluridine methyltransferase

Products: NA

Alternate protein names: 23S rRNA(M-5-U1939)-methyltransferase

Number of amino acids: Translated: 486; Mature: 485

Protein sequence:

>486_residues
MTDEKLNDHTVKAPEYLPDILTVESLDMEAQGIAHRADGKVVFIEGALPFERVTANVYRKKSSFEKAVVMAIHRESSQRV
RPACPHFGLHTGACGGCKMQHLHVGAQVAVKQRVLEDNLWHIGKVKADNLLRPIEGPAWGYRYRARLSVRYVRKKNAVLV
GFHERKSSYVADMTECHVVPRHVSDMLVPLRELIAGMDARETLPQIELACGDTVTAMVLRHMEPLSSGDLARLRAFAVSH
PGLQWWLQPGGLDSVKLLDEGVPELSYDLPEFGITMPFKPTDFTQVNPHINQVLVSRALRLLAVQPHERVIDWFCGLGNF
TLPLATCAREVLGIEGSEALVARSCQNFKKNQPASQSRSALSATEFVARNLFEMTPAMLVKDGMADKWLVDPPREGAFEL
FKSLAALHQQMVTGVPCDDGEQQQSLVLGDWTPPQRIVYVSCNPATLARDAGVLVESGAYRCTLAGVVNMFPHTAHVESI
AVFERG

Sequences:

>Translated_486_residues
MTDEKLNDHTVKAPEYLPDILTVESLDMEAQGIAHRADGKVVFIEGALPFERVTANVYRKKSSFEKAVVMAIHRESSQRV
RPACPHFGLHTGACGGCKMQHLHVGAQVAVKQRVLEDNLWHIGKVKADNLLRPIEGPAWGYRYRARLSVRYVRKKNAVLV
GFHERKSSYVADMTECHVVPRHVSDMLVPLRELIAGMDARETLPQIELACGDTVTAMVLRHMEPLSSGDLARLRAFAVSH
PGLQWWLQPGGLDSVKLLDEGVPELSYDLPEFGITMPFKPTDFTQVNPHINQVLVSRALRLLAVQPHERVIDWFCGLGNF
TLPLATCAREVLGIEGSEALVARSCQNFKKNQPASQSRSALSATEFVARNLFEMTPAMLVKDGMADKWLVDPPREGAFEL
FKSLAALHQQMVTGVPCDDGEQQQSLVLGDWTPPQRIVYVSCNPATLARDAGVLVESGAYRCTLAGVVNMFPHTAHVESI
AVFERG
>Mature_485_residues
TDEKLNDHTVKAPEYLPDILTVESLDMEAQGIAHRADGKVVFIEGALPFERVTANVYRKKSSFEKAVVMAIHRESSQRVR
PACPHFGLHTGACGGCKMQHLHVGAQVAVKQRVLEDNLWHIGKVKADNLLRPIEGPAWGYRYRARLSVRYVRKKNAVLVG
FHERKSSYVADMTECHVVPRHVSDMLVPLRELIAGMDARETLPQIELACGDTVTAMVLRHMEPLSSGDLARLRAFAVSHP
GLQWWLQPGGLDSVKLLDEGVPELSYDLPEFGITMPFKPTDFTQVNPHINQVLVSRALRLLAVQPHERVIDWFCGLGNFT
LPLATCAREVLGIEGSEALVARSCQNFKKNQPASQSRSALSATEFVARNLFEMTPAMLVKDGMADKWLVDPPREGAFELF
KSLAALHQQMVTGVPCDDGEQQQSLVLGDWTPPQRIVYVSCNPATLARDAGVLVESGAYRCTLAGVVNMFPHTAHVESIA
VFERG

Specific function: Catalyzes the formation of 5-methyl-uridine at position 1939 (M-5-U1939) in 23S rRNA

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Escherichia coli, GI1789148, Length=470, Percent_Identity=37.8723404255319, Blast_Score=267, Evalue=9e-73,
Organism=Drosophila melanogaster, GI24666579, Length=229, Percent_Identity=27.5109170305677, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUMA_POLSJ (Q128S1)

Other databases:

- EMBL:   CP000316
- RefSeq:   YP_549869.1
- ProteinModelPortal:   Q128S1
- STRING:   Q128S1
- GeneID:   4014287
- GenomeReviews:   CP000316_GR
- KEGG:   pol:Bpro_3057
- NMPDR:   fig|296591.1.peg.2070
- eggNOG:   COG2265
- HOGENOM:   HBG690163
- OMA:   HRADGKV
- PhylomeDB:   Q128S1
- ProtClustDB:   PRK13168
- BioCyc:   PSP296591:BPRO_3057-MONOMER
- HAMAP:   MF_01010
- InterPro:   IPR016027
- InterPro:   IPR001566
- InterPro:   IPR010280
- TIGRFAMs:   TIGR00479

Pfam domain/function: PF05958 tRNA_U5-meth_tr; SSF50249 Nucleic_acid_OB

EC number: =2.1.1.190

Molecular weight: Translated: 53839; Mature: 53707

Theoretical pI: Translated: 7.13; Mature: 7.13

Prosite motif: PS50926 TRAM; PS01230 TRMA_1; PS01231 TRMA_2

Important sites: ACT_SITE 442-442 BINDING 285-285 BINDING 314-314 BINDING 319-319 BINDING 335-335 BINDING 370-370 BINDING 391-391

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDEKLNDHTVKAPEYLPDILTVESLDMEAQGIAHRADGKVVFIEGALPFERVTANVYRK
CCCCCCCCCCCCCHHHCCCHHHHHCCCCHHHCCEEECCCCEEEEECCCCHHHHHHHHHHH
KSSFEKAVVMAIHRESSQRVRPACPHFGLHTGACGGCKMQHLHVGAQVAVKQRVLEDNLW
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHCCCC
HIGKVKADNLLRPIEGPAWGYRYRARLSVRYVRKKNAVLVGFHERKSSYVADMTECHVVP
CCCCCCCCCCCCCCCCCCCCEEEEEEEEEEEEECCCCEEEEEHHHCCHHHHHHHHHHHHH
RHVSDMLVPLRELIAGMDARETLPQIELACGDTVTAMVLRHMEPLSSGDLARLRAFAVSH
HHHHHHHHHHHHHHCCCCHHHHCCCEEECCCCHHHHHHHHHHCCCCCCCHHHHHHHHHCC
PGLQWWLQPGGLDSVKLLDEGVPELSYDLPEFGITMPFKPTDFTQVNPHINQVLVSRALR
CCCEEEECCCCCCHHHHHHCCCCHHCCCCCCCCEECCCCCCCCCCCCHHHHHHHHHHHHH
LLAVQPHERVIDWFCGLGNFTLPLATCAREVLGIEGSEALVARSCQNFKKNQPASQSRSA
HHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHH
LSATEFVARNLFEMTPAMLVKDGMADKWLVDPPREGAFELFKSLAALHQQMVTGVPCDDG
HHHHHHHHHHHHHHCHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCC
EQQQSLVLGDWTPPQRIVYVSCNPATLARDAGVLVESGAYRCTLAGVVNMFPHTAHVESI
CCCCCEEEECCCCCCEEEEEECCCHHHHCCCCEEEECCCCEEEHHHHHHHCCCHHHHHEE
AVFERG
EEEECC
>Mature Secondary Structure 
TDEKLNDHTVKAPEYLPDILTVESLDMEAQGIAHRADGKVVFIEGALPFERVTANVYRK
CCCCCCCCCCCCHHHCCCHHHHHCCCCHHHCCEEECCCCEEEEECCCCHHHHHHHHHHH
KSSFEKAVVMAIHRESSQRVRPACPHFGLHTGACGGCKMQHLHVGAQVAVKQRVLEDNLW
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHCCCC
HIGKVKADNLLRPIEGPAWGYRYRARLSVRYVRKKNAVLVGFHERKSSYVADMTECHVVP
CCCCCCCCCCCCCCCCCCCCEEEEEEEEEEEEECCCCEEEEEHHHCCHHHHHHHHHHHHH
RHVSDMLVPLRELIAGMDARETLPQIELACGDTVTAMVLRHMEPLSSGDLARLRAFAVSH
HHHHHHHHHHHHHHCCCCHHHHCCCEEECCCCHHHHHHHHHHCCCCCCCHHHHHHHHHCC
PGLQWWLQPGGLDSVKLLDEGVPELSYDLPEFGITMPFKPTDFTQVNPHINQVLVSRALR
CCCEEEECCCCCCHHHHHHCCCCHHCCCCCCCCEECCCCCCCCCCCCHHHHHHHHHHHHH
LLAVQPHERVIDWFCGLGNFTLPLATCAREVLGIEGSEALVARSCQNFKKNQPASQSRSA
HHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHH
LSATEFVARNLFEMTPAMLVKDGMADKWLVDPPREGAFELFKSLAALHQQMVTGVPCDDG
HHHHHHHHHHHHHHCHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCC
EQQQSLVLGDWTPPQRIVYVSCNPATLARDAGVLVESGAYRCTLAGVVNMFPHTAHVESI
CCCCCEEEECCCCCCEEEEEECCCHHHHCCCCEEEECCCCEEEHHHHHHHCCCHHHHHEE
AVFERG
EEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA