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The map label for this gene is rlmB [H]

Identifier: 91788889

GI number: 91788889

Start: 3194064

End: 3194858

Strand: Reverse

Name: rlmB [H]

Synonym: Bpro_3029

Alternate gene names: 91788889

Gene position: 3194858-3194064 (Counterclockwise)

Preceding gene: 91788890

Following gene: 91788888

Centisome position: 61.44

GC content: 65.28

Gene sequence:

>795_bases
ATGTCGGCATGTCGGCATGTCGGCATGTGCAGTGATAATCCTTGTATGTCCTCTTCCCCCAAAGTTCTTTTTGGCTTTCA
TGCCGTGGGCGTGCGCCTTAAAACCGCGCCCCAATCGGTTCTGGAGGTTTTTTTCGATGCCTCGCGCCGCGATGCCCGCA
TGCGCCAGTTCACCGACCGCGCCCGCGAAGCCGGCGTTCGCTTGATTGAGTCCGACGGCCTGCGCCTGGCCAAAATGTGT
GGCAGCCACGGCCACCAGGGTGTCGTCGCCCGCGTCGATGCGGTGGCCCAGGTCAAGTCCCTCGATGAGCTGCTGGAGCA
ACTGGAAGAGGCCGGCACCGCGCAGCCCCTGCTGCTGGTGCTCGACGGCGTGACCGATCCGCACAACCTGGGCGCCTGCC
TGCGGGTGGCCGACGGCGCGGGCGCCCATGCCGTGATCGCCCCCAAGGACCATGCCGCCGGCATCAACGCCACAGTGGCC
AAGGTTGCCAGCGGCGCGGCCGAGACCATGCCTTACTTCATGGTCACCAACCTGGCCCGCACGCTGGGCGAACTCAAGGA
GCGCAACATCTGGTGCATAGGCACCAGCGACGACGCCCCCAAAACGATTTACGACGTGGACCTCAAGGGCCCGGTTGCGC
TGGTGCTCGGCGCTGAAGGAGAGGGCATGCGCCAGCTCACGCGCAAGACCTGCGACGAACTGGTCAGCATTCCGATGCGC
GGGGCTGTCGAAAGCCTCAACGTGTCGGTGGCCAGCGGCGTTTGCCTGTACGAAGCGCTGCGCCAGCGCAGCTGA

Upstream 100 bases:

>100_bases
TCAAGGCTTGATCGAAAAAACGATCCTGGGGCAAGTAATGCGTTTCCCCTTTGCCATTGCTTGCCTGGCAATTTGACGGC
GGGCAGGGTGGCCTGTCGGT

Downstream 100 bases:

>100_bases
CCGGGCTTCTGTCACCCGCATTTTCCTGGAGATGACTACGATGAATTTCCGGTACTTGATTGCTCCTGTTTTAATAGCTG
TCGGTGTGTTAACCATGCTG

Product: RNA methyltransferase TrmH

Products: NA

Alternate protein names: 23S rRNA Gm2251 2'-O-methyltransferase [H]

Number of amino acids: Translated: 264; Mature: 263

Protein sequence:

>264_residues
MSACRHVGMCSDNPCMSSSPKVLFGFHAVGVRLKTAPQSVLEVFFDASRRDARMRQFTDRAREAGVRLIESDGLRLAKMC
GSHGHQGVVARVDAVAQVKSLDELLEQLEEAGTAQPLLLVLDGVTDPHNLGACLRVADGAGAHAVIAPKDHAAGINATVA
KVASGAAETMPYFMVTNLARTLGELKERNIWCIGTSDDAPKTIYDVDLKGPVALVLGAEGEGMRQLTRKTCDELVSIPMR
GAVESLNVSVASGVCLYEALRQRS

Sequences:

>Translated_264_residues
MSACRHVGMCSDNPCMSSSPKVLFGFHAVGVRLKTAPQSVLEVFFDASRRDARMRQFTDRAREAGVRLIESDGLRLAKMC
GSHGHQGVVARVDAVAQVKSLDELLEQLEEAGTAQPLLLVLDGVTDPHNLGACLRVADGAGAHAVIAPKDHAAGINATVA
KVASGAAETMPYFMVTNLARTLGELKERNIWCIGTSDDAPKTIYDVDLKGPVALVLGAEGEGMRQLTRKTCDELVSIPMR
GAVESLNVSVASGVCLYEALRQRS
>Mature_263_residues
SACRHVGMCSDNPCMSSSPKVLFGFHAVGVRLKTAPQSVLEVFFDASRRDARMRQFTDRAREAGVRLIESDGLRLAKMCG
SHGHQGVVARVDAVAQVKSLDELLEQLEEAGTAQPLLLVLDGVTDPHNLGACLRVADGAGAHAVIAPKDHAAGINATVAK
VASGAAETMPYFMVTNLARTLGELKERNIWCIGTSDDAPKTIYDVDLKGPVALVLGAEGEGMRQLTRKTCDELVSIPMRG
AVESLNVSVASGVCLYEALRQRS

Specific function: Specifically methylates the ribose of guanosine 2251 in 23S rRNA [H]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. RlmB subfamily [H]

Homologues:

Organism=Homo sapiens, GI40068479, Length=276, Percent_Identity=29.7101449275362, Blast_Score=91, Evalue=1e-18,
Organism=Escherichia coli, GI1790623, Length=246, Percent_Identity=51.219512195122, Blast_Score=241, Evalue=3e-65,
Organism=Escherichia coli, GI1790083, Length=157, Percent_Identity=36.9426751592357, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1788935, Length=248, Percent_Identity=26.6129032258064, Blast_Score=77, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6324775, Length=165, Percent_Identity=33.9393939393939, Blast_Score=80, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004441
- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 28127; Mature: 27995

Theoretical pI: Translated: 6.85; Mature: 6.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSACRHVGMCSDNPCMSSSPKVLFGFHAVGVRLKTAPQSVLEVFFDASRRDARMRQFTDR
CCCCCCCCCCCCCCCCCCCCCEEEEEHHHCEEEECCHHHHHHHHHCCCCHHHHHHHHHHH
AREAGVRLIESDGLRLAKMCGSHGHQGVVARVDAVAQVKSLDELLEQLEEAGTAQPLLLV
HHHHCCEEEECCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEE
LDGVTDPHNLGACLRVADGAGAHAVIAPKDHAAGINATVAKVASGAAETMPYFMVTNLAR
EECCCCCCCCCCEEEECCCCCCEEEECCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHH
TLGELKERNIWCIGTSDDAPKTIYDVDLKGPVALVLGAEGEGMRQLTRKTCDELVSIPMR
HHHHHHHCCEEEEECCCCCCCEEEEECCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCC
GAVESLNVSVASGVCLYEALRQRS
CCHHHHCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SACRHVGMCSDNPCMSSSPKVLFGFHAVGVRLKTAPQSVLEVFFDASRRDARMRQFTDR
CCCCCCCCCCCCCCCCCCCCEEEEEHHHCEEEECCHHHHHHHHHCCCCHHHHHHHHHHH
AREAGVRLIESDGLRLAKMCGSHGHQGVVARVDAVAQVKSLDELLEQLEEAGTAQPLLLV
HHHHCCEEEECCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEE
LDGVTDPHNLGACLRVADGAGAHAVIAPKDHAAGINATVAKVASGAAETMPYFMVTNLAR
EECCCCCCCCCCEEEECCCCCCEEEECCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHH
TLGELKERNIWCIGTSDDAPKTIYDVDLKGPVALVLGAEGEGMRQLTRKTCDELVSIPMR
HHHHHHHCCEEEEECCCCCCCEEEEECCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCC
GAVESLNVSVASGVCLYEALRQRS
CCHHHHCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]