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The map label for this gene is ansB [H]

Identifier: 91788813

GI number: 91788813

Start: 3113666

End: 3114616

Strand: Reverse

Name: ansB [H]

Synonym: Bpro_2953

Alternate gene names: 91788813

Gene position: 3114616-3113666 (Counterclockwise)

Preceding gene: 91788823

Following gene: 91788806

Centisome position: 59.89

GC content: 64.35

Gene sequence:

>951_bases
ATGAATCGTCAAACCATTGTGATTTTGGGTACCGGCGGCACGATTGCCGGTCGCGCAGCCTCGGCCTCGGACAATATCGG
TTACACCGCGGCGGAAGTCGGTATTGAGCAGCTGGTGGCGGCGATTCCCGGGCTGGCCGATGCCGGCCCATTGATCGCCG
AGCAGGTTGCGCAGCTTGACAGCAAGGACATGAGCTTTGCCGTGTGGGCGAAACTGGCCGGACGCGTCAACCATTTTCTG
GCGCAGCCTGATGTCGCAGGGATCGTTGTTGTCCACGGCACCGACACGCTCGAAGAGACCGCGTATTTTTTACAGGCAGT
CTGCGGTCCCGCCAAGCCGGTGGTGCTGACCTGTGCCATGCGCCCGGCAACGGCCCTGGTACCGGACGGCCCGCAGAACG
TGCTCGATGCCGTGGCCGTGGCCCGGCACTCCGGGGCGAAAGGGGTGGTGGCCGTCTGCGCTGGCTCCGTCCACACTGCG
CGGGACGTGCAGAAGGTGCATCCCTACCAGGTGGACGCGTTCAGTTCGGGCGACGCGGGCCCGCTGGGCTATGTCGAGGA
GGGTAGCCTGAGGTTATTGAGGAATTGGCCTGAAGCTGACGCAAGCCGGGCGCCGGACGCCACGAAAATAATGGCAGCCC
TCGACGACGCCACGGACTGGCCGCATGTTGAAATCGTCATGAGTCACGCCGGTGCCCGTGGCGCCGTGGTGCAAGCCCTT
ATGCAGCAAGGCGTCCGGGGCCTGGTTGTCGCCACTACGGGCAACGGCACCGTACATCAGGCACTGGAAGCTGCTTTGCT
CAAGGCGCAGGCCGCCGGCGTCAGCGTGGTTCGCGCAACGCGCTGCCCCAATGGCCGGGTGTTGCCCAAACCGGGTGATC
TCATTCCCGATTCGCAGGGGTTGAGTCCGGTGAAGGCGCGGATAGACCTGATGCTTCGTTTGCTGGGCTGA

Upstream 100 bases:

>100_bases
AACCCGGGGTTGTCGCGTGCGTCATCTGTTGTAGTGACCTGTTTTAATATCCAGTAACTGTATTTTCATCCAGTTTTTAA
AAGAACGCAAGTCCCTCCAT

Downstream 100 bases:

>100_bases
GGCCCGGCGCCATGCCGTCTTCAGGATGGCCCGAAGGCCTTTCGGGTCGCCTCAGCTTGACAGGGCCTGGAAGGCTCTTT
CGGTGATCTCATCCACACCG

Product: asparaginase

Products: NA

Alternate protein names: L-ASNase; L-asparagine amidohydrolase [H]

Number of amino acids: Translated: 316; Mature: 316

Protein sequence:

>316_residues
MNRQTIVILGTGGTIAGRAASASDNIGYTAAEVGIEQLVAAIPGLADAGPLIAEQVAQLDSKDMSFAVWAKLAGRVNHFL
AQPDVAGIVVVHGTDTLEETAYFLQAVCGPAKPVVLTCAMRPATALVPDGPQNVLDAVAVARHSGAKGVVAVCAGSVHTA
RDVQKVHPYQVDAFSSGDAGPLGYVEEGSLRLLRNWPEADASRAPDATKIMAALDDATDWPHVEIVMSHAGARGAVVQAL
MQQGVRGLVVATTGNGTVHQALEAALLKAQAAGVSVVRATRCPNGRVLPKPGDLIPDSQGLSPVKARIDLMLRLLG

Sequences:

>Translated_316_residues
MNRQTIVILGTGGTIAGRAASASDNIGYTAAEVGIEQLVAAIPGLADAGPLIAEQVAQLDSKDMSFAVWAKLAGRVNHFL
AQPDVAGIVVVHGTDTLEETAYFLQAVCGPAKPVVLTCAMRPATALVPDGPQNVLDAVAVARHSGAKGVVAVCAGSVHTA
RDVQKVHPYQVDAFSSGDAGPLGYVEEGSLRLLRNWPEADASRAPDATKIMAALDDATDWPHVEIVMSHAGARGAVVQAL
MQQGVRGLVVATTGNGTVHQALEAALLKAQAAGVSVVRATRCPNGRVLPKPGDLIPDSQGLSPVKARIDLMLRLLG
>Mature_316_residues
MNRQTIVILGTGGTIAGRAASASDNIGYTAAEVGIEQLVAAIPGLADAGPLIAEQVAQLDSKDMSFAVWAKLAGRVNHFL
AQPDVAGIVVVHGTDTLEETAYFLQAVCGPAKPVVLTCAMRPATALVPDGPQNVLDAVAVARHSGAKGVVAVCAGSVHTA
RDVQKVHPYQVDAFSSGDAGPLGYVEEGSLRLLRNWPEADASRAPDATKIMAALDDATDWPHVEIVMSHAGARGAVVQAL
MQQGVRGLVVATTGNGTVHQALEAALLKAQAAGVSVVRATRCPNGRVLPKPGDLIPDSQGLSPVKARIDLMLRLLG

Specific function: Unknown

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1789327, Length=298, Percent_Identity=36.5771812080537, Blast_Score=173, Evalue=2e-44,
Organism=Escherichia coli, GI1788065, Length=295, Percent_Identity=27.4576271186441, Blast_Score=81, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI193207372, Length=240, Percent_Identity=28.3333333333333, Blast_Score=68, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17558278, Length=240, Percent_Identity=28.3333333333333, Blast_Score=67, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI193207374, Length=242, Percent_Identity=28.5123966942149, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17558280, Length=242, Percent_Identity=28.5123966942149, Blast_Score=67, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6323189, Length=330, Percent_Identity=30.9090909090909, Blast_Score=135, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6323187, Length=330, Percent_Identity=30.9090909090909, Blast_Score=135, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6323186, Length=330, Percent_Identity=30.9090909090909, Blast_Score=135, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6323184, Length=330, Percent_Identity=30.9090909090909, Blast_Score=135, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6320527, Length=323, Percent_Identity=30.6501547987616, Blast_Score=119, Evalue=5e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004550
- InterPro:   IPR006034
- InterPro:   IPR020827 [H]

Pfam domain/function: PF00710 Asparaginase [H]

EC number: =3.5.1.1 [H]

Molecular weight: Translated: 32554; Mature: 32554

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00144 ASN_GLN_ASE_1 ; PS00917 ASN_GLN_ASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRQTIVILGTGGTIAGRAASASDNIGYTAAEVGIEQLVAAIPGLADAGPLIAEQVAQLD
CCCCEEEEEECCCCEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCC
SKDMSFAVWAKLAGRVNHFLAQPDVAGIVVVHGTDTLEETAYFLQAVCGPAKPVVLTCAM
CCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCEEEEEEC
RPATALVPDGPQNVLDAVAVARHSGAKGVVAVCAGSVHTARDVQKVHPYQVDAFSSGDAG
CCCEEECCCCHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHCCCEEEECCCCCCCC
PLGYVEEGSLRLLRNWPEADASRAPDATKIMAALDDATDWPHVEIVMSHAGARGAVVQAL
CCCCCCCCCCCEECCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHH
MQQGVRGLVVATTGNGTVHQALEAALLKAQAAGVSVVRATRCPNGRVLPKPGDLIPDSQG
HHCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCCCCCCCC
LSPVKARIDLMLRLLG
CCHHHHHHHHHHHHCC
>Mature Secondary Structure
MNRQTIVILGTGGTIAGRAASASDNIGYTAAEVGIEQLVAAIPGLADAGPLIAEQVAQLD
CCCCEEEEEECCCCEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCC
SKDMSFAVWAKLAGRVNHFLAQPDVAGIVVVHGTDTLEETAYFLQAVCGPAKPVVLTCAM
CCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCEEEEEEC
RPATALVPDGPQNVLDAVAVARHSGAKGVVAVCAGSVHTARDVQKVHPYQVDAFSSGDAG
CCCEEECCCCHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHCCCEEEECCCCCCCC
PLGYVEEGSLRLLRNWPEADASRAPDATKIMAALDDATDWPHVEIVMSHAGARGAVVQAL
CCCCCCCCCCCEECCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHH
MQQGVRGLVVATTGNGTVHQALEAALLKAQAAGVSVVRATRCPNGRVLPKPGDLIPDSQG
HHCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCCCCCCCC
LSPVKARIDLMLRLLG
CCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3026924; 3194219; 3379033; 8348975 [H]