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The map label for this gene is thiD [H]

Identifier: 91788200

GI number: 91788200

Start: 2428534

End: 2429400

Strand: Direct

Name: thiD [H]

Synonym: Bpro_2331

Alternate gene names: 91788200

Gene position: 2428534-2429400 (Clockwise)

Preceding gene: 91788192

Following gene: 91788210

Centisome position: 46.7

GC content: 62.86

Gene sequence:

>867_bases
ATGAATCAAGCTTTTGAATTTGAATACCCGCGCGTACTTTCGATTGCGGGTTCTGACAGCAGTGGCGGCGCCGGCATTCA
GGCGGATTTGAAAACCTTTGCCGCGCTGGGGTGCTACGGCATGACGGCGATCACCGCGCTGACCGCTCAAAACACCCTTG
GCGTCAGGGCTATTCACGGCGTTCCACCAGCAATGCTGCGCGACCAGATTGATGCAGTGGTCGAGGACGTGGGTGTGGAC
GCGGTCAAGATTGGCATGTTGCATGCCCCGGATATTGTGCTAACCGTGGCGCAGGCAATCGATCGCCATGGGCTCAAAAA
CGTGGTGCTGGACCCTGTGATGGTCGCGACCAGCGGGGCCATGCTGATCGACAACCCGGCTATTACGGTGCTCGTGAGTG
AGCTGTTTTGGCGTGCCGCCGTGGTCACACCAAATCTGGACGAGGCGTCGTTGCTGGTTGGGCGGCCGCTGGACAGCGAA
CACGCAATGCAGATCGCAGCACAGGAGTTGTTGGCAAAGGGTGCGCAGGCCGTTTTGCTCAAGGGAGGGCATCTGGCCGG
CGAGGTGGTGAGTGATTTATTGGTTACCAGCAGCGGGGAGCTGCACTGGATGCGTGCCCCGCGCATTCATAGCCGCAACA
CGCATGGCACGGGCTGCACGCTATCGTCCGCGATTGCCGCGCATCTGGCGCTGGGGGCCACGTTGCTGGACGCGGTACAG
CTGGCGCGGGCCTATGTGCGCGGGGCGTTGGAAGCGGGTGCCGCTGTGCGCACCGGTGCCGGCAGCGGGCCCCTCAACCA
TGGCTACGCACCCCAAGCCATGCGGCTCAGGCCTCTGCGCCAACCCGTGGCCGGCTCACACGGGTGA

Upstream 100 bases:

>100_bases
CGTCCCACTGCCCTGGTCAGCGCAATACACTTCAGTGGAGTCAGCCCTCCCGGATACAGTGGGCCGGCGGCAAGCGTAAA
AAAGGACCGGAATCAGGAAT

Downstream 100 bases:

>100_bases
GGCTCGCCAGCGCAAACGTCAGCACCAGCGTGGGCAGGGCGGCGCCCCAGCTGGGCACCAGTTGCGCGCAGAGGTGGTAG
CAGGCAATACCCAGCAGCCA

Product: phosphomethylpyrimidine kinase

Products: NA

Alternate protein names: Hydroxymethylpyrimidine kinase; HMP kinase; Hydroxymethylpyrimidine phosphate kinase; HMP-P kinase; HMP-phosphate kinase; HMPP kinase [H]

Number of amino acids: Translated: 288; Mature: 288

Protein sequence:

>288_residues
MNQAFEFEYPRVLSIAGSDSSGGAGIQADLKTFAALGCYGMTAITALTAQNTLGVRAIHGVPPAMLRDQIDAVVEDVGVD
AVKIGMLHAPDIVLTVAQAIDRHGLKNVVLDPVMVATSGAMLIDNPAITVLVSELFWRAAVVTPNLDEASLLVGRPLDSE
HAMQIAAQELLAKGAQAVLLKGGHLAGEVVSDLLVTSSGELHWMRAPRIHSRNTHGTGCTLSSAIAAHLALGATLLDAVQ
LARAYVRGALEAGAAVRTGAGSGPLNHGYAPQAMRLRPLRQPVAGSHG

Sequences:

>Translated_288_residues
MNQAFEFEYPRVLSIAGSDSSGGAGIQADLKTFAALGCYGMTAITALTAQNTLGVRAIHGVPPAMLRDQIDAVVEDVGVD
AVKIGMLHAPDIVLTVAQAIDRHGLKNVVLDPVMVATSGAMLIDNPAITVLVSELFWRAAVVTPNLDEASLLVGRPLDSE
HAMQIAAQELLAKGAQAVLLKGGHLAGEVVSDLLVTSSGELHWMRAPRIHSRNTHGTGCTLSSAIAAHLALGATLLDAVQ
LARAYVRGALEAGAAVRTGAGSGPLNHGYAPQAMRLRPLRQPVAGSHG
>Mature_288_residues
MNQAFEFEYPRVLSIAGSDSSGGAGIQADLKTFAALGCYGMTAITALTAQNTLGVRAIHGVPPAMLRDQIDAVVEDVGVD
AVKIGMLHAPDIVLTVAQAIDRHGLKNVVLDPVMVATSGAMLIDNPAITVLVSELFWRAAVVTPNLDEASLLVGRPLDSE
HAMQIAAQELLAKGAQAVLLKGGHLAGEVVSDLLVTSSGELHWMRAPRIHSRNTHGTGCTLSSAIAAHLALGATLLDAVQ
LARAYVRGALEAGAAVRTGAGSGPLNHGYAPQAMRLRPLRQPVAGSHG

Specific function: Catalyzes the phosphorylation of hydroxymethylpyrimidine phosphate (HMP-P) to HMP-PP, and of HMP to HMP-P. Shows no activity with pyridoxal, pyridoxamine or pyridoxine [H]

COG id: COG0351

COG function: function code H; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiD family [H]

Homologues:

Organism=Escherichia coli, GI1788420, Length=259, Percent_Identity=47.4903474903475, Blast_Score=217, Evalue=5e-58,
Organism=Saccharomyces cerevisiae, GI6325378, Length=273, Percent_Identity=32.2344322344322, Blast_Score=145, Evalue=5e-36,
Organism=Saccharomyces cerevisiae, GI6324997, Length=273, Percent_Identity=35.8974358974359, Blast_Score=127, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6324517, Length=273, Percent_Identity=35.1648351648352, Blast_Score=127, Evalue=2e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013749
- InterPro:   IPR004399 [H]

Pfam domain/function: PF08543 Phos_pyr_kin [H]

EC number: =2.7.1.49; =2.7.4.7 [H]

Molecular weight: Translated: 29827; Mature: 29827

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQAFEFEYPRVLSIAGSDSSGGAGIQADLKTFAALGCYGMTAITALTAQNTLGVRAIHG
CCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECC
VPPAMLRDQIDAVVEDVGVDAVKIGMLHAPDIVLTVAQAIDRHGLKNVVLDPVMVATSGA
CCHHHHHHHHHHHHHHCCCCEEEECCEECCHHHHHHHHHHHHCCCHHHHHCCHHHHCCCC
MLIDNPAITVLVSELFWRAAVVTPNLDEASLLVGRPLDSEHAMQIAAQELLAKGAQAVLL
EEECCCHHHHHHHHHHHHHHEECCCCCCCCEEECCCCCHHHHHHHHHHHHHHCCCCEEEE
KGGHLAGEVVSDLLVTSSGELHWMRAPRIHSRNTHGTGCTLSSAIAAHLALGATLLDAVQ
ECCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
LARAYVRGALEAGAAVRTGAGSGPLNHGYAPQAMRLRPLRQPVAGSHG
HHHHHHHHHHHCCCEEECCCCCCCCCCCCCCHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MNQAFEFEYPRVLSIAGSDSSGGAGIQADLKTFAALGCYGMTAITALTAQNTLGVRAIHG
CCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECC
VPPAMLRDQIDAVVEDVGVDAVKIGMLHAPDIVLTVAQAIDRHGLKNVVLDPVMVATSGA
CCHHHHHHHHHHHHHHCCCCEEEECCEECCHHHHHHHHHHHHCCCHHHHHCCHHHHCCCC
MLIDNPAITVLVSELFWRAAVVTPNLDEASLLVGRPLDSEHAMQIAAQELLAKGAQAVLL
EEECCCHHHHHHHHHHHHHHEECCCCCCCCEEECCCCCHHHHHHHHHHHHHHCCCCEEEE
KGGHLAGEVVSDLLVTSSGELHWMRAPRIHSRNTHGTGCTLSSAIAAHLALGATLLDAVQ
ECCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
LARAYVRGALEAGAAVRTGAGSGPLNHGYAPQAMRLRPLRQPVAGSHG
HHHHHHHHHHHCCCEEECCCCCCCCCCCCCCHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10075431; 9097040; 9278503 [H]