Click here to switch to the map view.

The map label for this gene is leuA

Identifier: 91788191

GI number: 91788191

Start: 2416711

End: 2418252

Strand: Direct

Name: leuA

Synonym: Bpro_2322

Alternate gene names: 91788191

Gene position: 2416711-2418252 (Clockwise)

Preceding gene: 91788190

Following gene: 91788192

Centisome position: 46.47

GC content: 57.78

Gene sequence:

>1542_bases
ATGACCGACAAACTGATCATTTTTGATACCACCTTACGCGATGGCGAGCAGTCGCCCGGCGCGTCCATGACGAAAGATGA
AAAACTGCGCATTGCGCGCCAGCTGGAGCGTCTGAAAGTGGACGTTATCGAGGCCGGATTTGCAGCAAGCTCCAACGGCG
ATTTTGAGGCCGTGCAGGCGATCGCGAATACGATCAGGGATTCCACCATTTGCTCGTTGTCGCGCGCCAATGACCGTGAT
ATTTCTCGCGCCGCTGAAGCACTCAAAGGCGCCAACAACGCCCGTATTCATACCTTCATCGCTACCAGTGCGCTGCACAT
GGAAAAGAAGCTGCGCATGACGCCGGAGCAGGTGCTGGAACAGGCCAGGCTCTCGGTGCGTTTTGCCCGCAATCTGGTGG
CGGATGTCGAGTTCAGTCCCGAGGACGGCTATCGCAGCGACGTTGACTACCTGTGCCGCGTGCTTGAGGCTGTGATTGAC
GAGGGCGCCACGACGATCAATGTGCCAGACACGGTGGGTTATGCGGTGCCTGAGCTTTACGGCCACTTCATCAAAACGTT
GCGCGAACGCGTCCCCAACAGTGACAAGGCTATCTGGTCCGTTCATTGCCACAATGACCTGGGCATGGCGGTTGCCAATT
CACTGGCGGGCGTGATGATCGGCGGCGCGCGGCAAGTGGAATGCACAATCAACGGGCTCGGTGAGCGCGCCGGCAACTGC
TCACTTGAAGAAATTGTCATGGCCGTGAAAACACGCAAGGACTATTTCAATCTTGAGATGAACATTGATCCGTTGCATAT
CGTGGCGGCCAGCCGCATGGTCAGCCAGACCACCGGTTTCGTGGTGCAGCCCAACAAGGCCGTGGTGGGTGCCAACGCAT
TCGCACACGCGTCGGGTATTCACCAGGACGGCATGCTGAAGGCGCGCGAGACGTACGAGATCATGCGTGCCGAAGATGTG
GGCTGGAGTGCCAGCAAAATGGTACTGGGCAAGCTCAGCGGCCGCAATGCCTTCAAGCAACGCTTGCAGGACCTCGGTGT
GGCGCTGGAAAGCGAGGCCGACATCAATACGGCATTTGCCAGATTCAAGGAACTGGCCGACCGGAAAAGCGAGATATTCG
ACGAAGACATCCTGGCGCTGGTCAGTGACGAAAGCATCACTCATCATGACCAGGAGCAGTATGGGTTCGTATCCTTGGCA
CAGCACAGCGAAACCGGTGAACGGCCGCATGCGGTCGTTGTGTTCACCGTGGCCGGCAAGGAAATTCGAGGCGAATCGGA
TGGTAATGGCCCGGTGGATGCTTCGCTCAAGGCCATCGAAACGCATGTGAAGAGCGGAGCGGAGATGGTGCTGTATTCGG
TGAACGCCATCAGCGGATCCACCGAGAGCCAGGGTGAAGTCACCGTGCGGTTACAAAATAACGGACGCGTGGTGAATGGC
GTTGGGGCGGACCCGGATATCGTGGTGGCGTCGGCAAAAGCCTACCTTAGCGCGTTGAACAAGCTTCAGAGCAAGGCTGA
CCGCGTTGCTGCACAGGGTTGA

Upstream 100 bases:

>100_bases
TCTCGGGCCTGCAGCGCAGCAGGGCCGGACAGGGCGCCGCTACGGCTACCATTGCACATTGAGACCTACAGTACAGCGAC
CAGCCCACCAGGAGCTTGCC

Downstream 100 bases:

>100_bases
TCTTCCGTCGTCTGCTTTAAATAATGTTTAAATTCAATGACTTAGATTAATGTTCTAGCGAGAATTTCGATCTGGGATAT
CGAATTCCTCCCGAAACTGA

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase

Number of amino acids: Translated: 513; Mature: 512

Protein sequence:

>513_residues
MTDKLIIFDTTLRDGEQSPGASMTKDEKLRIARQLERLKVDVIEAGFAASSNGDFEAVQAIANTIRDSTICSLSRANDRD
ISRAAEALKGANNARIHTFIATSALHMEKKLRMTPEQVLEQARLSVRFARNLVADVEFSPEDGYRSDVDYLCRVLEAVID
EGATTINVPDTVGYAVPELYGHFIKTLRERVPNSDKAIWSVHCHNDLGMAVANSLAGVMIGGARQVECTINGLGERAGNC
SLEEIVMAVKTRKDYFNLEMNIDPLHIVAASRMVSQTTGFVVQPNKAVVGANAFAHASGIHQDGMLKARETYEIMRAEDV
GWSASKMVLGKLSGRNAFKQRLQDLGVALESEADINTAFARFKELADRKSEIFDEDILALVSDESITHHDQEQYGFVSLA
QHSETGERPHAVVVFTVAGKEIRGESDGNGPVDASLKAIETHVKSGAEMVLYSVNAISGSTESQGEVTVRLQNNGRVVNG
VGADPDIVVASAKAYLSALNKLQSKADRVAAQG

Sequences:

>Translated_513_residues
MTDKLIIFDTTLRDGEQSPGASMTKDEKLRIARQLERLKVDVIEAGFAASSNGDFEAVQAIANTIRDSTICSLSRANDRD
ISRAAEALKGANNARIHTFIATSALHMEKKLRMTPEQVLEQARLSVRFARNLVADVEFSPEDGYRSDVDYLCRVLEAVID
EGATTINVPDTVGYAVPELYGHFIKTLRERVPNSDKAIWSVHCHNDLGMAVANSLAGVMIGGARQVECTINGLGERAGNC
SLEEIVMAVKTRKDYFNLEMNIDPLHIVAASRMVSQTTGFVVQPNKAVVGANAFAHASGIHQDGMLKARETYEIMRAEDV
GWSASKMVLGKLSGRNAFKQRLQDLGVALESEADINTAFARFKELADRKSEIFDEDILALVSDESITHHDQEQYGFVSLA
QHSETGERPHAVVVFTVAGKEIRGESDGNGPVDASLKAIETHVKSGAEMVLYSVNAISGSTESQGEVTVRLQNNGRVVNG
VGADPDIVVASAKAYLSALNKLQSKADRVAAQG
>Mature_512_residues
TDKLIIFDTTLRDGEQSPGASMTKDEKLRIARQLERLKVDVIEAGFAASSNGDFEAVQAIANTIRDSTICSLSRANDRDI
SRAAEALKGANNARIHTFIATSALHMEKKLRMTPEQVLEQARLSVRFARNLVADVEFSPEDGYRSDVDYLCRVLEAVIDE
GATTINVPDTVGYAVPELYGHFIKTLRERVPNSDKAIWSVHCHNDLGMAVANSLAGVMIGGARQVECTINGLGERAGNCS
LEEIVMAVKTRKDYFNLEMNIDPLHIVAASRMVSQTTGFVVQPNKAVVGANAFAHASGIHQDGMLKARETYEIMRAEDVG
WSASKMVLGKLSGRNAFKQRLQDLGVALESEADINTAFARFKELADRKSEIFDEDILALVSDESITHHDQEQYGFVSLAQ
HSETGERPHAVVVFTVAGKEIRGESDGNGPVDASLKAIETHVKSGAEMVLYSVNAISGSTESQGEVTVRLQNNGRVVNGV
GADPDIVVASAKAYLSALNKLQSKADRVAAQG

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate)

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1786261, Length=510, Percent_Identity=48.0392156862745, Blast_Score=440, Evalue=1e-124,
Organism=Saccharomyces cerevisiae, GI6324682, Length=551, Percent_Identity=25.5898366606171, Blast_Score=137, Evalue=3e-33,
Organism=Saccharomyces cerevisiae, GI6324225, Length=546, Percent_Identity=26.007326007326, Blast_Score=136, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6320019, Length=374, Percent_Identity=28.8770053475936, Blast_Score=124, Evalue=3e-29,
Organism=Saccharomyces cerevisiae, GI6320071, Length=382, Percent_Identity=28.2722513089005, Blast_Score=122, Evalue=9e-29,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEU1_POLSJ (Q12B47)

Other databases:

- EMBL:   CP000316
- RefSeq:   YP_549143.1
- ProteinModelPortal:   Q12B47
- SMR:   Q12B47
- STRING:   Q12B47
- GeneID:   4015012
- GenomeReviews:   CP000316_GR
- KEGG:   pol:Bpro_2322
- NMPDR:   fig|296591.1.peg.3754
- eggNOG:   COG0119
- HOGENOM:   HBG701974
- OMA:   DQAIISV
- PhylomeDB:   Q12B47
- ProtClustDB:   PRK00915
- BioCyc:   PSP296591:BPRO_2322-MONOMER
- HAMAP:   MF_01025_B
- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005671
- InterPro:   IPR000891
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00917
- TIGRFAMs:   TIGR00973

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer; SSF110921 2-isopropylmalate_synth_dimer

EC number: =2.3.3.13

Molecular weight: Translated: 55634; Mature: 55503

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1; PS00816 AIPM_HOMOCIT_SYNTH_2; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDKLIIFDTTLRDGEQSPGASMTKDEKLRIARQLERLKVDVIEAGFAASSNGDFEAVQA
CCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
IANTIRDSTICSLSRANDRDISRAAEALKGANNARIHTFIATSALHMEKKLRMTPEQVLE
HHHHHCCHHHEECCCCCCHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHCCCHHHHHH
QARLSVRFARNLVADVEFSPEDGYRSDVDYLCRVLEAVIDEGATTINVPDTVGYAVPELY
HHHHHHHHHHHHHHCCEECCCCCCCCCHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHH
GHFIKTLRERVPNSDKAIWSVHCHNDLGMAVANSLAGVMIGGARQVECTINGLGERAGNC
HHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHEEECCCEEEEEEEECCCCCCCCC
SLEEIVMAVKTRKDYFNLEMNIDPLHIVAASRMVSQTTGFVVQPNKAVVGANAFAHASGI
CHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHCCCEEECCCCEEECCCHHHHHCCC
HQDGMLKARETYEIMRAEDVGWSASKMVLGKLSGRNAFKQRLQDLGVALESEADINTAFA
CCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHCCCCHHHHHHHHHHCCEECCCCCHHHHHH
RFKELADRKSEIFDEDILALVSDESITHHDQEQYGFVSLAQHSETGERPHAVVVFTVAGK
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCHHEEECCCCCCCCCCEEEEEEECCC
EIRGESDGNGPVDASLKAIETHVKSGAEMVLYSVNAISGSTESQGEVTVRLQNNGRVVNG
EECCCCCCCCCCCHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCCEEEEEECCCCEEEEC
VGADPDIVVASAKAYLSALNKLQSKADRVAAQG
CCCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TDKLIIFDTTLRDGEQSPGASMTKDEKLRIARQLERLKVDVIEAGFAASSNGDFEAVQA
CCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
IANTIRDSTICSLSRANDRDISRAAEALKGANNARIHTFIATSALHMEKKLRMTPEQVLE
HHHHHCCHHHEECCCCCCHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHCCCHHHHHH
QARLSVRFARNLVADVEFSPEDGYRSDVDYLCRVLEAVIDEGATTINVPDTVGYAVPELY
HHHHHHHHHHHHHHCCEECCCCCCCCCHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHH
GHFIKTLRERVPNSDKAIWSVHCHNDLGMAVANSLAGVMIGGARQVECTINGLGERAGNC
HHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHEEECCCEEEEEEEECCCCCCCCC
SLEEIVMAVKTRKDYFNLEMNIDPLHIVAASRMVSQTTGFVVQPNKAVVGANAFAHASGI
CHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHCCCEEECCCCEEECCCHHHHHCCC
HQDGMLKARETYEIMRAEDVGWSASKMVLGKLSGRNAFKQRLQDLGVALESEADINTAFA
CCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHCCCCHHHHHHHHHHCCEECCCCCHHHHHH
RFKELADRKSEIFDEDILALVSDESITHHDQEQYGFVSLAQHSETGERPHAVVVFTVAGK
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCHHEEECCCCCCCCCCEEEEEEECCC
EIRGESDGNGPVDASLKAIETHVKSGAEMVLYSVNAISGSTESQGEVTVRLQNNGRVVNG
EECCCCCCCCCCCHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCCEEEEEECCCCEEEEC
VGADPDIVVASAKAYLSALNKLQSKADRVAAQG
CCCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA