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The map label for this gene is rph [H]

Identifier: 91787235

GI number: 91787235

Start: 1379532

End: 1380263

Strand: Direct

Name: rph [H]

Synonym: Bpro_1339

Alternate gene names: 91787235

Gene position: 1379532-1380263 (Clockwise)

Preceding gene: 91787234

Following gene: 91787236

Centisome position: 26.53

GC content: 62.02

Gene sequence:

>732_bases
ATGAGTCAGTTTGTCAGAGTCGACGCGCGTGCTGCCGATGCCCTGCGTCCGGTGCGCATTACCCGCCACTACACCGCCTA
TGCCGAAGGGTCCGTGCTGATTGAATTCGGCGGCACCAAGGTGCTGTGCACAGCCTCCGTCGAGGAGAAAGTACCTGGCC
ACAAAAAAGGCAGCGGCGAGGGCTGGGTGACTGCCGAATACGGCATGCTGCCGCGCGCCACGCATACCCGCAGCGACCGC
GAAGCCGCGCGCGGCAAGCAGAGCGGCCGTACCCAGGAAATCCAGCGGCTGATCGGCCGCTCCATGCGGTCCGTGTTTGA
CCTGAAAAAGCTGGGCGAACGAACCATTTATCTCGATTGCGACGTGATCCAGGCCGATGGTGGCACCCGCACGGCCAGCA
TCACCGGTGCCTTCGTGGCTGTGCAGGATGCGGTCAACCAGCTGCTGGCCCTTGGCAAGCTTCAGGAAACCCCGATCCTC
GATCACGTGGCCGCCATCTCGGTGGGCATTGTGCAGGGCACCCCCCTGCTTGACCTGGAATACACCGAAGACTCGGCCTG
TGACACCGACATGAACGTGGTCATGACCGGCGCAGGCAACTATGTGGAGGTGCAGGGCACGGCGGAAGGCGCCGCTTTTT
CTCGCAAGGAAATGGACGCGCTGCTGGTGCTGGCTGAAAAAGGCATCGCCGAGCTGGTCGGCCTGCAGAAGAAATCGCTA
TCAAATGAATAG

Upstream 100 bases:

>100_bases
TTGAGCGTTCCATCGCGCTCTTTCACGCTTGTTACCTCTTTTTCCTTCCTTTACTGCTTTTTCCATTCGTTCGGCGTTTT
CCGCCAGAAAGCCATCCACC

Downstream 100 bases:

>100_bases
CTTATCAGCTCCGTAAAATAAGAGATAGAGTTACTTTTCATGATGAAAATCGTCCTGGCCTCCAACAACGCCGGCAAGCT
CGCCGAGCTGCAGGCCATGC

Product: ribonuclease PH

Products: NA

Alternate protein names: RNase PH; tRNA nucleotidyltransferase [H]

Number of amino acids: Translated: 243; Mature: 242

Protein sequence:

>243_residues
MSQFVRVDARAADALRPVRITRHYTAYAEGSVLIEFGGTKVLCTASVEEKVPGHKKGSGEGWVTAEYGMLPRATHTRSDR
EAARGKQSGRTQEIQRLIGRSMRSVFDLKKLGERTIYLDCDVIQADGGTRTASITGAFVAVQDAVNQLLALGKLQETPIL
DHVAAISVGIVQGTPLLDLEYTEDSACDTDMNVVMTGAGNYVEVQGTAEGAAFSRKEMDALLVLAEKGIAELVGLQKKSL
SNE

Sequences:

>Translated_243_residues
MSQFVRVDARAADALRPVRITRHYTAYAEGSVLIEFGGTKVLCTASVEEKVPGHKKGSGEGWVTAEYGMLPRATHTRSDR
EAARGKQSGRTQEIQRLIGRSMRSVFDLKKLGERTIYLDCDVIQADGGTRTASITGAFVAVQDAVNQLLALGKLQETPIL
DHVAAISVGIVQGTPLLDLEYTEDSACDTDMNVVMTGAGNYVEVQGTAEGAAFSRKEMDALLVLAEKGIAELVGLQKKSL
SNE
>Mature_242_residues
SQFVRVDARAADALRPVRITRHYTAYAEGSVLIEFGGTKVLCTASVEEKVPGHKKGSGEGWVTAEYGMLPRATHTRSDRE
AARGKQSGRTQEIQRLIGRSMRSVFDLKKLGERTIYLDCDVIQADGGTRTASITGAFVAVQDAVNQLLALGKLQETPILD
HVAAISVGIVQGTPLLDLEYTEDSACDTDMNVVMTGAGNYVEVQGTAEGAAFSRKEMDALLVLAEKGIAELVGLQKKSLS
NE

Specific function: Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates [H]

COG id: COG0689

COG function: function code J; RNase PH

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase PH family [H]

Homologues:

Organism=Escherichia coli, GI157672248, Length=210, Percent_Identity=65.2380952380952, Blast_Score=280, Evalue=9e-77,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR020568
- InterPro:   IPR002381
- InterPro:   IPR018336 [H]

Pfam domain/function: PF01138 RNase_PH; PF03725 RNase_PH_C [H]

EC number: =2.7.7.56 [H]

Molecular weight: Translated: 26131; Mature: 26000

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: PS01277 RIBONUCLEASE_PH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQFVRVDARAADALRPVRITRHYTAYAEGSVLIEFGGTKVLCTASVEEKVPGHKKGSGE
CCCEEEECCHHHHCCCCEEEEEEEEEEECCEEEEEECCCEEEEEECHHHHCCCCCCCCCC
GWVTAEYGMLPRATHTRSDREAARGKQSGRTQEIQRLIGRSMRSVFDLKKLGERTIYLDC
CEEEEECCCCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEEE
DVIQADGGTRTASITGAFVAVQDAVNQLLALGKLQETPILDHVAAISVGIVQGTPLLDLE
EEEECCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHEEEEECCCCEEEEE
YTEDSACDTDMNVVMTGAGNYVEVQGTAEGAAFSRKEMDALLVLAEKGIAELVGLQKKSL
ECCCCCCCCCCEEEEECCCCEEEEEECCCCCHHHHHHHHEEEEEHHCCHHHHHHHHHHHC
SNE
CCC
>Mature Secondary Structure 
SQFVRVDARAADALRPVRITRHYTAYAEGSVLIEFGGTKVLCTASVEEKVPGHKKGSGE
CCEEEECCHHHHCCCCEEEEEEEEEEECCEEEEEECCCEEEEEECHHHHCCCCCCCCCC
GWVTAEYGMLPRATHTRSDREAARGKQSGRTQEIQRLIGRSMRSVFDLKKLGERTIYLDC
CEEEEECCCCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEEE
DVIQADGGTRTASITGAFVAVQDAVNQLLALGKLQETPILDHVAAISVGIVQGTPLLDLE
EEEECCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHEEEEECCCCEEEEE
YTEDSACDTDMNVVMTGAGNYVEVQGTAEGAAFSRKEMDALLVLAEKGIAELVGLQKKSL
ECCCCCCCCCCEEEEECCCCEEEEEECCCCCHHHHHHHHEEEEEHHCCHHHHHHHHHHHC
SNE
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA