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The map label for this gene is yafJ [H]

Identifier: 91787086

GI number: 91787086

Start: 1228901

End: 1229695

Strand: Direct

Name: yafJ [H]

Synonym: Bpro_1189

Alternate gene names: 91787086

Gene position: 1228901-1229695 (Clockwise)

Preceding gene: 91787085

Following gene: 91787094

Centisome position: 23.63

GC content: 67.42

Gene sequence:

>795_bases
ATGTCGGGCCGGCGCTCGACGCAGTTGAACTTCTCGCTTCAGACGCTGGCTGCCCACAGCGCTGGCGGCAGCACGACCCG
CGACGGTTGGGGTGTCGCGTTCTATCAGGGCAACGATGCGGCCCTGTTTCGGGAACCGACGGCAGCGAGCGGCAGTCCCC
TCGTGCGCTTCCTCCAGTCGCAAGGCCCGAGCACGGCGCTGGCCATCTCACACATCCGGCATGCCACGCGCGGGGCGCTC
AATCTGTCAAATACCCAGCCTTTTGCGCGCGAGCTTGCCGGCCGCATGCATGTGTTCGCCCACAACGGGAATCTGGTGGG
GATCGAGCGCGCCGGGAACCTGGCGTTCGATCGCTACCGCCCCGTCGGCACGACCGACTCAGAACACGCCTTCTGCGCGC
TGCTGGAGCGCCTTCACGGCCTGTGGAACCGCGCATCGCCGCCTCCGCTCCCAGAACGCCGCGCGGTTATAGCGGAGTTC
GCCGCCGATCTGCGCAAGCTCGGGCCCGCCAACTTCCTCTACGCCGATGGCGACATCCTGTTCGCCCACGGGCATCGACG
TATCCAGTCCACCACCGGCCGTGTCGCGCCGCCCGGTCTTTGTCTCTGGTCGTGTCACTGCACGGACCCCGGCGAGCCCG
TCCATGCCAGCGGGGTGACGGTCGCACCCGGCTTCCAGGAGATGGTGCTCGTGGCCAGTGTTCCGCTCACGGGCGAGGTA
TGGCGCCCGCTCGAAGAGGGTGAACTGGTGGTGGTGGTGTCGGGTGGAAGACTGCTTGATGACAGCGGACCGTGA

Upstream 100 bases:

>100_bases
CGCATTGAAAGCCTGGTCGAGCTCGTCGTCGCCCAATTGCCAGGACTACCGGCTACCGTGCGCGAGCCATCCCCCGACCC
GGATGTGTGAGCTCCTCGCC

Downstream 100 bases:

>100_bases
TCGGGCCGTTCGCGGACTTTGTTTGCGCTGGCGGGCTATGGGGTTGAATCAAGACTGCGCGAGGCCGGCGGGACTTTGAG
ATTGCCCTCAATGGTCCTTA

Product: class-II glutamine amidotransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 264; Mature: 263

Protein sequence:

>264_residues
MSGRRSTQLNFSLQTLAAHSAGGSTTRDGWGVAFYQGNDAALFREPTAASGSPLVRFLQSQGPSTALAISHIRHATRGAL
NLSNTQPFARELAGRMHVFAHNGNLVGIERAGNLAFDRYRPVGTTDSEHAFCALLERLHGLWNRASPPPLPERRAVIAEF
AADLRKLGPANFLYADGDILFAHGHRRIQSTTGRVAPPGLCLWSCHCTDPGEPVHASGVTVAPGFQEMVLVASVPLTGEV
WRPLEEGELVVVVSGGRLLDDSGP

Sequences:

>Translated_264_residues
MSGRRSTQLNFSLQTLAAHSAGGSTTRDGWGVAFYQGNDAALFREPTAASGSPLVRFLQSQGPSTALAISHIRHATRGAL
NLSNTQPFARELAGRMHVFAHNGNLVGIERAGNLAFDRYRPVGTTDSEHAFCALLERLHGLWNRASPPPLPERRAVIAEF
AADLRKLGPANFLYADGDILFAHGHRRIQSTTGRVAPPGLCLWSCHCTDPGEPVHASGVTVAPGFQEMVLVASVPLTGEV
WRPLEEGELVVVVSGGRLLDDSGP
>Mature_263_residues
SGRRSTQLNFSLQTLAAHSAGGSTTRDGWGVAFYQGNDAALFREPTAASGSPLVRFLQSQGPSTALAISHIRHATRGALN
LSNTQPFARELAGRMHVFAHNGNLVGIERAGNLAFDRYRPVGTTDSEHAFCALLERLHGLWNRASPPPLPERRAVIAEFA
ADLRKLGPANFLYADGDILFAHGHRRIQSTTGRVAPPGLCLWSCHCTDPGEPVHASGVTVAPGFQEMVLVASVPLTGEVW
RPLEEGELVVVVSGGRLLDDSGP

Specific function: Unknown

COG id: COG0121

COG function: function code R; Predicted glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1786417, Length=260, Percent_Identity=33.8461538461538, Blast_Score=136, Evalue=1e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000583
- InterPro:   IPR017932 [H]

Pfam domain/function: PF00310 GATase_2 [H]

EC number: NA

Molecular weight: Translated: 28244; Mature: 28113

Theoretical pI: Translated: 7.70; Mature: 7.70

Prosite motif: PS00237 G_PROTEIN_RECEP_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGRRSTQLNFSLQTLAAHSAGGSTTRDGWGVAFYQGNDAALFREPTAASGSPLVRFLQS
CCCCCCCEEEEEHHHHHHHCCCCCCCCCCCEEEEEECCCEEEEECCCCCCCCHHHHHHHC
QGPSTALAISHIRHATRGALNLSNTQPFARELAGRMHVFAHNGNLVGIERAGNLAFDRYR
CCCCHHHHHHHHHHHHHCCEECCCCCHHHHHHCCCEEEEEECCCEEEEECCCCEEHHCCC
PVGTTDSEHAFCALLERLHGLWNRASPPPLPERRAVIAEFAADLRKLGPANFLYADGDIL
CCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCEE
FAHGHRRIQSTTGRVAPPGLCLWSCHCTDPGEPVHASGVTVAPGFQEMVLVASVPLTGEV
EECCCEEEHHCCCCCCCCCEEEEEEECCCCCCCEECCCEEECCCHHHEEEEECCCCCHHH
WRPLEEGELVVVVSGGRLLDDSGP
HCCCCCCCEEEEEECCEEECCCCC
>Mature Secondary Structure 
SGRRSTQLNFSLQTLAAHSAGGSTTRDGWGVAFYQGNDAALFREPTAASGSPLVRFLQS
CCCCCCEEEEEHHHHHHHCCCCCCCCCCCEEEEEECCCEEEEECCCCCCCCHHHHHHHC
QGPSTALAISHIRHATRGALNLSNTQPFARELAGRMHVFAHNGNLVGIERAGNLAFDRYR
CCCCHHHHHHHHHHHHHCCEECCCCCHHHHHHCCCEEEEEECCCEEEEECCCCEEHHCCC
PVGTTDSEHAFCALLERLHGLWNRASPPPLPERRAVIAEFAADLRKLGPANFLYADGDIL
CCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCEE
FAHGHRRIQSTTGRVAPPGLCLWSCHCTDPGEPVHASGVTVAPGFQEMVLVASVPLTGEV
EECCCEEEHHCCCCCCCCCEEEEEEECCCCCCCEECCCEEECCCHHHEEEEECCCCCHHH
WRPLEEGELVVVVSGGRLLDDSGP
HCCCCCCCEEEEEECCEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7596361; 9278503 [H]