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The map label for this gene is ddl

Identifier: 91786975

GI number: 91786975

Start: 1110286

End: 1111302

Strand: Direct

Name: ddl

Synonym: Bpro_1077

Alternate gene names: 91786975

Gene position: 1110286-1111302 (Clockwise)

Preceding gene: 91786974

Following gene: 91786976

Centisome position: 21.35

GC content: 65.29

Gene sequence:

>1017_bases
GTGAGTAGCAACGCAGACACATCCCAGGCCGTGAGCGCCGGGACAAGCGTGGGGGCCACGTTTGGCAAGGTCGCCGTCCT
CATGGGGGGCATGTCGGCCGAGCGCGAGATCTCCCTGATGTCGGGCCGGGGCGTGTTGCAGGCGCTGCGTTCCCGCGGCG
TGGATGCCCATGCGTTTGACCCGGCCGAGCGCGACATCAGCGAGCTGAAAAAGGACGAGTTTGCGCGCTGCTTCATCGCG
CTGCATGGCCGGTTCGGTGAAGACGGCACGGTGCAGGGCGCGCTGGAGTTGCTGGGCATACCCTACACCGGCTCCGGCGT
CATGGCGTCCAGCATGGCCATTGACAAGGTGATGACCAAGCGGGTCATGCTCGCCGAAGGCCTGCCGACCCCCACCTATG
TGCTGTTGCGCCGGGGCAGCTATGGCAGCGCCGACGTGAGCGCCGTCCCTGACAAGCTGGGCTTGCCGCTGATTGTCAAG
CCCGCACGGGAAGGCTCATCGATCGGGCTGACCAAGGTGACCGAGCGGGCCGGCATGGCAGATGCGGTGGCGCAGGCCGA
AAAGCTGGATGCCGACATCCTGTGCGAGCAGTTCATCAGTGGCGACGAGGTGACCTGCCCGGTACTGGGAACCGGCAGCG
AGGCGCGCGCCCTGCCGGTCATCCGCATCGTGGCGCCCGACGGCAATTACGACTACCAGAACAAGTACTTCACCGACACC
ACGCAGTACCTGGTGCCCTGTGGCCTGCCTGAAGGCGAAGAGGCCGTCATCCAGCAGCTGGTCCTGCAGGCTTACCGCAC
GCTCAACTGCCGCGGCTGGGCACGCGCCGACGTGATGATCGACAAAGTCACGCGCAAGCCCTACCTGCTTGAAATCAATA
CCTCGCCCGGCATGACCGGGCACTCGCTGGTGCCCATGTCTGCACGGGCGGCCGGCATTTCCTATGAAGACCTGTGCATC
GAGGTGCTGAAGACCACCGCACTCGACCATCAGTCGCAAGGGGGAGGCGCTTCTTGA

Upstream 100 bases:

>100_bases
CCATCCTGGATAACGCACTGGGAGGCGATGTCGTGATGTGCATGGGTGCCGGATCGATTGGGCTCGTGCCCGGCAAATTG
TTGGAACTGGAAGGTGCGCA

Downstream 100 bases:

>100_bases
GCCGCACCATGCCACTGCCAACGTCCCTGCCGGCGGACATCAAGCTGATGAACACCTTTTCGGTGTTTTTTGGCCTGGTG
TTTGCGGCCATGGCGCTGGC

Product: D-alanine--D-alanine ligase

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase

Number of amino acids: Translated: 338; Mature: 337

Protein sequence:

>338_residues
MSSNADTSQAVSAGTSVGATFGKVAVLMGGMSAEREISLMSGRGVLQALRSRGVDAHAFDPAERDISELKKDEFARCFIA
LHGRFGEDGTVQGALELLGIPYTGSGVMASSMAIDKVMTKRVMLAEGLPTPTYVLLRRGSYGSADVSAVPDKLGLPLIVK
PAREGSSIGLTKVTERAGMADAVAQAEKLDADILCEQFISGDEVTCPVLGTGSEARALPVIRIVAPDGNYDYQNKYFTDT
TQYLVPCGLPEGEEAVIQQLVLQAYRTLNCRGWARADVMIDKVTRKPYLLEINTSPGMTGHSLVPMSARAAGISYEDLCI
EVLKTTALDHQSQGGGAS

Sequences:

>Translated_338_residues
MSSNADTSQAVSAGTSVGATFGKVAVLMGGMSAEREISLMSGRGVLQALRSRGVDAHAFDPAERDISELKKDEFARCFIA
LHGRFGEDGTVQGALELLGIPYTGSGVMASSMAIDKVMTKRVMLAEGLPTPTYVLLRRGSYGSADVSAVPDKLGLPLIVK
PAREGSSIGLTKVTERAGMADAVAQAEKLDADILCEQFISGDEVTCPVLGTGSEARALPVIRIVAPDGNYDYQNKYFTDT
TQYLVPCGLPEGEEAVIQQLVLQAYRTLNCRGWARADVMIDKVTRKPYLLEINTSPGMTGHSLVPMSARAAGISYEDLCI
EVLKTTALDHQSQGGGAS
>Mature_337_residues
SSNADTSQAVSAGTSVGATFGKVAVLMGGMSAEREISLMSGRGVLQALRSRGVDAHAFDPAERDISELKKDEFARCFIAL
HGRFGEDGTVQGALELLGIPYTGSGVMASSMAIDKVMTKRVMLAEGLPTPTYVLLRRGSYGSADVSAVPDKLGLPLIVKP
AREGSSIGLTKVTERAGMADAVAQAEKLDADILCEQFISGDEVTCPVLGTGSEARALPVIRIVAPDGNYDYQNKYFTDTT
QYLVPCGLPEGEEAVIQQLVLQAYRTLNCRGWARADVMIDKVTRKPYLLEINTSPGMTGHSLVPMSARAAGISYEDLCIE
VLKTTALDHQSQGGGAS

Specific function: Cell wall formation

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1786280, Length=309, Percent_Identity=50.1618122977346, Blast_Score=300, Evalue=1e-82,
Organism=Escherichia coli, GI1786579, Length=346, Percent_Identity=32.9479768786127, Blast_Score=163, Evalue=2e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DDL_POLSJ (Q12EL3)

Other databases:

- EMBL:   CP000316
- RefSeq:   YP_547927.1
- ProteinModelPortal:   Q12EL3
- SMR:   Q12EL3
- STRING:   Q12EL3
- GeneID:   4012084
- GenomeReviews:   CP000316_GR
- KEGG:   pol:Bpro_1077
- NMPDR:   fig|296591.1.peg.3328
- eggNOG:   COG1181
- HOGENOM:   HBG427092
- OMA:   RAFIILH
- PhylomeDB:   Q12EL3
- ProtClustDB:   PRK01372
- BioCyc:   PSP296591:BPRO_1077-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00047
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR01205

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N; SSF52440 PreATP-grasp-like

EC number: =6.3.2.4

Molecular weight: Translated: 35813; Mature: 35682

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: PS50975 ATP_GRASP; PS00843 DALA_DALA_LIGASE_1; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSNADTSQAVSAGTSVGATFGKVAVLMGGMSAEREISLMSGRGVLQALRSRGVDAHAFD
CCCCCCCHHHHHCCCHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHCCCCCCCCC
PAERDISELKKDEFARCFIALHGRFGEDGTVQGALELLGIPYTGSGVMASSMAIDKVMTK
CHHHHHHHHHHHHHHEEEHEEECCCCCCCCHHHHHHHEECCCCCCCHHHHHHHHHHHHHH
RVMLAEGLPTPTYVLLRRGSYGSADVSAVPDKLGLPLIVKPAREGSSIGLTKVTERAGMA
HHHHHCCCCCCEEEEEECCCCCCCCHHCCCHHCCCCEEEECCCCCCCCCHHHHHHHCCHH
DAVAQAEKLDADILCEQFISGDEVTCPVLGTGSEARALPVIRIVAPDGNYDYQNKYFTDT
HHHHHHHHCCHHHHHHHHCCCCCEEEEEECCCCCCCCCEEEEEEECCCCCCCCCCEECCC
TQYLVPCGLPEGEEAVIQQLVLQAYRTLNCRGWARADVMIDKVTRKPYLLEINTSPGMTG
HHEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHEEHHCCCCEEEEEECCCCCCC
HSLVPMSARAAGISYEDLCIEVLKTTALDHQSQGGGAS
CEECCCCHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
SSNADTSQAVSAGTSVGATFGKVAVLMGGMSAEREISLMSGRGVLQALRSRGVDAHAFD
CCCCCCHHHHHCCCHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHCCCCCCCCC
PAERDISELKKDEFARCFIALHGRFGEDGTVQGALELLGIPYTGSGVMASSMAIDKVMTK
CHHHHHHHHHHHHHHEEEHEEECCCCCCCCHHHHHHHEECCCCCCCHHHHHHHHHHHHHH
RVMLAEGLPTPTYVLLRRGSYGSADVSAVPDKLGLPLIVKPAREGSSIGLTKVTERAGMA
HHHHHCCCCCCEEEEEECCCCCCCCHHCCCHHCCCCEEEECCCCCCCCCHHHHHHHCCHH
DAVAQAEKLDADILCEQFISGDEVTCPVLGTGSEARALPVIRIVAPDGNYDYQNKYFTDT
HHHHHHHHCCHHHHHHHHCCCCCEEEEEECCCCCCCCCEEEEEEECCCCCCCCCCEECCC
TQYLVPCGLPEGEEAVIQQLVLQAYRTLNCRGWARADVMIDKVTRKPYLLEINTSPGMTG
HHEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHEEHHCCCCEEEEEECCCCCCC
HSLVPMSARAAGISYEDLCIEVLKTTALDHQSQGGGAS
CEECCCCHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA