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The map label for this gene is maiA [H]

Identifier: 91786891

GI number: 91786891

Start: 1007985

End: 1008635

Strand: Direct

Name: maiA [H]

Synonym: Bpro_0991

Alternate gene names: 91786891

Gene position: 1007985-1008635 (Clockwise)

Preceding gene: 91786890

Following gene: 91786893

Centisome position: 19.38

GC content: 60.52

Gene sequence:

>651_bases
ATGGGCCGCATCCTTCACACCTACTTTCGCAGCTCGGCAGCGTACCGCGTGCGCATCGCGATGAACCTCAAGCGCCTTCA
GGCCGAGCATGTGTTCATTCACCTCAACCGCAATGGCGGCGAACAGTTTGGCGAAGCGTTTCGCGCCATCAACCCGCAGG
CCTTGGTGCCGGTGTTGAGCGAGGAGGGCGTGCAGATCAGTCAGTCACTGGCGATCATGGAATACCTCGACGAGGTGTAT
CCGCAGGTGCCGCTGTTGCCGCCATCGCCGGCTGACCGGGCTTTTGTGCGCCAGTTGTCGCTGGGCATTGCCTGCGACAT
TCACCCGATCAACAACTTGCGGGTGCTGAAGTATCTGACCGGCTCCATGGAGATTTCGGAAGAAAAGAAAAGCGAATGGA
TCGCACACTGGATCACCTTGGGCCTGGAGGCGATCGAAGCGCAAATTCAGCAGCGCGGCCGCACCGGCGCCTTCTGTTTT
GGCGACACGCCGACAATGGCCGACTGCTGCCTGGTGCCGCAGGTTTTCAGCGCCCAGCGTTTCAATGTTGATTTGTCGGG
CTGCCCGACATTGACGGCGATATTCAACACCTGCCAGGCACTGCCTGCTTTCGCTGCGGCGGCTCCTTCACGCCAGCCCG
ACGCCGAGTGA

Upstream 100 bases:

>100_bases
CCGGCACTTCGTCATTGAAAAGGGATGCGTCGTCTGGCAGGGGACCTCTGACGACCTGCGCGGCCAGCCTGAAATCGTGC
ACCAATATCTCGGAGTCTGA

Downstream 100 bases:

>100_bases
GGTGCGGCGGCGAGTCCGCTACTTCAGCTGCGCCAGCTGCTGGTTCGCCATCGTGATCCATTCCTTGAGGTAGATCTGGT
AATGCGCGTCGTCATGTCGC

Product: maleylacetoacetate isomerase

Products: NA

Alternate protein names: MAAI [H]

Number of amino acids: Translated: 216; Mature: 215

Protein sequence:

>216_residues
MGRILHTYFRSSAAYRVRIAMNLKRLQAEHVFIHLNRNGGEQFGEAFRAINPQALVPVLSEEGVQISQSLAIMEYLDEVY
PQVPLLPPSPADRAFVRQLSLGIACDIHPINNLRVLKYLTGSMEISEEKKSEWIAHWITLGLEAIEAQIQQRGRTGAFCF
GDTPTMADCCLVPQVFSAQRFNVDLSGCPTLTAIFNTCQALPAFAAAAPSRQPDAE

Sequences:

>Translated_216_residues
MGRILHTYFRSSAAYRVRIAMNLKRLQAEHVFIHLNRNGGEQFGEAFRAINPQALVPVLSEEGVQISQSLAIMEYLDEVY
PQVPLLPPSPADRAFVRQLSLGIACDIHPINNLRVLKYLTGSMEISEEKKSEWIAHWITLGLEAIEAQIQQRGRTGAFCF
GDTPTMADCCLVPQVFSAQRFNVDLSGCPTLTAIFNTCQALPAFAAAAPSRQPDAE
>Mature_215_residues
GRILHTYFRSSAAYRVRIAMNLKRLQAEHVFIHLNRNGGEQFGEAFRAINPQALVPVLSEEGVQISQSLAIMEYLDEVYP
QVPLLPPSPADRAFVRQLSLGIACDIHPINNLRVLKYLTGSMEISEEKKSEWIAHWITLGLEAIEAQIQQRGRTGAFCFG
DTPTMADCCLVPQVFSAQRFNVDLSGCPTLTAIFNTCQALPAFAAAAPSRQPDAE

Specific function: Forms An Equimolar Complex With The RNA Polymerase Holoenzyme (Rnap) But Not With The Core Enzyme. It Is Synthesized Predominantly When Cells Are Exposed To Amino Acid Starvation, At Which Time It Accounts For Over 50% Of The Total Protein Synthesized. It

COG id: COG0625

COG function: function code O; Glutathione S-transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GST N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI22202624, Length=212, Percent_Identity=46.6981132075472, Blast_Score=182, Evalue=2e-46,
Organism=Homo sapiens, GI22202622, Length=160, Percent_Identity=45.625, Blast_Score=128, Evalue=3e-30,
Organism=Homo sapiens, GI194440732, Length=212, Percent_Identity=36.7924528301887, Blast_Score=127, Evalue=8e-30,
Organism=Caenorhabditis elegans, GI17551302, Length=211, Percent_Identity=39.8104265402844, Blast_Score=152, Evalue=9e-38,
Organism=Caenorhabditis elegans, GI17556142, Length=213, Percent_Identity=35.6807511737089, Blast_Score=128, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI17510461, Length=212, Percent_Identity=35.8490566037736, Blast_Score=127, Evalue=6e-30,
Organism=Drosophila melanogaster, GI24645375, Length=211, Percent_Identity=46.4454976303318, Blast_Score=184, Evalue=5e-47,
Organism=Drosophila melanogaster, GI45553325, Length=211, Percent_Identity=46.4454976303318, Blast_Score=183, Evalue=6e-47,
Organism=Drosophila melanogaster, GI21355859, Length=211, Percent_Identity=46.4454976303318, Blast_Score=183, Evalue=7e-47,
Organism=Drosophila melanogaster, GI21355857, Length=212, Percent_Identity=35.8490566037736, Blast_Score=144, Evalue=4e-35,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1982 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010987
- InterPro:   IPR004045
- InterPro:   IPR017933
- InterPro:   IPR004046
- InterPro:   IPR005955
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00043 GST_C; PF02798 GST_N [H]

EC number: =5.2.1.2 [H]

Molecular weight: Translated: 23932; Mature: 23801

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGRILHTYFRSSAAYRVRIAMNLKRLQAEHVFIHLNRNGGEQFGEAFRAINPQALVPVLS
CCCHHHHHHCCCCEEEEEEEHHHHHHCEEEEEEEEECCCCHHHHHHHHHCCCHHHHHHHC
EEGVQISQSLAIMEYLDEVYPQVPLLPPSPADRAFVRQLSLGIACDIHPINNLRVLKYLT
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCEEEEECCCCCEEEEEHHH
GSMEISEEKKSEWIAHWITLGLEAIEAQIQQRGRTGAFCFGDTPTMADCCLVPQVFSAQR
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHCCHH
FNVDLSGCPTLTAIFNTCQALPAFAAAAPSRQPDAE
EEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
GRILHTYFRSSAAYRVRIAMNLKRLQAEHVFIHLNRNGGEQFGEAFRAINPQALVPVLS
CCHHHHHHCCCCEEEEEEEHHHHHHCEEEEEEEEECCCCHHHHHHHHHCCCHHHHHHHC
EEGVQISQSLAIMEYLDEVYPQVPLLPPSPADRAFVRQLSLGIACDIHPINNLRVLKYLT
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCEEEEECCCCCEEEEEHHH
GSMEISEEKKSEWIAHWITLGLEAIEAQIQQRGRTGAFCFGDTPTMADCCLVPQVFSAQR
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHCCHH
FNVDLSGCPTLTAIFNTCQALPAFAAAAPSRQPDAE
EEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]