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The map label for this gene is ebgR [H]

Identifier: 91212506

GI number: 91212506

Start: 3449265

End: 3450248

Strand: Direct

Name: ebgR [H]

Synonym: UTI89_C3515

Alternate gene names: 91212506

Gene position: 3449265-3450248 (Clockwise)

Preceding gene: 91212505

Following gene: 91212507

Centisome position: 68.09

GC content: 51.32

Gene sequence:

>984_bases
ATGGCAACACTAAAAGACATCGCAATCGAAGCTGGCGTATCCCTGGCGACAGTATCCAGGGTCTTGAATGACGATCCGAC
ATTGAATGTGAAAGAAGAGACGAAACATCGCATTCTCGAGATCGCCGAAAAGCTGGAGTACAAGACCAGTAGCGCCCGTA
AACTCCAGACAGGCGCAGTCAACCAACACCATATTCTGGCTATCTACAGCTACCAACAGGAGCTGGAGATCAACGATCCT
TACTATCTGGCGATCCGCCACGGCATTGAAACTCAGTGCGAAAAGCTGGCGATCGAACTGACCAACTGTTATGAACACAA
CGGCTTACCAGACATTAAAAACGTCACCGGTATTTTAATTGTCGGCAAACCCACGTCCGCCCTGCGCGCCGCCGCCTGTG
CGTTGACCGACAATATCTGTTTTATCGACTTTCACGAACCCGGCAGCGGTTACGATGCGGTGGATATCGATCTGGCACGT
ATCAGTAAAGAAATCATCGATTTCTATATCAATCAGGGCGTTAATCGTATTGGTTTTATTGGCGGTGAAGACTTACCCGG
CAAGGCGGATATTCGTGAGGTCGCCTTTGCGGAATATGGTCGTCTGAAACACGTGGTACGCGAAGAGGATATCTGGCGCG
GCGGTTTTTCCAGTTCGTCGGGCTATGAACTGGCAAAACAGATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTT
GCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGTGGCCTGAACATCCCACAGGATATTTCGCTTAT
CAGCGTTAACGATATCCCCACCGCGCGATTTACCTTTCCGCCGCTCTCCACCGTGCGCATCCATTCCGAAATGATGGGAA
GTCAGGGCGTTAACCTAGTGTATGAAAAAGCCCGCGATGGTCGCGCGCTGCCGCTGTTGGTCTTCGTTCCCAGCAAATTA
AAACTGCGCGGCACGACCCGTTAA

Upstream 100 bases:

>100_bases
CAGCGTCAAAGAAACGCGCTTTATTTACTGAAAACAGGTGACCCGATAAGCACTTCCTCTACAATGGGGGCGCACATCAG
GGAAAGTAAAAAAGGTAAAC

Downstream 100 bases:

>100_bases
ATCCCCTTGCACGCTGTCCGGCAATCGTTTTTGCCGGACAGTGCTGCCGTTTATTTTCGTGATCCAGTTAAAGTAAATGC
ATTTATCCGCTACTTTTTAG

Product: DNA-binding transcriptional repressor EbgR

Products: NA

Alternate protein names: Ebg operon repressor [H]

Number of amino acids: Translated: 327; Mature: 326

Protein sequence:

>327_residues
MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEKLEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDP
YYLAIRHGIETQCEKLAIELTNCYEHNGLPDIKNVTGILIVGKPTSALRAAACALTDNICFIDFHEPGSGYDAVDIDLAR
ISKEIIDFYINQGVNRIGFIGGEDLPGKADIREVAFAEYGRLKHVVREEDIWRGGFSSSSGYELAKQMLAREDYPKALFV
ASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTARFTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKL
KLRGTTR

Sequences:

>Translated_327_residues
MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEKLEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDP
YYLAIRHGIETQCEKLAIELTNCYEHNGLPDIKNVTGILIVGKPTSALRAAACALTDNICFIDFHEPGSGYDAVDIDLAR
ISKEIIDFYINQGVNRIGFIGGEDLPGKADIREVAFAEYGRLKHVVREEDIWRGGFSSSSGYELAKQMLAREDYPKALFV
ASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTARFTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKL
KLRGTTR
>Mature_326_residues
ATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEKLEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPY
YLAIRHGIETQCEKLAIELTNCYEHNGLPDIKNVTGILIVGKPTSALRAAACALTDNICFIDFHEPGSGYDAVDIDLARI
SKEIIDFYINQGVNRIGFIGGEDLPGKADIREVAFAEYGRLKHVVREEDIWRGGFSSSSGYELAKQMLAREDYPKALFVA
SDSIAIGVLRAIHERGLNIPQDISLISVNDIPTARFTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLK
LRGTTR

Specific function: Repressor for beta galactosidase alpha and beta subunits (ebgA and ebgC). Binds lactose as an inducer [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789456, Length=327, Percent_Identity=98.1651376146789, Blast_Score=657, Evalue=0.0,
Organism=Escherichia coli, GI1787948, Length=334, Percent_Identity=27.8443113772455, Blast_Score=95, Evalue=6e-21,
Organism=Escherichia coli, GI1790369, Length=340, Percent_Identity=27.0588235294118, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1788474, Length=355, Percent_Identity=28.7323943661972, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1790194, Length=317, Percent_Identity=25.8675078864353, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1789068, Length=303, Percent_Identity=27.3927392739274, Blast_Score=81, Evalue=8e-17,
Organism=Escherichia coli, GI1787580, Length=337, Percent_Identity=24.0356083086053, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1786540, Length=340, Percent_Identity=25.8823529411765, Blast_Score=79, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36251; Mature: 36120

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEKLEYKTSSARKLQTGAV
CCCHHHHHHHCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHCCCC
NQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTNCYEHNGLPDIKNVTGILI
CCCEEEEEEEECEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEE
VGKPTSALRAAACALTDNICFIDFHEPGSGYDAVDIDLARISKEIIDFYINQGVNRIGFI
ECCCHHHHHHHHHHEECCEEEEEECCCCCCCCEEEHHHHHHHHHHHHHHHHCCCCEEEEC
GGEDLPGKADIREVAFAEYGRLKHVVREEDIWRGGFSSSSGYELAKQMLAREDYPKALFV
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCEEEE
ASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTARFTFPPLSTVRIHSEMMGSQGVNLV
ECCCHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCEEECCCCCCEEHHHHHHCCCCCCEE
YEKARDGRALPLLVFVPSKLKLRGTTR
EECCCCCCCCEEEEECCCCEEECCCCC
>Mature Secondary Structure 
ATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEKLEYKTSSARKLQTGAV
CCHHHHHHHCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHCCCC
NQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTNCYEHNGLPDIKNVTGILI
CCCEEEEEEEECEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEE
VGKPTSALRAAACALTDNICFIDFHEPGSGYDAVDIDLARISKEIIDFYINQGVNRIGFI
ECCCHHHHHHHHHHEECCEEEEEECCCCCCCCEEEHHHHHHHHHHHHHHHHCCCCEEEEC
GGEDLPGKADIREVAFAEYGRLKHVVREEDIWRGGFSSSSGYELAKQMLAREDYPKALFV
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCEEEE
ASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTARFTFPPLSTVRIHSEMMGSQGVNLV
ECCCHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCEEECCCCCCEEHHHHHHCCCCCCEE
YEKARDGRALPLLVFVPSKLKLRGTTR
EECCCCCCCCEEEEECCCCEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2515108; 3939708; 9278503 [H]