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The map label for this gene is ydfI [H]

Identifier: 91212429

GI number: 91212429

Start: 3373026

End: 3374534

Strand: Direct

Name: ydfI [H]

Synonym: UTI89_C3437

Alternate gene names: 91212429

Gene position: 3373026-3374534 (Clockwise)

Preceding gene: 91212428

Following gene: 91212430

Centisome position: 66.59

GC content: 47.71

Gene sequence:

>1509_bases
TTGGTCTACACACAAGTCGTCAGGAGTAAGAGCATAATGTCAGGGAACGAAAGTATCGCTGCCGCCCACTATGATCAGGT
AACCACCTGGCCTCGGGACGGGTTACAGGCAGATATTGTCCATATTGGTTTTGGCGCTTTTCATCGCGGACACCAGGCTG
TCTACACGGATCTCGCTAATCAACTTTCGGACACCCGCTGGGGGATCTTTGAGATCAACCTGTTTGGTGATGCTCAACTG
ATCGAAAACTTAAATGCGCAAAATGGGCTGTTTTCGGTTGTGGAAACATCTGCATCGCAATCTACCTCACGCCTTGTGCG
TTCGGTGGCTGGCGGTATTCATACCCCCAGAGATGGCATTGCCGCAGCAATCCATAAACTGGCTGAACCTCAGGTAAAAA
TCGTTTCATTAACTATCACCGCGAAAGGCTATTGTCTCGATCCGCAAACGCGATCACTTGATCTCACCAATGGATTAATC
AACCACGATCTACAAAATCCGGATGCGCCTCAGTCGGCTATTGGCGTGATCGTCTGTGCTTTGCAACAACGTAAAGCGGC
AGGACTCGCCGCTTTTAGTGTGCTCTCCTGTGACAACTTGCCAGACAATGGGCATCTGACGCGCAATGCCGTTCTCGGTT
TTGCCCGACAACTGGACCAGCCCCTGGCTCAGTGGATCGAAGAAAATGTCTCATTTCCAGGCACGATGGTCGATCGCATT
GTTCCGGCAATGACTGAATCGCAATTCGCCTTACTGGAAACTAAAACCGGTTATGCCGATCCCTGCGGGATCGTCTGCGA
ATCATTTCGTCAGTGGGTTATCGAAGATAATTTTGTGCGCGGACGACCGGAATGGGATAAAGCCGGCGCGATGTTTGTCA
GCAATGTTCAGCCTTATGAAGAGATGAAGTTACGCATGTTAAATGGTAGCCATTCATTTCTGGCTTATAACGGCTCACTG
GCTGGCTATGAGTTTATCTGGCAATGCATGGAAGACGCTAATTTTCGTTCCATTACCCACCAACTGATGATTAATGAACA
AGCCCGAACACTTAATCCAGACTTAAATATCAACATCCAGGAATACGCCGACCTGTTAATTGAACGCTTTAGCAACCGTA
ACGTTGCACACCGTACCGGGCAAATAGCCATGGACGGTTCACAAAAGCTTCCCCAGCGTGCACTGACGCCCTGGCTGAAA
TTGCATCAGCAAAAACAAAACAATGCTGTTCTGTCACTGCTCGTTGCTGGTTGGTTGCATTATGTCATTGATGCTGTTGA
GAAAAGCCAGTCTGTCGCTGATCCAATGAATGACCAATTTCAGGCGCTAATAAAGGAACAACAAGACGCATGGCAACAGG
CGCTCGCATTACTGCACCTTAGCGCCATATTTGGTGATTTAAGCAACCATCAGCCATTTATAAATGAAATAAAAATCGCC
TTTGCGAATATAAAAAACAAAGGCATCAAGGCCACCATCAGCCAATTATTATCGGATGAGCAGAAATGA

Upstream 100 bases:

>100_bases
ATACAGATATAAATAACGCCTTGTTGTTCTGTTTCGTACTTTTACCTTTCTCTGCAAGCGTGATGCATCTCTCTGTTTTT
GGTCTAGTGAATTATGTAAA

Downstream 100 bases:

>100_bases
AAACATTAATTTGTCAGCAGCCTGGCGTTATGGAATATGTGGAAAAGGATATTCCCACACCAGCAGATAATGAAGTGCTG
TTAAAAATCAAAGCTGTGGG

Product: oxidoreductase YdfI

Products: D-fructuronate; NADH [C]

Alternate protein names: NA

Number of amino acids: Translated: 502; Mature: 502

Protein sequence:

>502_residues
MVYTQVVRSKSIMSGNESIAAAHYDQVTTWPRDGLQADIVHIGFGAFHRGHQAVYTDLANQLSDTRWGIFEINLFGDAQL
IENLNAQNGLFSVVETSASQSTSRLVRSVAGGIHTPRDGIAAAIHKLAEPQVKIVSLTITAKGYCLDPQTRSLDLTNGLI
NHDLQNPDAPQSAIGVIVCALQQRKAAGLAAFSVLSCDNLPDNGHLTRNAVLGFARQLDQPLAQWIEENVSFPGTMVDRI
VPAMTESQFALLETKTGYADPCGIVCESFRQWVIEDNFVRGRPEWDKAGAMFVSNVQPYEEMKLRMLNGSHSFLAYNGSL
AGYEFIWQCMEDANFRSITHQLMINEQARTLNPDLNINIQEYADLLIERFSNRNVAHRTGQIAMDGSQKLPQRALTPWLK
LHQQKQNNAVLSLLVAGWLHYVIDAVEKSQSVADPMNDQFQALIKEQQDAWQQALALLHLSAIFGDLSNHQPFINEIKIA
FANIKNKGIKATISQLLSDEQK

Sequences:

>Translated_502_residues
MVYTQVVRSKSIMSGNESIAAAHYDQVTTWPRDGLQADIVHIGFGAFHRGHQAVYTDLANQLSDTRWGIFEINLFGDAQL
IENLNAQNGLFSVVETSASQSTSRLVRSVAGGIHTPRDGIAAAIHKLAEPQVKIVSLTITAKGYCLDPQTRSLDLTNGLI
NHDLQNPDAPQSAIGVIVCALQQRKAAGLAAFSVLSCDNLPDNGHLTRNAVLGFARQLDQPLAQWIEENVSFPGTMVDRI
VPAMTESQFALLETKTGYADPCGIVCESFRQWVIEDNFVRGRPEWDKAGAMFVSNVQPYEEMKLRMLNGSHSFLAYNGSL
AGYEFIWQCMEDANFRSITHQLMINEQARTLNPDLNINIQEYADLLIERFSNRNVAHRTGQIAMDGSQKLPQRALTPWLK
LHQQKQNNAVLSLLVAGWLHYVIDAVEKSQSVADPMNDQFQALIKEQQDAWQQALALLHLSAIFGDLSNHQPFINEIKIA
FANIKNKGIKATISQLLSDEQK
>Mature_502_residues
MVYTQVVRSKSIMSGNESIAAAHYDQVTTWPRDGLQADIVHIGFGAFHRGHQAVYTDLANQLSDTRWGIFEINLFGDAQL
IENLNAQNGLFSVVETSASQSTSRLVRSVAGGIHTPRDGIAAAIHKLAEPQVKIVSLTITAKGYCLDPQTRSLDLTNGLI
NHDLQNPDAPQSAIGVIVCALQQRKAAGLAAFSVLSCDNLPDNGHLTRNAVLGFARQLDQPLAQWIEENVSFPGTMVDRI
VPAMTESQFALLETKTGYADPCGIVCESFRQWVIEDNFVRGRPEWDKAGAMFVSNVQPYEEMKLRMLNGSHSFLAYNGSL
AGYEFIWQCMEDANFRSITHQLMINEQARTLNPDLNINIQEYADLLIERFSNRNVAHRTGQIAMDGSQKLPQRALTPWLK
LHQQKQNNAVLSLLVAGWLHYVIDAVEKSQSVADPMNDQFQALIKEQQDAWQQALALLHLSAIFGDLSNHQPFINEIKIA
FANIKNKGIKATISQLLSDEQK

Specific function: Unknown

COG id: COG0246

COG function: function code G; Mannitol-1-phosphate/altronate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mannitol dehydrogenase family. UxuB subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787823, Length=468, Percent_Identity=49.3589743589744, Blast_Score=449, Evalue=1e-127,
Organism=Escherichia coli, GI1788497, Length=480, Percent_Identity=48.3333333333333, Blast_Score=441, Evalue=1e-125,
Organism=Escherichia coli, GI1790779, Length=474, Percent_Identity=48.9451476793249, Blast_Score=438, Evalue=1e-124,
Organism=Escherichia coli, GI48994885, Length=388, Percent_Identity=24.7422680412371, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1790028, Length=281, Percent_Identity=22.4199288256228, Blast_Score=69, Evalue=8e-13,
Organism=Saccharomyces cerevisiae, GI6324401, Length=479, Percent_Identity=33.6116910229645, Blast_Score=283, Evalue=4e-77,
Organism=Saccharomyces cerevisiae, GI6320765, Length=479, Percent_Identity=33.6116910229645, Blast_Score=283, Evalue=4e-77,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008927
- InterPro:   IPR013328
- InterPro:   IPR000669
- InterPro:   IPR013118
- InterPro:   IPR023027
- InterPro:   IPR013131
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01232 Mannitol_dh; PF08125 Mannitol_dh_C [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 55762; Mature: 55762

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: PS00974 MANNITOL_DHGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVYTQVVRSKSIMSGNESIAAAHYDQVTTWPRDGLQADIVHIGFGAFHRGHQAVYTDLAN
CCHHHHHHHHHHHCCCCCEEEHHHHHCCCCCCCCCCEEEEEECHHHHHCCHHHHHHHHHH
QLSDTRWGIFEINLFGDAQLIENLNAQNGLFSVVETSASQSTSRLVRSVAGGIHTPRDGI
HHCCCCCCEEEEEECCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
AAAIHKLAEPQVKIVSLTITAKGYCLDPQTRSLDLTNGLINHDLQNPDAPQSAIGVIVCA
HHHHHHHCCCCEEEEEEEEEECCEEECCCCCCEEHHHHHHHCCCCCCCCCHHHHHHHHHH
LQQRKAAGLAAFSVLSCDNLPDNGHLTRNAVLGFARQLDQPLAQWIEENVSFPGTMVDRI
HHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
VPAMTESQFALLETKTGYADPCGIVCESFRQWVIEDNFVRGRPEWDKAGAMFVSNVQPYE
HHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCCHH
EMKLRMLNGSHSFLAYNGSLAGYEFIWQCMEDANFRSITHQLMINEQARTLNPDLNINIQ
HHHHHHCCCCCCEEEECCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHCCCCCCEEHH
EYADLLIERFSNRNVAHRTGQIAMDGSQKLPQRALTPWLKLHQQKQNNAVLSLLVAGWLH
HHHHHHHHHHCCCCCHHCCCCEEECCCHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YVIDAVEKSQSVADPMNDQFQALIKEQQDAWQQALALLHLSAIFGDLSNHQPFINEIKIA
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
FANIKNKGIKATISQLLSDEQK
HHHHCCCCHHHHHHHHHCCCCC
>Mature Secondary Structure
MVYTQVVRSKSIMSGNESIAAAHYDQVTTWPRDGLQADIVHIGFGAFHRGHQAVYTDLAN
CCHHHHHHHHHHHCCCCCEEEHHHHHCCCCCCCCCCEEEEEECHHHHHCCHHHHHHHHHH
QLSDTRWGIFEINLFGDAQLIENLNAQNGLFSVVETSASQSTSRLVRSVAGGIHTPRDGI
HHCCCCCCEEEEEECCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
AAAIHKLAEPQVKIVSLTITAKGYCLDPQTRSLDLTNGLINHDLQNPDAPQSAIGVIVCA
HHHHHHHCCCCEEEEEEEEEECCEEECCCCCCEEHHHHHHHCCCCCCCCCHHHHHHHHHH
LQQRKAAGLAAFSVLSCDNLPDNGHLTRNAVLGFARQLDQPLAQWIEENVSFPGTMVDRI
HHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
VPAMTESQFALLETKTGYADPCGIVCESFRQWVIEDNFVRGRPEWDKAGAMFVSNVQPYE
HHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCCHH
EMKLRMLNGSHSFLAYNGSLAGYEFIWQCMEDANFRSITHQLMINEQARTLNPDLNINIQ
HHHHHHCCCCCCEEEECCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHCCCCCCEEHH
EYADLLIERFSNRNVAHRTGQIAMDGSQKLPQRALTPWLKLHQQKQNNAVLSLLVAGWLH
HHHHHHHHHHCCCCCHHCCCCEEECCCHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YVIDAVEKSQSVADPMNDQFQALIKEQQDAWQQALALLHLSAIFGDLSNHQPFINEIKIA
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
FANIKNKGIKATISQLLSDEQK
HHHHCCCCHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: D-mannonate; NAD(+) [C]

Specific reaction: D-mannonate + NAD(+) = D-fructuronate + NADH [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]