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The map label for this gene is kpsF [H]

Identifier: 91212354

GI number: 91212354

Start: 3289151

End: 3290170

Strand: Direct

Name: kpsF [H]

Synonym: UTI89_C3362

Alternate gene names: 91212354

Gene position: 3289151-3290170 (Clockwise)

Preceding gene: 91212352

Following gene: 91212355

Centisome position: 64.93

GC content: 48.82

Gene sequence:

>1020_bases
GTGACTGTGGCATTATTTCCGTGCAAAGGAGCTGATATGTCTGAAAGACATTTACCTGATGACCAGAGCAGTACTATCGA
TCCATATCTAATTACCTCTGTTCGCCAGACTCTGGCAGAACAAGGCGCAGCATTACAAAACTTGTCTAAACAACTGGATT
CCGGGCAGTACCAGCGTGTCCTTAATTTGATAATGAACTGTAAAGGGCACGTTATTCTTTCGGGAATGGGTAAATCAGGG
CATGTCGGTCGTAAAATGTCAGCGACGCTGGCCTCTACGGGTACGCCTAGTTTCTTTATTCATCCTGCAGAAGCTTTCCA
TGGCGATCTGGGCATGATTACGCCTTACGATCTTCTGATCCTTATTTCTGCCAGCGGTGAAACGGATGAAATCCTCAAGC
TAGTTCCTTCACTGAAAAATTTCGGCAACCGAATTATCGCCATTACCAATAATGGAAATTCCACGCTGGCGAAAAATGCT
GATGCCGTGCTGGAACTCCATATGGCGAATGAAACCTGCCCGAATAATCTTGCACCAACAACGTCTACCACGCTGACGAT
GGCGATCGGCGATGCGCTGGCGATTGCCATGATCCGCCAACGCAAATTTATGCCGAATGATTTTGCGCGCTATCACCCGG
GCGGTTCATTAGGTCGTCGCCTGCTGACCCGCGTTGCTGATGTCATGCAACATGATGTTCCTGCGGTACAGCTGGATGCG
TCATTTAAAACCGTGATTCAACGTATCACCAGCGGATGCCAGGGAATGGTGATGGTAGAAGACGCAGAAGGTGGGCTAGC
GGGCATTATCACCGACGGTGACCTGCGTCGCTTTATGGAAAAAGAGGATTCTCTGACATCCGCCACGGCTGCGCAGATGA
TGACACGTGAACCGCTGACGCTACCGGAAGACACCATGATCATTGAAGCGGAAGAAAAAATGCAAAAACACCGTGTCTCA
ACGTTATTGGTGACCAACAAGGCAAATAAAGTCACTGGCCTTGTGCGCATTTTCGACTAA

Upstream 100 bases:

>100_bases
TGCATATAAGCATGGACTGACCATGGTTTTATATTCATTAAAAATTTTGTTACAACCCATTGATTTAGCATAAATAAATT
ATAGTGGGTTCGGGTTTGTT

Downstream 100 bases:

>100_bases
TTAAGCAACGGGGTTCGAGAGGTTAGTCTCTCATTGAGTTTGCGCCCCACTGAAACTTTGATAATCGTTACATGTTGATA
AAAGTGAAGTCTGCCGTATC

Product: polysialic acid capsule synthesis protein KpsF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 339; Mature: 338

Protein sequence:

>339_residues
MTVALFPCKGADMSERHLPDDQSSTIDPYLITSVRQTLAEQGAALQNLSKQLDSGQYQRVLNLIMNCKGHVILSGMGKSG
HVGRKMSATLASTGTPSFFIHPAEAFHGDLGMITPYDLLILISASGETDEILKLVPSLKNFGNRIIAITNNGNSTLAKNA
DAVLELHMANETCPNNLAPTTSTTLTMAIGDALAIAMIRQRKFMPNDFARYHPGGSLGRRLLTRVADVMQHDVPAVQLDA
SFKTVIQRITSGCQGMVMVEDAEGGLAGIITDGDLRRFMEKEDSLTSATAAQMMTREPLTLPEDTMIIEAEEKMQKHRVS
TLLVTNKANKVTGLVRIFD

Sequences:

>Translated_339_residues
MTVALFPCKGADMSERHLPDDQSSTIDPYLITSVRQTLAEQGAALQNLSKQLDSGQYQRVLNLIMNCKGHVILSGMGKSG
HVGRKMSATLASTGTPSFFIHPAEAFHGDLGMITPYDLLILISASGETDEILKLVPSLKNFGNRIIAITNNGNSTLAKNA
DAVLELHMANETCPNNLAPTTSTTLTMAIGDALAIAMIRQRKFMPNDFARYHPGGSLGRRLLTRVADVMQHDVPAVQLDA
SFKTVIQRITSGCQGMVMVEDAEGGLAGIITDGDLRRFMEKEDSLTSATAAQMMTREPLTLPEDTMIIEAEEKMQKHRVS
TLLVTNKANKVTGLVRIFD
>Mature_338_residues
TVALFPCKGADMSERHLPDDQSSTIDPYLITSVRQTLAEQGAALQNLSKQLDSGQYQRVLNLIMNCKGHVILSGMGKSGH
VGRKMSATLASTGTPSFFIHPAEAFHGDLGMITPYDLLILISASGETDEILKLVPSLKNFGNRIIAITNNGNSTLAKNAD
AVLELHMANETCPNNLAPTTSTTLTMAIGDALAIAMIRQRKFMPNDFARYHPGGSLGRRLLTRVADVMQHDVPAVQLDAS
FKTVIQRITSGCQGMVMVEDAEGGLAGIITDGDLRRFMEKEDSLTSATAAQMMTREPLTLPEDTMIIEAEEKMQKHRVST
LLVTNKANKVTGLVRIFD

Specific function: Unknown

COG id: COG0794

COG function: function code M; Predicted sugar phosphate isomerase involved in capsule formation

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SIS domain [H]

Homologues:

Organism=Escherichia coli, GI1789588, Length=307, Percent_Identity=41.042345276873, Blast_Score=237, Evalue=6e-64,
Organism=Escherichia coli, GI87082151, Length=312, Percent_Identity=40.7051282051282, Blast_Score=233, Evalue=2e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR000644
- InterPro:   IPR004800
- InterPro:   IPR001347 [H]

Pfam domain/function: PF00571 CBS; PF01380 SIS [H]

EC number: NA

Molecular weight: Translated: 36787; Mature: 36656

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
5.3 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVALFPCKGADMSERHLPDDQSSTIDPYLITSVRQTLAEQGAALQNLSKQLDSGQYQRV
CEEEEEECCCCCCCHHCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHH
LNLIMNCKGHVILSGMGKSGHVGRKMSATLASTGTPSFFIHPAEAFHGDLGMITPYDLLI
HHHHHCCCCCEEEEECCCCCCCCCHHHHHHHCCCCCCEEEECHHHHCCCCCCCCCEEEEE
LISASGETDEILKLVPSLKNFGNRIIAITNNGNSTLAKNADAVLELHMANETCPNNLAPT
EEECCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCEEEEEECCCCCCCCCCCC
TSTTLTMAIGDALAIAMIRQRKFMPNDFARYHPGGSLGRRLLTRVADVMQHDVPAVQLDA
CCCEEEEHHHHHHHHHHHHHHCCCCCHHHHCCCCHHHHHHHHHHHHHHHHCCCCEEEECC
SFKTVIQRITSGCQGMVMVEDAEGGLAGIITDGDLRRFMEKEDSLTSATAAQMMTREPLT
HHHHHHHHHHCCCCEEEEEEECCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHCCCCC
LPEDTMIIEAEEKMQKHRVSTLLVTNKANKVTGLVRIFD
CCCCCEEEEHHHHHHHHHHHEEEEECCCCCEEEEEEEEC
>Mature Secondary Structure 
TVALFPCKGADMSERHLPDDQSSTIDPYLITSVRQTLAEQGAALQNLSKQLDSGQYQRV
EEEEEECCCCCCCHHCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHH
LNLIMNCKGHVILSGMGKSGHVGRKMSATLASTGTPSFFIHPAEAFHGDLGMITPYDLLI
HHHHHCCCCCEEEEECCCCCCCCCHHHHHHHCCCCCCEEEECHHHHCCCCCCCCCEEEEE
LISASGETDEILKLVPSLKNFGNRIIAITNNGNSTLAKNADAVLELHMANETCPNNLAPT
EEECCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCEEEEEECCCCCCCCCCCC
TSTTLTMAIGDALAIAMIRQRKFMPNDFARYHPGGSLGRRLLTRVADVMQHDVPAVQLDA
CCCEEEEHHHHHHHHHHHHHHCCCCCHHHHCCCCHHHHHHHHHHHHHHHHCCCCEEEECC
SFKTVIQRITSGCQGMVMVEDAEGGLAGIITDGDLRRFMEKEDSLTSATAAQMMTREPLT
HHHHHHHHHHCCCCEEEEEEECCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHCCCCC
LPEDTMIIEAEEKMQKHRVSTLLVTNKANKVTGLVRIFD
CCCCCEEEEHHHHHHHHHHHEEEEECCCCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8932302 [H]