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The map label for this gene is pulE [H]

Identifier: 89885886

GI number: 89885886

Start: 164830

End: 166557

Strand: Reverse

Name: pulE [H]

Synonym: Rfer_4400

Alternate gene names: 89885886

Gene position: 166557-164830 (Counterclockwise)

Preceding gene: 89885887

Following gene: 89885885

Centisome position: 64.7

GC content: 54.75

Gene sequence:

>1728_bases
ATGAGCAACGCCCTGCATATTGTCGAAACGCCAGCGCTGGCTGTGCCATTGCGCACGGGGGAGATTTTGATCCGGGACGG
GGTCATTACGCGCCATCAATTGGAGATTGCGCTAAAACAACAACGGGTATGGCGAAACTCCGGAAGAATTACGCCAATCG
GCGAAATTCTGCACGAGTTGCGTTTTGCCGATCGCGCCGCCATCGAAAATGCGGTATTGATGACAGCCGGCTCCACGACT
GGCATTTTTGAGGTCTTGCTGTCATCCGTCACCTGTATGCGCTATCGCGTGCACCCCATTCGCATGGATGGAGAAACGTT
GGTCATTCAGTCTAGGCAGCGTCTGAGTGATCGGGAAAAGCAGCGCATTCTGGATGCATGCCATGTGTCGGCCAAGTACC
TGAAAGTCGTTGCAGCTGATCACCAGGCCATAGCTTCGGCGCTTTCGATGCATGAAAAGCAGGATTCTTCCCTCGACATC
TGGATCAGACGCTTGCAGCACGACCCATCGGGCTATGTGCTCAAAGCGTTCATGGGGCACATGATCGGGGAGGCATCTCA
ACGGGATGCCTCCGATATCCATCTAGACCGCAAGGACGACGTCCACTCGTGGATTTCCTACCGCATAGATGGCGATATGA
GCCAGATGTACATGCTGCCGTCCAAATTGATGGCCGCCATCATCGTGCGCATCAAAACGGAAGCGGGCATGGACGCATCA
TCTACCCAGCGTCCCCAGGACGGGCGCATCAGTCTTGATTCGCGCCACCAAAAACTTGACATCCGGGTCTCCTCGCAACC
CATCGCAGGGGGTGAGACGATTGCACTTCGACTGCTCAGCTCGGACTCCATCAAGTCTTTGAACATCTTGTTTCCGCAGC
AGCCTGAACTGATTGCGCACCTCAAGAAGCTGACCCGTGTTGATTCAAAGACGGGTGGGTTCATTTTGATTTCCGGCGCG
ACTGGAATGGGGAAGTCCACCACGTTATATGCGTTGTTGCAAGAACTCGACAGGGACCGACTCAACGTCATGACGGTTGA
AGACCCTGTGGAATATCAGTTGGCGTTTCTCAGGCAGATCCAGCTCAATCAGTTTTTGGGCGAGCAAGCTACGGATATGG
AGAGATCACTATTGCGTCAGGACCCGGATGTGCTCGTATTTGGCGAAATGCGCGACGAGAACTCGACAATTGCCGCATTG
CGCCTGGCTGAATCGGGCCACCTGGTGATCTCTACCATTCACGCCAATGACGCGGTACAGACGTTCGAGCGGCTTGCCAA
TAAAGTGGGCGAAGCAAACCGCGATGACATGCTCTACATCCTGGGCAGCTATCTCAAGGCGATTATTGCCCAGCGCCTGA
TCAAGAAGCTGTGCCTTTGCGCGCGCCCCGCCACACCTGAATATCGTTCGCTTCATGCACAGACCGCGGCGGATTTGGGC
ATCAGCGATGAAGCCACTGGTTTGCTGGAGCCAGGAAAATGCAAGGCATGCAACAACACGGGGTACCGGGGGCGCGTCAT
GCTGCACGAGACCTTGCTGATACCCGATGACGATCTTGTTCGCAAAAATTTGCTCACGGCGATCACGTCCCCTAACGGTT
TCTCATCAATCTTGGACGTACCGGGTGTGAAACACATTGCCCGCACGGATACCCTGCGCACGCTGGTTTCATCCGGTGTG
ATTCCTCTGGGGTTTGCAGCGCTTCAAATGGGGGTATCAGCATCATGA

Upstream 100 bases:

>100_bases
CATTTGCCAGTCGCGCCGCACCGGACACAGCGACGGGCAGCTCCACTGGTGCTGCGCCAAGCTCAGATCCGATGCAGCCG
CATGCACCTCAGTTCGCGCG

Downstream 100 bases:

>100_bases
TCAACCCTCAGAGTCAGCAAGTGTGGGCGGCACCCCGCAGACAACTTTTGCGCTGGACCATTTCGTACGCTCCATGGAAC
AGCCCGAAGACCGTCGTCAC

Product: type II secretion system protein E

Products: NA

Alternate protein names: Pullulanase secretion protein pulE; Type II traffic warden ATPase [H]

Number of amino acids: Translated: 575; Mature: 574

Protein sequence:

>575_residues
MSNALHIVETPALAVPLRTGEILIRDGVITRHQLEIALKQQRVWRNSGRITPIGEILHELRFADRAAIENAVLMTAGSTT
GIFEVLLSSVTCMRYRVHPIRMDGETLVIQSRQRLSDREKQRILDACHVSAKYLKVVAADHQAIASALSMHEKQDSSLDI
WIRRLQHDPSGYVLKAFMGHMIGEASQRDASDIHLDRKDDVHSWISYRIDGDMSQMYMLPSKLMAAIIVRIKTEAGMDAS
STQRPQDGRISLDSRHQKLDIRVSSQPIAGGETIALRLLSSDSIKSLNILFPQQPELIAHLKKLTRVDSKTGGFILISGA
TGMGKSTTLYALLQELDRDRLNVMTVEDPVEYQLAFLRQIQLNQFLGEQATDMERSLLRQDPDVLVFGEMRDENSTIAAL
RLAESGHLVISTIHANDAVQTFERLANKVGEANRDDMLYILGSYLKAIIAQRLIKKLCLCARPATPEYRSLHAQTAADLG
ISDEATGLLEPGKCKACNNTGYRGRVMLHETLLIPDDDLVRKNLLTAITSPNGFSSILDVPGVKHIARTDTLRTLVSSGV
IPLGFAALQMGVSAS

Sequences:

>Translated_575_residues
MSNALHIVETPALAVPLRTGEILIRDGVITRHQLEIALKQQRVWRNSGRITPIGEILHELRFADRAAIENAVLMTAGSTT
GIFEVLLSSVTCMRYRVHPIRMDGETLVIQSRQRLSDREKQRILDACHVSAKYLKVVAADHQAIASALSMHEKQDSSLDI
WIRRLQHDPSGYVLKAFMGHMIGEASQRDASDIHLDRKDDVHSWISYRIDGDMSQMYMLPSKLMAAIIVRIKTEAGMDAS
STQRPQDGRISLDSRHQKLDIRVSSQPIAGGETIALRLLSSDSIKSLNILFPQQPELIAHLKKLTRVDSKTGGFILISGA
TGMGKSTTLYALLQELDRDRLNVMTVEDPVEYQLAFLRQIQLNQFLGEQATDMERSLLRQDPDVLVFGEMRDENSTIAAL
RLAESGHLVISTIHANDAVQTFERLANKVGEANRDDMLYILGSYLKAIIAQRLIKKLCLCARPATPEYRSLHAQTAADLG
ISDEATGLLEPGKCKACNNTGYRGRVMLHETLLIPDDDLVRKNLLTAITSPNGFSSILDVPGVKHIARTDTLRTLVSSGV
IPLGFAALQMGVSAS
>Mature_574_residues
SNALHIVETPALAVPLRTGEILIRDGVITRHQLEIALKQQRVWRNSGRITPIGEILHELRFADRAAIENAVLMTAGSTTG
IFEVLLSSVTCMRYRVHPIRMDGETLVIQSRQRLSDREKQRILDACHVSAKYLKVVAADHQAIASALSMHEKQDSSLDIW
IRRLQHDPSGYVLKAFMGHMIGEASQRDASDIHLDRKDDVHSWISYRIDGDMSQMYMLPSKLMAAIIVRIKTEAGMDASS
TQRPQDGRISLDSRHQKLDIRVSSQPIAGGETIALRLLSSDSIKSLNILFPQQPELIAHLKKLTRVDSKTGGFILISGAT
GMGKSTTLYALLQELDRDRLNVMTVEDPVEYQLAFLRQIQLNQFLGEQATDMERSLLRQDPDVLVFGEMRDENSTIAALR
LAESGHLVISTIHANDAVQTFERLANKVGEANRDDMLYILGSYLKAIIAQRLIKKLCLCARPATPEYRSLHAQTAADLGI
SDEATGLLEPGKCKACNNTGYRGRVMLHETLLIPDDDLVRKNLLTAITSPNGFSSILDVPGVKHIARTDTLRTLVSSGVI
PLGFAALQMGVSAS

Specific function: Involved in a general secretion pathway (GSP) for the export of proteins. Required for the translocation of pullulanase [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=380, Percent_Identity=36.3157894736842, Blast_Score=200, Evalue=2e-52,
Organism=Escherichia coli, GI1786296, Length=477, Percent_Identity=29.979035639413, Blast_Score=177, Evalue=1e-45,
Organism=Escherichia coli, GI87082188, Length=188, Percent_Identity=36.1702127659575, Blast_Score=102, Evalue=6e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013369
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 63687; Mature: 63556

Theoretical pI: Translated: 7.72; Mature: 7.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNALHIVETPALAVPLRTGEILIRDGVITRHQLEIALKQQRVWRNSGRITPIGEILHEL
CCCCEEEEECCCEEEEECCCCEEEECCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
RFADRAAIENAVLMTAGSTTGIFEVLLSSVTCMRYRVHPIRMDGETLVIQSRQRLSDREK
HHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHHEEEEEEEECCCEEEEECHHHHHHHHH
QRILDACHVSAKYLKVVAADHQAIASALSMHEKQDSSLDIWIRRLQHDPSGYVLKAFMGH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHH
MIGEASQRDASDIHLDRKDDVHSWISYRIDGDMSQMYMLPSKLMAAIIVRIKTEAGMDAS
HHCCCCCCCCCCCCCCCCCCHHHHHHEEECCCHHHHHHHHHHHHHHHHHHEECCCCCCCC
STQRPQDGRISLDSRHQKLDIRVSSQPIAGGETIALRLLSSDSIKSLNILFPQQPELIAH
CCCCCCCCCEEECCCCCEEEEEECCCCCCCCCEEEEEEECCCCCCEEEEECCCCCHHHHH
LKKLTRVDSKTGGFILISGATGMGKSTTLYALLQELDRDRLNVMTVEDPVEYQLAFLRQI
HHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHH
QLNQFLGEQATDMERSLLRQDPDVLVFGEMRDENSTIAALRLAESGHLVISTIHANDAVQ
HHHHHHCCHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEEEEECCCCEEEEEEECCHHHH
TFERLANKVGEANRDDMLYILGSYLKAIIAQRLIKKLCLCARPATPEYRSLHAQTAADLG
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCC
ISDEATGLLEPGKCKACNNTGYRGRVMLHETLLIPDDDLVRKNLLTAITSPNGFSSILDV
CCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCHHHHHHHHHHHHCCCCCCHHHHCC
PGVKHIARTDTLRTLVSSGVIPLGFAALQMGVSAS
CCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SNALHIVETPALAVPLRTGEILIRDGVITRHQLEIALKQQRVWRNSGRITPIGEILHEL
CCCEEEEECCCEEEEECCCCEEEECCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
RFADRAAIENAVLMTAGSTTGIFEVLLSSVTCMRYRVHPIRMDGETLVIQSRQRLSDREK
HHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHHEEEEEEEECCCEEEEECHHHHHHHHH
QRILDACHVSAKYLKVVAADHQAIASALSMHEKQDSSLDIWIRRLQHDPSGYVLKAFMGH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHH
MIGEASQRDASDIHLDRKDDVHSWISYRIDGDMSQMYMLPSKLMAAIIVRIKTEAGMDAS
HHCCCCCCCCCCCCCCCCCCHHHHHHEEECCCHHHHHHHHHHHHHHHHHHEECCCCCCCC
STQRPQDGRISLDSRHQKLDIRVSSQPIAGGETIALRLLSSDSIKSLNILFPQQPELIAH
CCCCCCCCCEEECCCCCEEEEEECCCCCCCCCEEEEEEECCCCCCEEEEECCCCCHHHHH
LKKLTRVDSKTGGFILISGATGMGKSTTLYALLQELDRDRLNVMTVEDPVEYQLAFLRQI
HHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHH
QLNQFLGEQATDMERSLLRQDPDVLVFGEMRDENSTIAALRLAESGHLVISTIHANDAVQ
HHHHHHCCHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEEEEECCCCEEEEEEECCHHHH
TFERLANKVGEANRDDMLYILGSYLKAIIAQRLIKKLCLCARPATPEYRSLHAQTAADLG
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCC
ISDEATGLLEPGKCKACNNTGYRGRVMLHETLLIPDDDLVRKNLLTAITSPNGFSSILDV
CCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCHHHHHHHHHHHHCCCCCCHHHHCC
PGVKHIARTDTLRTLVSSGVIPLGFAALQMGVSAS
CCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1738317; 2677007 [H]