Click here to switch to the map view.

The map label for this gene is gshB [H]

Identifier: 89256593

GI number: 89256593

Start: 1224237

End: 1225211

Strand: Reverse

Name: gshB [H]

Synonym: FTL_1284

Alternate gene names: 89256593

Gene position: 1225211-1224237 (Counterclockwise)

Preceding gene: 89256594

Following gene: 89256592

Centisome position: 64.62

GC content: 32.1

Gene sequence:

>975_bases
ATGAAAGTTGGATTTATAATAGATAACTTAAACTCTTTTAATATTTCAAAAGATAGCACTTATATGATGCTACATGCAGC
CCAAGATAAAGGCTGGGAGATTTATACCTTTTATCTTAATGATTTATCAATAATTAATGGTAAGCCAAAAGGTGATGCAC
TTAAGATAAAAATTCATAAGACAAAACAAGATTGGTATGAGATTTTATCGCAACGCCATGATTTTTCATTACTAGATCTA
GATTGTATCTTTATGCGTAAAGATCCACCATTTAATATGGAATATATTTATGTAACTTATATGCTTGATTTAGCTAAAAA
AAATGATGTTTTGGTAGTCAATAATCCCCAAGCACTTAGAGATTTTAACGAAAAAGTAGCTATTTCAAATTATCCCAAAT
TTGCGCCGCATACCTTGATCACCAGAAGCTATAAACAGATTAATGAGTTTTATGAAAAGCATAAAGATATTATTGTCAAA
CCTTTAGATGGTATGGGTGGTAGTTCTATCTTTAGAATTAAAGAAGGTGATAAAAACAAAAACGTAATATTAGAAATACT
TACTCAGCATCAAAGCCGTTATATAATGGTGCAAGATTATCAAAAAGCTATCAAAGAGGGTGATAAGAGGATACTTATAG
TTAATGGTGAGCCAATTAAGTATCTTTTAGCAAGAGTGCCTAGTGATAGCGATAATCGTGGCAATCTTGCAGCTGGGGCT
ACAGCAGAAGTTAGAGAACTCCAAGATAGTGATTATAAGATAGCCAAAAAGGTCGCTAAGAAGCTCAAAAAAGAAGGCGT
GATGTTTGCTGGTATTGATGTGATTGGCGATAAATTAACAGAAGTTAATATTACTAGTCCAACAGGTATCCAAGAGATAT
ACAAAGCTACAAAAATAAACGCTGCTAGTTTACTAATGCAAGCAGTTGAGAAAAAAATAAATAAGATGAGACAGGAACAC
GAAAATGGAGAATAA

Upstream 100 bases:

>100_bases
TAGACAATTACAATTTCCTAATAAAAAAATGCTTAATATTGTAGATATTTTAAATGGAAAAGATTTAGATAAATATATTG
GATACAAACTAGGATAAATA

Downstream 100 bases:

>100_bases
ATCAAACTCTCAAATTTTATTTGCAGAAGCACAGCAATATATACCAGGTGGAGTAAACTCTCCAGTTAGGGCATTTAAGA
GTGTTGGACAAGAATTTCCA

Product: glutathione synthetase

Products: NA

Alternate protein names: GSH synthetase; GSH-S; GSHase; Glutathione synthase [H]

Number of amino acids: Translated: 324; Mature: 324

Protein sequence:

>324_residues
MKVGFIIDNLNSFNISKDSTYMMLHAAQDKGWEIYTFYLNDLSIINGKPKGDALKIKIHKTKQDWYEILSQRHDFSLLDL
DCIFMRKDPPFNMEYIYVTYMLDLAKKNDVLVVNNPQALRDFNEKVAISNYPKFAPHTLITRSYKQINEFYEKHKDIIVK
PLDGMGGSSIFRIKEGDKNKNVILEILTQHQSRYIMVQDYQKAIKEGDKRILIVNGEPIKYLLARVPSDSDNRGNLAAGA
TAEVRELQDSDYKIAKKVAKKLKKEGVMFAGIDVIGDKLTEVNITSPTGIQEIYKATKINAASLLMQAVEKKINKMRQEH
ENGE

Sequences:

>Translated_324_residues
MKVGFIIDNLNSFNISKDSTYMMLHAAQDKGWEIYTFYLNDLSIINGKPKGDALKIKIHKTKQDWYEILSQRHDFSLLDL
DCIFMRKDPPFNMEYIYVTYMLDLAKKNDVLVVNNPQALRDFNEKVAISNYPKFAPHTLITRSYKQINEFYEKHKDIIVK
PLDGMGGSSIFRIKEGDKNKNVILEILTQHQSRYIMVQDYQKAIKEGDKRILIVNGEPIKYLLARVPSDSDNRGNLAAGA
TAEVRELQDSDYKIAKKVAKKLKKEGVMFAGIDVIGDKLTEVNITSPTGIQEIYKATKINAASLLMQAVEKKINKMRQEH
ENGE
>Mature_324_residues
MKVGFIIDNLNSFNISKDSTYMMLHAAQDKGWEIYTFYLNDLSIINGKPKGDALKIKIHKTKQDWYEILSQRHDFSLLDL
DCIFMRKDPPFNMEYIYVTYMLDLAKKNDVLVVNNPQALRDFNEKVAISNYPKFAPHTLITRSYKQINEFYEKHKDIIVK
PLDGMGGSSIFRIKEGDKNKNVILEILTQHQSRYIMVQDYQKAIKEGDKRILIVNGEPIKYLLARVPSDSDNRGNLAAGA
TAEVRELQDSDYKIAKKVAKKLKKEGVMFAGIDVIGDKLTEVNITSPTGIQEIYKATKINAASLLMQAVEKKINKMRQEH
ENGE

Specific function: Glutathione biosynthesis; second step. [C]

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1789316, Length=315, Percent_Identity=46.984126984127, Blast_Score=335, Evalue=2e-93,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR006284
- InterPro:   IPR004218
- InterPro:   IPR004215
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF02955 GSH-S_ATP; PF02951 GSH-S_N [H]

EC number: =6.3.2.3 [H]

Molecular weight: Translated: 37160; Mature: 37160

Theoretical pI: Translated: 9.43; Mature: 9.43

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVGFIIDNLNSFNISKDSTYMMLHAAQDKGWEIYTFYLNDLSIINGKPKGDALKIKIHK
CEEEEEEECCCCCCCCCCCEEEEEEECCCCCCEEEEEEECCEEEECCCCCCCEEEEEEEC
TKQDWYEILSQRHDFSLLDLDCIFMRKDPPFNMEYIYVTYMLDLAKKNDVLVVNNPQALR
CHHHHHHHHHCCCCCEEEEEEEEEEECCCCCCEEEEEEEEEEHHCCCCCEEEEECCHHHH
DFNEKVAISNYPKFAPHTLITRSYKQINEFYEKHKDIIVKPLDGMGGSSIFRIKEGDKNK
HCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCEEEEEECCCCCC
NVILEILTQHQSRYIMVQDYQKAIKEGDKRILIVNGEPIKYLLARVPSDSDNRGNLAAGA
HHHHHHHHHCCCCEEEEHHHHHHHHCCCCEEEEECCCCHHHHHHHCCCCCCCCCCEECCC
TAEVRELQDSDYKIAKKVAKKLKKEGVMFAGIDVIGDKLTEVNITSPTGIQEIYKATKIN
CHHHHHHCCCCHHHHHHHHHHHHHCCCEEEEHHHHCCCEEEEEECCCCCHHHHHHHHCCC
AASLLMQAVEKKINKMRQEHENGE
HHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKVGFIIDNLNSFNISKDSTYMMLHAAQDKGWEIYTFYLNDLSIINGKPKGDALKIKIHK
CEEEEEEECCCCCCCCCCCEEEEEEECCCCCCEEEEEEECCEEEECCCCCCCEEEEEEEC
TKQDWYEILSQRHDFSLLDLDCIFMRKDPPFNMEYIYVTYMLDLAKKNDVLVVNNPQALR
CHHHHHHHHHCCCCCEEEEEEEEEEECCCCCCEEEEEEEEEEHHCCCCCEEEEECCHHHH
DFNEKVAISNYPKFAPHTLITRSYKQINEFYEKHKDIIVKPLDGMGGSSIFRIKEGDKNK
HCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCEEEEEECCCCCC
NVILEILTQHQSRYIMVQDYQKAIKEGDKRILIVNGEPIKYLLARVPSDSDNRGNLAAGA
HHHHHHHHHCCCCEEEEHHHHHHHHCCCCEEEEECCCCHHHHHHHCCCCCCCCCCEECCC
TAEVRELQDSDYKIAKKVAKKLKKEGVMFAGIDVIGDKLTEVNITSPTGIQEIYKATKIN
CHHHHHHCCCCHHHHHHHHHHHHHCCCEEEEHHHHCCCEEEEEECCCCCHHHHHHHHCCC
AASLLMQAVEKKINKMRQEHENGE
HHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]