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The map label for this gene is birA [H]

Identifier: 89256585

GI number: 89256585

Start: 1216412

End: 1217227

Strand: Reverse

Name: birA [H]

Synonym: FTL_1276

Alternate gene names: 89256585

Gene position: 1217227-1216412 (Counterclockwise)

Preceding gene: 89256586

Following gene: 89256580

Centisome position: 64.2

GC content: 27.33

Gene sequence:

>816_bases
ATGAATAAAACTCAGCTACAAATATTAACCTTTTTAGATGATGAAAAATATGTAATTGGTGAGGATATTGCTCAAAAATT
AGGCATATCTAGAGCAGCTATTTCTAAAAATATCAAAGCACTCAAAGACAGTTATCAAATAACAGTTTCTTCTAACTCAA
GAGTTGGATATCGACTCCAAAAAAAATTAGATCTAATAAAAGTTGATGAACTAGCTAAATTTTTCAAAAATATAAATTAC
TTTTATTCTGTAGATTCAACATCAAAATATGCTATAGAAAATCAAGCTAATTTTAGTGAGAGTGCTATTTTTATTTCAGA
ATTTCAAACAGATGGATTTGGACGATTTAAGCGTAGATGGATTTCACCATTTGGTAAAAATATTTACTGTACTATGCTTG
AAATAGTAGAGCTAGACATATCAAAACTTAATGGTTTATCCTTAGTTGTAGGAATCAGTATTGCGAGAGTTCTAAAAAAT
ATTAACTTGAATGCTAAATTAAAATGGCCAAATGATATCTATGTGGATGATAAGAAGATTGCTGGCGTTATTATAAATGT
TAGTGCTGAAATTAATGGTAGAGCTAAGCTATTTATAGGTTTTGGTATAAACGTTAATATGCAATATAACGAAGAAATAT
CTAAAGATTGGACATCAATAAAGTTACAATCTCAACGCCATGCTAATCGAACTAACCTAACTATACAAATAGTAAAAGCT
ATTAAAGAAGATTTATCAGTTTTCATTGATAAAGGTTTTACACATTTCAAAAATGAATTTGAATCACTTAATTATCTAAA
AGATAAAGTATTTTAA

Upstream 100 bases:

>100_bases
GAATGGTCAGAAACGGAGGGAGATTTTCAGGTTTTTAGCGCTGCAGCTGTTTATTTGGATAGAAGTAGCAATTTATAATT
TTTTAATAGAGTAATGTTAA

Downstream 100 bases:

>100_bases
TTTGCTCTAGCAGATAAAACTTATAATAACTGTAGCTATGTTGACTTAGCTAATAATGGCGAAATTCTTATTAAGAATAG
CGAAGGGAATTATTCATTTT

Product: bifunctional biotin operon repressor/biotin synthetase BirA

Products: NA

Alternate protein names: Biotin operon repressor; Biotin--[acetyl-CoA-carboxylase] synthetase; Biotin--protein ligase [H]

Number of amino acids: Translated: 271; Mature: 271

Protein sequence:

>271_residues
MNKTQLQILTFLDDEKYVIGEDIAQKLGISRAAISKNIKALKDSYQITVSSNSRVGYRLQKKLDLIKVDELAKFFKNINY
FYSVDSTSKYAIENQANFSESAIFISEFQTDGFGRFKRRWISPFGKNIYCTMLEIVELDISKLNGLSLVVGISIARVLKN
INLNAKLKWPNDIYVDDKKIAGVIINVSAEINGRAKLFIGFGINVNMQYNEEISKDWTSIKLQSQRHANRTNLTIQIVKA
IKEDLSVFIDKGFTHFKNEFESLNYLKDKVF

Sequences:

>Translated_271_residues
MNKTQLQILTFLDDEKYVIGEDIAQKLGISRAAISKNIKALKDSYQITVSSNSRVGYRLQKKLDLIKVDELAKFFKNINY
FYSVDSTSKYAIENQANFSESAIFISEFQTDGFGRFKRRWISPFGKNIYCTMLEIVELDISKLNGLSLVVGISIARVLKN
INLNAKLKWPNDIYVDDKKIAGVIINVSAEINGRAKLFIGFGINVNMQYNEEISKDWTSIKLQSQRHANRTNLTIQIVKA
IKEDLSVFIDKGFTHFKNEFESLNYLKDKVF
>Mature_271_residues
MNKTQLQILTFLDDEKYVIGEDIAQKLGISRAAISKNIKALKDSYQITVSSNSRVGYRLQKKLDLIKVDELAKFFKNINY
FYSVDSTSKYAIENQANFSESAIFISEFQTDGFGRFKRRWISPFGKNIYCTMLEIVELDISKLNGLSLVVGISIARVLKN
INLNAKLKWPNDIYVDDKKIAGVIINVSAEINGRAKLFIGFGINVNMQYNEEISKDWTSIKLQSQRHANRTNLTIQIVKA
IKEDLSVFIDKGFTHFKNEFESLNYLKDKVF

Specific function: BirA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl-CoA:carbon-dioxide ligase. This protein also activates biotin to form biotinyl-5'- adenylate and transfers the biotin moiety to biotin-accepting protein

COG id: COG0340

COG function: function code H; Biotin-(acetyl-CoA carboxylase) ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the biotin--protein ligase family [H]

Homologues:

Organism=Escherichia coli, GI1790408, Length=268, Percent_Identity=29.1044776119403, Blast_Score=151, Evalue=4e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004408
- InterPro:   IPR004409
- InterPro:   IPR003142
- InterPro:   IPR004143
- InterPro:   IPR013196
- InterPro:   IPR008988
- InterPro:   IPR011991 [H]

Pfam domain/function: PF02237 BPL_C; PF03099 BPL_LipA_LipB; PF08279 HTH_11 [H]

EC number: =6.3.4.15 [H]

Molecular weight: Translated: 31068; Mature: 31068

Theoretical pI: Translated: 9.90; Mature: 9.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKTQLQILTFLDDEKYVIGEDIAQKLGISRAAISKNIKALKDSYQITVSSNSRVGYRLQ
CCCCEEEEEEEECCCCEEHHHHHHHHHCCHHHHHHHHHHHHCCCEEEEEECCCCCCEEHH
KKLDLIKVDELAKFFKNINYFYSVDSTSKYAIENQANFSESAIFISEFQTDGFGRFKRRW
HHCCEEEHHHHHHHHHCCCEEEEECCCCEEEECCCCCCCCCEEEEEECCCCCHHHHHHHH
ISPFGKNIYCTMLEIVELDISKLNGLSLVVGISIARVLKNINLNAKLKWPNDIYVDDKKI
HCCCCCCEEEHHHHHHHHHHHHCCCEEEEHHHHHHHHHHCCCCCEEEECCCCEEECCCEE
AGVIINVSAEINGRAKLFIGFGINVNMQYNEEISKDWTSIKLQSQRHANRTNLTIQIVKA
EEEEEEEEEEECCCEEEEEEEEEEEEEEECCHHHCCHHEEEEEHHHCCCCCCHHHHHHHH
IKEDLSVFIDKGFTHFKNEFESLNYLKDKVF
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNKTQLQILTFLDDEKYVIGEDIAQKLGISRAAISKNIKALKDSYQITVSSNSRVGYRLQ
CCCCEEEEEEEECCCCEEHHHHHHHHHCCHHHHHHHHHHHHCCCEEEEEECCCCCCEEHH
KKLDLIKVDELAKFFKNINYFYSVDSTSKYAIENQANFSESAIFISEFQTDGFGRFKRRW
HHCCEEEHHHHHHHHHCCCEEEEECCCCEEEECCCCCCCCCEEEEEECCCCCHHHHHHHH
ISPFGKNIYCTMLEIVELDISKLNGLSLVVGISIARVLKNINLNAKLKWPNDIYVDDKKI
HCCCCCCEEEHHHHHHHHHHHHCCCEEEEHHHHHHHHHHCCCCCEEEECCCCEEECCCEE
AGVIINVSAEINGRAKLFIGFGINVNMQYNEEISKDWTSIKLQSQRHANRTNLTIQIVKA
EEEEEEEEEEECCCEEEEEEEEEEEEEEECCHHHCCHHEEEEEHHHCCCCCCHHHHHHHH
IKEDLSVFIDKGFTHFKNEFESLNYLKDKVF
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3899863; 3536662; 8265357; 9278503; 1311302; 1328157; 1409631; 11353844 [H]