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The map label for this gene is cls [H]

Identifier: 89256525

GI number: 89256525

Start: 1153893

End: 1155320

Strand: Reverse

Name: cls [H]

Synonym: FTL_1204

Alternate gene names: 89256525

Gene position: 1155320-1153893 (Counterclockwise)

Preceding gene: 89256526

Following gene: 89256524

Centisome position: 60.93

GC content: 29.83

Gene sequence:

>1428_bases
ATGGAAAAATTCTTATCAAGTCTAATATATATATTAGAAGCCAATACAATCTTATTTATTTGTCAAATTTTTACTATTTT
TATTGTATTAAAACTAATTGTAGATAAAAGATCAGCATCAAATATCCTCGCATGGATTTTGGCGATATTGTTTATCCCTT
ACTTTGCTATTCCATTTTTCTTTATCTTTCAACGTAAAGATAAACGTAAACTTTGGCAAAAAGGTGCAATGAGTATAGAG
CCAGAACCTATAGCAAATACTAGTCTAAGCGAAAGTGAACATAAGGGATTACCTATAAATGTAATAAAAACTTTTATAAA
TCTTGAACTTCCTACACTTACACAAAAAAATACTTTTGAAATATATACAAATGGTACTGATTCTTTCAAAGCTTTTATCC
AAGCTATAGAATCAGCAAAGCAAAGTATATACATTCAAACATATATAATCAAAAATGATATAACTTCAAAACTCGTAATT
AGTGCTTTAGAAAAAAAAGCTGCTGAAGGTATAGAAATTAAAATGATGATAGACTCTCTAGGCTCTTTTTATGTCTATCG
GCATAACAAAAGAATTTTTAAAAACTTACGTAAACTAGGTGCGAAAGTAGTATTTTTTATGCCAGTAATTTCTAATCCTA
TGCGTAACTATATCAATTATAGGAATCATCGTAAAATTTTTATTTTTGATAACAAAATAGCTTTTAGTGGCGGTATGAAT
ATTGGTGACGAGTATATGTCACCTACAGCTCATGACGGTATGTGGGGTGATTTACTCTTTAAAATTCAGGGGCAATCTTT
AGTTTATTTCCTAAAAATTTTCTGTTCAGATTGGCATTTTGCAACATCTGAGGAGTTAAACCCGAAAATTGAGAACACCA
TACAAGAAGAATGTTTTACACAAGTAGTTCCCTCAGGTCCAGATATGCAAAAAAACCAGCTCTATTCGGGTTTGATTACA
GCGATTAATTCTGCGCAAAAAAGACTATGGATTATCACACCCTATTTAATTCCATCGCTTGATTTACTTCAAGCTATACT
CCTTGCTAAATGTAGAGGTATTGATGTCAAAGTGATTACTCCTAAAAATTCAGATCATAAAATAATTAATCGTGCTAGAT
CTAGTTATATTAGAGATTTACTTGAATATAATATAGATGTTTATTTTACAAAAAATATGTTGCATGCAAAAGCTGTACTT
GTAGATTCTAATATCGCGATACTTGGCTCAGTAAATCTAGATAATCGTAGTTTATTTTTAAATTATGAAGTTGCTACTTT
TATATATACTCGCAAACAAGTAGATAAATTATATCAATGGGCAGAAAACATAATTAAAGACTCTGATAGAGATGCATCAC
ATTTGCCAACCAAACGCAGCAGTTTAATTGTTGAAAGCATTATGAAAATACTTACACCTCTAATGTAA

Upstream 100 bases:

>100_bases
TTATCTTAGTCTTTACACGTAATACTTCACAAAACATCTCTATCTAACACACTAGTAACTGTGATAAAATTTTTTAAAAG
GTCATTCTATTTTAATAGTA

Downstream 100 bases:

>100_bases
TAACTTAATTTAAGGCAAAATTTTATATAACTACCGAGAATATTATCTGTGTGTTACAATTTAGCATAATATAAGCTTAA
CGCTATTATAAATGGAAAAT

Product: cardiolipin synthetase

Products: Biphosphatidylglycerol; Glycerol. [C]

Alternate protein names: CL synthase [H]

Number of amino acids: Translated: 475; Mature: 475

Protein sequence:

>475_residues
MEKFLSSLIYILEANTILFICQIFTIFIVLKLIVDKRSASNILAWILAILFIPYFAIPFFFIFQRKDKRKLWQKGAMSIE
PEPIANTSLSESEHKGLPINVIKTFINLELPTLTQKNTFEIYTNGTDSFKAFIQAIESAKQSIYIQTYIIKNDITSKLVI
SALEKKAAEGIEIKMMIDSLGSFYVYRHNKRIFKNLRKLGAKVVFFMPVISNPMRNYINYRNHRKIFIFDNKIAFSGGMN
IGDEYMSPTAHDGMWGDLLFKIQGQSLVYFLKIFCSDWHFATSEELNPKIENTIQEECFTQVVPSGPDMQKNQLYSGLIT
AINSAQKRLWIITPYLIPSLDLLQAILLAKCRGIDVKVITPKNSDHKIINRARSSYIRDLLEYNIDVYFTKNMLHAKAVL
VDSNIAILGSVNLDNRSLFLNYEVATFIYTRKQVDKLYQWAENIIKDSDRDASHLPTKRSSLIVESIMKILTPLM

Sequences:

>Translated_475_residues
MEKFLSSLIYILEANTILFICQIFTIFIVLKLIVDKRSASNILAWILAILFIPYFAIPFFFIFQRKDKRKLWQKGAMSIE
PEPIANTSLSESEHKGLPINVIKTFINLELPTLTQKNTFEIYTNGTDSFKAFIQAIESAKQSIYIQTYIIKNDITSKLVI
SALEKKAAEGIEIKMMIDSLGSFYVYRHNKRIFKNLRKLGAKVVFFMPVISNPMRNYINYRNHRKIFIFDNKIAFSGGMN
IGDEYMSPTAHDGMWGDLLFKIQGQSLVYFLKIFCSDWHFATSEELNPKIENTIQEECFTQVVPSGPDMQKNQLYSGLIT
AINSAQKRLWIITPYLIPSLDLLQAILLAKCRGIDVKVITPKNSDHKIINRARSSYIRDLLEYNIDVYFTKNMLHAKAVL
VDSNIAILGSVNLDNRSLFLNYEVATFIYTRKQVDKLYQWAENIIKDSDRDASHLPTKRSSLIVESIMKILTPLM
>Mature_475_residues
MEKFLSSLIYILEANTILFICQIFTIFIVLKLIVDKRSASNILAWILAILFIPYFAIPFFFIFQRKDKRKLWQKGAMSIE
PEPIANTSLSESEHKGLPINVIKTFINLELPTLTQKNTFEIYTNGTDSFKAFIQAIESAKQSIYIQTYIIKNDITSKLVI
SALEKKAAEGIEIKMMIDSLGSFYVYRHNKRIFKNLRKLGAKVVFFMPVISNPMRNYINYRNHRKIFIFDNKIAFSGGMN
IGDEYMSPTAHDGMWGDLLFKIQGQSLVYFLKIFCSDWHFATSEELNPKIENTIQEECFTQVVPSGPDMQKNQLYSGLIT
AINSAQKRLWIITPYLIPSLDLLQAILLAKCRGIDVKVITPKNSDHKIINRARSSYIRDLLEYNIDVYFTKNMLHAKAVL
VDSNIAILGSVNLDNRSLFLNYEVATFIYTRKQVDKLYQWAENIIKDSDRDASHLPTKRSSLIVESIMKILTPLM

Specific function: Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol [H]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787502, Length=420, Percent_Identity=29.0476190476191, Blast_Score=183, Evalue=2e-47,
Organism=Escherichia coli, GI1787007, Length=335, Percent_Identity=25.3731343283582, Blast_Score=91, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022924
- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.- [C]

Molecular weight: Translated: 54721; Mature: 54721

Theoretical pI: Translated: 9.70; Mature: 9.70

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKFLSSLIYILEANTILFICQIFTIFIVLKLIVDKRSASNILAWILAILFIPYFAIPFF
CHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
FIFQRKDKRKLWQKGAMSIEPEPIANTSLSESEHKGLPINVIKTFINLELPTLTQKNTFE
HHHHCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCEE
IYTNGTDSFKAFIQAIESAKQSIYIQTYIIKNDITSKLVISALEKKAAEGIEIKMMIDSL
EEECCCHHHHHHHHHHHHHHHEEEEEEEEEECCCHHHHHHHHHHHHHHCCCEEEEEHHCC
GSFYVYRHNKRIFKNLRKLGAKVVFFMPVISNPMRNYINYRNHRKIFIFDNKIAFSGGMN
CCEEEEECCHHHHHHHHHHCCCEEEEEHHHCCHHHHHHCCCCCCEEEEEECEEEECCCCC
IGDEYMSPTAHDGMWGDLLFKIQGQSLVYFLKIFCSDWHFATSEELNPKIENTIQEECFT
CCHHHCCCCCCCCCCEEEEEEECCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHH
QVVPSGPDMQKNQLYSGLITAINSAQKRLWIITPYLIPSLDLLQAILLAKCRGIDVKVIT
HHCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECHHHCCCHHHHHHHHHHHHCCCEEEEEC
PKNSDHKIINRARSSYIRDLLEYNIDVYFTKNMLHAKAVLVDSNIAILGSVNLDNRSLFL
CCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCHHHEEEEEEECCEEEEEEECCCCCEEEE
NYEVATFIYTRKQVDKLYQWAENIIKDSDRDASHLPTKRSSLIVESIMKILTPLM
EEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MEKFLSSLIYILEANTILFICQIFTIFIVLKLIVDKRSASNILAWILAILFIPYFAIPFF
CHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
FIFQRKDKRKLWQKGAMSIEPEPIANTSLSESEHKGLPINVIKTFINLELPTLTQKNTFE
HHHHCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCEE
IYTNGTDSFKAFIQAIESAKQSIYIQTYIIKNDITSKLVISALEKKAAEGIEIKMMIDSL
EEECCCHHHHHHHHHHHHHHHEEEEEEEEEECCCHHHHHHHHHHHHHHCCCEEEEEHHCC
GSFYVYRHNKRIFKNLRKLGAKVVFFMPVISNPMRNYINYRNHRKIFIFDNKIAFSGGMN
CCEEEEECCHHHHHHHHHHCCCEEEEEHHHCCHHHHHHCCCCCCEEEEEECEEEECCCCC
IGDEYMSPTAHDGMWGDLLFKIQGQSLVYFLKIFCSDWHFATSEELNPKIENTIQEECFT
CCHHHCCCCCCCCCCEEEEEEECCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHH
QVVPSGPDMQKNQLYSGLITAINSAQKRLWIITPYLIPSLDLLQAILLAKCRGIDVKVIT
HHCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECHHHCCCHHHHHHHHHHHHCCCEEEEEC
PKNSDHKIINRARSSYIRDLLEYNIDVYFTKNMLHAKAVLVDSNIAILGSVNLDNRSLFL
CCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCHHHEEEEEEECCEEEEEEECCCCCEEEE
NYEVATFIYTRKQVDKLYQWAENIIKDSDRDASHLPTKRSSLIVESIMKILTPLM
EEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Phosphatidylglycerol [C]

Specific reaction: 2 Phosphatidylglycerol = Biphosphatidylglycerol + Glycerol. [C]

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]