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The map label for this gene is grpE [H]

Identifier: 89256511

GI number: 89256511

Start: 1138367

End: 1138954

Strand: Direct

Name: grpE [H]

Synonym: FTL_1190

Alternate gene names: 89256511

Gene position: 1138367-1138954 (Clockwise)

Preceding gene: 89256504

Following gene: 89256512

Centisome position: 60.04

GC content: 33.16

Gene sequence:

>588_bases
ATGAGTAAGCAAGAAAAAAGTAATGTAGAAGATAAAAGTCTTGATATTGAGACAGCAGCACAAGTAGAAACAGCACAAGA
ATCTGCTAGTGGAGCTTTAGAAGAATTATCTGTAGAAGAACAGCTAGAAAGAGCAAAAGATACTATAAAAGAACTTGAAG
ATAGTTGTGATCAATTTAAAGATGAGGCTTTAAGAGCGAAGGCTGAAATGGAAAACATTCGTAAAAGAGCTGAAAGAGAT
GTATCAAATGCACGCAAATTTGGTATAGAGAAGTTTGCTAAAGAGCTTTTGCCAGTAATTGATAGTATTGAGCAAGCATT
AAAGCATGAGGTTAAGCTTGAAGAAGCTATCGCAATGAAAGAAGGTATTGAGTTAACAGCAAAAATGCTGGTTGATATAC
TTAAGAAAAATGGTGTAGAAGAGTTAGATCCAAAAGGAGAGAAATTTGACCCTAATCTACATGAAGCTATGGCAATGATT
CCTAACCCTGAATTTGAAGATAATACTATTTTTGATGTTTTTCAAAAGGGTTATATGTTAAATGGTCGTATTGTTAGAGC
TGCAAAAGTTGTTATAGTAAAAAATTAA

Upstream 100 bases:

>100_bases
AAAAAATACCAATAATTTCTTGAAAATACAGAATTAATCACTATCTATAAGACATCTAACAAATTGTAGTATTAACAGAA
CTAAAATAAAAGGTTGTTTT

Downstream 100 bases:

>100_bases
AAAAAATACTTGAAAAGATTATAAATATGCCCATCTAGTAAGTAGTCAGATGGATTTAAAATTTTTGGAAAAAATTACAG
AATAAAAACAGGAGAATCAA

Product: heat shock protein GrpE

Products: NA

Alternate protein names: HSP-70 cofactor [H]

Number of amino acids: Translated: 195; Mature: 194

Protein sequence:

>195_residues
MSKQEKSNVEDKSLDIETAAQVETAQESASGALEELSVEEQLERAKDTIKELEDSCDQFKDEALRAKAEMENIRKRAERD
VSNARKFGIEKFAKELLPVIDSIEQALKHEVKLEEAIAMKEGIELTAKMLVDILKKNGVEELDPKGEKFDPNLHEAMAMI
PNPEFEDNTIFDVFQKGYMLNGRIVRAAKVVIVKN

Sequences:

>Translated_195_residues
MSKQEKSNVEDKSLDIETAAQVETAQESASGALEELSVEEQLERAKDTIKELEDSCDQFKDEALRAKAEMENIRKRAERD
VSNARKFGIEKFAKELLPVIDSIEQALKHEVKLEEAIAMKEGIELTAKMLVDILKKNGVEELDPKGEKFDPNLHEAMAMI
PNPEFEDNTIFDVFQKGYMLNGRIVRAAKVVIVKN
>Mature_194_residues
SKQEKSNVEDKSLDIETAAQVETAQESASGALEELSVEEQLERAKDTIKELEDSCDQFKDEALRAKAEMENIRKRAERDV
SNARKFGIEKFAKELLPVIDSIEQALKHEVKLEEAIAMKEGIELTAKMLVDILKKNGVEELDPKGEKFDPNLHEAMAMIP
NPEFEDNTIFDVFQKGYMLNGRIVRAAKVVIVKN

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family [H]

Homologues:

Organism=Homo sapiens, GI24308295, Length=150, Percent_Identity=35.3333333333333, Blast_Score=84, Evalue=6e-17,
Organism=Homo sapiens, GI40255109, Length=152, Percent_Identity=33.5526315789474, Blast_Score=83, Evalue=2e-16,
Organism=Escherichia coli, GI1788967, Length=136, Percent_Identity=47.0588235294118, Blast_Score=126, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17552458, Length=144, Percent_Identity=38.1944444444444, Blast_Score=84, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6324806, Length=144, Percent_Identity=40.2777777777778, Blast_Score=110, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24653432, Length=139, Percent_Identity=37.410071942446, Blast_Score=92, Evalue=3e-19,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012 [H]

Pfam domain/function: PF01025 GrpE [H]

EC number: NA

Molecular weight: Translated: 22036; Mature: 21905

Theoretical pI: Translated: 4.51; Mature: 4.51

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKQEKSNVEDKSLDIETAAQVETAQESASGALEELSVEEQLERAKDTIKELEDSCDQFK
CCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DEALRAKAEMENIRKRAERDVSNARKFGIEKFAKELLPVIDSIEQALKHEVKLEEAIAMK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EGIELTAKMLVDILKKNGVEELDPKGEKFDPNLHEAMAMIPNPEFEDNTIFDVFQKGYML
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHCCCCC
NGRIVRAAKVVIVKN
CCEEEEHHEEEEECC
>Mature Secondary Structure 
SKQEKSNVEDKSLDIETAAQVETAQESASGALEELSVEEQLERAKDTIKELEDSCDQFK
CCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DEALRAKAEMENIRKRAERDVSNARKFGIEKFAKELLPVIDSIEQALKHEVKLEEAIAMK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EGIELTAKMLVDILKKNGVEELDPKGEKFDPNLHEAMAMIPNPEFEDNTIFDVFQKGYML
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHCCCCC
NGRIVRAAKVVIVKN
CCEEEEHHEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA