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The map label for this gene is pykA [H]

Identifier: 89256472

GI number: 89256472

Start: 1090403

End: 1091839

Strand: Direct

Name: pykA [H]

Synonym: FTL_1148

Alternate gene names: 89256472

Gene position: 1090403-1091839 (Clockwise)

Preceding gene: 89256471

Following gene: 89256473

Centisome position: 57.51

GC content: 37.16

Gene sequence:

>1437_bases
ATGAGAAGAACAAAAATTTTAGCTACTCTTGGTCCTGCTAGTGAATCAAGAGAAGCTCTAACAGAGATGATTAAAGCAGG
GGTTAATGCAGTTAGATGTAATTTTTCGCATGGCTCTGCTGAAGATCATCGCAAAAGAGTTGATTTAATTCGTCAAATCG
CAAAAGAGCAAGATACACATGTTGGTATTTTAGCTGATTTACAGGGACCAAAAATCAGATTATCAAAATTTAAAAATGGT
TCCGTAGAAATTAAGAAAGGTCAAAAATTTACACTGGATGCTGATCTTGGTATTAATGATGGTGATGAGAATACTGTAGG
GATTGACTATAAAGAACTCATTCAAGATGTAAAAAAAGGCGATATACTACTTGTAGATGATGGCAAAATTGTCTTAGAGG
TTGATTCTGTAAAAGGAAATAAGGCAGTCACTAAAGTTGTCGTTGGCGGTAAAGTTTCTAATAATAAAGGCATCAATAAG
AAAGGTGGCGGACTTACAGCACCTGCACTTACTGAGAAAGACAAAGAAGATATAAAAATAGCTGCGATGCTACAAGTGGA
TTTCTTAGCGGTATCATTTGTCAGAGATGGTAAGGATATGGAGTATGCACGTCAACTTGTCCACGAAGCCGGTTGGAGAC
CTGCTATGGTGGCTAAAATTGAGCGTGCAGAGGCTGTCTTGGAAGATAACCTTAAGAGTATTGTTGATGCTTCTGACCTT
GTAATGGTAGCGCGTGGTGATTTGGCTGTTGAAATTGGTGATGAGAATGTGCCAACTATACAGAAGCTTATTATCAATAC
AGCTAGACGAAATGAAAAAGGTTCTATTACTGCTACACAAATGATGGAGTCGATGATAGAGAACTCTTCACCAACTCGTG
CTGAAGCATCTGATGTTGCTAATGCTGTTTTTGATGGCACAGATGCTGTAATGTTATCAGCTGAAACAGCGGTAGGTAAA
TATCCTGTTGAAACAGTTTCTGCAATGTCAAGGATATGTAAATCTGCTGAAAAAAGTAAGTATACACATATTTCTAAAAA
GCAAAAAATAGCTGATTGTGATAGGATTGATCATGCTGTATCTGTTGCAGCAGTCAAAATAGCTAACGATATTGGAGCTA
AAGGATTAATTGTATTGACAGAGGGTGGTAATACTTCGCGTTGGATGTCACGAATAAATACTGATTTACCTATTTACGCA
TTATCAAGAAACGCAACAACTTTAGGTGCGATGACATTATTTAGAGGTGTGGTACCAATATACTTTGATTCAACTAGAAT
GTCTAAGTTGTATGTAAATAGATCAGCTTGTATGGAGCTAGAGAGCAGAAATTTAGCCAAAGATGGTGATATTTTTGTGC
TTACTAGTGGCGATAGTATGGGTGTACATGGAAGCACTAATAAGATTAAAGTGATAATTGCAGGTCAGGTAAGATAA

Upstream 100 bases:

>100_bases
AACAGCTGGTGGAGCATTCTTAGAGTTTCTTGAAGGCAAGAAATTACCAGCTATAGAGATACTTAAAGAAAAAGCAATCA
GGTAATTTAGGATAATTTAT

Downstream 100 bases:

>100_bases
AAGGAGATAATAATAATAATGGCTTTAGTTTCATTGCGTCAACTATTAGATCATGCTGCTGAGCATGGTTATGGATTACC
AGCTTTTAATGTTAATAACC

Product: pyruvate kinase

Products: NA

Alternate protein names: PK-2 [H]

Number of amino acids: Translated: 478; Mature: 478

Protein sequence:

>478_residues
MRRTKILATLGPASESREALTEMIKAGVNAVRCNFSHGSAEDHRKRVDLIRQIAKEQDTHVGILADLQGPKIRLSKFKNG
SVEIKKGQKFTLDADLGINDGDENTVGIDYKELIQDVKKGDILLVDDGKIVLEVDSVKGNKAVTKVVVGGKVSNNKGINK
KGGGLTAPALTEKDKEDIKIAAMLQVDFLAVSFVRDGKDMEYARQLVHEAGWRPAMVAKIERAEAVLEDNLKSIVDASDL
VMVARGDLAVEIGDENVPTIQKLIINTARRNEKGSITATQMMESMIENSSPTRAEASDVANAVFDGTDAVMLSAETAVGK
YPVETVSAMSRICKSAEKSKYTHISKKQKIADCDRIDHAVSVAAVKIANDIGAKGLIVLTEGGNTSRWMSRINTDLPIYA
LSRNATTLGAMTLFRGVVPIYFDSTRMSKLYVNRSACMELESRNLAKDGDIFVLTSGDSMGVHGSTNKIKVIIAGQVR

Sequences:

>Translated_478_residues
MRRTKILATLGPASESREALTEMIKAGVNAVRCNFSHGSAEDHRKRVDLIRQIAKEQDTHVGILADLQGPKIRLSKFKNG
SVEIKKGQKFTLDADLGINDGDENTVGIDYKELIQDVKKGDILLVDDGKIVLEVDSVKGNKAVTKVVVGGKVSNNKGINK
KGGGLTAPALTEKDKEDIKIAAMLQVDFLAVSFVRDGKDMEYARQLVHEAGWRPAMVAKIERAEAVLEDNLKSIVDASDL
VMVARGDLAVEIGDENVPTIQKLIINTARRNEKGSITATQMMESMIENSSPTRAEASDVANAVFDGTDAVMLSAETAVGK
YPVETVSAMSRICKSAEKSKYTHISKKQKIADCDRIDHAVSVAAVKIANDIGAKGLIVLTEGGNTSRWMSRINTDLPIYA
LSRNATTLGAMTLFRGVVPIYFDSTRMSKLYVNRSACMELESRNLAKDGDIFVLTSGDSMGVHGSTNKIKVIIAGQVR
>Mature_478_residues
MRRTKILATLGPASESREALTEMIKAGVNAVRCNFSHGSAEDHRKRVDLIRQIAKEQDTHVGILADLQGPKIRLSKFKNG
SVEIKKGQKFTLDADLGINDGDENTVGIDYKELIQDVKKGDILLVDDGKIVLEVDSVKGNKAVTKVVVGGKVSNNKGINK
KGGGLTAPALTEKDKEDIKIAAMLQVDFLAVSFVRDGKDMEYARQLVHEAGWRPAMVAKIERAEAVLEDNLKSIVDASDL
VMVARGDLAVEIGDENVPTIQKLIINTARRNEKGSITATQMMESMIENSSPTRAEASDVANAVFDGTDAVMLSAETAVGK
YPVETVSAMSRICKSAEKSKYTHISKKQKIADCDRIDHAVSVAAVKIANDIGAKGLIVLTEGGNTSRWMSRINTDLPIYA
LSRNATTLGAMTLFRGVVPIYFDSTRMSKLYVNRSACMELESRNLAKDGDIFVLTSGDSMGVHGSTNKIKVIIAGQVR

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286418, Length=492, Percent_Identity=36.9918699186992, Blast_Score=274, Evalue=2e-73,
Organism=Homo sapiens, GI33286422, Length=491, Percent_Identity=37.0672097759674, Blast_Score=273, Evalue=3e-73,
Organism=Homo sapiens, GI33286420, Length=491, Percent_Identity=37.0672097759674, Blast_Score=273, Evalue=3e-73,
Organism=Homo sapiens, GI32967597, Length=489, Percent_Identity=35.7873210633947, Blast_Score=259, Evalue=5e-69,
Organism=Homo sapiens, GI10835121, Length=489, Percent_Identity=35.7873210633947, Blast_Score=258, Evalue=6e-69,
Organism=Homo sapiens, GI310128732, Length=289, Percent_Identity=38.0622837370242, Blast_Score=169, Evalue=4e-42,
Organism=Homo sapiens, GI310128730, Length=289, Percent_Identity=38.0622837370242, Blast_Score=169, Evalue=4e-42,
Organism=Homo sapiens, GI310128736, Length=236, Percent_Identity=36.864406779661, Blast_Score=138, Evalue=1e-32,
Organism=Homo sapiens, GI310128734, Length=236, Percent_Identity=36.864406779661, Blast_Score=138, Evalue=1e-32,
Organism=Homo sapiens, GI310128738, Length=215, Percent_Identity=34.8837209302326, Blast_Score=114, Evalue=2e-25,
Organism=Escherichia coli, GI1788160, Length=480, Percent_Identity=53.125, Blast_Score=479, Evalue=1e-136,
Organism=Escherichia coli, GI1787965, Length=476, Percent_Identity=39.9159663865546, Blast_Score=315, Evalue=3e-87,
Organism=Caenorhabditis elegans, GI17544584, Length=485, Percent_Identity=35.2577319587629, Blast_Score=278, Evalue=4e-75,
Organism=Caenorhabditis elegans, GI71984406, Length=491, Percent_Identity=35.6415478615071, Blast_Score=253, Evalue=2e-67,
Organism=Caenorhabditis elegans, GI71984413, Length=491, Percent_Identity=35.6415478615071, Blast_Score=253, Evalue=2e-67,
Organism=Caenorhabditis elegans, GI17506829, Length=491, Percent_Identity=35.6415478615071, Blast_Score=253, Evalue=2e-67,
Organism=Caenorhabditis elegans, GI17506831, Length=491, Percent_Identity=35.6415478615071, Blast_Score=253, Evalue=2e-67,
Organism=Saccharomyces cerevisiae, GI6319279, Length=488, Percent_Identity=33.8114754098361, Blast_Score=238, Evalue=1e-63,
Organism=Saccharomyces cerevisiae, GI6324923, Length=492, Percent_Identity=32.520325203252, Blast_Score=229, Evalue=1e-60,
Organism=Drosophila melanogaster, GI24648964, Length=489, Percent_Identity=34.9693251533742, Blast_Score=257, Evalue=1e-68,
Organism=Drosophila melanogaster, GI28571814, Length=489, Percent_Identity=34.9693251533742, Blast_Score=257, Evalue=1e-68,
Organism=Drosophila melanogaster, GI24648966, Length=409, Percent_Identity=38.6308068459658, Blast_Score=256, Evalue=2e-68,
Organism=Drosophila melanogaster, GI24581235, Length=488, Percent_Identity=32.172131147541, Blast_Score=227, Evalue=1e-59,
Organism=Drosophila melanogaster, GI24646914, Length=273, Percent_Identity=38.0952380952381, Blast_Score=157, Evalue=2e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 51777; Mature: 51777

Theoretical pI: Translated: 8.80; Mature: 8.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRTKILATLGPASESREALTEMIKAGVNAVRCNFSHGSAEDHRKRVDLIRQIAKEQDTH
CCCEEEEEEECCCCHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCC
VGILADLQGPKIRLSKFKNGSVEIKKGQKFTLDADLGINDGDENTVGIDYKELIQDVKKG
EEEEEECCCCEEEEEECCCCCEEEECCCEEEEEECCCCCCCCCCEECCCHHHHHHHHCCC
DILLVDDGKIVLEVDSVKGNKAVTKVVVGGKVSNNKGINKKGGGLTAPALTEKDKEDIKI
CEEEEECCEEEEEEECCCCCCEEEEEEECCEECCCCCCCCCCCCCCCCCCCCCCCCCEEE
AAMLQVDFLAVSFVRDGKDMEYARQLVHEAGWRPAMVAKIERAEAVLEDNLKSIVDASDL
EEEEEHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCE
VMVARGDLAVEIGDENVPTIQKLIINTARRNEKGSITATQMMESMIENSSPTRAEASDVA
EEEECCCEEEEECCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHH
NAVFDGTDAVMLSAETAVGKYPVETVSAMSRICKSAEKSKYTHISKKQKIADCDRIDHAV
HHHHCCCCEEEEEECHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SVAAVKIANDIGAKGLIVLTEGGNTSRWMSRINTDLPIYALSRNATTLGAMTLFRGVVPI
HHHHEEEHHCCCCCEEEEEECCCCHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHCCEEE
YFDSTRMSKLYVNRSACMELESRNLAKDGDIFVLTSGDSMGVHGSTNKIKVIIAGQVR
EECCCCHHHEEECHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCEEEEEEECCCC
>Mature Secondary Structure
MRRTKILATLGPASESREALTEMIKAGVNAVRCNFSHGSAEDHRKRVDLIRQIAKEQDTH
CCCEEEEEEECCCCHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCC
VGILADLQGPKIRLSKFKNGSVEIKKGQKFTLDADLGINDGDENTVGIDYKELIQDVKKG
EEEEEECCCCEEEEEECCCCCEEEECCCEEEEEECCCCCCCCCCEECCCHHHHHHHHCCC
DILLVDDGKIVLEVDSVKGNKAVTKVVVGGKVSNNKGINKKGGGLTAPALTEKDKEDIKI
CEEEEECCEEEEEEECCCCCCEEEEEEECCEECCCCCCCCCCCCCCCCCCCCCCCCCEEE
AAMLQVDFLAVSFVRDGKDMEYARQLVHEAGWRPAMVAKIERAEAVLEDNLKSIVDASDL
EEEEEHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCE
VMVARGDLAVEIGDENVPTIQKLIINTARRNEKGSITATQMMESMIENSSPTRAEASDVA
EEEECCCEEEEECCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHH
NAVFDGTDAVMLSAETAVGKYPVETVSAMSRICKSAEKSKYTHISKKQKIADCDRIDHAV
HHHHCCCCEEEEEECHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SVAAVKIANDIGAKGLIVLTEGGNTSRWMSRINTDLPIYALSRNATTLGAMTLFRGVVPI
HHHHEEEHHCCCCCEEEEEECCCCHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHCCEEE
YFDSTRMSKLYVNRSACMELESRNLAKDGDIFVLTSGDSMGVHGSTNKIKVIIAGQVR
EECCCCHHHEEECHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503; 1732206; 1859631; 8439398 [H]