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The map label for this gene is dacD [H]

Identifier: 89256395

GI number: 89256395

Start: 1013616

End: 1014950

Strand: Reverse

Name: dacD [H]

Synonym: FTL_1060

Alternate gene names: 89256395

Gene position: 1014950-1013616 (Counterclockwise)

Preceding gene: 89256410

Following gene: 89256394

Centisome position: 53.53

GC content: 35.96

Gene sequence:

>1335_bases
ATGAAATTGACAAAAATAGCTTTATTAATAGCTAGTATAACAGCGGGGGTAGCTTTTGCTGCGCCTAATATATCTGCCGG
CTCTGATCCTTATTTCAATGGAGCTAATGGACTCGCGCAGAAAAATATTATTATAAGACCTGCAAACATTGAGCTAGATG
CTCCAGCTTGGGTAACGATGGATTATCGTACTGGAGATATTGTTAGTGAAAAAAATATGGACGTCAGAAGAGCCCCTGCA
AGCTTAACTAAAATCATGACTTCCTATATAGTTGCTAGTGAAATAAAAGCTGGTAATCTAAGCTGGGATACAATGATCCC
AATTAGTGAGAATGCTGCTTCTACAGGTGGTTCAAAGATGTATGTTAAAGCCGGTGCTAAGGTATCTGTAAGAAATCTTG
TAACTGGTATGGATGTAGTGTCTGGTAATGATGCTACTATTGCTCTTGCTGAATATATCGGCGGAACTACACAAGCCTTT
ACTGATTTAATGAATCAAACAGCAAAGGCTATTGGCATGAATAATACTCATTTTGCTAATCCAGATGGACTTCCAGGTGG
TGAGCAATATACTACTGCTCATGATATGGCGTTGCTTGCAAGATCATACATATATAATTTCCCAGAAGCATATAAAGTAT
ACGACGATAAGGGCTTAGTTTGGAATGCCACAAAACAAGACTCGGTAAGTATTGCTGATCGTAAACAGTGCTTACCTAAG
TTTGATCGCGCTACTGGAAATGTCATAGAGAGCTATACGGTCAAAGATCTTGATGATCAAGCTAAAGATAAATGTAATAA
GCTTTTTCCTAAAGGTGATAATTTCGTATTACAAAATAATAGAAATAGATTACTATTTACATTTGATGGGGCTGATGGTA
TGAAGACTGGTCATACTGATGCTGCTGGATACTGTCTAGTTTCATCTGTTAAACAAGATGGCGAGAGATTTATTTCTGTA
GTTCTTGGTACTACTAGTTCTGCTAAGAGAGATTCAGAATCAGCTAAATTACTAAGATATGCTCTGAGTAAGTATGAAAA
TGTACTTTTATACAAAGCAAATTCTCCAGTTACAATTAGTGCTGATAATATCCCTAATGCAAAAGCTGGACAAAAGTTAA
CTGTAGCTTCAAATCAAAATATTTATAAAACAGTACCTAAAACTTATGTCCCTTATCTAAAGCAAGGTATAGAATTTAAT
CCAAATTTGAACGCTCCTATTAAAACAGGTCAAAATGTAGGTAATTTAGTGATTACACTAGGTGATACTAAGGAGGAAAT
TGCTAGTATACCTGTAGTTGCTATGAATAATGTTTCACAAAAAGGTTGGTGGTAA

Upstream 100 bases:

>100_bases
CTATCACAAAAACCTTTGTATTTATAACACATAAATATTAAAATACTTAAAGTTATGAGCTTAAAATATACACTTTGATT
AAAATTTAAAGAGGGAAAAT

Downstream 100 bases:

>100_bases
TAACCACAGAATTTGTAATTAAGGATTTATAAATGTCTGAAAGTAATAATCACAATCAACAAGAAACTTTTTTTGAGTTT
CCTTGCCAATTTCCAATAAA

Product: D-alanyl-D-alanine carboxypeptidase (penicillin binding protein) family protein

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 6b; PBP-6b [H]

Number of amino acids: Translated: 444; Mature: 444

Protein sequence:

>444_residues
MKLTKIALLIASITAGVAFAAPNISAGSDPYFNGANGLAQKNIIIRPANIELDAPAWVTMDYRTGDIVSEKNMDVRRAPA
SLTKIMTSYIVASEIKAGNLSWDTMIPISENAASTGGSKMYVKAGAKVSVRNLVTGMDVVSGNDATIALAEYIGGTTQAF
TDLMNQTAKAIGMNNTHFANPDGLPGGEQYTTAHDMALLARSYIYNFPEAYKVYDDKGLVWNATKQDSVSIADRKQCLPK
FDRATGNVIESYTVKDLDDQAKDKCNKLFPKGDNFVLQNNRNRLLFTFDGADGMKTGHTDAAGYCLVSSVKQDGERFISV
VLGTTSSAKRDSESAKLLRYALSKYENVLLYKANSPVTISADNIPNAKAGQKLTVASNQNIYKTVPKTYVPYLKQGIEFN
PNLNAPIKTGQNVGNLVITLGDTKEEIASIPVVAMNNVSQKGWW

Sequences:

>Translated_444_residues
MKLTKIALLIASITAGVAFAAPNISAGSDPYFNGANGLAQKNIIIRPANIELDAPAWVTMDYRTGDIVSEKNMDVRRAPA
SLTKIMTSYIVASEIKAGNLSWDTMIPISENAASTGGSKMYVKAGAKVSVRNLVTGMDVVSGNDATIALAEYIGGTTQAF
TDLMNQTAKAIGMNNTHFANPDGLPGGEQYTTAHDMALLARSYIYNFPEAYKVYDDKGLVWNATKQDSVSIADRKQCLPK
FDRATGNVIESYTVKDLDDQAKDKCNKLFPKGDNFVLQNNRNRLLFTFDGADGMKTGHTDAAGYCLVSSVKQDGERFISV
VLGTTSSAKRDSESAKLLRYALSKYENVLLYKANSPVTISADNIPNAKAGQKLTVASNQNIYKTVPKTYVPYLKQGIEFN
PNLNAPIKTGQNVGNLVITLGDTKEEIASIPVVAMNNVSQKGWW
>Mature_444_residues
MKLTKIALLIASITAGVAFAAPNISAGSDPYFNGANGLAQKNIIIRPANIELDAPAWVTMDYRTGDIVSEKNMDVRRAPA
SLTKIMTSYIVASEIKAGNLSWDTMIPISENAASTGGSKMYVKAGAKVSVRNLVTGMDVVSGNDATIALAEYIGGTTQAF
TDLMNQTAKAIGMNNTHFANPDGLPGGEQYTTAHDMALLARSYIYNFPEAYKVYDDKGLVWNATKQDSVSIADRKQCLPK
FDRATGNVIESYTVKDLDDQAKDKCNKLFPKGDNFVLQNNRNRLLFTFDGADGMKTGHTDAAGYCLVSSVKQDGERFISV
VLGTTSSAKRDSESAKLLRYALSKYENVLLYKANSPVTISADNIPNAKAGQKLTVASNQNIYKTVPKTYVPYLKQGIEFN
PNLNAPIKTGQNVGNLVITLGDTKEEIASIPVVAMNNVSQKGWW

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential). Note=N-terminal lies in the periplasmic space (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI87082022, Length=427, Percent_Identity=31.3817330210773, Blast_Score=209, Evalue=3e-55,
Organism=Escherichia coli, GI1787062, Length=455, Percent_Identity=31.8681318681319, Blast_Score=202, Evalue=3e-53,
Organism=Escherichia coli, GI1786851, Length=408, Percent_Identity=30.8823529411765, Blast_Score=193, Evalue=2e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 48034; Mature: 48034

Theoretical pI: Translated: 9.03; Mature: 9.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLTKIALLIASITAGVAFAAPNISAGSDPYFNGANGLAQKNIIIRPANIELDAPAWVTM
CCHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCCCCCCCCCCEEEECCCEEECCCEEEEE
DYRTGDIVSEKNMDVRRAPASLTKIMTSYIVASEIKAGNLSWDTMIPISENAASTGGSKM
ECCCCCEECCCCCCHHHCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCEE
YVKAGAKVSVRNLVTGMDVVSGNDATIALAEYIGGTTQAFTDLMNQTAKAIGMNNTHFAN
EEECCCEEEHHHHHHCCEEECCCCCEEEEHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCC
PDGLPGGEQYTTAHDMALLARSYIYNFPEAYKVYDDKGLVWNATKQDSVSIADRKQCLPK
CCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCEEECCCCCCCCHHHHHHHCHH
FDRATGNVIESYTVKDLDDQAKDKCNKLFPKGDNFVLQNNRNRLLFTFDGADGMKTGHTD
HHHCCCCHHHCCCCCCCCHHHHHHHHHCCCCCCCEEEECCCCEEEEEEECCCCCCCCCCC
AAGYCLVSSVKQDGERFISVVLGTTSSAKRDSESAKLLRYALSKYENVLLYKANSPVTIS
CHHHHHHHHHHHHHHHEEEHEEECCCCCCCCHHHHHHHHHHHHHHCEEEEEECCCCEEEE
ADNIPNAKAGQKLTVASNQNIYKTVPKTYVPYLKQGIEFNPNLNAPIKTGQNVGNLVITL
CCCCCCCCCCCEEEEECCCCHHHHCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEE
GDTKEEIASIPVVAMNNVSQKGWW
CCCHHHHHCCCEEEECCCCCCCCC
>Mature Secondary Structure
MKLTKIALLIASITAGVAFAAPNISAGSDPYFNGANGLAQKNIIIRPANIELDAPAWVTM
CCHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCCCCCCCCCCEEEECCCEEECCCEEEEE
DYRTGDIVSEKNMDVRRAPASLTKIMTSYIVASEIKAGNLSWDTMIPISENAASTGGSKM
ECCCCCEECCCCCCHHHCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCEE
YVKAGAKVSVRNLVTGMDVVSGNDATIALAEYIGGTTQAFTDLMNQTAKAIGMNNTHFAN
EEECCCEEEHHHHHHCCEEECCCCCEEEEHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCC
PDGLPGGEQYTTAHDMALLARSYIYNFPEAYKVYDDKGLVWNATKQDSVSIADRKQCLPK
CCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCEEECCCCCCCCHHHHHHHCHH
FDRATGNVIESYTVKDLDDQAKDKCNKLFPKGDNFVLQNNRNRLLFTFDGADGMKTGHTD
HHHCCCCHHHCCCCCCCCHHHHHHHHHCCCCCCCEEEECCCCEEEEEEECCCCCCCCCCC
AAGYCLVSSVKQDGERFISVVLGTTSSAKRDSESAKLLRYALSKYENVLLYKANSPVTIS
CHHHHHHHHHHHHHHHEEEHEEECCCCCCCCHHHHHHHHHHHHHHCEEEEEECCCCEEEE
ADNIPNAKAGQKLTVASNQNIYKTVPKTYVPYLKQGIEFNPNLNAPIKTGQNVGNLVITL
CCCCCCCCCCCEEEEECCCCHHHHCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEE
GDTKEEIASIPVVAMNNVSQKGWW
CCCHHHHHCCCEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8955390; 9097040; 9278503 [H]