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The map label for this gene is ispB [H]

Identifier: 89256379

GI number: 89256379

Start: 997638

End: 998615

Strand: Direct

Name: ispB [H]

Synonym: FTL_1041

Alternate gene names: 89256379

Gene position: 997638-998615 (Clockwise)

Preceding gene: 89256378

Following gene: 89256385

Centisome position: 52.62

GC content: 31.8

Gene sequence:

>978_bases
ATGCAACAGCTAAAAGATATTCAGGCACTTGTTAAAGAGGATATACAAAATAATAATCAATTCATAGTTGATTCTCTTTG
CTCAGATGTCGTACTTATCAATCAGATTAGTCACTATATAATAAATAGTGGTGGTAAAAGATTAAGACCTCTGTTAGTAA
TATTATTTGCTAGAGCCTTAAACTATAATGGAGATAAACATTTAGCATGTGCTGCAATTATTGAGTTTATTCATACAGCA
ACTCTTCTTCATGATGATGTTGTTGATGATTCGCAATTGCGTCGTGGTAAACAAACAGCAAATAATGTCTTTGGTAATGC
TGCGAGTGTTTTGACTGGAGATTTTCTCTATTCTAGAGCTTTTCAGATGATGGTAAGTTTAGATAATATGCAAATTATGC
AAATATTAGCTGATGCAACTAATAAAATCTCAGAAGGAGAGGTGCTACAACTACTAAATGCGCGTAATAGCGAACTTAGT
GAAGAAGAATATATTAAAGTGATATATTGCAAAACAGCAAAATTATTTGAAGCTGCTTGTGAATTAGCAGGTTTAATTAG
TATAGATAAACAAGATTATACTAAGTACCAAGGTAGGATTAAAAACTATGGTGTTTATTTAGGTAATGCTTTTCAGATTG
CAGATGATGTTTTAGATTATGTATCTGATGCAGAAAGTTTAGGTAAAAATATTGGTGATGATCTTGATGAAGGCAAAATG
ACTTTGCCAACAATCTACGCACTAGCTAATGTTACTGAACAACAGCAACAAATTCTAAAAAAAGCTATTGAAAAGGGCGA
GTATAGAATTGATGAAATCATAGCTATGGTCAAAGATAGTGGTGCTGTAGATTATTCTTATAAAGTGGCTTGTCAGTATG
CTGATCTCGCTAAACAACAAATAGATTTTTTACCAGATTCAGAATATAAGCAAGCTATGATTTTATTATGTGATCTAGCA
GTAAATAGGAAGAATTAA

Upstream 100 bases:

>100_bases
AAGATAATTAGTTAGATTATTTAGTAATATGTATTGATAAATGTATTCTGCTACTTTATTCTTAATCCTAATTAAGTAAA
TACTATCAATAAAATAAATA

Downstream 100 bases:

>100_bases
TTTTTATAAAAAATTTTTTATTTAAAATCTTTAAGCGTAATTTCAAAAGAAAGTGCTTGATTAGGTCCTATGACAGCGGG
CGCTCTAGTTCCATATGCCT

Product: octaprenyl-diphosphate synthase

Products: NA

Alternate protein names: All-trans-octaprenyl-diphosphate synthase; Octaprenyl pyrophosphate synthase; OPP synthase [H]

Number of amino acids: Translated: 325; Mature: 325

Protein sequence:

>325_residues
MQQLKDIQALVKEDIQNNNQFIVDSLCSDVVLINQISHYIINSGGKRLRPLLVILFARALNYNGDKHLACAAIIEFIHTA
TLLHDDVVDDSQLRRGKQTANNVFGNAASVLTGDFLYSRAFQMMVSLDNMQIMQILADATNKISEGEVLQLLNARNSELS
EEEYIKVIYCKTAKLFEAACELAGLISIDKQDYTKYQGRIKNYGVYLGNAFQIADDVLDYVSDAESLGKNIGDDLDEGKM
TLPTIYALANVTEQQQQILKKAIEKGEYRIDEIIAMVKDSGAVDYSYKVACQYADLAKQQIDFLPDSEYKQAMILLCDLA
VNRKN

Sequences:

>Translated_325_residues
MQQLKDIQALVKEDIQNNNQFIVDSLCSDVVLINQISHYIINSGGKRLRPLLVILFARALNYNGDKHLACAAIIEFIHTA
TLLHDDVVDDSQLRRGKQTANNVFGNAASVLTGDFLYSRAFQMMVSLDNMQIMQILADATNKISEGEVLQLLNARNSELS
EEEYIKVIYCKTAKLFEAACELAGLISIDKQDYTKYQGRIKNYGVYLGNAFQIADDVLDYVSDAESLGKNIGDDLDEGKM
TLPTIYALANVTEQQQQILKKAIEKGEYRIDEIIAMVKDSGAVDYSYKVACQYADLAKQQIDFLPDSEYKQAMILLCDLA
VNRKN
>Mature_325_residues
MQQLKDIQALVKEDIQNNNQFIVDSLCSDVVLINQISHYIINSGGKRLRPLLVILFARALNYNGDKHLACAAIIEFIHTA
TLLHDDVVDDSQLRRGKQTANNVFGNAASVLTGDFLYSRAFQMMVSLDNMQIMQILADATNKISEGEVLQLLNARNSELS
EEEYIKVIYCKTAKLFEAACELAGLISIDKQDYTKYQGRIKNYGVYLGNAFQIADDVLDYVSDAESLGKNIGDDLDEGKM
TLPTIYALANVTEQQQQILKKAIEKGEYRIDEIIAMVKDSGAVDYSYKVACQYADLAKQQIDFLPDSEYKQAMILLCDLA
VNRKN

Specific function: Supplies octaprenyl diphosphate, the precursor for the side chain of the isoprenoid quinones ubiquinone and menaquinone [H]

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=300, Percent_Identity=32.3333333333333, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI4758430, Length=267, Percent_Identity=28.8389513108614, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI83700220, Length=267, Percent_Identity=28.8389513108614, Blast_Score=100, Evalue=2e-21,
Organism=Escherichia coli, GI1789578, Length=322, Percent_Identity=47.8260869565217, Blast_Score=328, Evalue=2e-91,
Organism=Escherichia coli, GI1786623, Length=273, Percent_Identity=29.6703296703297, Blast_Score=91, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI17505681, Length=322, Percent_Identity=29.8136645962733, Blast_Score=145, Evalue=3e-35,
Organism=Saccharomyces cerevisiae, GI6319475, Length=305, Percent_Identity=28.8524590163934, Blast_Score=120, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6325188, Length=294, Percent_Identity=24.8299319727891, Blast_Score=91, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24651612, Length=301, Percent_Identity=28.5714285714286, Blast_Score=135, Evalue=5e-32,
Organism=Drosophila melanogaster, GI281365769, Length=265, Percent_Identity=27.5471698113208, Blast_Score=92, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24660002, Length=265, Percent_Identity=27.5471698113208, Blast_Score=92, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.90 [H]

Molecular weight: Translated: 36436; Mature: 36436

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: PS00723 POLYPRENYL_SYNTHET_1 ; PS00444 POLYPRENYL_SYNTHET_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQQLKDIQALVKEDIQNNNQFIVDSLCSDVVLINQISHYIINSGGKRLRPLLVILFARAL
CCHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
NYNGDKHLACAAIIEFIHTATLLHDDVVDDSQLRRGKQTANNVFGNAASVLTGDFLYSRA
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
FQMMVSLDNMQIMQILADATNKISEGEVLQLLNARNSELSEEEYIKVIYCKTAKLFEAAC
HHHHHHHCHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCHHHCEEEEEECHHHHHHHHH
ELAGLISIDKQDYTKYQGRIKNYGVYLGNAFQIADDVLDYVSDAESLGKNIGDDLDEGKM
HHHHHHCCCHHHHHHHHCCHHHCCEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
TLPTIYALANVTEQQQQILKKAIEKGEYRIDEIIAMVKDSGAVDYSYKVACQYADLAKQQ
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCEEHHEEHHHHHHHHHH
IDFLPDSEYKQAMILLCDLAVNRKN
HHCCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MQQLKDIQALVKEDIQNNNQFIVDSLCSDVVLINQISHYIINSGGKRLRPLLVILFARAL
CCHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
NYNGDKHLACAAIIEFIHTATLLHDDVVDDSQLRRGKQTANNVFGNAASVLTGDFLYSRA
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
FQMMVSLDNMQIMQILADATNKISEGEVLQLLNARNSELSEEEYIKVIYCKTAKLFEAAC
HHHHHHHCHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCHHHCEEEEEECHHHHHHHHH
ELAGLISIDKQDYTKYQGRIKNYGVYLGNAFQIADDVLDYVSDAESLGKNIGDDLDEGKM
HHHHHHCCCHHHHHHHHCCHHHCCEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
TLPTIYALANVTEQQQQILKKAIEKGEYRIDEIIAMVKDSGAVDYSYKVACQYADLAKQQ
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCEEHHEEHHHHHHHHHH
IDFLPDSEYKQAMILLCDLAVNRKN
HHCCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8312607; 9278503; 2670911; 8037730 [H]