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The map label for this gene is dnaB [H]

Identifier: 89256366

GI number: 89256366

Start: 984192

End: 985586

Strand: Direct

Name: dnaB [H]

Synonym: FTL_1027

Alternate gene names: 89256366

Gene position: 984192-985586 (Clockwise)

Preceding gene: 89256365

Following gene: 89256367

Centisome position: 51.91

GC content: 35.2

Gene sequence:

>1395_bases
ATGTCTATGGATTATCAGTTTAAAGCTGCTGAAACGACTTACTCTCTAGAAGCAGAAAAAGCTATCTTAGGGAATATATT
ACTGTACAATCAAAATATTGAGTTAGTTGAAGATTTTCTTTTGATTGATGATTTTTTTGATAAAAGACATAAAACCATCT
ATAAACAGATTGTCACGCTTAACCAAGCAAACACTCCGTTTGATGTACTAATTTTAAGTGAATACCTTGCCACCGAGGGA
CTTTTAGAACAAGCAGGTGGTGAGGCTTACATCATAGATTTAGCTGCTAATACACCTTCAATATCAAATATCAAGACGTA
CGCTAATATCGTAAAAGATAAGGCTAAGCTTAGAAGTTTACAAAATAGTGTAAATGATATCGTTCAGAAGATATATTCAG
CAGATTCAAAAAATCCTGATGAAGTTATTGATTACGCTGAGAGCAGAATACTTGATGTAGCTAAAGAGCGTGAAACACTT
ACCAAAGGTCCTGAATCGATAAAGTCTGTAATCCCAAAGCTTGTAGATAGAATGAGTGCTATAGTTGATTCTAGTAGTGG
TCTAACAGGGCTTTCAACAGGTTTTATAGATCTTGATAAAATGACATCAGGATTACAAAGAGCAAATATGGGTATCATAG
CAGCTCGACCATCTATGGGTAAGACTGTTTTGGGTATTAACATCGCTCAAAATGTCGCAAAAATTGCTGATAAGCCAGTA
TTAGTTTTTAGTCTAGAGATGCCGTCAGAAGATATTGTAACAAGGATGTTAGCTTCACAGGCGCGTGTAGAGATGAATTT
ATTTAAAGAATGTAATAGGTTAAATGATGCGCATTGGGTTAAAATCACCAGCGCTATGAAAACTTTAAGTGAAATGCCAC
TATATATTGATGATACATCAAGTTTGACTCCAGCCGAGATGCGTTCAAGAGCGCGAAGATTATATAATGAGCATGGTGGT
TTAGCAATGATCTTGATAGATTATCTACAGCTTATGAAAATCCCAGGTTATGAGACTAACCGAACACTAGAGGTATCAGA
GATATCCCGTTCACTGAAGGCTTTAGCCAAAGAGTTGGATATACCAGTCATAGCACTATCGCAACTAAATAGAGCAGTAG
ATGATCGTAAAGATAAACGACCAATGATGTCAGACTTAAGAGAATCAGGAGCAATTGAACAAGATGCTGACTTGATTATG
TTTATTTATCGTGATGAAGTTTATAATAAAGATAAAGAAGATAATAAAAATCTAGGTGAAATAATAATAGGTAAGCAGCG
TAATGGTCCTATAGGAACTGTGCATGTGCGCTTTGATGGTCAGTTTGCTAGTTTTGCTAATTTAACTAATGAAAATGATC
ATATTTTACCTGGTGATATAGGTTATAACGAGTAA

Upstream 100 bases:

>100_bases
TGAAGCTTAGTTTTATAATATACAAACTAACACTGTATTCTAATAAATATAAAAACCATGTGTTAGGCATGGTTTTTTTG
TATTTAAAGCAAGGGTTTTG

Downstream 100 bases:

>100_bases
TATTTATATAAAGATTTAAAGAGAAGTTTCATGCAACAAGATAAAGTTAATTTACTTGGATTAAATCAAAAAGCTATAGA
AGATTTTTTTATTTCTATAG

Product: replicative DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 464; Mature: 463

Protein sequence:

>464_residues
MSMDYQFKAAETTYSLEAEKAILGNILLYNQNIELVEDFLLIDDFFDKRHKTIYKQIVTLNQANTPFDVLILSEYLATEG
LLEQAGGEAYIIDLAANTPSISNIKTYANIVKDKAKLRSLQNSVNDIVQKIYSADSKNPDEVIDYAESRILDVAKERETL
TKGPESIKSVIPKLVDRMSAIVDSSSGLTGLSTGFIDLDKMTSGLQRANMGIIAARPSMGKTVLGINIAQNVAKIADKPV
LVFSLEMPSEDIVTRMLASQARVEMNLFKECNRLNDAHWVKITSAMKTLSEMPLYIDDTSSLTPAEMRSRARRLYNEHGG
LAMILIDYLQLMKIPGYETNRTLEVSEISRSLKALAKELDIPVIALSQLNRAVDDRKDKRPMMSDLRESGAIEQDADLIM
FIYRDEVYNKDKEDNKNLGEIIIGKQRNGPIGTVHVRFDGQFASFANLTNENDHILPGDIGYNE

Sequences:

>Translated_464_residues
MSMDYQFKAAETTYSLEAEKAILGNILLYNQNIELVEDFLLIDDFFDKRHKTIYKQIVTLNQANTPFDVLILSEYLATEG
LLEQAGGEAYIIDLAANTPSISNIKTYANIVKDKAKLRSLQNSVNDIVQKIYSADSKNPDEVIDYAESRILDVAKERETL
TKGPESIKSVIPKLVDRMSAIVDSSSGLTGLSTGFIDLDKMTSGLQRANMGIIAARPSMGKTVLGINIAQNVAKIADKPV
LVFSLEMPSEDIVTRMLASQARVEMNLFKECNRLNDAHWVKITSAMKTLSEMPLYIDDTSSLTPAEMRSRARRLYNEHGG
LAMILIDYLQLMKIPGYETNRTLEVSEISRSLKALAKELDIPVIALSQLNRAVDDRKDKRPMMSDLRESGAIEQDADLIM
FIYRDEVYNKDKEDNKNLGEIIIGKQRNGPIGTVHVRFDGQFASFANLTNENDHILPGDIGYNE
>Mature_463_residues
SMDYQFKAAETTYSLEAEKAILGNILLYNQNIELVEDFLLIDDFFDKRHKTIYKQIVTLNQANTPFDVLILSEYLATEGL
LEQAGGEAYIIDLAANTPSISNIKTYANIVKDKAKLRSLQNSVNDIVQKIYSADSKNPDEVIDYAESRILDVAKERETLT
KGPESIKSVIPKLVDRMSAIVDSSSGLTGLSTGFIDLDKMTSGLQRANMGIIAARPSMGKTVLGINIAQNVAKIADKPVL
VFSLEMPSEDIVTRMLASQARVEMNLFKECNRLNDAHWVKITSAMKTLSEMPLYIDDTSSLTPAEMRSRARRLYNEHGGL
AMILIDYLQLMKIPGYETNRTLEVSEISRSLKALAKELDIPVIALSQLNRAVDDRKDKRPMMSDLRESGAIEQDADLIMF
IYRDEVYNKDKEDNKNLGEIIIGKQRNGPIGTVHVRFDGQFASFANLTNENDHILPGDIGYNE

Specific function: Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with dnaC protein, primase, and other prepriming proteins [

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=436, Percent_Identity=48.6238532110092, Blast_Score=438, Evalue=1e-124,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 51920; Mature: 51789

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMDYQFKAAETTYSLEAEKAILGNILLYNQNIELVEDFLLIDDFFDKRHKTIYKQIVTL
CCCCCEEEECCCEEECHHHHHHHCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NQANTPFDVLILSEYLATEGLLEQAGGEAYIIDLAANTPSISNIKTYANIVKDKAKLRSL
CCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHH
QNSVNDIVQKIYSADSKNPDEVIDYAESRILDVAKERETLTKGPESIKSVIPKLVDRMSA
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
IVDSSSGLTGLSTGFIDLDKMTSGLQRANMGIIAARPSMGKTVLGINIAQNVAKIADKPV
HHCCCCCCCCCCCCCEEHHHHHHHHHHCCCCEEEECCCCCCEEEEHHHHHHHHHHCCCCE
LVFSLEMPSEDIVTRMLASQARVEMNLFKECNRLNDAHWVKITSAMKTLSEMPLYIDDTS
EEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHCCEEEECCC
SLTPAEMRSRARRLYNEHGGLAMILIDYLQLMKIPGYETNRTLEVSEISRSLKALAKELD
CCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHHCC
IPVIALSQLNRAVDDRKDKRPMMSDLRESGAIEQDADLIMFIYRDEVYNKDKEDNKNLGE
CCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCEEEEEEEHHHCCCCCCCCCCCCC
IIIGKQRNGPIGTVHVRFDGQFASFANLTNENDHILPGDIGYNE
EEEECCCCCCEEEEEEEECCCHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SMDYQFKAAETTYSLEAEKAILGNILLYNQNIELVEDFLLIDDFFDKRHKTIYKQIVTL
CCCCEEEECCCEEECHHHHHHHCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NQANTPFDVLILSEYLATEGLLEQAGGEAYIIDLAANTPSISNIKTYANIVKDKAKLRSL
CCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHH
QNSVNDIVQKIYSADSKNPDEVIDYAESRILDVAKERETLTKGPESIKSVIPKLVDRMSA
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
IVDSSSGLTGLSTGFIDLDKMTSGLQRANMGIIAARPSMGKTVLGINIAQNVAKIADKPV
HHCCCCCCCCCCCCCEEHHHHHHHHHHCCCCEEEECCCCCCEEEEHHHHHHHHHHCCCCE
LVFSLEMPSEDIVTRMLASQARVEMNLFKECNRLNDAHWVKITSAMKTLSEMPLYIDDTS
EEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHCCEEEECCC
SLTPAEMRSRARRLYNEHGGLAMILIDYLQLMKIPGYETNRTLEVSEISRSLKALAKELD
CCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHHCC
IPVIALSQLNRAVDDRKDKRPMMSDLRESGAIEQDADLIMFIYRDEVYNKDKEDNKNLGE
CCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCEEEEEEEHHHCCCCCCCCCCCCC
IIIGKQRNGPIGTVHVRFDGQFASFANLTNENDHILPGDIGYNE
EEEECCCCCCEEEEEEEECCCHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11248100 [H]