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The map label for this gene is mnmC [H]

Identifier: 89256147

GI number: 89256147

Start: 752941

End: 754146

Strand: Direct

Name: mnmC [H]

Synonym: FTL_0764

Alternate gene names: 89256147

Gene position: 752941-754146 (Clockwise)

Preceding gene: 89256146

Following gene: 89256148

Centisome position: 39.71

GC content: 29.77

Gene sequence:

>1206_bases
ATGATTAAAAGAATAGCTATTATCGGTGCTGGTCTTGCGGGATGTTCGTTAGCGTATGAGTTAAGTAAATCAGCTAGCTT
TGATATAACTATATTTGATAAGAATTCTGAGATGGCTGGTGAAGCATCAGGTAATTTTGCTGGGATATTAGCGCCATATT
TAACTTTTGATAATAACTTTTCTGATCAATTTCATACTCTTGGTTATAGATTATTGATAGATTTTATCAACCAGTATAAA
AATAACGTAGAAATTTGTAACCAAGGAGTAATCCAACTTCTAAGTGATCCAAAAGAGCAATGTAGATATCAAAAAATTTT
CATAAATAGAGATATAGATTCAAATATAGCTAAATTATTATCAGCTACACAAGTTTCTCAACTTATAAATACAACCACCT
CAAAAAGAGCTGTTTATTATCCGAATGCTTTAGCATTAGTACCTAAAAGTATTTGTCAATTATGGCTTAAATTATCAGAT
GTTGAGTTAAAGTTAGGTAATCAACTATTAAATATTCAAAGAACAGAAAATAATACTTGGCAATTAGAGTTTAATAATTT
TAAAGAAAATTTTGATATTGTTATTTTTGCAGGCGGATATCCGCTATTTAAGCAAATAGTACAACTACAAAAAATCCCAG
TATATCCATCACAAGGTCAACTAACAGTTATCGAAAAAAGCTTTGATATCACAAAAACTATTATGGATAAGGGTTATATA
ATTCCAAACTATAAGCAAAATTTGCAAGTTATTGGTGCAACTTTTCGTGATAATGGTGATACTAATGCAGATATTAGAGA
AGAGGATAATAACTTTAATATAACGCAAATAAAACAAATGCTAGATAATAGTAGTATACTGGGTATAAATATAGTTGATT
CAAGAGTAGCTACACGATGTGTGACATCAGATCATTTACCATTGGTTGGTAAATTAGTTGATTATGCTAAATTTGAACAA
GATTTTTATAAACCATTATCAAAAGGCTATCCTAAAGCAAAAATGCCAAGACTTGAATATCAGCAAGATCTCTATGTATC
TTCTGGCTTTGGTTCAAAAGGTTTGTGTTCATCATTATTAGCTGCCAAGATTATAGCTGCACAAATAACTAATCAAAAAC
TACCACACTGTGATAAATTATTAGCTGCTTTAGCAATAGAGCGTTTTTGGATTCGTGGCTTTAAAAAGGGTATAAAGTTT
TTATAA

Upstream 100 bases:

>100_bases
GTATGTTAAGAGCTGAGCAAAAGCGTAAAAAAGAAGGTATTAGACTTTTTGGAGCATAAAAGAGTTTTCAACACAGGGCT
CATACGTTAGAGAATTTTTT

Downstream 100 bases:

>100_bases
AATTATTGCTAAGCTTATTTATACACCACAGTAATTGGCTATAATAGTAAAATTTTCTAAGCTAAATTGTATAGATATGA
AGTTAAGAGTAAAAACAATC

Product: lipoprotein

Products: NA

Alternate protein names: tRNA mnm(5)s(2)U biosynthesis bifunctional protein; tRNA (mnm(5)s(2)U34)-methyltransferase; FAD-dependent cmnm(5)s(2)U34 oxidoreductase [H]

Number of amino acids: Translated: 401; Mature: 401

Protein sequence:

>401_residues
MIKRIAIIGAGLAGCSLAYELSKSASFDITIFDKNSEMAGEASGNFAGILAPYLTFDNNFSDQFHTLGYRLLIDFINQYK
NNVEICNQGVIQLLSDPKEQCRYQKIFINRDIDSNIAKLLSATQVSQLINTTTSKRAVYYPNALALVPKSICQLWLKLSD
VELKLGNQLLNIQRTENNTWQLEFNNFKENFDIVIFAGGYPLFKQIVQLQKIPVYPSQGQLTVIEKSFDITKTIMDKGYI
IPNYKQNLQVIGATFRDNGDTNADIREEDNNFNITQIKQMLDNSSILGINIVDSRVATRCVTSDHLPLVGKLVDYAKFEQ
DFYKPLSKGYPKAKMPRLEYQQDLYVSSGFGSKGLCSSLLAAKIIAAQITNQKLPHCDKLLAALAIERFWIRGFKKGIKF
L

Sequences:

>Translated_401_residues
MIKRIAIIGAGLAGCSLAYELSKSASFDITIFDKNSEMAGEASGNFAGILAPYLTFDNNFSDQFHTLGYRLLIDFINQYK
NNVEICNQGVIQLLSDPKEQCRYQKIFINRDIDSNIAKLLSATQVSQLINTTTSKRAVYYPNALALVPKSICQLWLKLSD
VELKLGNQLLNIQRTENNTWQLEFNNFKENFDIVIFAGGYPLFKQIVQLQKIPVYPSQGQLTVIEKSFDITKTIMDKGYI
IPNYKQNLQVIGATFRDNGDTNADIREEDNNFNITQIKQMLDNSSILGINIVDSRVATRCVTSDHLPLVGKLVDYAKFEQ
DFYKPLSKGYPKAKMPRLEYQQDLYVSSGFGSKGLCSSLLAAKIIAAQITNQKLPHCDKLLAALAIERFWIRGFKKGIKF
L
>Mature_401_residues
MIKRIAIIGAGLAGCSLAYELSKSASFDITIFDKNSEMAGEASGNFAGILAPYLTFDNNFSDQFHTLGYRLLIDFINQYK
NNVEICNQGVIQLLSDPKEQCRYQKIFINRDIDSNIAKLLSATQVSQLINTTTSKRAVYYPNALALVPKSICQLWLKLSD
VELKLGNQLLNIQRTENNTWQLEFNNFKENFDIVIFAGGYPLFKQIVQLQKIPVYPSQGQLTVIEKSFDITKTIMDKGYI
IPNYKQNLQVIGATFRDNGDTNADIREEDNNFNITQIKQMLDNSSILGINIVDSRVATRCVTSDHLPLVGKLVDYAKFEQ
DFYKPLSKGYPKAKMPRLEYQQDLYVSSGFGSKGLCSSLLAAKIIAAQITNQKLPHCDKLLAALAIERFWIRGFKKGIKF
L

Specific function: Catalyzes the last two steps in the biosynthesis of 5- methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at the wobble position (U34) in tRNA. Catalyzes the FAD-dependent demodification of cmnm(5)s(2)U34 to nm(5)s(2)U34, followed by the transfer of a methyl g

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the DAO family [H]

Homologues:

Organism=Escherichia coli, GI87082084, Length=406, Percent_Identity=25.8620689655172, Blast_Score=131, Evalue=1e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008471
- InterPro:   IPR006076
- InterPro:   IPR023032
- InterPro:   IPR017610 [H]

Pfam domain/function: PF01266 DAO; PF05430 DUF752 [H]

EC number: =2.1.1.61 [H]

Molecular weight: Translated: 45227; Mature: 45227

Theoretical pI: Translated: 8.90; Mature: 8.90

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKRIAIIGAGLAGCSLAYELSKSASFDITIFDKNSEMAGEASGNFAGILAPYLTFDNNF
CCEEEEEEECCHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHEECCCCC
SDQFHTLGYRLLIDFINQYKNNVEICNQGVIQLLSDPKEQCRYQKIFINRDIDSNIAKLL
CHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHCCEEEEEEECCCCHHHHHHH
SATQVSQLINTTTSKRAVYYPNALALVPKSICQLWLKLSDVELKLGNQLLNIQRTENNTW
HHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHHHHHHCCCEEECCCEEEEEEECCCCEE
QLEFNNFKENFDIVIFAGGYPLFKQIVQLQKIPVYPSQGQLTVIEKSFDITKTIMDKGYI
EEEECCCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCCCCEEEEECCCHHHHHHHHCCCC
IPNYKQNLQVIGATFRDNGDTNADIREEDNNFNITQIKQMLDNSSILGINIVDSRVATRC
CCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEEEEHHHHHHHH
VTSDHLPLVGKLVDYAKFEQDFYKPLSKGYPKAKMPRLEYQQDLYVSSGFGSKGLCSSLL
HCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCEEEECCCCCCHHHHHHH
AAKIIAAQITNQKLPHCDKLLAALAIERFWIRGFKKGIKFL
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MIKRIAIIGAGLAGCSLAYELSKSASFDITIFDKNSEMAGEASGNFAGILAPYLTFDNNF
CCEEEEEEECCHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHEECCCCC
SDQFHTLGYRLLIDFINQYKNNVEICNQGVIQLLSDPKEQCRYQKIFINRDIDSNIAKLL
CHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHCCEEEEEEECCCCHHHHHHH
SATQVSQLINTTTSKRAVYYPNALALVPKSICQLWLKLSDVELKLGNQLLNIQRTENNTW
HHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHHHHHHCCCEEECCCEEEEEEECCCCEE
QLEFNNFKENFDIVIFAGGYPLFKQIVQLQKIPVYPSQGQLTVIEKSFDITKTIMDKGYI
EEEECCCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCCCCEEEEECCCHHHHHHHHCCCC
IPNYKQNLQVIGATFRDNGDTNADIREEDNNFNITQIKQMLDNSSILGINIVDSRVATRC
CCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEEEEHHHHHHHH
VTSDHLPLVGKLVDYAKFEQDFYKPLSKGYPKAKMPRLEYQQDLYVSSGFGSKGLCSSLL
HCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCEEEECCCCCCHHHHHHH
AAKIIAAQITNQKLPHCDKLLAALAIERFWIRGFKKGIKFL
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA