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The map label for this gene is potI [H]

Identifier: 89256069

GI number: 89256069

Start: 666344

End: 667150

Strand: Reverse

Name: potI [H]

Synonym: FTL_0679

Alternate gene names: 89256069

Gene position: 667150-666344 (Counterclockwise)

Preceding gene: 89256070

Following gene: 89256065

Centisome position: 35.19

GC content: 32.84

Gene sequence:

>807_bases
ATGAAAAGATTTTCATTTAGTAAGATAATGCTTATATTAGGTTTGATCTTTTTATATATTCCACTAATAATTTTAGTGGT
TTTCTCATTTAGTAATTCTGAAATTATTAACTTATGGGGTGGTTTTACTTTTGACTGGTATTATGAGGTTATTCATGATG
AAGATATCATAAATGCCACTTTTACTAGCCTTAAAATAGCTACTATAACCTCTGCAATAGCTACAATATTAGGTACTATA
ATTGCTTATGCTATGACTAGATTTAAATTCTTTAGATCTAGAAGTTTTTTATATGGTCTTGTAGCAACACCTCTTGTATT
ACCAGATATTATTCTAGGGGTTGCTTTACTATTGATGTTTTCAACCTTACAACATTTAATTGGCTGGTCACACGAGCGTG
GTACAACTACTGTTGTGATAGCTCATGTAACTATGTGTACTGCTTATGTAACAATAGTAATGCAATCACGTATAGCTAGT
GTAGATATATCTCTAGAAGAAGCTGCTCTGGACCTAGGAGCTGGCCCTATAGAAACATTCTTTAAAATAACTCTACCACA
ACTAATACCAGCACTAATTACTTCAGCACTACTTACATTTACATTATCTATTGATGATTTAGTTGTAACAGAATTTGTTG
CAGGTAGTGATAACCCTACTTTACCGATGTATATTTACTCAACTGTAAAAAATGGTCCTACGCCTGAGATAAACGCCTTG
GCTACGATCATGATTGGAATTATCGTCGTTGGTGTGCTATTAGGCATGTTTATCTCTGCTAAGTTCCAAAAAAGACAAAA
TGGTTAA

Upstream 100 bases:

>100_bases
GCTACGATATCCGTAGTTTTAATAGTTATACTAGTTTTACCTATCCTATGGATGCAACGCATTCAAGCAAAGCGAACTTT
TGTTTAGGAGGATATGAGCG

Downstream 100 bases:

>100_bases
GATTAAAAGTTTGGTTTATATTCTAAAAGTTAAAAATAGCTTTTAAGTTTTCATTATAGTCAAATAAAAATACAGACGAA
TTTAATCAATCCTAGATATA

Product: polyamine transporter, subunit I, ABC transporter, membrane protein

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 268; Mature: 268

Protein sequence:

>268_residues
MKRFSFSKIMLILGLIFLYIPLIILVVFSFSNSEIINLWGGFTFDWYYEVIHDEDIINATFTSLKIATITSAIATILGTI
IAYAMTRFKFFRSRSFLYGLVATPLVLPDIILGVALLLMFSTLQHLIGWSHERGTTTVVIAHVTMCTAYVTIVMQSRIAS
VDISLEEAALDLGAGPIETFFKITLPQLIPALITSALLTFTLSIDDLVVTEFVAGSDNPTLPMYIYSTVKNGPTPEINAL
ATIMIGIIVVGVLLGMFISAKFQKRQNG

Sequences:

>Translated_268_residues
MKRFSFSKIMLILGLIFLYIPLIILVVFSFSNSEIINLWGGFTFDWYYEVIHDEDIINATFTSLKIATITSAIATILGTI
IAYAMTRFKFFRSRSFLYGLVATPLVLPDIILGVALLLMFSTLQHLIGWSHERGTTTVVIAHVTMCTAYVTIVMQSRIAS
VDISLEEAALDLGAGPIETFFKITLPQLIPALITSALLTFTLSIDDLVVTEFVAGSDNPTLPMYIYSTVKNGPTPEINAL
ATIMIGIIVVGVLLGMFISAKFQKRQNG
>Mature_268_residues
MKRFSFSKIMLILGLIFLYIPLIILVVFSFSNSEIINLWGGFTFDWYYEVIHDEDIINATFTSLKIATITSAIATILGTI
IAYAMTRFKFFRSRSFLYGLVATPLVLPDIILGVALLLMFSTLQHLIGWSHERGTTTVVIAHVTMCTAYVTIVMQSRIAS
VDISLEEAALDLGAGPIETFFKITLPQLIPALITSALLTFTLSIDDLVVTEFVAGSDNPTLPMYIYSTVKNGPTPEINAL
ATIMIGIIVVGVLLGMFISAKFQKRQNG

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: COG1177

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component II

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787081, Length=264, Percent_Identity=44.3181818181818, Blast_Score=224, Evalue=4e-60,
Organism=Escherichia coli, GI1787368, Length=243, Percent_Identity=35.8024691358025, Blast_Score=153, Evalue=1e-38,
Organism=Escherichia coli, GI1787714, Length=264, Percent_Identity=30.6818181818182, Blast_Score=107, Evalue=9e-25,
Organism=Escherichia coli, GI1788764, Length=231, Percent_Identity=25.1082251082251, Blast_Score=71, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 29548; Mature: 29548

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRFSFSKIMLILGLIFLYIPLIILVVFSFSNSEIINLWGGFTFDWYYEVIHDEDIINAT
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHCCCHHHHHH
FTSLKIATITSAIATILGTIIAYAMTRFKFFRSRSFLYGLVATPLVLPDIILGVALLLMF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
STLQHLIGWSHERGTTTVVIAHVTMCTAYVTIVMQSRIASVDISLEEAALDLGAGPIETF
HHHHHHHCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHH
FKITLPQLIPALITSALLTFTLSIDDLVVTEFVAGSDNPTLPMYIYSTVKNGPTPEINAL
HHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCHHHH
ATIMIGIIVVGVLLGMFISAKFQKRQNG
HHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKRFSFSKIMLILGLIFLYIPLIILVVFSFSNSEIINLWGGFTFDWYYEVIHDEDIINAT
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHCCCHHHHHH
FTSLKIATITSAIATILGTIIAYAMTRFKFFRSRSFLYGLVATPLVLPDIILGVALLLMF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
STLQHLIGWSHERGTTTVVIAHVTMCTAYVTIVMQSRIASVDISLEEAALDLGAGPIETF
HHHHHHHCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHH
FKITLPQLIPALITSALLTFTLSIDDLVVTEFVAGSDNPTLPMYIYSTVKNGPTPEINAL
HHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCHHHH
ATIMIGIIVVGVLLGMFISAKFQKRQNG
HHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]