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The map label for this gene is epsC [H]

Identifier: 89255990

GI number: 89255990

Start: 578226

End: 579962

Strand: Direct

Name: epsC [H]

Synonym: FTL_0592

Alternate gene names: 89255990

Gene position: 578226-579962 (Clockwise)

Preceding gene: 89255989

Following gene: 89255991

Centisome position: 30.5

GC content: 33.22

Gene sequence:

>1737_bases
ATGTCTTTCTACGATAATAGAACGCTTAATTTCGTGGTAATAATAGTTTTAACTATTATTACTGTTAATTGGACTTTCTA
TATTTTCAAGCAAGATGTTAATTTACATTTTTTACTTGCATTAGTTTTGCTGAGATGCTTGTCATCTTTTTTACTACTTA
GAGATTATATGGCTAGTTGGCGTAAGTCGACTCAAAAAACTTTTTTACGTAAGGCTTTTATTAATTTGCCAGTATTTTTC
ATAGTGGCATTATTTTTTTATGGCAAAGTCACTTTTTCGTTGATATTCTCTGAGTTTTTATTTTATGTTTTTTTGATCAG
TTTAAGTGTCTACTTTTATTGGTATTTGATGAACAGAGGATCAGTGGATAAAAGTAAAACTGCGGTTATTTATGGTGCAG
GTGCTGCAGGAACAAAGATTGCTCAAGAACTTGCTTCTGCTGGTTATCGCATCAAATGTTTTGTTGATGACAATGAAACT
TTACAAAAAAGAAGTATTGATAGTAAAAAGGTTCTATCTAAAGCTGAATTAACAAAACTATTGCTATCTAGTAGATTTGA
CCTTTTGGTTATTGCATTGCCAAGAAATGCAAACCAAGTAGTCAAAAATATATATAAAGAATTTGAAAAGGATTTTAATC
AGATTAGAATTATGCCGCCTCTTGAGGAAATTCTTCAAGATGAGAATTTTATGTCACAGTTGAAGCCTGTTTCACTCTAT
GATCTATTAGCGCGTGATACTAAGAGTTTAGATAAAGAATCTATCTCTAATTTTATCAAAAATAAGGTAGTGCTAGTCAC
AGGAGCTGGAGGTAGTATAGGTTCTGAAATAGTACATCAATGTATCAAGTATCAGGCAAAAGAGTTGATATTGGTTGATC
ATAGTGAGTTTAACTTATATAAAATTACTGAGGAGTGTAGTCATTTTAATATCAATAGTGTGCTATGTTCTGTTTGTGAT
AGAAAAGCATTGGCTGAGGTTTTTCAAAAGTATACTCCAAATATAGTATTTCATGCTGCTGCCTACAAGCATGTTCCCTT
AGTTGAGGAGAATATCTCTAGAGCAATTAGAAATAATATCTTAGGTACTAAGAATGCTATAGATCTGGCTATAGAAGCTG
GTGTTGAGTCATTTATATTGATTTCCACTGATAAAGCAGTGCGACCAACGAATGTTATGGGGGCTACCAAGAGAGTTTGT
GAGCTGTATTTACAGAATGTTGATCCCAAAAATACCAAGCTTGCTGCAGTGCGTTTTGGTAATGTGCTTGGTAGTAGTGG
CAGTGTGATTCCAAAATTTGAAGAGCAAATAAGAAATGGTGGTCCTGTTACAGTTACTCATCCTGAAATTACACGTTATT
TTATGTTGATACCAGAAGCTTGTGAACTGGTCCTACAAGCTGGTGCTATTGCAAAAAATTCAGAGGTCTTTGTCTTAGAT
ATGGGGCAACCTGTCAAGATTATTGATCTTGCTAAACAATTTATTAGACTTTCTGGTAGAGGTGATATTGATATTAAAAT
AGTTGGTTTGCGTCCAGGAGAGAAACTTTACGAAGAGCTTTTGATAGAGGAAGATGATGTTAGTACCGACTATAAAGATA
TTTTTATTGGTAGAAGGACTTTTTACGATATTAATACTCTAAACCAAGATATTGAATCGTTGATCAAGGATGATGTTGAT
CAGCTTGTGATATTAAAGAAAATTGTTCCGGAATTTGAACATAGATTGAATGGGTAG

Upstream 100 bases:

>100_bases
AACTATTTTTGTGCACAGAACCTAATTTGCATTTTTGTGCACAAAGAAAATTTTTTTTGATATAATAGACTCTAATAGGA
TATTTTCTAAAAATTAACAA

Downstream 100 bases:

>100_bases
TGGTTTTATGTTTTATAAGGTTTTTAAAAGATTGCTTGATATTTTACTTTCTTTTATGGGGTTGTTGTTATTAAGTCCTA
TTTTCTTAATTATTATTTTT

Product: dTDP-glucose 4,6-dehydratase

Products: dTDP-4-dehydro-6-deoxy-D-glucose; H2O

Alternate protein names: NA

Number of amino acids: Translated: 578; Mature: 577

Protein sequence:

>578_residues
MSFYDNRTLNFVVIIVLTIITVNWTFYIFKQDVNLHFLLALVLLRCLSSFLLLRDYMASWRKSTQKTFLRKAFINLPVFF
IVALFFYGKVTFSLIFSEFLFYVFLISLSVYFYWYLMNRGSVDKSKTAVIYGAGAAGTKIAQELASAGYRIKCFVDDNET
LQKRSIDSKKVLSKAELTKLLLSSRFDLLVIALPRNANQVVKNIYKEFEKDFNQIRIMPPLEEILQDENFMSQLKPVSLY
DLLARDTKSLDKESISNFIKNKVVLVTGAGGSIGSEIVHQCIKYQAKELILVDHSEFNLYKITEECSHFNINSVLCSVCD
RKALAEVFQKYTPNIVFHAAAYKHVPLVEENISRAIRNNILGTKNAIDLAIEAGVESFILISTDKAVRPTNVMGATKRVC
ELYLQNVDPKNTKLAAVRFGNVLGSSGSVIPKFEEQIRNGGPVTVTHPEITRYFMLIPEACELVLQAGAIAKNSEVFVLD
MGQPVKIIDLAKQFIRLSGRGDIDIKIVGLRPGEKLYEELLIEEDDVSTDYKDIFIGRRTFYDINTLNQDIESLIKDDVD
QLVILKKIVPEFEHRLNG

Sequences:

>Translated_578_residues
MSFYDNRTLNFVVIIVLTIITVNWTFYIFKQDVNLHFLLALVLLRCLSSFLLLRDYMASWRKSTQKTFLRKAFINLPVFF
IVALFFYGKVTFSLIFSEFLFYVFLISLSVYFYWYLMNRGSVDKSKTAVIYGAGAAGTKIAQELASAGYRIKCFVDDNET
LQKRSIDSKKVLSKAELTKLLLSSRFDLLVIALPRNANQVVKNIYKEFEKDFNQIRIMPPLEEILQDENFMSQLKPVSLY
DLLARDTKSLDKESISNFIKNKVVLVTGAGGSIGSEIVHQCIKYQAKELILVDHSEFNLYKITEECSHFNINSVLCSVCD
RKALAEVFQKYTPNIVFHAAAYKHVPLVEENISRAIRNNILGTKNAIDLAIEAGVESFILISTDKAVRPTNVMGATKRVC
ELYLQNVDPKNTKLAAVRFGNVLGSSGSVIPKFEEQIRNGGPVTVTHPEITRYFMLIPEACELVLQAGAIAKNSEVFVLD
MGQPVKIIDLAKQFIRLSGRGDIDIKIVGLRPGEKLYEELLIEEDDVSTDYKDIFIGRRTFYDINTLNQDIESLIKDDVD
QLVILKKIVPEFEHRLNG
>Mature_577_residues
SFYDNRTLNFVVIIVLTIITVNWTFYIFKQDVNLHFLLALVLLRCLSSFLLLRDYMASWRKSTQKTFLRKAFINLPVFFI
VALFFYGKVTFSLIFSEFLFYVFLISLSVYFYWYLMNRGSVDKSKTAVIYGAGAAGTKIAQELASAGYRIKCFVDDNETL
QKRSIDSKKVLSKAELTKLLLSSRFDLLVIALPRNANQVVKNIYKEFEKDFNQIRIMPPLEEILQDENFMSQLKPVSLYD
LLARDTKSLDKESISNFIKNKVVLVTGAGGSIGSEIVHQCIKYQAKELILVDHSEFNLYKITEECSHFNINSVLCSVCDR
KALAEVFQKYTPNIVFHAAAYKHVPLVEENISRAIRNNILGTKNAIDLAIEAGVESFILISTDKAVRPTNVMGATKRVCE
LYLQNVDPKNTKLAAVRFGNVLGSSGSVIPKFEEQIRNGGPVTVTHPEITRYFMLIPEACELVLQAGAIAKNSEVFVLDM
GQPVKIIDLAKQFIRLSGRGDIDIKIVGLRPGEKLYEELLIEEDDVSTDYKDIFIGRRTFYDINTLNQDIESLIKDDVDQ
LVILKKIVPEFEHRLNG

Specific function: Involved in biofilm formation [H]

COG id: COG1086

COG function: function code MG; Predicted nucleoside-diphosphate sugar epimerases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polysaccharide synthase family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6319493, Length=206, Percent_Identity=31.0679611650485, Blast_Score=65, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR003869 [H]

Pfam domain/function: PF02719 Polysacc_synt_2 [H]

EC number: 4.2.1.46

Molecular weight: Translated: 65710; Mature: 65579

Theoretical pI: Translated: 8.17; Mature: 8.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFYDNRTLNFVVIIVLTIITVNWTFYIFKQDVNLHFLLALVLLRCLSSFLLLRDYMASW
CCCCCCCCHHHHHHHHHHHHHHCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHH
RKSTQKTFLRKAFINLPVFFIVALFFYGKVTFSLIFSEFLFYVFLISLSVYFYWYLMNRG
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
SVDKSKTAVIYGAGAAGTKIAQELASAGYRIKCFVDDNETLQKRSIDSKKVLSKAELTKL
CCCCCCEEEEEECCCCHHHHHHHHHHCCCEEEEEECCCHHHHHHCCCHHHHHHHHHHHHH
LLSSRFDLLVIALPRNANQVVKNIYKEFEKDFNQIRIMPPLEEILQDENFMSQLKPVSLY
HHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHEEECCCHHHHHCCCHHHHHCCCHHHH
DLLARDTKSLDKESISNFIKNKVVLVTGAGGSIGSEIVHQCIKYQAKELILVDHSEFNLY
HHHHHHHHHCCHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHCCCEEEEEECCCCCEE
KITEECSHFNINSVLCSVCDRKALAEVFQKYTPNIVFHAAAYKHVPLVEENISRAIRNNI
EEHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHCCCCCHHHHHHHHHHHCC
LGTKNAIDLAIEAGVESFILISTDKAVRPTNVMGATKRVCELYLQNVDPKNTKLAAVRFG
CCCCCHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEEC
NVLGSSGSVIPKFEEQIRNGGPVTVTHPEITRYFMLIPEACELVLQAGAIAKNSEVFVLD
HHHCCCCCCCCHHHHHHCCCCCEEEECHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEE
MGQPVKIIDLAKQFIRLSGRGDIDIKIVGLRPGEKLYEELLIEEDDVSTDYKDIFIGRRT
CCCCCHHHHHHHHHHHHCCCCCEEEEEEEECCCHHHHHHHCCCCCCCCCCHHHHEECCHH
FYDINTLNQDIESLIKDDVDQLVILKKIVPEFEHRLNG
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SFYDNRTLNFVVIIVLTIITVNWTFYIFKQDVNLHFLLALVLLRCLSSFLLLRDYMASW
CCCCCCCHHHHHHHHHHHHHHCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHH
RKSTQKTFLRKAFINLPVFFIVALFFYGKVTFSLIFSEFLFYVFLISLSVYFYWYLMNRG
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
SVDKSKTAVIYGAGAAGTKIAQELASAGYRIKCFVDDNETLQKRSIDSKKVLSKAELTKL
CCCCCCEEEEEECCCCHHHHHHHHHHCCCEEEEEECCCHHHHHHCCCHHHHHHHHHHHHH
LLSSRFDLLVIALPRNANQVVKNIYKEFEKDFNQIRIMPPLEEILQDENFMSQLKPVSLY
HHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHEEECCCHHHHHCCCHHHHHCCCHHHH
DLLARDTKSLDKESISNFIKNKVVLVTGAGGSIGSEIVHQCIKYQAKELILVDHSEFNLY
HHHHHHHHHCCHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHCCCEEEEEECCCCCEE
KITEECSHFNINSVLCSVCDRKALAEVFQKYTPNIVFHAAAYKHVPLVEENISRAIRNNI
EEHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHCCCCCHHHHHHHHHHHCC
LGTKNAIDLAIEAGVESFILISTDKAVRPTNVMGATKRVCELYLQNVDPKNTKLAAVRFG
CCCCCHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEEC
NVLGSSGSVIPKFEEQIRNGGPVTVTHPEITRYFMLIPEACELVLQAGAIAKNSEVFVLD
HHHCCCCCCCCHHHHHHCCCCCEEEECHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEE
MGQPVKIIDLAKQFIRLSGRGDIDIKIVGLRPGEKLYEELLIEEDDVSTDYKDIFIGRRT
CCCCCHHHHHHHHHHHHCCCCCEEEEEEEECCCHHHHHHHCCCCCCCCCCHHHHEECCHH
FYDINTLNQDIESLIKDDVDQLVILKKIVPEFEHRLNG
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.093 {dTDPglucose}} [C]

Substrates: dTDPglucose

Specific reaction: dTDP-glucose = dTDP-4-dehydro-6-deoxy-D-glucose + H2O

General reaction: Elimination (of H2O C-O bond cleavage [C]

Inhibitor: p-Chloromercuribenzoate; TMP [C]

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969506; 9384377 [H]