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The map label for this gene is waaA [H]

Identifier: 89255953

GI number: 89255953

Start: 529649

End: 530944

Strand: Reverse

Name: waaA [H]

Synonym: FTL_0547

Alternate gene names: 89255953

Gene position: 530944-529649 (Counterclockwise)

Preceding gene: 89255954

Following gene: 89255952

Centisome position: 28.0

GC content: 30.79

Gene sequence:

>1296_bases
GTGGAACATTTAAAAAAATTCTTTTATGTTTTTCTAGCTCATATATATTCGAGCATTTTTATAACATTAATACCTATTTT
ATATCTGAAAAAATTTAAAAGAAGCTTTAAAAACATTAACTACAGAAAAAGATGGGCAGAGAGATTCGCCCAGATACCAA
TACGCCTTAACAGCAGTATATGGATACATTCAGTTTCTGTAGGTGAAGCTGTCTCTGCTGAACCACTAGTAAAAGAATTA
CTAAAGAATTTTCCTAATGAGAATTTTGTTATAACTACAACAACACCAACAGGTAGCGATGTTGTGAATAGTCTTTATAG
TAACTATCCCAATGTACACCATATGTATATCCCTTATGATGTAATTCCTTTTATTAATAGTTTTTTTGCTAAGACTAATC
CAAAGATTTTTATAATCGTTGAAACAGAAATTTGGCCAAATATTCTAAATAAGTGTTTTGCTGAAAAGGTTCCAGTAGTA
ATAACAAATGCGCGACTATCAAAAAAATCGATGCGTAATTACACTAAAATCCCTTTTGCTAAAGAATTTTTATTTAAGAA
TATCTCTCATATTAACGCTCAGACAGAGAAAGATGCAAAAAGATTTTATTCTCTAGCTGTAGATAAAAACAACATTTCTG
TGACAGGAAACTTAAAATACAATCTTATCACTCCAGAAAACCTAGAAAATAAGATGTATAGCTTGAAAGACAGCCTAAAA
GGTAGACCAGTATGGATTGCAGGGAGTACTCATCAAGGTGAGGAAGAAATAATTCTTGAAGCACATAAACAAATCCTAAA
AATACATACAGATTGTTTATTAATACTAGTTCCTAGGCACAAAGAACGCTTTCAGAAAGTAGAAAAGCTAATTGTTTATA
ATTCTTTGAAGTATCAAAAAAGAAGCTCTTTTGAATGTCAAATCTTTAGTGATACTCAAGTATATTTAGGCGATACAATG
GGCGAACTACTTCATCTTTATTATATTGCAGATATAACTTTTGTTGGTGGAAGTTTAATAGACAATGGTGGACATAATTT
ACTTGAACCAGCAGCATTAGCAAAGCCAATTTTAAGTGGTCCAAGCCTTTTTAATTTCAGCCAAATATCCAAAGAACTAA
TTCGTAATAAAGCTCTAATTCGCATAAGAAACCAACAAGAAATTGCAAATAATATCTTCAAAATACTAGAAGATAAACAA
CTATTACAACAGATGTCATCGGGCGCTCTTAAGACTTTTAAAAGCCATAGTGATGTACTCGAAAAACAGTATAACAATAT
CGTAAAATTCTTATGA

Upstream 100 bases:

>100_bases
CTAAATTTTACTAATTCACATTAGTTAAATACCGTTGTTATATGTTACAATGTATCAACAAGTGTCGCCAAGTTTATAAT
TAATAGTTTTAGGTTAAAGA

Downstream 100 bases:

>100_bases
GTAGTGAAAATATAATAAAAGATTTCGAAATTTTTGCTGAAAAGGTATTTGATCAACTTAACTGTGTATCCAAAACTTTA
CTAAGTGCAATGCACTATAG

Product: 3-deoxy-D-manno-octulosonic-acid transferase

Products: KDO2-lipid IVA; CMP; KDO1-lipid IVA [C]

Alternate protein names: KDO transferase [H]

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MEHLKKFFYVFLAHIYSSIFITLIPILYLKKFKRSFKNINYRKRWAERFAQIPIRLNSSIWIHSVSVGEAVSAEPLVKEL
LKNFPNENFVITTTTPTGSDVVNSLYSNYPNVHHMYIPYDVIPFINSFFAKTNPKIFIIVETEIWPNILNKCFAEKVPVV
ITNARLSKKSMRNYTKIPFAKEFLFKNISHINAQTEKDAKRFYSLAVDKNNISVTGNLKYNLITPENLENKMYSLKDSLK
GRPVWIAGSTHQGEEEIILEAHKQILKIHTDCLLILVPRHKERFQKVEKLIVYNSLKYQKRSSFECQIFSDTQVYLGDTM
GELLHLYYIADITFVGGSLIDNGGHNLLEPAALAKPILSGPSLFNFSQISKELIRNKALIRIRNQQEIANNIFKILEDKQ
LLQQMSSGALKTFKSHSDVLEKQYNNIVKFL

Sequences:

>Translated_431_residues
MEHLKKFFYVFLAHIYSSIFITLIPILYLKKFKRSFKNINYRKRWAERFAQIPIRLNSSIWIHSVSVGEAVSAEPLVKEL
LKNFPNENFVITTTTPTGSDVVNSLYSNYPNVHHMYIPYDVIPFINSFFAKTNPKIFIIVETEIWPNILNKCFAEKVPVV
ITNARLSKKSMRNYTKIPFAKEFLFKNISHINAQTEKDAKRFYSLAVDKNNISVTGNLKYNLITPENLENKMYSLKDSLK
GRPVWIAGSTHQGEEEIILEAHKQILKIHTDCLLILVPRHKERFQKVEKLIVYNSLKYQKRSSFECQIFSDTQVYLGDTM
GELLHLYYIADITFVGGSLIDNGGHNLLEPAALAKPILSGPSLFNFSQISKELIRNKALIRIRNQQEIANNIFKILEDKQ
LLQQMSSGALKTFKSHSDVLEKQYNNIVKFL
>Mature_431_residues
MEHLKKFFYVFLAHIYSSIFITLIPILYLKKFKRSFKNINYRKRWAERFAQIPIRLNSSIWIHSVSVGEAVSAEPLVKEL
LKNFPNENFVITTTTPTGSDVVNSLYSNYPNVHHMYIPYDVIPFINSFFAKTNPKIFIIVETEIWPNILNKCFAEKVPVV
ITNARLSKKSMRNYTKIPFAKEFLFKNISHINAQTEKDAKRFYSLAVDKNNISVTGNLKYNLITPENLENKMYSLKDSLK
GRPVWIAGSTHQGEEEIILEAHKQILKIHTDCLLILVPRHKERFQKVEKLIVYNSLKYQKRSSFECQIFSDTQVYLGDTM
GELLHLYYIADITFVGGSLIDNGGHNLLEPAALAKPILSGPSLFNFSQISKELIRNKALIRIRNQQEIANNIFKILEDKQ
LLQQMSSGALKTFKSHSDVLEKQYNNIVKFL

Specific function: Essential step in lipopolysaccharides biosynthesis. Acts at transfer of 3-deoxy-D-mono octulonic acid (KDO) from CMP-KDO to a tetraacyldisaccharide 1,4'-bisphosphate precursor of lipid A (lipid IVA). Transfers two molecules of KDO to lipid IVA [H]

COG id: COG1519

COG function: function code M; 3-deoxy-D-manno-octulosonic-acid transferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1790064, Length=387, Percent_Identity=41.8604651162791, Blast_Score=316, Evalue=2e-87,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296
- InterPro:   IPR007507 [H]

Pfam domain/function: PF00534 Glycos_transf_1; PF04413 Glycos_transf_N [H]

EC number: 2.-.-.- [C]

Molecular weight: Translated: 49862; Mature: 49862

Theoretical pI: Translated: 10.02; Mature: 10.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEHLKKFFYVFLAHIYSSIFITLIPILYLKKFKRSFKNINYRKRWAERFAQIPIRLNSSI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCEEECCEE
WIHSVSVGEAVSAEPLVKELLKNFPNENFVITTTTPTGSDVVNSLYSNYPNVHHMYIPYD
EEEEECCCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHCCCCCEEEEECHH
VIPFINSFFAKTNPKIFIIVETEIWPNILNKCFAEKVPVVITNARLSKKSMRNYTKIPFA
HHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHCCCCHH
KEFLFKNISHINAQTEKDAKRFYSLAVDKNNISVTGNLKYNLITPENLENKMYSLKDSLK
HHHHHHHHHHCCCCCHHHHHHHHHHEEECCCEEEEECEEEEEECCCCHHHHHHHHHHHCC
GRPVWIAGSTHQGEEEIILEAHKQILKIHTDCLLILVPRHKERFQKVEKLIVYNSLKYQK
CCEEEEECCCCCCHHHHHHHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHHHHHCCCHHC
RSSFECQIFSDTQVYLGDTMGELLHLYYIADITFVGGSLIDNGGHNLLEPAALAKPILSG
CCCCEEEEECCCEEEHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCCCHHHHHHHHHCC
PSLFNFSQISKELIRNKALIRIRNQQEIANNIFKILEDKQLLQQMSSGALKTFKSHSDVL
CCCCCHHHHHHHHHCCCHHEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EKQYNNIVKFL
HHHHHHHHHCC
>Mature Secondary Structure
MEHLKKFFYVFLAHIYSSIFITLIPILYLKKFKRSFKNINYRKRWAERFAQIPIRLNSSI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCEEECCEE
WIHSVSVGEAVSAEPLVKELLKNFPNENFVITTTTPTGSDVVNSLYSNYPNVHHMYIPYD
EEEEECCCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHCCCCCEEEEECHH
VIPFINSFFAKTNPKIFIIVETEIWPNILNKCFAEKVPVVITNARLSKKSMRNYTKIPFA
HHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHCCCCHH
KEFLFKNISHINAQTEKDAKRFYSLAVDKNNISVTGNLKYNLITPENLENKMYSLKDSLK
HHHHHHHHHHCCCCCHHHHHHHHHHEEECCCEEEEECEEEEEECCCCHHHHHHHHHHHCC
GRPVWIAGSTHQGEEEIILEAHKQILKIHTDCLLILVPRHKERFQKVEKLIVYNSLKYQK
CCEEEEECCCCCCHHHHHHHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHHHHHCCCHHC
RSSFECQIFSDTQVYLGDTMGELLHLYYIADITFVGGSLIDNGGHNLLEPAALAKPILSG
CCCCEEEEECCCEEEHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCCCHHHHHHHHHCC
PSLFNFSQISKELIRNKALIRIRNQQEIANNIFKILEDKQLLQQMSSGALKTFKSHSDVL
CCCCCHHHHHHHHHCCCHHEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EKQYNNIVKFL
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: KDO1-lipid IVA; CMP-3-deoxy-D-manno-octulosonate; lipid IVA [C]

Specific reaction: KDO1-lipid IVA + CMP-3-deoxy-D-manno-octulosonate = KDO2-lipid IVA + CMP CMP-3-deoxy-D-manno-octulosonate + lipid IVA = KDO1-lipid IVA + CMP [C]

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]