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The map label for this gene is yafC [C]

Identifier: 89054666

GI number: 89054666

Start: 2180867

End: 2181781

Strand: Reverse

Name: yafC [C]

Synonym: Jann_2175

Alternate gene names: 89054666

Gene position: 2181781-2180867 (Counterclockwise)

Preceding gene: 89054670

Following gene: 89054665

Centisome position: 50.53

GC content: 60.66

Gene sequence:

>915_bases
ATGGATTGGGACAAGTTGCGCATCTTTCATGCGGTGGCCTCGGCGGGGTCCCTGACCCATGCGGGCGATACGCTGCATCT
CAGCCAATCCGCCGTGTCTCGTCAGATCCGCGCGCTGGAGGAAGGCCTTGATACAACGCTGTTTCACCGCCATGCGCGGG
GTCTGATCCTGACGGAACAGGGTGAGTTGTTGTTTGATGCCACACGCCACATGACCAAGCGCCTTGATGCCGCGTCTGCG
CGCATTCGCGACAGTGAGGAAGAGGTCTTTGGCGAGCTGCGGGTGACGACGACCATTGGCTTCGGCTCCCTCTGGCTGGC
CCCGCGTCTGCCCGCCCTTTACGAGAAATACCCCGATCTCAAGATCGACCTGATGCTGGAAGAACGCCTGCTGGATCTGC
CGATGCGCGAGGCCGACGTGGCCATCCGCATGAAAGAGCCGTCGCAGGCCGACCTGATCCGCCGCCGCCTGATGACGATC
AACATGCGTCTTTATGCGACGCCCTCCTACATCGAAAAGCACGGGATGCCCGACAGCATTCACGCCTTGGGTAATCATCG
CCTGATCTCACAGAACGCGGCGGCCGCACAGGTCAGCGCGGGGGCCATTCTGGTGCGCGAACTGCTCAGCTATGATGTGG
GCTCGACCCTTGCGGTGAACAATTATTTCGGTGTCCTGCAGGCCGTCATCCATGATCTTGGTATCGGGGTGTTGCCTGAT
TATCTAATCCAGGATTTCCCCGATCTGGTCCGGGTGCTGCCGGAGGTTGAATCCGCAGAAGTGCCGGTCTTCCTCGCCTA
CCCCGAGGAGTTGCGCCAATCCCGCCGGATCGAGGTGTTCCGCGATTTTGTCAGCGAAGAGGTCATCGCCTATCGCCGCC
GACAACGGGAGGCTGAGGCGATTACGGCTGCCTAA

Upstream 100 bases:

>100_bases
GTCTTCATCCGCCGGTAACCCGCATCTGTCAGAAAGCCTTTGCACCTGCAGCATGGCCCCCTTACGGTGCGCTCTGCACG
CATTTATTTGAGGTTTTACG

Downstream 100 bases:

>100_bases
ACGATAGGCAAATGACACCTTTGGATGTGTGCTATGCGCCACAGACATACCTGCTTTGCAGACGCAAGCCCTGATTTGCC
TTGATCGGAAGCACTTAGCC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 304; Mature: 304

Protein sequence:

>304_residues
MDWDKLRIFHAVASAGSLTHAGDTLHLSQSAVSRQIRALEEGLDTTLFHRHARGLILTEQGELLFDATRHMTKRLDAASA
RIRDSEEEVFGELRVTTTIGFGSLWLAPRLPALYEKYPDLKIDLMLEERLLDLPMREADVAIRMKEPSQADLIRRRLMTI
NMRLYATPSYIEKHGMPDSIHALGNHRLISQNAAAAQVSAGAILVRELLSYDVGSTLAVNNYFGVLQAVIHDLGIGVLPD
YLIQDFPDLVRVLPEVESAEVPVFLAYPEELRQSRRIEVFRDFVSEEVIAYRRRQREAEAITAA

Sequences:

>Translated_304_residues
MDWDKLRIFHAVASAGSLTHAGDTLHLSQSAVSRQIRALEEGLDTTLFHRHARGLILTEQGELLFDATRHMTKRLDAASA
RIRDSEEEVFGELRVTTTIGFGSLWLAPRLPALYEKYPDLKIDLMLEERLLDLPMREADVAIRMKEPSQADLIRRRLMTI
NMRLYATPSYIEKHGMPDSIHALGNHRLISQNAAAAQVSAGAILVRELLSYDVGSTLAVNNYFGVLQAVIHDLGIGVLPD
YLIQDFPDLVRVLPEVESAEVPVFLAYPEELRQSRRIEVFRDFVSEEVIAYRRRQREAEAITAA
>Mature_304_residues
MDWDKLRIFHAVASAGSLTHAGDTLHLSQSAVSRQIRALEEGLDTTLFHRHARGLILTEQGELLFDATRHMTKRLDAASA
RIRDSEEEVFGELRVTTTIGFGSLWLAPRLPALYEKYPDLKIDLMLEERLLDLPMREADVAIRMKEPSQADLIRRRLMTI
NMRLYATPSYIEKHGMPDSIHALGNHRLISQNAAAAQVSAGAILVRELLSYDVGSTLAVNNYFGVLQAVIHDLGIGVLPD
YLIQDFPDLVRVLPEVESAEVPVFLAYPEELRQSRRIEVFRDFVSEEVIAYRRRQREAEAITAA

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=289, Percent_Identity=29.4117647058824, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI1787128, Length=300, Percent_Identity=27.3333333333333, Blast_Score=98, Evalue=6e-22,
Organism=Escherichia coli, GI1789440, Length=293, Percent_Identity=29.3515358361775, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI145693193, Length=291, Percent_Identity=25.4295532646048, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI87081978, Length=177, Percent_Identity=31.638418079096, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI157672245, Length=140, Percent_Identity=37.1428571428571, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1787589, Length=186, Percent_Identity=29.0322580645161, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI1789639, Length=182, Percent_Identity=26.9230769230769, Blast_Score=78, Evalue=9e-16,
Organism=Escherichia coli, GI1789173, Length=139, Percent_Identity=33.8129496402878, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1786448, Length=296, Percent_Identity=24.6621621621622, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788706, Length=197, Percent_Identity=26.9035532994924, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1787601, Length=175, Percent_Identity=27.4285714285714, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1788297, Length=143, Percent_Identity=27.972027972028, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34326; Mature: 34326

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDWDKLRIFHAVASAGSLTHAGDTLHLSQSAVSRQIRALEEGLDTTLFHRHARGLILTEQ
CCHHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHHHCCHHHHHHHHHCCEEEECC
GELLFDATRHMTKRLDAASARIRDSEEEVFGELRVTTTIGFGSLWLAPRLPALYEKYPDL
CCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHEEEEEEECCCHHHHCCCCCHHHHHCCCC
KIDLMLEERLLDLPMREADVAIRMKEPSQADLIRRRLMTINMRLYATPSYIEKHGMPDSI
EEEHEEHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHEEEEEECHHHHHHCCCCHHH
HALGNHRLISQNAAAAQVSAGAILVRELLSYDVGSTLAVNNYFGVLQAVIHDLGIGVLPD
HHHCCCEEECCCCHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHCCCCCCHH
YLIQDFPDLVRVLPEVESAEVPVFLAYPEELRQSRRIEVFRDFVSEEVIAYRRRQREAEA
HHHHHHHHHHHHHCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ITAA
HCCC
>Mature Secondary Structure
MDWDKLRIFHAVASAGSLTHAGDTLHLSQSAVSRQIRALEEGLDTTLFHRHARGLILTEQ
CCHHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHHHCCHHHHHHHHHCCEEEECC
GELLFDATRHMTKRLDAASARIRDSEEEVFGELRVTTTIGFGSLWLAPRLPALYEKYPDL
CCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHEEEEEEECCCHHHHCCCCCHHHHHCCCC
KIDLMLEERLLDLPMREADVAIRMKEPSQADLIRRRLMTINMRLYATPSYIEKHGMPDSI
EEEHEEHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHEEEEEECHHHHHHCCCCHHH
HALGNHRLISQNAAAAQVSAGAILVRELLSYDVGSTLAVNNYFGVLQAVIHDLGIGVLPD
HHHCCCEEECCCCHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHCCCCCCHH
YLIQDFPDLVRVLPEVESAEVPVFLAYPEELRQSRRIEVFRDFVSEEVIAYRRRQREAEA
HHHHHHHHHHHHHCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ITAA
HCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8635745; 7542800 [H]