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The map label for this gene is eda [H]

Identifier: 89054445

GI number: 89054445

Start: 1947888

End: 1948529

Strand: Direct

Name: eda [H]

Synonym: Jann_1954

Alternate gene names: 89054445

Gene position: 1947888-1948529 (Clockwise)

Preceding gene: 89054444

Following gene: 89054446

Centisome position: 45.11

GC content: 66.51

Gene sequence:

>642_bases
ATGACCCCCCAAGCCCAATCCCTTGCCGCCCATCGCATCGCCGCCCTTGCGCCGATCATCCCCGTCCTCGTGGTCAATGA
TCCCGCCCATGCGCAGCCATTGGCCTCGGCTCTGGTCAAAGGCGGGTTGCCCGCGTTGGAAGTCACCCTTCGCACCCCCT
GCGCGCTGGAGGTCATCGCAGAGATGGCGAAGGTCGAAGGCGGCGTCGTGGGTGCGGGCACGCTCCTCACCCCCCGGGAT
GTGGAAAACGCCAAGGAGGCCGGCGCCGTATTCGGCGTCTCGCCGGGGGCCACGGCGCATCTGATTGATGCCTGTGCGGC
CAATGAATTGCCCTTGCTGCCGGGGGCGGCCACGGCGTCAGAGGTCATGTTTCTGTTCGAGCAAGGCTTTGATCTCCTGA
AGTTCTTTCCGGCCGAGGCCAATGGCGGCGTGCCTGCCCTCAAGGCGATCGGCGCACCGATCCCGCAGGTCTCGTTCTGC
CCCACGGGCGGTGTCAGCATCTCCAACGCGCCTGACTATCTTGGCCTGCCCAACGTGGTCTGCGCCGGCGGCTCTTGGGT
TGCGCCGAACGCGGCTGTTGAGGCGGGCGATTGGGACCGGATTGAGCAATTGGCCAGTGAGGCGGCGGCGCTCTCGCGTT
GA

Upstream 100 bases:

>100_bases
TAAATACAACTCAATCCGACCCTTCCAAACCGTCACTTCCGCCAGACGGCGCGCTCCAGACCTCACCGCCCGCGCCTCTG
CCCGCTGATCAGGACCTCCC

Downstream 100 bases:

>100_bases
TTGGCCGGGTCGCGGCGCGGACGTGGCGGCTTCTGGCCGATCTTTGGCAGAGCGATCAGCACATTTTGGCCAAGCGGGCC
GCGCTCACAAACAGGTGAAG

Product: 2-keto-3-deoxy-phosphogluconate aldolase

Products: NA

Alternate protein names: 4-hydroxy-2-oxoglutarate aldolase; 2-keto-4-hydroxyglutarate aldolase; KHG-aldolase; 2-dehydro-3-deoxy-phosphogluconate aldolase; 2-keto-3-deoxy-6-phosphogluconate aldolase; KDPG-aldolase; Phospho-2-dehydro-3-deoxygluconate aldolase; Phospho-2-keto-3-deoxygluconate aldolase [H]

Number of amino acids: Translated: 213; Mature: 212

Protein sequence:

>213_residues
MTPQAQSLAAHRIAALAPIIPVLVVNDPAHAQPLASALVKGGLPALEVTLRTPCALEVIAEMAKVEGGVVGAGTLLTPRD
VENAKEAGAVFGVSPGATAHLIDACAANELPLLPGAATASEVMFLFEQGFDLLKFFPAEANGGVPALKAIGAPIPQVSFC
PTGGVSISNAPDYLGLPNVVCAGGSWVAPNAAVEAGDWDRIEQLASEAAALSR

Sequences:

>Translated_213_residues
MTPQAQSLAAHRIAALAPIIPVLVVNDPAHAQPLASALVKGGLPALEVTLRTPCALEVIAEMAKVEGGVVGAGTLLTPRD
VENAKEAGAVFGVSPGATAHLIDACAANELPLLPGAATASEVMFLFEQGFDLLKFFPAEANGGVPALKAIGAPIPQVSFC
PTGGVSISNAPDYLGLPNVVCAGGSWVAPNAAVEAGDWDRIEQLASEAAALSR
>Mature_212_residues
TPQAQSLAAHRIAALAPIIPVLVVNDPAHAQPLASALVKGGLPALEVTLRTPCALEVIAEMAKVEGGVVGAGTLLTPRDV
ENAKEAGAVFGVSPGATAHLIDACAANELPLLPGAATASEVMFLFEQGFDLLKFFPAEANGGVPALKAIGAPIPQVSFCP
TGGVSISNAPDYLGLPNVVCAGGSWVAPNAAVEAGDWDRIEQLASEAAALSR

Specific function: KEY ENZYME IN THE ENTNER-DOUDOROFF PATHWAY. PARTICIPATES IN THE REGULATION OF THE INTRACELLULAR LEVEL OF GLYOXYLATE. [C]

COG id: COG0800

COG function: function code G; 2-keto-3-deoxy-6-phosphogluconate aldolase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the KHG/KDPG aldolase family [H]

Homologues:

Organism=Escherichia coli, GI1788156, Length=180, Percent_Identity=54.4444444444444, Blast_Score=191, Evalue=3e-50,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000887
- InterPro:   IPR013785 [H]

Pfam domain/function: PF01081 Aldolase [H]

EC number: =4.1.3.16; =4.1.2.14 [H]

Molecular weight: Translated: 21459; Mature: 21328

Theoretical pI: Translated: 4.32; Mature: 4.32

Prosite motif: PS00159 ALDOLASE_KDPG_KHG_1 ; PS00160 ALDOLASE_KDPG_KHG_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPQAQSLAAHRIAALAPIIPVLVVNDPAHAQPLASALVKGGLPALEVTLRTPCALEVIA
CCCCHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHCCCCEEEEEECCCHHHHHHH
EMAKVEGGVVGAGTLLTPRDVENAKEAGAVFGVSPGATAHLIDACAANELPLLPGAATAS
HHHHHCCCEECCCCCCCCHHHHHHHHCCCEEECCCCCHHHHHHHHHCCCCCCCCCCCHHH
EVMFLFEQGFDLLKFFPAEANGGVPALKAIGAPIPQVSFCPTGGVSISNAPDYLGLPNVV
HHHHHHHHCCHHHHHCCCCCCCCCHHHHHHCCCCCCCEECCCCCCCCCCCCCCCCCCCEE
CAGGSWVAPNAAVEAGDWDRIEQLASEAAALSR
ECCCCEECCCCCCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TPQAQSLAAHRIAALAPIIPVLVVNDPAHAQPLASALVKGGLPALEVTLRTPCALEVIA
CCCHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHCCCCEEEEEECCCHHHHHHH
EMAKVEGGVVGAGTLLTPRDVENAKEAGAVFGVSPGATAHLIDACAANELPLLPGAATAS
HHHHHCCCEECCCCCCCCHHHHHHHHCCCEEECCCCCHHHHHHHHHCCCCCCCCCCCHHH
EVMFLFEQGFDLLKFFPAEANGGVPALKAIGAPIPQVSFCPTGGVSISNAPDYLGLPNVV
HHHHHHHHCCHHHHHCCCCCCCCCHHHHHHCCCCCCCEECCCCCCCCCCCCCCCCCCCEE
CAGGSWVAPNAAVEAGDWDRIEQLASEAAALSR
ECCCCEECCCCCCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8145647 [H]