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The map label for this gene is ilvI [H]

Identifier: 89054388

GI number: 89054388

Start: 1883652

End: 1885418

Strand: Reverse

Name: ilvI [H]

Synonym: Jann_1897

Alternate gene names: 89054388

Gene position: 1885418-1883652 (Counterclockwise)

Preceding gene: 89054393

Following gene: 89054387

Centisome position: 43.66

GC content: 60.95

Gene sequence:

>1767_bases
ATGTCCCAGAGCACGTCACAGATGACCGGAGCAAAAATGATCGTCGAAGCCCTGAAGGAACAGGGTGTCGACACTGTATT
TGGCTATCCCGGCGGTGCCGTCCTTCCGATCTATGACGAAATCTTCCAGCAAAACGCGATCCGCCACATTCTGGTGCGCC
ACGAACAGGGCGCGGTTCATGCCGCCGAAGGCTATGCGCGGTCCACTGGCAAGCCGGGCGTGGTTCTGGTGACCTCCGGC
CCTGGGGCCACGAATGCGGTGACCGGCCTGACCGACGCGCTGATGGACAGCATCCCGATCGTCTGCCTGACGGGTCAGGT
GCCCACGTTCATGATCGGCTCCGATGCGTTTCAGGAAGCCGACACGGTCGGCATCACACGGCCCTGCACCAAGATGAACT
GGCTAGTGAAGGAAACCGACCGTCTGGCCGACACCATTCACCAGGCCTTCCATATCGCCACGTCTGGCCGCCCCGGTCCC
GTGTTGGTCGACATCCCCAAGGATGTGCAGTTTGCCACCGGCGACTACACCACCAAACCTAAGGCCAAGGTCAGCCACTA
CCAGCCGAAGGTGAAGGGCGACATTGAGATGATCACCCGCCTTGTCGAGGCGATGGAGACCGCTGAACGCCCCCTCTTCT
ATACTGGTGGCGGTGTGATCAATTCGGGCGACCATGCCAGCGCATTGCTGCGGGAGTTGGTGGAGGCCACGGGCTTTCCG
ATCACCTCCACCCTGATGGGTCTGGGCGCTTATCCGGCGTCCGGCGAGAAGTGGATCGGCATGTTGGGGATGCACGGCAC
CTATGAGGCCAATCTGGCCATGCATGGCTGTGACCTGATGATCAACGTCGGTGCGCGGTTCGATGACCGGATCACGGGTC
GGATTGCAGATTTCTCTCCCGGCTCGCGCAAGGGCCACATCGACATCGACCCGTCGTCTATTAACAAAGTCATTCACGCC
GATTTCCCGATCATCGGCGACGTGGGCCACGTGCTGGAAGATATCTTGCGCGTCTGGAAAGCCCGCGGGCGCAAGGCGGA
TCGCACGTCGGTGCAGACCTGGTGGACCCAGATCGAGGCGTGGAAAGCCGTGCACTGCCTTGACTACAAGCCGTCCGAGA
CCACGATCAAACCGCAATACGCGCTGGAACGGCTGGAGGCGCTGACCAAACATCGCAAGGACCGCTTCATCACGACCGAA
GTGGGTCAGCACCAGATGTGGGCCGCGCAGTTTCTGGGCTTTGACGACCCGAACCGCTGGATGACCTCTGGCGGGCTCGG
CACGATGGGCTACGGCGTGCCCGCATCGGTCGGCGTGCAGGTTGCGCATCCCGAGGGGCTGGTGATCAATGTCGCCGGTG
AAGCCTCGTGGATGATGAACATGCAGGAAATGGGAACGGCGGCGCAGTACCGCCTGCCCGTCAAGCAGTTCATCCTGAAT
AACGAACGCCTTGGCATGGTCCGCCAGTGGCAGGAATTGCTCCACGGCGAGCGTTATTCTGAAAGCTGGTCCGAGGCCCT
GCCCGATTTCGTGAAGCTGGCCGAGGCCTTTGGTGCCAAGGGTATCTTGTGCTCCGACCCCAAGGATCTGGACGACGCGA
TCATGGAGATGCTGAATTACGACGGCCCCGTGATCTTCGATTGTCTGGTCGAGAAGCACGAAAACTGCTTCCCGATGATC
CCGTCCGGCAAAGCCCACAATGAAATGCTTCTGGGCGAGGCGGACACAGCGGGCGCCATTGGCGATGCGGGTGGGGTTTT
GGTGTGA

Upstream 100 bases:

>100_bases
ATTGGAACCATGCGCCCCCGGATAACCTCCGGGGGCTTTTTTTTGCATTATATATTCGCACACGACCGATGAAGCGCGAC
ACAGAAGGAACAGGCGCCCC

Downstream 100 bases:

>100_bases
CGTCGTCCGACAAACACGCCCTGCGATCATCCGAGCGTGAAGAATACCTGGAATACAATTTTCTGGGGGCCCTATGCTCA
TATGGGTGGTCGCAGGACAG

Product: acetolactate synthase 3 catalytic subunit

Products: NA

Alternate protein names: AHAS-III; ALS-III; Acetohydroxy-acid synthase III large subunit [H]

Number of amino acids: Translated: 588; Mature: 587

Protein sequence:

>588_residues
MSQSTSQMTGAKMIVEALKEQGVDTVFGYPGGAVLPIYDEIFQQNAIRHILVRHEQGAVHAAEGYARSTGKPGVVLVTSG
PGATNAVTGLTDALMDSIPIVCLTGQVPTFMIGSDAFQEADTVGITRPCTKMNWLVKETDRLADTIHQAFHIATSGRPGP
VLVDIPKDVQFATGDYTTKPKAKVSHYQPKVKGDIEMITRLVEAMETAERPLFYTGGGVINSGDHASALLRELVEATGFP
ITSTLMGLGAYPASGEKWIGMLGMHGTYEANLAMHGCDLMINVGARFDDRITGRIADFSPGSRKGHIDIDPSSINKVIHA
DFPIIGDVGHVLEDILRVWKARGRKADRTSVQTWWTQIEAWKAVHCLDYKPSETTIKPQYALERLEALTKHRKDRFITTE
VGQHQMWAAQFLGFDDPNRWMTSGGLGTMGYGVPASVGVQVAHPEGLVINVAGEASWMMNMQEMGTAAQYRLPVKQFILN
NERLGMVRQWQELLHGERYSESWSEALPDFVKLAEAFGAKGILCSDPKDLDDAIMEMLNYDGPVIFDCLVEKHENCFPMI
PSGKAHNEMLLGEADTAGAIGDAGGVLV

Sequences:

>Translated_588_residues
MSQSTSQMTGAKMIVEALKEQGVDTVFGYPGGAVLPIYDEIFQQNAIRHILVRHEQGAVHAAEGYARSTGKPGVVLVTSG
PGATNAVTGLTDALMDSIPIVCLTGQVPTFMIGSDAFQEADTVGITRPCTKMNWLVKETDRLADTIHQAFHIATSGRPGP
VLVDIPKDVQFATGDYTTKPKAKVSHYQPKVKGDIEMITRLVEAMETAERPLFYTGGGVINSGDHASALLRELVEATGFP
ITSTLMGLGAYPASGEKWIGMLGMHGTYEANLAMHGCDLMINVGARFDDRITGRIADFSPGSRKGHIDIDPSSINKVIHA
DFPIIGDVGHVLEDILRVWKARGRKADRTSVQTWWTQIEAWKAVHCLDYKPSETTIKPQYALERLEALTKHRKDRFITTE
VGQHQMWAAQFLGFDDPNRWMTSGGLGTMGYGVPASVGVQVAHPEGLVINVAGEASWMMNMQEMGTAAQYRLPVKQFILN
NERLGMVRQWQELLHGERYSESWSEALPDFVKLAEAFGAKGILCSDPKDLDDAIMEMLNYDGPVIFDCLVEKHENCFPMI
PSGKAHNEMLLGEADTAGAIGDAGGVLV
>Mature_587_residues
SQSTSQMTGAKMIVEALKEQGVDTVFGYPGGAVLPIYDEIFQQNAIRHILVRHEQGAVHAAEGYARSTGKPGVVLVTSGP
GATNAVTGLTDALMDSIPIVCLTGQVPTFMIGSDAFQEADTVGITRPCTKMNWLVKETDRLADTIHQAFHIATSGRPGPV
LVDIPKDVQFATGDYTTKPKAKVSHYQPKVKGDIEMITRLVEAMETAERPLFYTGGGVINSGDHASALLRELVEATGFPI
TSTLMGLGAYPASGEKWIGMLGMHGTYEANLAMHGCDLMINVGARFDDRITGRIADFSPGSRKGHIDIDPSSINKVIHAD
FPIIGDVGHVLEDILRVWKARGRKADRTSVQTWWTQIEAWKAVHCLDYKPSETTIKPQYALERLEALTKHRKDRFITTEV
GQHQMWAAQFLGFDDPNRWMTSGGLGTMGYGVPASVGVQVAHPEGLVINVAGEASWMMNMQEMGTAAQYRLPVKQFILNN
ERLGMVRQWQELLHGERYSESWSEALPDFVKLAEAFGAKGILCSDPKDLDDAIMEMLNYDGPVIFDCLVEKHENCFPMIP
SGKAHNEMLLGEADTAGAIGDAGGVLV

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=569, Percent_Identity=24.4288224956063, Blast_Score=148, Evalue=2e-35,
Organism=Homo sapiens, GI21361361, Length=602, Percent_Identity=24.5847176079734, Blast_Score=140, Evalue=3e-33,
Organism=Escherichia coli, GI87081685, Length=573, Percent_Identity=48.1675392670157, Blast_Score=556, Evalue=1e-159,
Organism=Escherichia coli, GI1790104, Length=572, Percent_Identity=41.0839160839161, Blast_Score=436, Evalue=1e-123,
Organism=Escherichia coli, GI1786717, Length=565, Percent_Identity=32.7433628318584, Blast_Score=314, Evalue=1e-86,
Organism=Escherichia coli, GI1787096, Length=560, Percent_Identity=27.1428571428571, Blast_Score=180, Evalue=3e-46,
Organism=Escherichia coli, GI1788716, Length=573, Percent_Identity=24.78184991274, Blast_Score=145, Evalue=6e-36,
Organism=Caenorhabditis elegans, GI17531299, Length=570, Percent_Identity=25.0877192982456, Blast_Score=139, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI17531301, Length=570, Percent_Identity=25.0877192982456, Blast_Score=139, Evalue=4e-33,
Organism=Caenorhabditis elegans, GI17542570, Length=531, Percent_Identity=25.4237288135593, Blast_Score=119, Evalue=6e-27,
Organism=Saccharomyces cerevisiae, GI6323755, Length=590, Percent_Identity=44.4067796610169, Blast_Score=486, Evalue=1e-138,
Organism=Saccharomyces cerevisiae, GI6320816, Length=499, Percent_Identity=24.6492985971944, Blast_Score=103, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6321524, Length=545, Percent_Identity=23.6697247706422, Blast_Score=91, Evalue=7e-19,
Organism=Saccharomyces cerevisiae, GI6323073, Length=545, Percent_Identity=24.0366972477064, Blast_Score=89, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6323163, Length=544, Percent_Identity=22.7941176470588, Blast_Score=82, Evalue=2e-16,
Organism=Drosophila melanogaster, GI19922626, Length=549, Percent_Identity=25.8652094717668, Blast_Score=159, Evalue=5e-39,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 64129; Mature: 63998

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQSTSQMTGAKMIVEALKEQGVDTVFGYPGGAVLPIYDEIFQQNAIRHILVRHEQGAVH
CCCCCHHHHHHHHHHHHHHHCCCCEEECCCCCCEEHHHHHHHHHHHHHHHHHCCCCCCEE
AAEGYARSTGKPGVVLVTSGPGATNAVTGLTDALMDSIPIVCLTGQVPTFMIGSDAFQEA
HHCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCEEEEECCCCEEEECCHHHHHH
DTVGITRPCTKMNWLVKETDRLADTIHQAFHIATSGRPGPVLVDIPKDVQFATGDYTTKP
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCEEECCCCCCCC
KAKVSHYQPKVKGDIEMITRLVEAMETAERPLFYTGGGVINSGDHASALLRELVEATGFP
CCHHHCCCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCCHHHHHHHHHHHHCCCC
ITSTLMGLGAYPASGEKWIGMLGMHGTYEANLAMHGCDLMINVGARFDDRITGRIADFSP
HHHHHHHCCCCCCCCCCEEEEECCCCCEECCEEEECCEEEEECCCCCCCCCCCEEECCCC
GSRKGHIDIDPSSINKVIHADFPIIGDVGHVLEDILRVWKARGRKADRTSVQTWWTQIEA
CCCCCEEEECHHHHHHHEECCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
WKAVHCLDYKPSETTIKPQYALERLEALTKHRKDRFITTEVGQHQMWAAQFLGFDDPNRW
HHHEEEECCCCCCCEECHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHCCCCCCCCE
MTSGGLGTMGYGVPASVGVQVAHPEGLVINVAGEASWMMNMQEMGTAAQYRLPVKQFILN
EECCCCCCCCCCCCHHCCEEEECCCCEEEEECCCHHHHHHHHHHCCHHHHCCCHHHHHHC
NERLGMVRQWQELLHGERYSESWSEALPDFVKLAEAFGAKGILCSDPKDLDDAIMEMLNY
CCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHCC
DGPVIFDCLVEKHENCFPMIPSGKAHNEMLLGEADTAGAIGDAGGVLV
CCCEEHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SQSTSQMTGAKMIVEALKEQGVDTVFGYPGGAVLPIYDEIFQQNAIRHILVRHEQGAVH
CCCCHHHHHHHHHHHHHHHCCCCEEECCCCCCEEHHHHHHHHHHHHHHHHHCCCCCCEE
AAEGYARSTGKPGVVLVTSGPGATNAVTGLTDALMDSIPIVCLTGQVPTFMIGSDAFQEA
HHCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCEEEEECCCCEEEECCHHHHHH
DTVGITRPCTKMNWLVKETDRLADTIHQAFHIATSGRPGPVLVDIPKDVQFATGDYTTKP
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCEEECCCCCCCC
KAKVSHYQPKVKGDIEMITRLVEAMETAERPLFYTGGGVINSGDHASALLRELVEATGFP
CCHHHCCCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCCHHHHHHHHHHHHCCCC
ITSTLMGLGAYPASGEKWIGMLGMHGTYEANLAMHGCDLMINVGARFDDRITGRIADFSP
HHHHHHHCCCCCCCCCCEEEEECCCCCEECCEEEECCEEEEECCCCCCCCCCCEEECCCC
GSRKGHIDIDPSSINKVIHADFPIIGDVGHVLEDILRVWKARGRKADRTSVQTWWTQIEA
CCCCCEEEECHHHHHHHEECCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
WKAVHCLDYKPSETTIKPQYALERLEALTKHRKDRFITTEVGQHQMWAAQFLGFDDPNRW
HHHEEEECCCCCCCEECHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHCCCCCCCCE
MTSGGLGTMGYGVPASVGVQVAHPEGLVINVAGEASWMMNMQEMGTAAQYRLPVKQFILN
EECCCCCCCCCCCCHHCCEEEECCCCEEEEECCCHHHHHHHHHHCCHHHHCCCHHHHHHC
NERLGMVRQWQELLHGERYSESWSEALPDFVKLAEAFGAKGILCSDPKDLDDAIMEMLNY
CCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHCC
DGPVIFDCLVEKHENCFPMIPSGKAHNEMLLGEADTAGAIGDAGGVLV
CCCEEHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6308579; 1630901; 9278503; 3891724; 9298646 [H]