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The map label for this gene is lexA

Identifier: 89054365

GI number: 89054365

Start: 1863508

End: 1864194

Strand: Reverse

Name: lexA

Synonym: Jann_1874

Alternate gene names: 89054365

Gene position: 1864194-1863508 (Counterclockwise)

Preceding gene: 89054366

Following gene: 89054363

Centisome position: 43.17

GC content: 63.17

Gene sequence:

>687_bases
ATGCTGACGCGGAAACAACGCGACCTGTTGGAATTTATTCACAAACGGATGCAGCGCGACGGTGTGCCGCCATCGTTTGA
CGAGATGAAAGAGGCGCTGGATTTGCGGTCCAAATCCGGCATCCACCGCCTGATCACAGCGCTGGAGGAGCGCGGGTTTA
TCCGCCGGCTGGCGCACCGAGCCCGCGCGATCGAGATTGTGAAGATGCCCGATGCGATGACGGGCGGCGGGTTTGAGCCC
CGCGTGATCGACGGCGACCGCGGGCCTGCGCCCGCCAATGCGATGGCTGTGCGGGCGGCTCCGGCCCGCGAGGTGCCGGT
GATGGGCCAGATCGCCGCCGGTGTGCCGATTGAAGCGATCAGCCAGGTCGCCTCCCACGTTGCGGTGCCCGAGCAGATGC
TGGGGGCCGGTGGCAACCACTATGCCCTTGAAGTCAAAGGCGATTCGATGATCGATGCCGGCATCAACGACGGCGATATC
GTGGTAATTGAAGAGGGCAACACGGCCGATAACGGCGATATCGTTGTGGCCCTTGTGGAGGATCATGAGGCGACCCTGAA
GCGCCTGCGCCGCAAGGGCGGCATGATCGCACTGGAAGCCGCGAACCCGGCCTATGAAACCCGGGTCTTCCGCGACGATC
AGGTGAAGGTACAGGGCAAACTTGTCGGTCTGATCCGCACCTACTGA

Upstream 100 bases:

>100_bases
ACAGACAGGCCGGAACAAGCGTAGAACATACCCTGAACGGGCACGGAATGTGTCACACCAATGGGCGCAAACCGCCCGGG
ACTATGTGAGGGAGATGGGC

Downstream 100 bases:

>100_bases
AAGACAGCGGGCGGGGCTCCGGCTCTGCCTCATATTTTGCCCTTAGGGGCAAATCACTCCGTCAGCCGGCGGTGGCGCTC
CCGCGCAGATCCCACAGATG

Product: LexA repressor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 228; Mature: 228

Protein sequence:

>228_residues
MLTRKQRDLLEFIHKRMQRDGVPPSFDEMKEALDLRSKSGIHRLITALEERGFIRRLAHRARAIEIVKMPDAMTGGGFEP
RVIDGDRGPAPANAMAVRAAPAREVPVMGQIAAGVPIEAISQVASHVAVPEQMLGAGGNHYALEVKGDSMIDAGINDGDI
VVIEEGNTADNGDIVVALVEDHEATLKRLRRKGGMIALEAANPAYETRVFRDDQVKVQGKLVGLIRTY

Sequences:

>Translated_228_residues
MLTRKQRDLLEFIHKRMQRDGVPPSFDEMKEALDLRSKSGIHRLITALEERGFIRRLAHRARAIEIVKMPDAMTGGGFEP
RVIDGDRGPAPANAMAVRAAPAREVPVMGQIAAGVPIEAISQVASHVAVPEQMLGAGGNHYALEVKGDSMIDAGINDGDI
VVIEEGNTADNGDIVVALVEDHEATLKRLRRKGGMIALEAANPAYETRVFRDDQVKVQGKLVGLIRTY
>Mature_228_residues
MLTRKQRDLLEFIHKRMQRDGVPPSFDEMKEALDLRSKSGIHRLITALEERGFIRRLAHRARAIEIVKMPDAMTGGGFEP
RVIDGDRGPAPANAMAVRAAPAREVPVMGQIAAGVPIEAISQVASHVAVPEQMLGAGGNHYALEVKGDSMIDAGINDGDI
VVIEEGNTADNGDIVVALVEDHEATLKRLRRKGGMIALEAANPAYETRVFRDDQVKVQGKLVGLIRTY

Specific function: Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. In the presence of single-stranded DNA, recA interacts with lexA causing an autocatalytic cleavage which disrupts the DNA-binding part of lexA, lea

COG id: COG1974

COG function: function code KT; SOS-response transcriptional repressors (RecA-mediated autopeptidases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S24 family

Homologues:

Organism=Escherichia coli, GI1790476, Length=228, Percent_Identity=25.8771929824561, Blast_Score=81, Evalue=6e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEXA_JANSC (Q28R71)

Other databases:

- EMBL:   CP000264
- RefSeq:   YP_509816.1
- ProteinModelPortal:   Q28R71
- SMR:   Q28R71
- STRING:   Q28R71
- MEROPS:   S24.001
- GeneID:   3934325
- GenomeReviews:   CP000264_GR
- KEGG:   jan:Jann_1874
- eggNOG:   COG1974
- HOGENOM:   HBG679610
- OMA:   LMFIHER
- PhylomeDB:   Q28R71
- ProtClustDB:   PRK00215
- BioCyc:   JSP290400:JANN_1874-MONOMER
- GO:   GO:0006508
- GO:   GO:0006350
- HAMAP:   MF_00015
- InterPro:   IPR006199
- InterPro:   IPR006200
- InterPro:   IPR006197
- InterPro:   IPR019759
- InterPro:   IPR015927
- InterPro:   IPR011056
- InterPro:   IPR011991
- Gene3D:   G3DSA:2.10.109.10
- Gene3D:   G3DSA:1.10.10.10
- PRINTS:   PR00726
- TIGRFAMs:   TIGR00498

Pfam domain/function: PF01726 LexA_DNA_bind; PF00717 Peptidase_S24; SSF51306 Pept_S24_S26_C

EC number: =3.4.21.88

Molecular weight: Translated: 24819; Mature: 24819

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: NA

Important sites: ACT_SITE 149-149 ACT_SITE 187-187

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTRKQRDLLEFIHKRMQRDGVPPSFDEMKEALDLRSKSGIHRLITALEERGFIRRLAHR
CCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCHHHHHHHHH
ARAIEIVKMPDAMTGGGFEPRVIDGDRGPAPANAMAVRAAPAREVPVMGQIAAGVPIEAI
HHEEEEEECCCCCCCCCCCCEEECCCCCCCCCCCEEEECCCCCCCCCCCHHHCCCCHHHH
SQVASHVAVPEQMLGAGGNHYALEVKGDSMIDAGINDGDIVVIEEGNTADNGDIVVALVE
HHHHHHHCCCHHHHCCCCCEEEEEECCCCEEECCCCCCCEEEEECCCCCCCCCEEEEEEC
DHEATLKRLRRKGGMIALEAANPAYETRVFRDDQVKVQGKLVGLIRTY
CCHHHHHHHHHHCCEEEEECCCCCHHHEEECCCCEEEEEEEEEEEECC
>Mature Secondary Structure
MLTRKQRDLLEFIHKRMQRDGVPPSFDEMKEALDLRSKSGIHRLITALEERGFIRRLAHR
CCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCHHHHHHHHH
ARAIEIVKMPDAMTGGGFEPRVIDGDRGPAPANAMAVRAAPAREVPVMGQIAAGVPIEAI
HHEEEEEECCCCCCCCCCCCEEECCCCCCCCCCCEEEECCCCCCCCCCCHHHCCCCHHHH
SQVASHVAVPEQMLGAGGNHYALEVKGDSMIDAGINDGDIVVIEEGNTADNGDIVVALVE
HHHHHHHCCCHHHHCCCCCEEEEEECCCCEEECCCCCCCEEEEECCCCCCCCCEEEEEEC
DHEATLKRLRRKGGMIALEAANPAYETRVFRDDQVKVQGKLVGLIRTY
CCHHHHHHHHHHCCEEEEECCCCCHHHEEECCCCEEEEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA